BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0004.p (192 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8GI59.1 B8GI59_METPE Isochorismatase hydrolase 209 1e-52 B9E433.1 B9E433_CLOK1 Putative uncharacterized protein 207 4e-52 A5N062.1 A5N062_CLOK5 PhzA EC=3.3.2.1 207 4e-52 Q97TR7.1 Y4030_CLOAB Uncharacterized isochorismatase family prot... 202 1e-50 A6LXB9.1 A6LXB9_CLOB8 Isochorismatase hydrolase 191 2e-47 >B8GI59.1 B8GI59_METPE Isochorismatase hydrolase Length = 198 Score = 209 bits (531), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 102/182 (56%), Positives = 130/182 (71%), Gaps = 1/182 (0%) Query: 8 QKTALVVIDLQKWLGN-QYAPYSAEQVVSNAAVLADAFREQGSMVALVRVSSXXXXXXXX 66 +KTALVVID+QK + N Q+AP+++EQVV NA+ L AF E+G+ V LVRVS+ Sbjct: 15 EKTALVVIDMQKGIVNRQHAPHTSEQVVLNASRLVQAFTEKGAFVVLVRVSTVDGRDMLR 74 Query: 67 XXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREIDTLVLC 126 + P+GWD VPE+ H+ITK+QWGAFYGT+LDLQLRRR IDT+VLC Sbjct: 75 PRTDATTNPVPFPDGWDAFVPELEHFDQVHVITKRQWGAFYGTDLDLQLRRRGIDTIVLC 134 Query: 127 GIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQEIIST 186 GI+TG+GVDTTAREAF GY QIF DAMT ++ EH+YV HIFPRIGR RT++E++ Sbjct: 135 GISTGIGVDTTAREAFQRGYDQIFVEDAMTASTEEEHEYVCTHIFPRIGRIRTSEEVVGL 194 Query: 187 FT 188 + Sbjct: 195 LS 196 >B9E433.1 B9E433_CLOK1 Putative uncharacterized protein Length = 194 Score = 207 bits (527), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 102/183 (55%), Positives = 126/183 (68%), Gaps = 1/183 (0%) Query: 3 KLFDPQKTALVVIDLQKWLGN-QYAPYSAEQVVSNAAVLADAFREQGSMVALVRVSSXXX 61 +L +KTALV+IDLQ + N + +P++ +VV NA+ L +AF E+G+ V LVRVSS Sbjct: 10 ELIKSEKTALVIIDLQNGIVNRELSPHTGVEVVQNASRLINAFTEKGAFVVLVRVSSIDG 69 Query: 62 XXXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREID 121 PEGWD VPE+ H ITK+QWGAFYGT+LDLQLRRR ID Sbjct: 70 KDMLKPSVDFKINPTQFPEGWDSYVPEIANNKNVHTITKRQWGAFYGTDLDLQLRRRGID 129 Query: 122 TLVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQ 181 T+VLCGI+TG+GVDTTAREAF HGY QIF DAMT S+ EHDYV HIFPRIG+ R ++ Sbjct: 130 TIVLCGISTGIGVDTTAREAFQHGYNQIFVEDAMTAGSKEEHDYVCKHIFPRIGKLRRSE 189 Query: 182 EII 184 EI+ Sbjct: 190 EIV 192 >A5N062.1 A5N062_CLOK5 PhzA EC=3.3.2.1 Length = 194 Score = 207 bits (527), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 102/183 (55%), Positives = 126/183 (68%), Gaps = 1/183 (0%) Query: 3 KLFDPQKTALVVIDLQKWLGN-QYAPYSAEQVVSNAAVLADAFREQGSMVALVRVSSXXX 61 +L +KTALV+IDLQ + N + +P++ +VV NA+ L +AF E+G+ V LVRVSS Sbjct: 10 ELIKSEKTALVIIDLQNGIVNRELSPHTGVEVVQNASRLINAFTEKGAFVVLVRVSSIDG 69 Query: 62 XXXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRREID 121 PEGWD VPE+ H ITK+QWGAFYGT+LDLQLRRR ID Sbjct: 70 KDMLKPSVDFKINPTQFPEGWDSYVPEIANNKNVHTITKRQWGAFYGTDLDLQLRRRGID 129 Query: 122 TLVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRTTQ 181 T+VLCGI+TG+GVDTTAREAF HGY QIF DAMT S+ EHDYV HIFPRIG+ R ++ Sbjct: 130 TIVLCGISTGIGVDTTAREAFQHGYNQIFVEDAMTAGSKEEHDYVCKHIFPRIGKLRRSE 189 Query: 182 EII 184 EI+ Sbjct: 190 EIV 192 >Q97TR7.1 Y4030_CLOAB Uncharacterized isochorismatase family protein CA_P0030 EC=3.-.-.- Length = 187 Score = 202 bits (514), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 101/185 (54%), Positives = 129/185 (69%), Gaps = 2/185 (1%) Query: 1 MEKLFDPQKTALVVIDLQKWL-GNQYAPYSAEQVVSNAAVLADAFREQGSMVALVRVSSX 59 ME L + +KTALVVIDLQK + ++APY+ ++VV A+ L AF ++G+ V LV+VS+ Sbjct: 1 MENL-EIEKTALVVIDLQKGIVAGEHAPYTGKEVVEKASKLVKAFTDKGAFVVLVKVSTI 59 Query: 60 XXXXXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRRE 119 + + PEGWD VPE+ + ITK+QWGAFYGT+LDLQLRRR Sbjct: 60 DGKDMLKPKLDSEINQIKRPEGWDSYVPEISNIKNTYAITKRQWGAFYGTDLDLQLRRRG 119 Query: 120 IDTLVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTRT 179 IDT+VLCGI+TG+GVDTTAREAF HGY QIF DAMT S+ EH YV +IFPR+GR R Sbjct: 120 IDTMVLCGISTGIGVDTTAREAFQHGYNQIFVEDAMTARSREEHAYVCKYIFPRLGRIRN 179 Query: 180 TQEII 184 T+EI+ Sbjct: 180 TEEIL 184 >A6LXB9.1 A6LXB9_CLOB8 Isochorismatase hydrolase Length = 198 Score = 191 bits (486), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 93/187 (49%), Positives = 127/187 (67%), Gaps = 3/187 (1%) Query: 2 EKLFDPQKTALVVIDLQKWLGNQYA---PYSAEQVVSNAAVLADAFREQGSMVALVRVSS 58 ++L + + +ALV+IDLQK + P+ +QV+ NA+ L F E+G+ + LVRVS+ Sbjct: 9 KELINAKTSALVLIDLQKGIAGGGISGSPHLMDQVIQNASKLVKEFGEKGAFIVLVRVST 68 Query: 59 XXXXXXXXXXXXTPAPALNLPEGWDEIVPEMRVAATDHIITKKQWGAFYGTELDLQLRRR 118 + + EGWDE+VPE+ HI+TK+QWGAFYGT+LDLQLRRR Sbjct: 69 VDGKDMVKPKTDLASSGMKYQEGWDELVPEIAGTKNVHIVTKRQWGAFYGTDLDLQLRRR 128 Query: 119 EIDTLVLCGIATGLGVDTTAREAFMHGYQQIFAIDAMTGFSQAEHDYVKGHIFPRIGRTR 178 IDT+VL GI+T +GVDTTAREAF HGY QIF DAMT ++ EH+YV +IFPRIG+ R Sbjct: 129 GIDTIVLGGISTSIGVDTTAREAFQHGYNQIFLEDAMTAVTKEEHEYVCKYIFPRIGKIR 188 Query: 179 TTQEIIS 185 T++++IS Sbjct: 189 TSEQVIS 195 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 980,883,616 Number of extensions: 33148000 Number of successful extensions: 75206 Number of sequences better than 1.0e-05: 718 Number of HSP's gapped: 74797 Number of HSP's successfully gapped: 718 Length of query: 192 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 64 Effective length of database: 1,854,806,283 Effective search space: 118707602112 Effective search space used: 118707602112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)