BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0091.0001.p (415 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7K7.1 B3K7K7_9BACI Serine hydroxymethyltransferase EC=2.1.2.1 759 0.0 B1DEI6.1 B1DEI6_9BACL Serine hydroxymethyltransferase EC=2.1.2.1 733 0.0 B7GMG4.1 GLYA_ANOFW Serine hydroxymethyltransferase EC=2.1.2.1 622 e-176 C0Z819.1 C0Z819_BREBN Serine hydroxymethyltransferase EC=2.1.2.1 619 e-175 Q65DW5.1 GLYA_BACLD Serine hydroxymethyltransferase EC=2.1.2.1 615 e-174 >B3K7K7.1 B3K7K7_9BACI Serine hydroxymethyltransferase EC=2.1.2.1 Length = 416 Score = 759 bits (1959), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/415 (87%), Positives = 389/415 (93%) Query: 1 MEQLRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRY 60 ME LRK+DPAVLEAM LEL RQR+NIELIASENIVSEAVMEAMG+VLTNKYAEGYPGKRY Sbjct: 2 MEHLRKNDPAVLEAMDLELKRQRSNIELIASENIVSEAVMEAMGTVLTNKYAEGYPGKRY 61 Query: 61 YGGCEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHG 120 YGGCE VDIVE++ARDRAK+LFGA+HANVQPHSGAQANMAVYLAAL PGDTVLGMNLAHG Sbjct: 62 YGGCERVDIVEDIARDRAKELFGAEHANVQPHSGAQANMAVYLAALKPGDTVLGMNLAHG 121 Query: 121 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDF 180 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRP+LIVAGASAYPR IDF Sbjct: 122 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPRLIVAGASAYPRIIDF 181 Query: 181 EALGSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCRQ 240 E L +IANDVGALFMVDMAHIAGLVAAGLHP+PVPHAHFVTTTTHKTLRGPRGGMILC++ Sbjct: 182 EKLAAIANDVGALFMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCKK 241 Query: 241 PWAAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEG 300 WA AIDKAVFPGSQGGPLMHVIASKAV+ GEAL PSF+TYAENVVKNAKVLA+TLI EG Sbjct: 242 AWAQAIDKAVFPGSQGGPLMHVIASKAVALGEALDPSFKTYAENVVKNAKVLADTLIEEG 301 Query: 301 VNIVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT 360 +NIVSGGTDNHLML+DTRNL+ITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT Sbjct: 302 LNIVSGGTDNHLMLVDTRNLDITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT 361 Query: 361 PAVTSRGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIYPGLQY 415 PAVTSRGM+EQAMV I +IIA LK PKDE L +A R VA LT+QYP+Y ++Y Sbjct: 362 PAVTSRGMDEQAMVKIAKIIAMTLKQPKDEATLEKAGRLVAELTDQYPLYAEMKY 416 >B1DEI6.1 B1DEI6_9BACL Serine hydroxymethyltransferase EC=2.1.2.1 Length = 415 Score = 733 bits (1893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/415 (83%), Positives = 381/415 (91%) Query: 1 MEQLRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRY 60 ME LR DP VL+AMGLEL RQR NIELIASENIVSEAV+EAMGSVLTNKYAEGYPGKR+ Sbjct: 1 MENLRNQDPEVLKAMGLELQRQRDNIELIASENIVSEAVLEAMGSVLTNKYAEGYPGKRF 60 Query: 61 YGGCEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHG 120 YGGCE VDIVE++AR+RAK++FGA+HANVQPHSGAQANMAVYLA L PGDTVLGMNLAHG Sbjct: 61 YGGCEHVDIVEDIARERAKEIFGAEHANVQPHSGAQANMAVYLAVLKPGDTVLGMNLAHG 120 Query: 121 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDF 180 GHLTHGSPVNASGLLYNFVAYGV+EDTF I+Y+EVRKAAFKHRP+LIVAGASAYPRTIDF Sbjct: 121 GHLTHGSPVNASGLLYNFVAYGVEEDTFTINYEEVRKAAFKHRPRLIVAGASAYPRTIDF 180 Query: 181 EALGSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCRQ 240 E LG IA DVGALFMVDMAHIAGLVAAGLHP+PVPHAHFVTTTTHKTLRGPRGGMILCR+ Sbjct: 181 EKLGQIAQDVGALFMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCRK 240 Query: 241 PWAAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEG 300 PWAAAIDKAVFPGSQGGPLMH+IA+KAV+FGEALQPSF+ YA+ VV NAKVL+ETLI EG Sbjct: 241 PWAAAIDKAVFPGSQGGPLMHIIAAKAVAFGEALQPSFKEYAQKVVSNAKVLSETLIAEG 300 Query: 301 VNIVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT 360 +NIVSGGTDNHLML+DTR+LNI+G+DAE VLDSIGIT NKNAIPFDPTSPFVTSGIR+GT Sbjct: 301 INIVSGGTDNHLMLIDTRSLNISGRDAEHVLDSIGITANKNAIPFDPTSPFVTSGIRLGT 360 Query: 361 PAVTSRGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIYPGLQY 415 PA TSRGM+E AM TIG IIA LK+PKDE LA+A V LT QYP+YP ++Y Sbjct: 361 PAATSRGMDESAMKTIGEIIAMTLKSPKDEAVLAKATGMVRDLTAQYPLYPNMKY 415 >B7GMG4.1 GLYA_ANOFW Serine hydroxymethyltransferase EC=2.1.2.1 Length = 413 Score = 622 bits (1604), Expect = e-176, Method: Compositional matrix adjust. Identities = 297/410 (72%), Positives = 341/410 (83%) Query: 1 MEQLRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRY 60 M +L + DP V +A+ EL RQ+ IELIASEN VSEAVMEA GSVLTNKYAEGYPG+RY Sbjct: 3 MSRLSQQDPQVFQAIQDELKRQQTKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGRRY 62 Query: 61 YGGCEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHG 120 YGGCE VD+VE LAR+RAKQLFGA+HANVQPHSGAQANMAVY L GDTVLGMNL+HG Sbjct: 63 YGGCEHVDVVEELARERAKQLFGAEHANVQPHSGAQANMAVYFTILQHGDTVLGMNLSHG 122 Query: 121 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDF 180 GHLTHGSPVN SG+ YNF+ YGV +T I+YD+VR+ A KH+PKLIVAGASAYPRTIDF Sbjct: 123 GHLTHGSPVNFSGIQYNFIEYGVDPETHRINYDDVREKALKHKPKLIVAGASAYPRTIDF 182 Query: 181 EALGSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCRQ 240 IA++VGA FMVDMAHIAGLVAAGLHP+PVP+AHFVTTTTHKTLRGPRGGMILC++ Sbjct: 183 AKFREIADEVGAYFMVDMAHIAGLVAAGLHPNPVPYAHFVTTTTHKTLRGPRGGMILCQE 242 Query: 241 PWAAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEG 300 +A IDKA+FPG QGGPLMHVIA+KAV+ GEALQ F+TYA+N+V NAK LAE L+ EG Sbjct: 243 QFAKQIDKAIFPGIQGGPLMHVIAAKAVALGEALQDDFKTYAQNIVNNAKRLAEALVAEG 302 Query: 301 VNIVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT 360 +VSGGTDNHL+L+D R++ +TGK AEKVLD IGITVNKN IP+DP SPFVTSGIRIGT Sbjct: 303 FTLVSGGTDNHLLLIDLRSIGLTGKVAEKVLDEIGITVNKNTIPYDPESPFVTSGIRIGT 362 Query: 361 PAVTSRGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIY 410 AVTSRG + M I RII+ LK+ DE KL EA R VAALTE++P+Y Sbjct: 363 AAVTSRGFGLEEMDEIARIISIALKHKDDEQKLDEARRRVAALTEKFPLY 412 >C0Z819.1 C0Z819_BREBN Serine hydroxymethyltransferase EC=2.1.2.1 Length = 416 Score = 619 bits (1596), Expect = e-175, Method: Compositional matrix adjust. Identities = 290/413 (70%), Positives = 342/413 (82%) Query: 1 MEQLRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRY 60 ++ LRK DP V+EA+ LEL RQR IELIASEN VS AVMEAMG+VLTNKYAEGYPG+RY Sbjct: 2 LDFLRKQDPQVMEAVQLELGRQRDKIELIASENFVSRAVMEAMGTVLTNKYAEGYPGRRY 61 Query: 61 YGGCEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHG 120 YGGCE VDIVEN+ARDR K++FGA+HANVQPHSGAQANMAVY L PGDTVLGMNL+HG Sbjct: 62 YGGCEYVDIVENIARDRVKEIFGAEHANVQPHSGAQANMAVYFTILQPGDTVLGMNLSHG 121 Query: 121 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDF 180 GHLTHGS VN SG LYNFV YGV EDT LI+Y++VR A +H+PKLIVAGASAYPRTIDF Sbjct: 122 GHLTHGSAVNFSGTLYNFVDYGVDEDTHLINYEDVRAKALEHKPKLIVAGASAYPRTIDF 181 Query: 181 EALGSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCRQ 240 IA++VGA FMVDMAHIAGLVAAGLHP+PVPHAHFVT+TTHKTLRGPRGG+ILC++ Sbjct: 182 AKFREIADEVGAYFMVDMAHIAGLVAAGLHPNPVPHAHFVTSTTHKTLRGPRGGLILCKE 241 Query: 241 PWAAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEG 300 +A IDK+VFPG QGGPLMHVIA+KAV+FGE LQP F+ YA ++KNA+ AE+L EG Sbjct: 242 EFAKGIDKSVFPGVQGGPLMHVIAAKAVAFGENLQPEFKDYAARIIKNARAFAESLTAEG 301 Query: 301 VNIVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT 360 + +VSGGTDNHL+L+D + +TGK AE +LD + IT NKN IP+D SPFVTSG+R+GT Sbjct: 302 LTLVSGGTDNHLVLIDVSKIGLTGKVAEHLLDEVSITTNKNTIPYDTQSPFVTSGVRMGT 361 Query: 361 PAVTSRGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIYPGL 413 PAVTSRG +E+AM + IIA LKNP+D K EA + VAAL +++P+Y GL Sbjct: 362 PAVTSRGFDEEAMKEVAAIIALTLKNPEDAAKHEEARQRVAALCQRFPMYEGL 414 >Q65DW5.1 GLYA_BACLD Serine hydroxymethyltransferase EC=2.1.2.1 Length = 415 Score = 615 bits (1585), Expect = e-174, Method: Compositional matrix adjust. Identities = 295/415 (71%), Positives = 342/415 (82%) Query: 1 MEQLRKSDPAVLEAMGLELSRQRANIELIASENIVSEAVMEAMGSVLTNKYAEGYPGKRY 60 M+ L D V A+ E RQ++ IELIASEN VSEAVMEA GSVLTNKYAEGYPGKRY Sbjct: 1 MKHLPAQDEQVFSAIQDERKRQQSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRY 60 Query: 61 YGGCEDVDIVENLARDRAKQLFGADHANVQPHSGAQANMAVYLAALNPGDTVLGMNLAHG 120 YGGCE VD+ E++ARDRAKQ+FGA+H NVQPHSGAQANMAVY L GDTVLGMNLAHG Sbjct: 61 YGGCEHVDVAEDIARDRAKQIFGAEHVNVQPHSGAQANMAVYFTILEHGDTVLGMNLAHG 120 Query: 121 GHLTHGSPVNASGLLYNFVAYGVQEDTFLIDYDEVRKAAFKHRPKLIVAGASAYPRTIDF 180 GHLTHGSPVN SG+ YNFV YGV ++T IDY++VR+ A KH+PKLIVAGASAYPRTIDF Sbjct: 121 GHLTHGSPVNFSGVQYNFVEYGVDKETQYIDYEDVREKALKHKPKLIVAGASAYPRTIDF 180 Query: 181 EALGSIANDVGALFMVDMAHIAGLVAAGLHPSPVPHAHFVTTTTHKTLRGPRGGMILCRQ 240 + IA++VGA MVDMAHIAGLVAAGLHP+PVP+A FVTTTTHKTLRGPRGGMILCR+ Sbjct: 181 KKFREIADEVGAYVMVDMAHIAGLVAAGLHPNPVPYADFVTTTTHKTLRGPRGGMILCRE 240 Query: 241 PWAAAIDKAVFPGSQGGPLMHVIASKAVSFGEALQPSFRTYAENVVKNAKVLAETLIGEG 300 +A IDK++FPG QGGPLMHVIA+KAVSFGEAL+ F+TYA+NV+ NAK LAETL EG Sbjct: 241 EFAKQIDKSIFPGIQGGPLMHVIAAKAVSFGEALKDEFKTYAQNVINNAKRLAETLKKEG 300 Query: 301 VNIVSGGTDNHLMLLDTRNLNITGKDAEKVLDSIGITVNKNAIPFDPTSPFVTSGIRIGT 360 + +VSGGTDNHL+L+D R+L ITGK AE VLD +GITVNKNAIP+DP PFVTSGIR+GT Sbjct: 301 IELVSGGTDNHLVLVDLRSLGITGKVAENVLDEVGITVNKNAIPYDPEKPFVTSGIRVGT 360 Query: 361 PAVTSRGMNEQAMVTIGRIIASVLKNPKDEGKLAEAAREVAALTEQYPIYPGLQY 415 AVTSRG + +A+ +G IIA LKN +DE KL EA + V ALT ++P+Y GL Y Sbjct: 361 AAVTSRGFDLEAIEEVGAIIALALKNHEDEAKLEEAKQRVEALTNRFPLYTGLDY 415 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,466,957,177 Number of extensions: 105073364 Number of successful extensions: 287541 Number of sequences better than 1.0e-05: 2264 Number of HSP's gapped: 283232 Number of HSP's successfully gapped: 2298 Length of query: 415 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 277 Effective length of database: 1,760,596,133 Effective search space: 487685128841 Effective search space used: 487685128841 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)