BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0088.0004.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0TFK7.1 B0TFK7_HELMI Branched-chain amino acid abc transporter,... 318 4e-85 B4BMX0.1 B4BMX0_9BACI Inner-membrane translocator 318 4e-85 A4ISG9.1 A4ISG9_GEOTN Branched-chain amino acid ABC transporter ... 318 5e-85 A4J6P8.1 A4J6P8_DESRM Inner-membrane translocator 315 2e-84 Q5KVM3.1 Q5KVM3_GEOKA Branched-chain amino acid ABC transporter ... 314 8e-84 >B0TFK7.1 B0TFK7_HELMI Branched-chain amino acid abc transporter, permease protein Length = 330 Score = 318 bits (816), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 171/323 (52%), Positives = 219/323 (67%), Gaps = 9/323 (2%) Query: 1 VKKL-NTNFWVGIIVALAFYGIVQVLLTTGIFSDVTRSMLLLIGVNIMLAVSLNLITGIT 59 VKKL N + V L FYG V L G D + L LI +NI+LA SLN+I G+T Sbjct: 4 VKKLLNLPNLIVAAVLLLFYGFVSFGLELGFIDDYYKLNLFLICINIILATSLNIINGMT 63 Query: 60 GQFSIGHAGFMSVGAYTSAILTLDYNVPFIPAILAGGVLAAVFGVLIGMPTLRLNGDYLA 119 GQFSIGHAGFM++GAY SAI+T+ PF IL G + AA+ G L+GMPTLRL GDYLA Sbjct: 64 GQFSIGHAGFMAIGAYGSAIMTMKLGYPFPVGILVGALAAAIAGFLVGMPTLRLKGDYLA 123 Query: 120 IATLGFGEIIRIIMLNTEYVGGASGLSGIPAKTTWTMLFLFTLITVVVINNFIGSTHGRX 179 IATLGFGEIIR+I+LN EYVGGA+GL+GI T+W LF T+ TV++I NF+ STHGR Sbjct: 124 IATLGFGEIIRVIILNIEYVGGAAGLNGISQFTSWEWLFFLTVATVLIIKNFMTSTHGRA 183 Query: 180 XXXXXXXXXXXXXMGINTTRYKIIAFTIGALFAGMAGGLSAHTFYVITPGSFNFLKSFEI 239 MGI+ TRYK+IAFT+GA FAG+AG L AH FY I P +F F+KSF+I Sbjct: 184 CIAIRENEIAAETMGIDITRYKVIAFTMGAFFAGIAGSLHAHYFYTIQPTTFGFMKSFDI 243 Query: 240 IVMVVLGGLGSTAGSIVGAVFVTLLYTYLREFPEWRMIIYSIVLILMMIFRPSGLLGKTK 299 +V VVLGGLGS GS++ A+ +T+L +L+E+ E RM+IYS++L+++M+ RP GL+G + Sbjct: 244 LVFVVLGGLGSLTGSVISAIALTILSAFLQEYAELRMVIYSLLLVIVMLVRPQGLMGTAE 303 Query: 300 FSLGKL--------GKKEAKAND 314 FS + GK ND Sbjct: 304 FSYAGMRRLYARLTGKGRGAGND 326 >B4BMX0.1 B4BMX0_9BACI Inner-membrane translocator Length = 324 Score = 318 bits (815), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 162/309 (52%), Positives = 221/309 (71%), Gaps = 1/309 (0%) Query: 3 KLNTNFWVGIIVALAFYGIVQVLLTTGIFSDVTRSMLLLIGVNIMLAVSLNLITGITGQF 62 K FWV II+A AF+ +VQ L+T+G + + L + +N++LA SL+LI GITGQF Sbjct: 5 KRTRGFWVSIILAFAFFAVVQWLITSGTLNIFYVNTLFFMAINVILAASLHLIIGITGQF 64 Query: 63 SIGHAGFMSVGAYTSAILTLDYNVPFIPAILAGGVLAAVFGVLIGMPTLRLNGDYLAIAT 122 SIGHAGF +VGAY SAI+T+ +PF +L GV A + G++IG+P+LRL GDYLAIAT Sbjct: 65 SIGHAGFFAVGAYASAIMTMKLQLPFAVGVLVAGVAAMLAGLIIGVPSLRLKGDYLAIAT 124 Query: 123 LGFGEIIRIIMLNTEYVGGASGLSGIPAKTTWTMLFLFTLITVVVINNFIGSTHGRXXXX 182 LGFGEI+RI++LN +YVGGASG++ + TTW +F LIT++VI NF STHGR Sbjct: 125 LGFGEIVRIVLLNIDYVGGASGMT-VTHMTTWPWVFACLLITIIVIANFTNSTHGRACIS 183 Query: 183 XXXXXXXXXXMGINTTRYKIIAFTIGALFAGMAGGLSAHTFYVITPGSFNFLKSFEIIVM 242 MGINTT YK+ AF IG+ FAG+AG L AH FY+I P +F FLKSF+I++ Sbjct: 184 IREDEIAADAMGINTTYYKVAAFAIGSFFAGIAGALYAHHFYIIQPSNFGFLKSFDILIF 243 Query: 243 VVLGGLGSTAGSIVGAVFVTLLYTYLREFPEWRMIIYSIVLILMMIFRPSGLLGKTKFSL 302 VVLGGLGS +G+ + AV +T++ T+L+ +PE RMIIYS+VLI +M++RP+GL+GK + + Sbjct: 244 VVLGGLGSLSGATISAVLLTIVSTFLQNYPETRMIIYSLVLIFVMLYRPTGLMGKKELTS 303 Query: 303 GKLGKKEAK 311 +K A+ Sbjct: 304 LLKWRKTAQ 312 >A4ISG9.1 A4ISG9_GEOTN Branched-chain amino acid ABC transporter (Permease) Length = 328 Score = 318 bits (815), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 162/309 (52%), Positives = 221/309 (71%), Gaps = 1/309 (0%) Query: 3 KLNTNFWVGIIVALAFYGIVQVLLTTGIFSDVTRSMLLLIGVNIMLAVSLNLITGITGQF 62 K FWV II+A AF+ +VQ L+T+G + + L + +N++LA SL+LI GITGQF Sbjct: 9 KRTRGFWVSIILAFAFFAVVQWLITSGTLNIFYVNTLFFMAINVILAASLHLIIGITGQF 68 Query: 63 SIGHAGFMSVGAYTSAILTLDYNVPFIPAILAGGVLAAVFGVLIGMPTLRLNGDYLAIAT 122 SIGHAGF +VGAY SAI+T+ +PF +L GV A + G++IG+P+LRL GDYLAIAT Sbjct: 69 SIGHAGFFAVGAYASAIMTMKLQLPFAVGVLVAGVAAMLAGLIIGVPSLRLKGDYLAIAT 128 Query: 123 LGFGEIIRIIMLNTEYVGGASGLSGIPAKTTWTMLFLFTLITVVVINNFIGSTHGRXXXX 182 LGFGEI+RI++LN +YVGGASG++ + TTW +F LIT++VI NF STHGR Sbjct: 129 LGFGEIVRIVLLNIDYVGGASGMT-VTHMTTWPWVFACLLITIIVIANFTNSTHGRACIS 187 Query: 183 XXXXXXXXXXMGINTTRYKIIAFTIGALFAGMAGGLSAHTFYVITPGSFNFLKSFEIIVM 242 MGINTT YK+ AF IG+ FAG+AG L AH FY+I P +F FLKSF+I++ Sbjct: 188 IREDEIAADAMGINTTYYKVAAFAIGSFFAGIAGALYAHHFYIIQPSNFGFLKSFDILIF 247 Query: 243 VVLGGLGSTAGSIVGAVFVTLLYTYLREFPEWRMIIYSIVLILMMIFRPSGLLGKTKFSL 302 VVLGGLGS +G+ + AV +T++ T+L+ +PE RMIIYS+VLI +M++RP+GL+GK + + Sbjct: 248 VVLGGLGSLSGATISAVLLTIVSTFLQNYPETRMIIYSLVLIFVMLYRPTGLMGKKELTS 307 Query: 303 GKLGKKEAK 311 +K A+ Sbjct: 308 LLKWRKTAQ 316 >A4J6P8.1 A4J6P8_DESRM Inner-membrane translocator Length = 326 Score = 315 bits (808), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 169/305 (55%), Positives = 222/305 (72%) Query: 16 LAFYGIVQVLLTTGIFSDVTRSMLLLIGVNIMLAVSLNLITGITGQFSIGHAGFMSVGAY 75 LA +G V +L+ TGI + L+LIG+NI+LAVSLNLI G GQFSIGHAGFM++GAY Sbjct: 19 LAIWGSVYLLIQTGIINPFYELNLILIGINIILAVSLNLINGFNGQFSIGHAGFMAIGAY 78 Query: 76 TSAILTLDYNVPFIPAILAGGVLAAVFGVLIGMPTLRLNGDYLAIATLGFGEIIRIIMLN 135 +AI+T+ +PF A+L G V+A +FG+L+G+PTLRL GDYLAIATLGFGEIIR +++N Sbjct: 79 GAAIITIKAGLPFPVALLFGAVVAGIFGILVGLPTLRLRGDYLAIATLGFGEIIRGVIVN 138 Query: 136 TEYVGGASGLSGIPAKTTWTMLFLFTLITVVVINNFIGSTHGRXXXXXXXXXXXXXXMGI 195 +Y+GGA GLSGIP WT +F T++TVV+I NFI STHGR MGI Sbjct: 139 IDYIGGAYGLSGIPKMANWTWVFWLTVLTVVIITNFINSTHGRATVSIRENEIAAEAMGI 198 Query: 196 NTTRYKIIAFTIGALFAGMAGGLSAHTFYVITPGSFNFLKSFEIIVMVVLGGLGSTAGSI 255 NTT+YK++AFTIG+ FAG+AGGL AH FY I PG+FNFLKSF+I+VMVVLGG GS GSI Sbjct: 199 NTTKYKVMAFTIGSFFAGLAGGLHAHYFYTIQPGTFNFLKSFDILVMVVLGGQGSITGSI 258 Query: 256 VGAVFVTLLYTYLREFPEWRMIIYSIVLILMMIFRPSGLLGKTKFSLGKLGKKEAKANDA 315 + A+ +T+L L+ R++IY+++LI++MIFRP GL+G +FSL G+K + Sbjct: 259 IAAIGLTILSASLQSLVSLRLVIYALLLIIIMIFRPQGLMGTKEFSLSVFGEKSKDKGEK 318 Query: 316 ISESS 320 +S Sbjct: 319 QHGTS 323 >Q5KVM3.1 Q5KVM3_GEOKA Branched-chain amino acid ABC transporter (Permease) Length = 324 Score = 314 bits (804), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 159/309 (51%), Positives = 219/309 (70%), Gaps = 1/309 (0%) Query: 3 KLNTNFWVGIIVALAFYGIVQVLLTTGIFSDVTRSMLLLIGVNIMLAVSLNLITGITGQF 62 K FWV +I+A AF+ +V+ L+ +G + + L + +N+MLA SL+LI GITGQF Sbjct: 5 KRTRGFWVSVILAFAFFAVVEWLIASGTLNVFYVNTLFFMAINVMLAASLHLIIGITGQF 64 Query: 63 SIGHAGFMSVGAYTSAILTLDYNVPFIPAILAGGVLAAVFGVLIGMPTLRLNGDYLAIAT 122 SIGHAGF +VGAY SA++T+ +PF +L G+ A + G++IG+P+LRL GDYLAIAT Sbjct: 65 SIGHAGFFAVGAYASAVMTMKLQLPFAVGVLTAGIAAMLAGLVIGVPSLRLKGDYLAIAT 124 Query: 123 LGFGEIIRIIMLNTEYVGGASGLSGIPAKTTWTMLFLFTLITVVVINNFIGSTHGRXXXX 182 LGFGEI+RI +LN +YVGGASG++ + TTW +F +T++VI NF STHGR Sbjct: 125 LGFGEIVRIALLNIDYVGGASGMT-VTHMTTWPWVFACLFVTILVIANFTNSTHGRACIS 183 Query: 183 XXXXXXXXXXMGINTTRYKIIAFTIGALFAGMAGGLSAHTFYVITPGSFNFLKSFEIIVM 242 MGINTT YK+ AF IG+ FAG+AG L AH FY+I P +F FLKSF+I++ Sbjct: 184 IREDEIAADAMGINTTYYKVAAFAIGSFFAGIAGALYAHHFYIIQPSNFGFLKSFDILIF 243 Query: 243 VVLGGLGSTAGSIVGAVFVTLLYTYLREFPEWRMIIYSIVLILMMIFRPSGLLGKTKFSL 302 VVLGGLGS +G++V AV +T++ T+L+ +PE RMIIYSIVLIL+M++RP+GL+G + S Sbjct: 244 VVLGGLGSLSGAVVAAVLLTIVSTFLQNYPETRMIIYSIVLILVMLYRPTGLMGTKELSS 303 Query: 303 GKLGKKEAK 311 +K A+ Sbjct: 304 LFKWRKAAQ 312 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.143 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,704,455,522 Number of extensions: 68027387 Number of successful extensions: 350515 Number of sequences better than 1.0e-05: 4134 Number of HSP's gapped: 344879 Number of HSP's successfully gapped: 4318 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)