BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0010.p (423 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z5D2.1 C0Z5D2_BREBN Putative chromate transport protein 436 e-120 Q62NX4.1 Q62NX4_BACLD Chromate transporter 428 e-118 Q65DF4.1 Q65DF4_BACLD Putative uncharacterized protein 428 e-118 Q6HIU6.1 Q6HIU6_BACHK Chromate transporter 427 e-118 Q8ELF3.1 Q8ELF3_OCEIH Chromate transporter 427 e-117 >C0Z5D2.1 C0Z5D2_BREBN Putative chromate transport protein Length = 411 Score = 436 bits (1121), Expect = e-120, Method: Compositional matrix adjust. Identities = 239/420 (56%), Positives = 280/420 (66%), Gaps = 10/420 (2%) Query: 1 MERRALNEAESTGRGRNLGMETGSQRPVEXXXXXXXXXXLLEVLGVSLKLGLTSFGGPIA 60 M +R E S + NL E G Q+ ++EVL VS KLG+TSFGGPIA Sbjct: 1 MMKRKAKEVYSKVK-NNLREEAGHQK--------SSWKSVMEVLLVSSKLGVTSFGGPIA 51 Query: 61 HLGYFHNEYIRRRKWMDERSYADLVALCQFLPGPASSQVGIGIGVVRAGLLGGFIAWLGF 120 HLGYFHNEYIRRRKWMDE+SYADLVALCQFLPGPASSQVGIGIG++RAGL GG AWLGF Sbjct: 52 HLGYFHNEYIRRRKWMDEQSYADLVALCQFLPGPASSQVGIGIGIMRAGLWGGVAAWLGF 111 Query: 121 TLPSVIALAVFALVLQGVDIAGAGWIHGLKXXXXXXXXXXXLGMGQKLTPDRGRVTIAVI 180 T+PSV+AL +FA LQGVD A W+HGLK GMGQKL PDR RVTIA+ Sbjct: 112 TVPSVLALLIFAFTLQGVDSGSASWVHGLKLVAVAIVAHAVWGMGQKLAPDRTRVTIAIT 171 Query: 181 AAVFALSWHXXXXXXXXXXXXGVIGLWLYR-GQPDRGPDLPIRISRTVAVSCXXXXXXXX 239 AA L WH G+ GL +R + P+ + ISR +A++C Sbjct: 172 AAAITLLWHSAWSQVTVMILAGIAGLLFFRQSKVSDFPEFRVPISRALAIACWIVFFGLL 231 Query: 240 XXXXXXRTSAGAGEFALFDSFYRSGSLVFGGGXXXXXXXXXXXXXAGWVSREDFLAGYGA 299 R S G ALF+SFYR+GSLVFGGG GWV++E FL GYGA Sbjct: 232 LVLPILRESTGFSLLALFESFYRAGSLVFGGGHVVLPLLEREVIPTGWVTQEAFLTGYGA 291 Query: 300 AQAVPGPLFTFASYLGMMAAGFKGGIIATLGIFLPAFLLVIGALPFWNGLRSSPNVQGAL 359 QAVPGPLFTFA+YLG + AG G I+ATL IFLPAFLLVIGALPFWN LR + +QG L Sbjct: 292 TQAVPGPLFTFAAYLGALIAGGVGAIVATLAIFLPAFLLVIGALPFWNSLRRNSKIQGML 351 Query: 360 AGINAAVVGILLAALYDPLWTSAILKPLDFALAAILFVMLFFWKLPPWVIVLAGAAGGML 419 G+NAAVVGILLAALYDP+WTS IL P+DF +AAI+F+ML WKLPPW++V+ GA G+L Sbjct: 352 IGVNAAVVGILLAALYDPIWTSTILAPMDFIVAAIMFIMLEHWKLPPWLVVVTGALYGLL 411 >Q62NX4.1 Q62NX4_BACLD Chromate transporter Length = 397 Score = 428 bits (1101), Expect = e-118, Method: Compositional matrix adjust. Identities = 221/385 (57%), Positives = 270/385 (70%), Gaps = 1/385 (0%) Query: 40 LLEVLGVSLKLGLTSFGGPIAHLGYFHNEYIRRRKWMDERSYADLVALCQFLPGPASSQV 99 LLE+L VS +LGLTSFGGPIAHLGYFH EY+R+RKWMDE++YADLVALCQFLPGPASSQV Sbjct: 13 LLEILFVSTRLGLTSFGGPIAHLGYFHAEYVRKRKWMDEKNYADLVALCQFLPGPASSQV 72 Query: 100 GIGIGVVRAGLLGGFIAWLGFTLPSVIALAVFALVLQGVDIAGAGWIHGLKXXXXXXXXX 159 GIGIGV+RAG+LGG A++GFTLPSVIAL +FA+VLQG DI AGWIHGLK Sbjct: 73 GIGIGVMRAGVLGGISAFIGFTLPSVIALVLFAMVLQGFDIGTAGWIHGLKIVAVAVVAH 132 Query: 160 XXLGMGQKLTPDRGRVTIAVIAAVFALSWHXXXXXXXXXXXXGVIGLWLYRGQPDRGPD- 218 +GM QKLTPD+ R IA+ A V L W G +Y+ Q + Sbjct: 133 AVMGMAQKLTPDKRRKAIALFALVATLLWQTAFTQVGVILLAAFAGYLIYKEQVETDDSV 192 Query: 219 LPIRISRTVAVSCXXXXXXXXXXXXXXRTSAGAGEFALFDSFYRSGSLVFGGGXXXXXXX 278 + +SR A C R +G A+FDSFYRSGSLVFGGG Sbjct: 193 MQFPVSRRFAAVCLSLFFGLLILLPVLREMTSSGWIAMFDSFYRSGSLVFGGGHVVLPLL 252 Query: 279 XXXXXXAGWVSREDFLAGYGAAQAVPGPLFTFASYLGMMAAGFKGGIIATLGIFLPAFLL 338 AGW+S E FLAGYGAAQAVPGPLFTFA+YLG + +G++GG++AT IFLPAFLL Sbjct: 253 EREFVPAGWLSEEAFLAGYGAAQAVPGPLFTFAAYLGAVISGWQGGLLATAAIFLPAFLL 312 Query: 339 VIGALPFWNGLRSSPNVQGALAGINAAVVGILLAALYDPLWTSAILKPLDFALAAILFVM 398 ++G LPFW+ LR +P V+GAL G+NAAVVGIL++A Y P+WTS++L P+DFA AA+LF + Sbjct: 313 ILGTLPFWDTLRRNPKVKGALMGVNAAVVGILISAFYHPIWTSSVLAPIDFAFAAVLFSL 372 Query: 399 LFFWKLPPWVIVLAGAAGGMLIQLI 423 L +WKL PW+IV AGA GG ++QL+ Sbjct: 373 LVYWKLSPWIIVAAGAVGGAMLQLL 397 >Q65DF4.1 Q65DF4_BACLD Putative uncharacterized protein Length = 398 Score = 428 bits (1101), Expect = e-118, Method: Compositional matrix adjust. Identities = 221/385 (57%), Positives = 270/385 (70%), Gaps = 1/385 (0%) Query: 40 LLEVLGVSLKLGLTSFGGPIAHLGYFHNEYIRRRKWMDERSYADLVALCQFLPGPASSQV 99 LLE+L VS +LGLTSFGGPIAHLGYFH EY+R+RKWMDE++YADLVALCQFLPGPASSQV Sbjct: 14 LLEILFVSTRLGLTSFGGPIAHLGYFHAEYVRKRKWMDEKNYADLVALCQFLPGPASSQV 73 Query: 100 GIGIGVVRAGLLGGFIAWLGFTLPSVIALAVFALVLQGVDIAGAGWIHGLKXXXXXXXXX 159 GIGIGV+RAG+LGG A++GFTLPSVIAL +FA+VLQG DI AGWIHGLK Sbjct: 74 GIGIGVMRAGVLGGISAFIGFTLPSVIALVLFAMVLQGFDIGTAGWIHGLKIVAVAVVAH 133 Query: 160 XXLGMGQKLTPDRGRVTIAVIAAVFALSWHXXXXXXXXXXXXGVIGLWLYRGQPDRGPD- 218 +GM QKLTPD+ R IA+ A V L W G +Y+ Q + Sbjct: 134 AVMGMAQKLTPDKRRKAIALFALVATLLWQTAFTQVGVILLAAFAGYLIYKEQVETDDSV 193 Query: 219 LPIRISRTVAVSCXXXXXXXXXXXXXXRTSAGAGEFALFDSFYRSGSLVFGGGXXXXXXX 278 + +SR A C R +G A+FDSFYRSGSLVFGGG Sbjct: 194 MQFPVSRRFAAVCLSLFFGLLILLPVLREMTSSGWIAMFDSFYRSGSLVFGGGHVVLPLL 253 Query: 279 XXXXXXAGWVSREDFLAGYGAAQAVPGPLFTFASYLGMMAAGFKGGIIATLGIFLPAFLL 338 AGW+S E FLAGYGAAQAVPGPLFTFA+YLG + +G++GG++AT IFLPAFLL Sbjct: 254 EREFVPAGWLSEEAFLAGYGAAQAVPGPLFTFAAYLGAVISGWQGGLLATAAIFLPAFLL 313 Query: 339 VIGALPFWNGLRSSPNVQGALAGINAAVVGILLAALYDPLWTSAILKPLDFALAAILFVM 398 ++G LPFW+ LR +P V+GAL G+NAAVVGIL++A Y P+WTS++L P+DFA AA+LF + Sbjct: 314 ILGTLPFWDTLRRNPKVKGALMGVNAAVVGILISAFYHPIWTSSVLAPIDFAFAAVLFSL 373 Query: 399 LFFWKLPPWVIVLAGAAGGMLIQLI 423 L +WKL PW+IV AGA GG ++QL+ Sbjct: 374 LVYWKLSPWIIVAAGAVGGAMLQLL 398 >Q6HIU6.1 Q6HIU6_BACHK Chromate transporter Length = 397 Score = 427 bits (1099), Expect = e-118, Method: Compositional matrix adjust. Identities = 219/385 (56%), Positives = 268/385 (69%), Gaps = 1/385 (0%) Query: 40 LLEVLGVSLKLGLTSFGGPIAHLGYFHNEYIRRRKWMDERSYADLVALCQFLPGPASSQV 99 LLEVL VS +LGLTSFGGPIAHLGYFH EY+R+RKWMDE+SYADLVALCQFLPGPASSQV Sbjct: 13 LLEVLLVSTRLGLTSFGGPIAHLGYFHEEYVRKRKWMDEKSYADLVALCQFLPGPASSQV 72 Query: 100 GIGIGVVRAGLLGGFIAWLGFTLPSVIALAVFALVLQGVDIAGAGWIHGLKXXXXXXXXX 159 GIGIGV+RAG+LGG +A++GFTLPSVIAL +FALVLQG++I WIHGLK Sbjct: 73 GIGIGVMRAGVLGGILAFIGFTLPSVIALIIFALVLQGLNIGDVRWIHGLKIVAVVVVAH 132 Query: 160 XXLGMGQKLTPDRGRVTIAVIAAVFALSWHXXXXXXXXXXXXGVIGLWLYRGQPDRGP-D 218 LGM QKLTPD R +IA+ A V L W G++G +Y+ D Sbjct: 133 AILGMAQKLTPDLQRKSIALFALVGTLLWQTAFTQVGVILLSGLVGYLIYKNHADNDEAK 192 Query: 219 LPIRISRTVAVSCXXXXXXXXXXXXXXRTSAGAGEFALFDSFYRSGSLVFGGGXXXXXXX 278 L +ISR + V C R A+FDSFYR+GSLVFGGG Sbjct: 193 LSFQISRKLGVICLSLFFGLLIFLPILREVTSLKWIAMFDSFYRAGSLVFGGGHVVLPLL 252 Query: 279 XXXXXXAGWVSREDFLAGYGAAQAVPGPLFTFASYLGMMAAGFKGGIIATLGIFLPAFLL 338 GW+ FL+GYGA QAVPGPLFTFA+Y+G + ++GG++AT+ IFLPAFLL Sbjct: 253 EREFVPTGWMDEASFLSGYGATQAVPGPLFTFAAYMGAVIGEWQGGLLATVAIFLPAFLL 312 Query: 339 VIGALPFWNGLRSSPNVQGALAGINAAVVGILLAALYDPLWTSAILKPLDFALAAILFVM 398 ++G LPFW+ LR +P ++GAL G+NAAVVGIL++A Y P+WTSAIL+P+DFA AAILF M Sbjct: 313 ILGTLPFWDQLRRNPKIKGALMGVNAAVVGILISAFYHPIWTSAILQPVDFAFAAILFSM 372 Query: 399 LFFWKLPPWVIVLAGAAGGMLIQLI 423 L +WKLPPW+IV+ GA GG +I L+ Sbjct: 373 LVYWKLPPWIIVVVGAVGGTIISLL 397 >Q8ELF3.1 Q8ELF3_OCEIH Chromate transporter Length = 400 Score = 427 bits (1098), Expect = e-117, Method: Compositional matrix adjust. Identities = 218/387 (56%), Positives = 264/387 (68%), Gaps = 3/387 (0%) Query: 40 LLEVLGVSLKLGLTSFGGPIAHLGYFHNEYIRRRKWMDERSYADLVALCQFLPGPASSQV 99 LLE+L +S +LGLTSFGGPIAHLGYFH EY+RRRKWMDE+SYADLVAL QFLPGPASSQV Sbjct: 14 LLEILFISTRLGLTSFGGPIAHLGYFHEEYVRRRKWMDEKSYADLVALAQFLPGPASSQV 73 Query: 100 GIGIGVVRAGLLGGFIAWLGFTLPSVIALAVFALVLQGVDIAGAGWIHGLKXXXXXXXXX 159 GIGIGV+RAG LGG ++++GFTLPSVIAL +FAL++Q ++A GWIHGLK Sbjct: 74 GIGIGVMRAGTLGGIVSFIGFTLPSVIALVIFALLMQSFNVAEMGWIHGLKLVAVAIVAH 133 Query: 160 XXLGMGQKLTPDRGRVTIAVIAAVFALSWHXXXXXXXXXXXXGVIGLWLYRGQPDRGPDL 219 +GM +KLTPD R TIA++A V L W +IG LY+ + Sbjct: 134 AIIGMAKKLTPDVKRKTIALVALVITLLWQTVYSQVVVIFIAAMIGYVLYKNYKSDVEES 193 Query: 220 PIRI---SRTVAVSCXXXXXXXXXXXXXXRTSAGAGEFALFDSFYRSGSLVFGGGXXXXX 276 + ++ C R + A+FDSFYRSGSLVFGGG Sbjct: 194 HTHVKILTKKFGFICLSLFFGLLVLFPVLREITSSHWVAMFDSFYRSGSLVFGGGHVVLP 253 Query: 277 XXXXXXXXAGWVSREDFLAGYGAAQAVPGPLFTFASYLGMMAAGFKGGIIATLGIFLPAF 336 GWVS E FLAGYGAAQAVPGPLFTFA+YLG + G+ GG++AT+ IFLPAF Sbjct: 254 LLEREFVPTGWVSEEAFLAGYGAAQAVPGPLFTFAAYLGAVMNGWVGGLLATIAIFLPAF 313 Query: 337 LLVIGALPFWNGLRSSPNVQGALAGINAAVVGILLAALYDPLWTSAILKPLDFALAAILF 396 LL++G LPFWN LR++ ++GAL G+NAAVVGIL+AA Y P+WTSA+L P+DFA AA+LF Sbjct: 314 LLILGTLPFWNSLRNNHKIKGALMGVNAAVVGILIAAFYQPIWTSAVLSPVDFAFAAVLF 373 Query: 397 VMLFFWKLPPWVIVLAGAAGGMLIQLI 423 ML +WKLPPWVIVL GA GG LI I Sbjct: 374 SMLVYWKLPPWVIVLTGAIGGTLISYI 400 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.144 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,287,859,998 Number of extensions: 90742419 Number of successful extensions: 332190 Number of sequences better than 1.0e-05: 1053 Number of HSP's gapped: 330486 Number of HSP's successfully gapped: 1563 Length of query: 423 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 285 Effective length of database: 1,760,596,133 Effective search space: 501769897905 Effective search space used: 501769897905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)