BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0002.p (281 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I8D1.1 B8I8D1_CLOCE NUDIX hydrolase 296 2e-78 Q468G3.1 Q468G3_METBF Phosphohydrolase 271 6e-71 Q8PU15.1 Q8PU15_METMA Phosphohydrolase 270 1e-70 Q8TQV0.1 Q8TQV0_METAC NAD+ pyrophosphatase 266 2e-69 Q5P615.1 Q5P615_AZOSE NTP pyrophosphohydrolases containing a Zn-... 259 1e-67 >B8I8D1.1 B8I8D1_CLOCE NUDIX hydrolase Length = 280 Score = 296 bits (757), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 138/268 (51%), Positives = 184/268 (68%) Query: 7 SIYKRYIPAVTPPLEFSGSAYWLIFHSGRLLVTENAGITGIPFIPALEQISMTPLRTLYL 66 +IYK Y PAVT P + YW IF S +LL+ N IP L ++S LYL Sbjct: 5 NIYKNYEPAVTAPDKDYKCGYWFIFCSNKLLIVNNENEAKIPQYNELSELSQVLTSPLYL 64 Query: 67 GTFEGIACFAAQVQAETPEPEGMAFRPLRSLYESLDEDLFHLAGKGIQMLAWDETHQFCG 126 G F G+ + + T P GM+F LRS + LDE F LA K +Q++AW++TH+FCG Sbjct: 65 GRFYGLPAYCIGLADSTQAPSGMSFVDLRSTFGVLDEASFLLASKAVQVVAWEQTHRFCG 124 Query: 127 RCGTATELVQTEHSRSCPACGLVSYPRIAPAVITAILKDNRILLAHSPHFVNNMYGLIAG 186 +CG+ T+ + E ++ CP CG +SYPRI PAVITA+ K+N+ILLAH+ F +++ LIAG Sbjct: 125 KCGSLTQDLSGERAKVCPKCGFISYPRICPAVITAVFKENKILLAHARSFKGDIHSLIAG 184 Query: 187 FVEPGETLEDCVQRETMEEVGIKVKNIRYFASQQWPFPHSLMVGFLAEYESGEITVDGEE 246 FVE GETLE+ V+RE MEE+ IKVKNI+Y+ SQ WP+P+SLM+GF AEYE GEI VDG E Sbjct: 185 FVEAGETLEEAVEREIMEEISIKVKNIKYWGSQPWPYPNSLMLGFTAEYEGGEINVDGVE 244 Query: 247 LDHADWFELDRLPVIPSPVSIARKLIDW 274 + HA W++++ LP +P VSIARK+IDW Sbjct: 245 ISHAHWYDVENLPELPPKVSIARKIIDW 272 >Q468G3.1 Q468G3_METBF Phosphohydrolase Length = 289 Score = 271 bits (692), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 127/269 (47%), Positives = 173/269 (64%) Query: 9 YKRYIPAVTPPLEFSGSAYWLIFHSGRLLVTENAGITGIPFIPALEQISMTPLRTLYLGT 68 + +I + PP + A W IF +L+ N IP + ++ ++ +R YLGT Sbjct: 15 FPEFIVGIEPPANRTEKALWFIFRGREVLLKLNKNPGAIPKMLDFGELGLSEIREQYLGT 74 Query: 69 FEGIACFAAQVQAETPEPEGMAFRPLRSLYESLDEDLFHLAGKGIQMLAWDETHQFCGRC 128 EG C++ ++ + PEGM F LR Y ++E F L K +Q++ WD T+Q+C RC Sbjct: 75 LEGTHCYSVELPEDAQAPEGMKFADLRQAYTEMNEKCFALVNKAVQIMEWDRTNQYCSRC 134 Query: 129 GTATELVQTEHSRSCPACGLVSYPRIAPAVITAILKDNRILLAHSPHFVNNMYGLIAGFV 188 G T E + CP CG + YPRI+PAVI I K++ ILLA SP+F+ MYGLIAGFV Sbjct: 135 GAKTLQKPGERGKECPECGELFYPRISPAVIVLIRKEHEILLARSPNFMPGMYGLIAGFV 194 Query: 189 EPGETLEDCVQRETMEEVGIKVKNIRYFASQQWPFPHSLMVGFLAEYESGEITVDGEELD 248 EPGE+ E V RET EEVGIKVKNI YF +Q WPFP+SLM+GF AEY+SGEI DG E++ Sbjct: 195 EPGESAETAVVRETREEVGIKVKNISYFGTQAWPFPNSLMIGFTAEYDSGEIQPDGFEIE 254 Query: 249 HADWFELDRLPVIPSPVSIARKLIDWVVE 277 A WF +++LP +P +SIARKLID+ ++ Sbjct: 255 DAGWFSVEKLPGLPGKISIARKLIDYFLK 283 >Q8PU15.1 Q8PU15_METMA Phosphohydrolase Length = 285 Score = 270 bits (689), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 126/266 (47%), Positives = 170/266 (63%) Query: 8 IYKRYIPAVTPPLEFSGSAYWLIFHSGRLLVTENAGITGIPFIPALEQISMTPLRTLYLG 67 I+ ++I + PP + A W IF +L+ IP + + ++ + +R YLG Sbjct: 10 IHPKFIVGIEPPAGRTKKALWFIFRGRDILIKTYKNPGAIPRLLDIRELGLPVIREQYLG 69 Query: 68 TFEGIACFAAQVQAETPEPEGMAFRPLRSLYESLDEDLFHLAGKGIQMLAWDETHQFCGR 127 EG CF+ ++ + PEGM F LR Y + E F L K +Q++ WD T+QFC R Sbjct: 70 VLEGTDCFSIELPQDAKAPEGMEFVDLRQAYSEMSEGCFELVNKAVQVMEWDRTNQFCSR 129 Query: 128 CGTATELVQTEHSRSCPACGLVSYPRIAPAVITAILKDNRILLAHSPHFVNNMYGLIAGF 187 CGT T E + CP CG + YPRI+PAVI I K ++ILLA SP+F +MY LIAGF Sbjct: 130 CGTKTSQKPGERGKECPECGELFYPRISPAVIVLIRKGSKILLARSPNFPPDMYSLIAGF 189 Query: 188 VEPGETLEDCVQRETMEEVGIKVKNIRYFASQQWPFPHSLMVGFLAEYESGEITVDGEEL 247 VEPGE+ E V+RE EEVGIKVKN+ YF +Q WPFP+SLM+GF AEY+SGEI DG E+ Sbjct: 190 VEPGESAEAAVEREIWEEVGIKVKNVTYFGTQAWPFPNSLMIGFTAEYDSGEIRPDGFEI 249 Query: 248 DHADWFELDRLPVIPSPVSIARKLID 273 + A WF +++LPV+P +SI+RKLID Sbjct: 250 EDAKWFSVNKLPVLPGKISISRKLID 275 >Q8TQV0.1 Q8TQV0_METAC NAD+ pyrophosphatase Length = 285 Score = 266 bits (679), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 124/265 (46%), Positives = 168/265 (63%) Query: 9 YKRYIPAVTPPLEFSGSAYWLIFHSGRLLVTENAGITGIPFIPALEQISMTPLRTLYLGT 68 Y ++I + PP + A W IF +L+ IP + E++ + +R YLG Sbjct: 11 YPKFIVGIEPPAGRAEKALWFIFRGRNILIKVRKNPGAIPKLVDFEELGLPAIREQYLGM 70 Query: 69 FEGIACFAAQVQAETPEPEGMAFRPLRSLYESLDEDLFHLAGKGIQMLAWDETHQFCGRC 128 EG C++ ++ A+T PEGM F LR + + E F L K +Q++ WD T+QFC RC Sbjct: 71 LEGTHCYSVELPADTKAPEGMEFVDLRKAHSEISEGCFELVNKAVQVMEWDRTNQFCSRC 130 Query: 129 GTATELVQTEHSRSCPACGLVSYPRIAPAVITAILKDNRILLAHSPHFVNNMYGLIAGFV 188 GT T E + CPACG + YPRI+PA+I I K + +LLA SP+F ++Y LIAGFV Sbjct: 131 GTKTVQKPGERGKECPACGELFYPRISPAIIVLIRKGHEVLLARSPNFPPDVYSLIAGFV 190 Query: 189 EPGETLEDCVQRETMEEVGIKVKNIRYFASQQWPFPHSLMVGFLAEYESGEITVDGEELD 248 EPGET E V RE EEVG+KVKNI YF +Q WPFP+SLM+GF AEY+SG+I DG E++ Sbjct: 191 EPGETAEAAVSREVWEEVGLKVKNITYFGTQAWPFPNSLMIGFTAEYDSGDIRPDGFEIE 250 Query: 249 HADWFELDRLPVIPSPVSIARKLID 273 A WF ++ LP +P +SI+RKLID Sbjct: 251 DAKWFSVEELPALPGKISISRKLID 275 >Q5P615.1 Q5P615_AZOSE NTP pyrophosphohydrolases containing a Zn-finger, probably nucleic-acid-binding Length = 279 Score = 259 bits (663), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 124/268 (46%), Positives = 172/268 (64%), Gaps = 1/268 (0%) Query: 12 YIPAVTPPLEFSGSAYWLIFHSGRLLVTENAGITGIPFIPALEQISMTPLRTL-YLGTFE 70 ++P +T P + S S W +F++ RLLV +P LE++ + R YLG Sbjct: 6 FVPGITEPSQASDSDLWFVFNADRLLVIGEGESARVPSRGELEELGVAAGRCRHYLGRLG 65 Query: 71 GIACFAAQVQAETPEPEGMAFRPLRSLYESLDEDLFHLAGKGIQMLAWDETHQFCGRCGT 130 CF A +A PE ++ LR L+ +DE + +AG+ +Q++ WD+TH++CGRCG+ Sbjct: 66 ERDCFTAAFEASPELPERAEWQGLRRLFGRIDETVLAIAGRAVQIIEWDDTHRYCGRCGS 125 Query: 131 ATELVQTEHSRSCPACGLVSYPRIAPAVITAILKDNRILLAHSPHFVNNMYGLIAGFVEP 190 AT L Q E +R CP CGL YP ++PAV+ + + +LLA SPHF MY +AGFVEP Sbjct: 126 ATGLKQGERARVCPDCGLAGYPCLSPAVMGLVRRGRELLLARSPHFPEGMYSALAGFVEP 185 Query: 191 GETLEDCVQRETMEEVGIKVKNIRYFASQQWPFPHSLMVGFLAEYESGEITVDGEELDHA 250 GETLE +QRE EEVG+++ N+RYF SQ WPFPHSLM+ F+A+Y SGEI E++ A Sbjct: 186 GETLEQTLQREVREEVGVEITNLRYFDSQPWPFPHSLMIAFVADYVSGEIVPQPGEIEAA 245 Query: 251 DWFELDRLPVIPSPVSIARKLIDWVVEN 278 DWF +DRLP +P P SIAR+LID V + Sbjct: 246 DWFGIDRLPRLPHPFSIARRLIDETVAS 273 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,722,582,113 Number of extensions: 70085006 Number of successful extensions: 172440 Number of sequences better than 1.0e-05: 924 Number of HSP's gapped: 172535 Number of HSP's successfully gapped: 924 Length of query: 281 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 147 Effective length of database: 1,798,280,193 Effective search space: 264347188371 Effective search space used: 264347188371 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)