BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0027.p (96 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65KE5.1 Q65KE5_BACLD Putative uncharacterized protein 56 1e-06 Q65CR4.1 Q65CR4_BACLD Putative uncharacterized protein 53 9e-06 >Q65KE5.1 Q65KE5_BACLD Putative uncharacterized protein Length = 85 Score = 55.8 bits (133), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 34/86 (39%), Positives = 41/86 (47%), Gaps = 1/86 (1%) Query: 10 LDNVLAFASVLAVFTLALVQLIKNNINLPRNAVPFXXXXXXXXXXXXXYPFTDLGLTLRX 69 ++ VL FA+VLA ALVQL+K IN+P N VP YPFTDL L LR Sbjct: 1 MEEVLLFATVLAPILTALVQLVKKTINMPTNVVPAVSFVLGIALGAVAYPFTDLELVLRL 60 Query: 70 XXXXXXXXXXXXXFELAFNDRPGTTQ 95 FE+ R GTT+ Sbjct: 61 WAGGFAGLAATGLFEIG-AKREGTTK 85 >Q65CR4.1 Q65CR4_BACLD Putative uncharacterized protein Length = 88 Score = 53.1 bits (126), Expect = 9e-06, Method: Compositional matrix adjust. Identities = 28/79 (35%), Positives = 38/79 (48%) Query: 10 LDNVLAFASVLAVFTLALVQLIKNNINLPRNAVPFXXXXXXXXXXXXXYPFTDLGLTLRX 69 ++ VL FASVL+ LALV+L+K + +P+N +P YPFTDL L LR Sbjct: 1 METVLIFASVLSPIILALVELVKKTVKMPKNLIPLVSLLIGLLIGAAAYPFTDLELVLRL 60 Query: 70 XXXXXXXXXXXXXFELAFN 88 FE+ N Sbjct: 61 WSGGLAGLTATGLFEIGKN 79 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.144 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 339,996,018 Number of extensions: 7132527 Number of successful extensions: 14762 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 14761 Number of HSP's successfully gapped: 2 Length of query: 96 Length of database: 3,060,696,203 Length adjustment: 65 Effective length of query: 31 Effective length of database: 2,448,330,228 Effective search space: 75898237068 Effective search space used: 75898237068 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)