BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0015.p (354 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D682.1 B1D682_9BACL Baseplate J family protein 324 8e-87 B3KEC5.1 B3KEC5_9BACI Baseplate J family protein 303 2e-80 Q24QF4.1 Q24QF4_DESHY Putative uncharacterized protein 276 3e-72 B8FNV2.1 B8FNV2_DESHD Baseplate J family protein 276 3e-72 Q65L14.1 Q65L14_BACLD YqbT 265 7e-69 >B1D682.1 B1D682_9BACL Baseplate J family protein Length = 353 Score = 324 bits (831), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 168/353 (47%), Positives = 225/353 (63%), Gaps = 1/353 (0%) Query: 3 VYEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTA 62 +YE QTFE +L RMLDRVP ++DKREGSIIYD Q+Y +LD+N NL F DT+ Sbjct: 1 MYETQTFETILQRMLDRVPGAIDKREGSIIYDALAPAAAELTQIYADLDINLNLVFGDTS 60 Query: 63 TGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPG 122 +GEYL R A GI R+ ASKA+ +FYN A+ L+DVP+GSR+SLD L + EK++ G Sbjct: 61 SGEYLARRTADFGIERQLASKAQRLGLFYNGANVLMDVPIGSRYSLDGLSFKVTEKISTG 120 Query: 123 TYRLESETAGEEGNRYFGSLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESVRR 182 Y+L+SETAG GN +G +LPIDYI LA+ ++ +LIPGEN EDD +LRQR+L V++ Sbjct: 121 NYKLQSETAGSAGNANYGQMLPIDYINGLAKAVLSEVLIPGENEEDDHSLRQRFLAKVQK 180 Query: 183 PSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQYID 242 P TSGN Y WAL + GVG A V PLWNGP TVKV I+D+ RPAS L +VQ +I Sbjct: 181 PGTSGNVSDYKSWALTVSGVGAAYVKPLWNGPGTVKVTILDSEMRPASAALTGEVQHFIS 240 Query: 243 PVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEILEKYRKEKAF 302 P+ G G+ G +++++AK+ L SL +V + + Y K AF Sbjct: 241 PLPGMGQEVAPIGAIVTVAPAEGVAVNLTAKLLLNGSASLADVQKNVESAVAAYLKGLAF 300 Query: 303 TASYISQSV-IGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSVVLEV 354 + V IG+LLLD +GV DYS L++NG N+ +S +V + +V LEV Sbjct: 301 AEDSSPKYVRIGALLLDIDGVNDYSFLRINGGISNIPISPDQVAILGTVDLEV 353 >B3KEC5.1 B3KEC5_9BACI Baseplate J family protein Length = 352 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 153/349 (43%), Positives = 221/349 (63%), Gaps = 1/349 (0%) Query: 5 EDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTATG 64 E+QT+EA+L RML+R+P +DKREGSIIYD QMY+EL+ + NL FA TA+G Sbjct: 3 EEQTYEAILERMLERIPEGMDKREGSIIYDALAPSAAELAQMYVELEYSMNLKFAATASG 62 Query: 65 EYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPGTY 124 E+L+RSIAWSG+TR+ A+KA+L F S +DVP+GSRFSLD L+Y + +L G Y Sbjct: 63 EFLDRSIAWSGLTRKQATKAQLLGQFSGSNGAPVDVPIGSRFSLDPLNYKVMTRLGAGQY 122 Query: 125 RLESETAGEEGNRYFGSLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESVRRPS 184 LE ET+G+EGNR FGSLLP++Y+ L + E+ L +PGE+ E DEAL RY E + RP Sbjct: 123 VLECETSGKEGNRRFGSLLPLEYVEGLVKAELVELWVPGEDTESDEALYDRYREKISRPV 182 Query: 185 TSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQYIDP- 243 TS N+ Y WA + GVG A+ FPLW+GP TVKVV++D R + +V VQQ+IDP Sbjct: 183 TSANRNQYELWAREKAGVGKAKAFPLWDGPGTVKVVLLDNEMRSPTPSVVESVQQHIDPT 242 Query: 244 VSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEILEKYRKEKAFT 303 + G GEG +++ +VTL +G + V +A ++ + +Y K+ A + Sbjct: 243 MDGMGEGAAPVGSVVTVVGAAEVPVNIEVQVTLLNGAGIDGVQEAIEQGVRQYLKDLAMS 302 Query: 304 ASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSVVL 352 + + I +++LDT V+DY L +NG T ++ + + V + +V + Sbjct: 303 DPLVRYNRIANVILDTPAVIDYEVLTVNGGTDSIPIQQEAVAVLGTVTV 351 >Q24QF4.1 Q24QF4_DESHY Putative uncharacterized protein Length = 359 Score = 276 bits (705), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 153/359 (42%), Positives = 214/359 (59%), Gaps = 11/359 (3%) Query: 3 VYEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTA 62 +YE QT+EA+L RML RVP ++DKREGS+IYD Q Y EL VN+NL FADTA Sbjct: 1 MYEHQTYEAILVRMLARVPETIDKREGSLIYDALAPGAAELAQAYAELVVNNNLSFADTA 60 Query: 63 TGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPG 122 +GE+L R A GI R+ A+KA + F +S + LL++PLGSR+S+ L+YTA+E+++ G Sbjct: 61 SGEFLTRRAAEFGIKRKVATKAWRRGGFLDSNNALLNIPLGSRYSIGGLNYTAIEQISTG 120 Query: 123 TYRLESETAGEEGNRYFGSLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESVRR 182 Y LE ETAG GN+ FG LLPI Y+ LAR E+T +L+PGE+ E DEAL RY E+V Sbjct: 121 AYVLECETAGTAGNQQFGDLLPIAYVNSLARAELTEVLVPGEDEETDEALLSRYYETVNE 180 Query: 183 PSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQYID 242 P+ GN Y + I+GVGG +VFP W G TVK I+ + S LV++VQ +D Sbjct: 181 PAFGGNMADYRQKVNAINGVGGTKVFPTWAGGGTVKCTIIASDWAKPSPELVNEVQSTMD 240 Query: 243 PVSGQGE--GQXXXXXXXXXXXXXGKSISVSAKVTLASGYS-------LQEVIDAFKEIL 293 P QG GQ ++ V +TLA G + ++ ++DA+ +L Sbjct: 241 PEENQGMGLGQAPIGHAVTIAGVDKVTVDVETTLTLAPGVTPGQVQSDVEAILDAY--LL 298 Query: 294 EKYRKEKAFTASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSVVL 352 E+ + A T I + I + +L +GV D +G KLNG N L E E+P+ +V++ Sbjct: 299 EQRKTWAAQTQIIIRMAQIDARMLTVQGVEDVTGTKLNGTEANFLLDEEEIPILGTVII 357 >B8FNV2.1 B8FNV2_DESHD Baseplate J family protein Length = 359 Score = 276 bits (705), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 153/359 (42%), Positives = 214/359 (59%), Gaps = 11/359 (3%) Query: 3 VYEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTA 62 +YE QT+EA+L RML RVP ++DKREGS+IYD Q Y EL VN+NL FADTA Sbjct: 1 MYEHQTYEAILVRMLARVPETIDKREGSLIYDALAPGAAELAQAYAELVVNNNLSFADTA 60 Query: 63 TGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPG 122 +GE+L R A GI R+ A+KA + F +S + LL++PLGSR+S+ L+YTA+E+++ G Sbjct: 61 SGEFLTRRAAEFGIKRKVATKAWRRGGFLDSNNALLNIPLGSRYSIGGLNYTAIEQISTG 120 Query: 123 TYRLESETAGEEGNRYFGSLLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESVRR 182 Y LE ETAG GN+ FG LLPI Y+ LAR E+T +L+PGE+ E DEAL RY E+V Sbjct: 121 AYVLECETAGTAGNQQFGDLLPIAYVNSLARAELTEVLVPGEDEETDEALLSRYYETVNE 180 Query: 183 PSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQYID 242 P+ GN Y + I+GVGG +VFP W G TVK I+ + S LV++VQ +D Sbjct: 181 PAFGGNMADYRQKVNAINGVGGTKVFPTWAGGGTVKCTIIASDWAKPSPELVNEVQSTMD 240 Query: 243 PVSGQGE--GQXXXXXXXXXXXXXGKSISVSAKVTLASGYS-------LQEVIDAFKEIL 293 P QG GQ ++ V +TLA G + ++ ++DA+ +L Sbjct: 241 PEENQGMGLGQAPIGHAVTIAGVDKVTVDVETTLTLAPGVTPGQVQSDVEAILDAY--LL 298 Query: 294 EKYRKEKAFTASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSVVL 352 E+ + A T I + I + +L +GV D +G KLNG N L E E+P+ +V++ Sbjct: 299 EQRKTWAAQTQIIIRMAQIDARMLTVQGVEDVTGTKLNGTEANFLLDEEEIPILGTVII 357 >Q65L14.1 Q65L14_BACLD YqbT Length = 347 Score = 265 bits (676), Expect = 7e-69, Method: Compositional matrix adjust. Identities = 149/352 (42%), Positives = 208/352 (59%), Gaps = 11/352 (3%) Query: 3 VYEDQTFEALLGRMLDRVPSSLDKREGSIIYDXXXXXXXXXXQMYIELDVNSNLFFADTA 62 ++EDQTFE ++ RML+RV +DKRE S+I++ YI LD NL +ADTA Sbjct: 1 MFEDQTFEEIMERMLERVSDEIDKRENSVIWNALAPAAAELALSYIWLDQVLNLVYADTA 60 Query: 63 TGEYLERSIAWSGITRRAASKAELKAMFYNSASGLLDVPLGSRFSLDLLHYTAVEKLAPG 122 GEYL+R A +G+ R ASKA A F + S VP G+RF L+ L++T +E Sbjct: 61 EGEYLDRRAAEAGLERYRASKAIWSAAFTDGVS----VPPGTRFFLEDLYFTMLED---- 112 Query: 123 TYRLESETAGEEGNRYFGS--LLPIDYIPVLARGEITALLIPGENAEDDEALRQRYLESV 180 +LE ETAG +GN F LLP+D IP L + + +L IPG + E DE+L +RYL V Sbjct: 113 -GKLECETAGTKGNANFSGRPLLPLDTIPGLEKAVMGSLEIPGRDEETDESLYERYLIRV 171 Query: 181 RRPSTSGNKYHYMEWALQIDGVGGARVFPLWNGPKTVKVVIVDAAKRPASELLVSQVQQY 240 RR + S N+ HY +WA ++DGVG A+VFPLWNG TVKVVI +A PA++ L+ +V+QY Sbjct: 172 RREAVSANQLHYKQWAEEVDGVGKAKVFPLWNGEGTVKVVITNAKMEPATDALIERVKQY 231 Query: 241 IDPVSGQGEGQXXXXXXXXXXXXXGKSISVSAKVTLASGYSLQEVIDAFKEILEKYRKEK 300 IDP G+GEG K +S+SAK+ SG ++ E E + + KE Sbjct: 232 IDPDPGKGEGMAPIGAYTAVESAVWKDVSISAKIIPESGRTIGEAKQEIAEKITELFKEM 291 Query: 301 AFTASYISQSVIGSLLLDTEGVVDYSGLKLNGVTGNVTLSETEVPLFSSVVL 352 AF S I S I +++ ++ V DYS + +NG N+ L+ETE+P V + Sbjct: 292 AFKESVIRLSQINNMIYESSSVSDYSEVFINGEAKNLQLTETEIPKLGQVTI 343 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,878,749,549 Number of extensions: 72454224 Number of successful extensions: 131303 Number of sequences better than 1.0e-05: 114 Number of HSP's gapped: 131138 Number of HSP's successfully gapped: 115 Length of query: 354 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 217 Effective length of database: 1,770,017,148 Effective search space: 384093721116 Effective search space used: 384093721116 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)