BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0019.p (543 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DD70.1 B1DD70_9BACL AMP-dependent synthetase and ligase 749 0.0 B4BPR8.1 B4BPR8_9BACI AMP-dependent synthetase and ligase 659 0.0 C3IXL6.1 C3IXL6_9BACI AMP-dependent synthetase and ligase 657 0.0 B7YAL7.1 B7YAL7_9BACI AMP-dependent synthetase and ligase 657 0.0 A4IRT7.1 A4IRT7_GEOTN Acetyl-CoA synthetase 657 0.0 >B1DD70.1 B1DD70_9BACL AMP-dependent synthetase and ligase Length = 525 Score = 749 bits (1934), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/524 (67%), Positives = 427/524 (81%), Gaps = 4/524 (0%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLG 73 ++ QWIAPE YN++SE+E H +D++AL+ L + G+ ITY DL+ Q N+LAGGL+ LG Sbjct: 1 MNFDQWIAPEQYNISSEIERHKSDKLALRLLDDQGHTTHITYNDLLNQVNQLAGGLNELG 60 Query: 74 LTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSE 133 L +GDRVLVMVPR I AY+IY+ACLKLGL +IPSSEMLRAKDL+YRL+HSEARAVI W+E Sbjct: 61 LAKGDRVLVMVPRTIEAYMIYVACLKLGLVIIPSSEMLRAKDLSYRLQHSEARAVIAWAE 120 Query: 134 VTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILA 193 EV+ IT +LP L +R+ G + + GW L LMEGQPE A A+ RDD+AILA Sbjct: 121 GAAEVDNITEELPELQFRIDASGGQAD---GWITLGSLMEGQPEEREAEATHRDDMAILA 177 Query: 194 YTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLG 253 YTSGTTGNPK VVH+HGWGYAHL+I ++ WL I+ SD VWATAAPGWQKWIW+PFL+VLG Sbjct: 178 YTSGTTGNPKGVVHSHGWGYAHLRITAA-WLGIQSSDLVWATAAPGWQKWIWTPFLSVLG 236 Query: 254 NGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAG 313 +GATG +YNG F +R+L+++Q+ +QV+CCTPTEYR+MAKT+GL+ YDLS +R AVSAG Sbjct: 237 SGATGLIYNGSFSAERYLRIIQENQVQVMCCTPTEYRIMAKTDGLSGYDLSSIRSAVSAG 296 Query: 314 EPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGE 373 EPLNQ VI F E F IRDGYGQTE+TL+I LKD PI++GSMGKSI PG+VE+ID E Sbjct: 297 EPLNQAVIKQFEELFGFIIRDGYGQTENTLLIGNLKDSPIKIGSMGKSIVPGLVEIIDDE 356 Query: 374 GNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGR 433 G P P G VG IAVH MPALFK+YYKDP+RK + G YF+TGDRA KDE+GY WFEGR Sbjct: 357 GRPQPMGEVGSIAVHTDMPALFKSYYKDPDRKGDNIVGSYFITGDRASKDEEGYLWFEGR 416 Query: 434 GDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPEL 493 GDDIIISSGYTIGPFEVEEAL++H VKECA VASPDEIRG +VKAF+VL+D +G+PEL Sbjct: 417 GDDIIISSGYTIGPFEVEEALLRHPSVKECAAVASPDEIRGHIVKAFIVLRDGVSGSPEL 476 Query: 494 VKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQE 537 V+ELQ HVK TAPYKYPRKIEF+++LPKT SGKIRR+ELR +E Sbjct: 477 VQELQDHVKSVTAPYKYPRKIEFVSELPKTNSGKIRRIELRQRE 520 >B4BPR8.1 B4BPR8_9BACI AMP-dependent synthetase and ligase Length = 531 Score = 659 bits (1699), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/532 (61%), Positives = 387/532 (72%), Gaps = 9/532 (1%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPA---DRIALKWLQENGNYEEITYGDLMKQANRLAGGLS 70 + ++ IAPE YNLTSEME H A +RIALKW E G +EITYG LM +AN++ Sbjct: 1 MKREELIAPERYNLTSEMERHAAAHPERIALKWENERGETKEITYGRLMARANQIGNAFL 60 Query: 71 GLGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIV 130 L +GD+VLVMVPR I AY +YL LK GL VIPSSEMLR KDL YRL H E +A++ Sbjct: 61 SRRLEKGDKVLVMVPRLIEAYEVYLGALKAGLVVIPSSEMLRTKDLEYRLSHGEVKAIVA 120 Query: 131 WSEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIA 190 + E I G L + ++G E + GW L+ M + E+ A + RDD+A Sbjct: 121 YEPYLDEFAPIDGVDKLL--KFSIGEHEHD---GWVRLDAAMAAESETLAAADTSRDDMA 175 Query: 191 ILAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLT 250 L+YTSGTTGNPK VVH HGW YAHL+ + WL I E+D VWATA PGWQKWIWSPFL+ Sbjct: 176 FLSYTSGTTGNPKGVVHCHGWAYAHLRTAAKNWLCIEETDLVWATAGPGWQKWIWSPFLS 235 Query: 251 VLGNGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAV 310 VLG+GATGFVY G F P+++LQLL+ + VLCCTPTEYRLMAK + RYDLS L AV Sbjct: 236 VLGSGATGFVYYGRFEPEKYLQLLEKYQVNVLCCTPTEYRLMAKVPDIGRYDLSHLHSAV 295 Query: 311 SAGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVI 370 SAGEPLN+EVI+TF +HF I +RDGYGQTE+TL++ +K I+ GSMGK VE+I Sbjct: 296 SAGEPLNREVIDTFAKHFGIQVRDGYGQTENTLLVGVMKGMDIKPGSMGKPTPGNRVEII 355 Query: 371 DGEGNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWF 430 D G P P GVVGDIAVH+ PALFK YYKDPER G+Y++TGD+ARKDEDGYFWF Sbjct: 356 DENGEPCPVGVVGDIAVHVETPALFKYYYKDPERTAMQFRGDYYITGDKARKDEDGYFWF 415 Query: 431 EGRGDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDH-SAG 489 EGRGDDIIISSGYTIGPFEVE+AL+KH VKECAVVASPDE+RG VVKAFVVL+D Sbjct: 416 EGRGDDIIISSGYTIGPFEVEDALVKHPAVKECAVVASPDEVRGHVVKAFVVLRDGVDPN 475 Query: 490 TPELVKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAA 541 P L+ LQ HVK+ TAPYKYPRKIEF+ DLPKT SGKIRRVELR++E + A Sbjct: 476 DPSLIPALQEHVKQLTAPYKYPRKIEFVDDLPKTVSGKIRRVELREREARLA 527 >C3IXL6.1 C3IXL6_9BACI AMP-dependent synthetase and ligase Length = 530 Score = 657 bits (1696), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/532 (61%), Positives = 388/532 (72%), Gaps = 10/532 (1%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPA---DRIALKWLQENGNYEEITYGDLMKQANRLAGGLS 70 + + IAPE YNLTSEME H A DRIALKW E G +EITYG LM +AN++ Sbjct: 1 MRREDLIAPERYNLTSEMERHAAAGPDRIALKWESEQGETKEITYGRLMARANQIGNAFL 60 Query: 71 GLGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIV 130 GL +GD+VLVM+PR I Y +Y+ LK GL VIPSSEMLR KDL YRL HSEA+AV+ Sbjct: 61 SHGLEKGDKVLVMMPRLIETYEVYIGALKAGLVVIPSSEMLRTKDLQYRLVHSEAKAVVA 120 Query: 131 WSEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIA 190 ++ T E I G + L + +G E GW LE M + E+ A + RDD+A Sbjct: 121 YAPYTDEFAPIDG-IERLT-KFVIG----EPRDGWIPLEDAMAKESETLEAADTSRDDMA 174 Query: 191 ILAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLT 250 L+YTSGTTGNPK VVH HGW YAHL+ + WL I E D VWATA PGWQKWIWSPFL+ Sbjct: 175 FLSYTSGTTGNPKGVVHCHGWAYAHLRTAAKNWLCIEEGDLVWATAGPGWQKWIWSPFLS 234 Query: 251 VLGNGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAV 310 VLG+GATGFVY G F P+++LQLL+ I VLCCTPTEYRLMAK + RY LS L AV Sbjct: 235 VLGSGATGFVYYGRFEPEKYLQLLEKYNINVLCCTPTEYRLMAKVPDIGRYHLSHLHSAV 294 Query: 311 SAGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVI 370 SAGEPLN+EVI+TF +HF + +RDGYGQTE+TL++ +K PI+ GSMGK VE+I Sbjct: 295 SAGEPLNREVIDTFAKHFGVEVRDGYGQTENTLLVGVMKGMPIKPGSMGKPTPGNRVEII 354 Query: 371 DGEGNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWF 430 D G P P G VGDIAVH+ PALFK YYKDPER G+Y++TGD+ARKDEDGYFWF Sbjct: 355 DENGEPCPPGQVGDIAVHIETPALFKYYYKDPERTAMQFRGDYYITGDKARKDEDGYFWF 414 Query: 431 EGRGDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDH-SAG 489 EGRGDDIIIS+GYTIGPFEVE+AL+KH VKECAVVASPDE+RG VVKAFVVL+D Sbjct: 415 EGRGDDIIISAGYTIGPFEVEDALVKHPAVKECAVVASPDEVRGHVVKAFVVLRDGVDKD 474 Query: 490 TPELVKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAA 541 P L+ LQ HVK+ TAPYKYPRKIEF+ DLPKTASGKIRRVELR++E + A Sbjct: 475 DPSLIPALQEHVKQLTAPYKYPRKIEFVDDLPKTASGKIRRVELREREARLA 526 >B7YAL7.1 B7YAL7_9BACI AMP-dependent synthetase and ligase Length = 530 Score = 657 bits (1696), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/532 (61%), Positives = 388/532 (72%), Gaps = 10/532 (1%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPA---DRIALKWLQENGNYEEITYGDLMKQANRLAGGLS 70 + + IAPE YNLTSEME H A DRIALKW E G +EITYG LM +AN++ Sbjct: 1 MRREDLIAPERYNLTSEMERHAAAGPDRIALKWESEQGETKEITYGRLMARANQIGNAFL 60 Query: 71 GLGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIV 130 GL +GD+VLVM+PR I Y +Y+ LK GL VIPSSEMLR KDL YRL HSEA+AV+ Sbjct: 61 SHGLEKGDKVLVMMPRLIETYEVYIGALKAGLVVIPSSEMLRTKDLQYRLVHSEAKAVVA 120 Query: 131 WSEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIA 190 ++ T E I G + L + +G E GW LE M + E+ A + RDD+A Sbjct: 121 YAPYTDEFAPIDG-IERLT-KFVIG----EPRDGWIPLEDAMAKESETLEAADTSRDDMA 174 Query: 191 ILAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLT 250 L+YTSGTTGNPK VVH HGW YAHL+ + WL I E D VWATA PGWQKWIWSPFL+ Sbjct: 175 FLSYTSGTTGNPKGVVHCHGWAYAHLRTAAKNWLCIEEGDLVWATAGPGWQKWIWSPFLS 234 Query: 251 VLGNGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAV 310 VLG+GATGFVY G F P+++LQLL+ I VLCCTPTEYRLMAK + RY LS L AV Sbjct: 235 VLGSGATGFVYYGRFEPEKYLQLLEKYNINVLCCTPTEYRLMAKVPDIGRYHLSHLHSAV 294 Query: 311 SAGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVI 370 SAGEPLN+EVI+TF +HF + +RDGYGQTE+TL++ +K PI+ GSMGK VE+I Sbjct: 295 SAGEPLNREVIDTFAKHFGVEVRDGYGQTENTLLVGVMKGMPIKPGSMGKPTPGNRVEII 354 Query: 371 DGEGNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWF 430 D G P P G VGDIAVH+ PALFK YYKDPER G+Y++TGD+ARKDEDGYFWF Sbjct: 355 DENGEPCPPGQVGDIAVHIETPALFKYYYKDPERTAMQFRGDYYITGDKARKDEDGYFWF 414 Query: 431 EGRGDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDH-SAG 489 EGRGDDIIIS+GYTIGPFEVE+AL+KH VKECAVVASPDE+RG VVKAFVVL+D Sbjct: 415 EGRGDDIIISAGYTIGPFEVEDALVKHPAVKECAVVASPDEVRGHVVKAFVVLRDGVDKD 474 Query: 490 TPELVKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAA 541 P L+ LQ HVK+ TAPYKYPRKIEF+ DLPKTASGKIRRVELR++E + A Sbjct: 475 DPSLIPALQEHVKQLTAPYKYPRKIEFVDDLPKTASGKIRRVELREREARLA 526 >A4IRT7.1 A4IRT7_GEOTN Acetyl-CoA synthetase Length = 531 Score = 657 bits (1694), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/532 (61%), Positives = 387/532 (72%), Gaps = 9/532 (1%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPA---DRIALKWLQENGNYEEITYGDLMKQANRLAGGLS 70 + ++ IAPE YNLTSEME H A +RIALKW E G +EITYG LM +AN++ Sbjct: 1 MKREELIAPERYNLTSEMERHAAAHPERIALKWENERGETKEITYGRLMARANQIGNAFL 60 Query: 71 GLGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIV 130 L +GD+VLVMVPR I AY +YL LK GL VIPSSEMLR KDL YRL H E +A++ Sbjct: 61 SRRLEKGDKVLVMVPRLIEAYEVYLGALKAGLVVIPSSEMLRTKDLEYRLSHGEVKAIVA 120 Query: 131 WSEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIA 190 + E I G L + ++G E + GW L+ M + E+ A + RDD+A Sbjct: 121 YEPYLDEFAPIDGVDKLL--KFSIGEHEHD---GWVRLDAAMAAESETLAAADTSRDDMA 175 Query: 191 ILAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLT 250 L+YTSGTTGNPK VVH HGW YAHL+ + WL I E+D VWATA PGWQKWIWSPFL+ Sbjct: 176 FLSYTSGTTGNPKGVVHCHGWAYAHLRTAAKNWLCIEETDLVWATAGPGWQKWIWSPFLS 235 Query: 251 VLGNGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAV 310 VLG+GATGFVY G F P+++LQLL+ + VLCCTPTEYRLMAK + RYDLS L AV Sbjct: 236 VLGSGATGFVYYGRFEPEKYLQLLEKYQVNVLCCTPTEYRLMAKVPDIGRYDLSHLHSAV 295 Query: 311 SAGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVI 370 SAGEPLN+EVI+TF +HF I +RDGYGQTE+TL++ +K I+ GSMGK VE+I Sbjct: 296 SAGEPLNREVIDTFAKHFGIQVRDGYGQTENTLLVGVMKGMDIKPGSMGKPTPGNRVEII 355 Query: 371 DGEGNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWF 430 D G P P GVVGDIAVH+ PALFK YYKDPER G+Y++TGD+ARKDE+GYFWF Sbjct: 356 DENGEPCPVGVVGDIAVHVETPALFKYYYKDPERTAMQFRGDYYITGDKARKDENGYFWF 415 Query: 431 EGRGDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDH-SAG 489 EGRGDDIIISSGYTIGPFEVE+AL+KH VKECAVVASPDE+RG VVKAFVVL+D Sbjct: 416 EGRGDDIIISSGYTIGPFEVEDALVKHPAVKECAVVASPDEVRGHVVKAFVVLRDGVDPN 475 Query: 490 TPELVKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAA 541 P L+ LQ HVK+ TAPYKYPRKIEF+ DLPKT SGKIRRVELR++E + A Sbjct: 476 DPSLIPALQEHVKQLTAPYKYPRKIEFVDDLPKTVSGKIRRVELREREARLA 527 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,546,919,333 Number of extensions: 160079054 Number of successful extensions: 471053 Number of sequences better than 1.0e-05: 16448 Number of HSP's gapped: 424781 Number of HSP's successfully gapped: 19304 Length of query: 543 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 402 Effective length of database: 1,732,333,088 Effective search space: 696397901376 Effective search space used: 696397901376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)