BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0002.p (847 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGE6.1 B3KGE6_9BACI Alpha-mannosidase EC=3.2.1.24 748 0.0 C1KWV9.1 C1KWV9_LISMC Putative alpha-mannosidase 277 4e-72 Q4EHD3.1 Q4EHD3_LISMO Alpha-D-mannosidase 275 2e-71 Q71Y05.1 Q71Y05_LISMF Alpha-D-mannosidase 274 3e-71 A0AKC1.1 A0AKC1_LISW6 Complete genome EC=3.2.1.24 274 4e-71 >B3KGE6.1 B3KGE6_9BACI Alpha-mannosidase EC=3.2.1.24 Length = 868 Score = 748 bits (1930), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/485 (71%), Positives = 402/485 (82%) Query: 1 MERIRRFIRELSERQWLEQQKLEGWDIQAGVYTVPGQYDGMRPYTEGGDFTLFPSVQGTT 60 MERIRRFIRELSERQWLE +L W+I Y VPG Y+ PY EG +F FPS QGTT Sbjct: 1 MERIRRFIRELSERQWLESTELRDWNITRSAYMVPGVYEETAPYPEGQNFNRFPSTQGTT 60 Query: 61 YFFRRTLAVPAEWAGQRAGLIFESGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLEL 120 YFFRR L +P +WA GL+F+SGGEGLLR+NG S QGLDRNHTF L+P G +EL Sbjct: 61 YFFRRNLELPLKWADGAIGLVFQSGGEGLLRINGASYQGLDRNHTFVKLDPKRTGMNMEL 120 Query: 121 EIELFDPIPEPVDPLNQQAVIQPPVRAIRTMLVLVNEAVQSLMYSAVIVRDAAVLLPEAD 180 EIELFDPIPEPVDPLNQQAVIQPP+ ++++LV VN VQSLMY+A ++RD+ +LLP+ D Sbjct: 121 EIELFDPIPEPVDPLNQQAVIQPPITRVQSLLVRVNVPVQSLMYTATVIRDSLILLPDTD 180 Query: 181 MRRTRMLEALYRVIDGFLNMDETAVREGSAVAALEKKLRADIAGIGGNAEGTIHMVGQSH 240 MRR RM+EALY+ +D F+ +++ A+R+G+ V A+E L+ +A IGG+AEGT HMVGQSH Sbjct: 181 MRRIRMMEALYQAMDRFVGLNDAAIRDGAEVTAIEHDLKQSVAAIGGHAEGTEHMVGQSH 240 Query: 241 IDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKER 300 IDIAWLWP RETVRKTSRTFST++ALM EYPE+++AQSQP L+Q+LKD+DP LY +VK R Sbjct: 241 IDIAWLWPVRETVRKTSRTFSTMDALMNEYPEFKYAQSQPLLYQFLKDHDPELYERVKAR 300 Query: 301 IREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASL 360 I+EGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFY EEFG TS IEWLPDTFGYCASL Sbjct: 301 IKEGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYQEEFGLTSQIEWLPDTFGYCASL 360 Query: 361 PQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSMLSYLNHGVNENTLPKDVHDH 420 PQIL+HGG+RYFMTTKLGWNDTNVFPYDLFHWVGIDGT MLSYLNHGVNENTLPKDVH+H Sbjct: 361 PQILKHGGIRYFMTTKLGWNDTNVFPYDLFHWVGIDGTPMLSYLNHGVNENTLPKDVHEH 420 Query: 421 WQSFREKNIHNEQMLLYXXXXXXXXVTREMLEYIRRAELMVGQPASTFSNAAGFFSGIGE 480 WQS+R+K +H EQMLLY VTREMLEYI RAELMVGQPAS +S AA FF GI Sbjct: 421 WQSYRQKAVHPEQMLLYGHGDGGGGVTREMLEYINRAELMVGQPASKYSTAAEFFDGIDA 480 Query: 481 RQPQV 485 QP++ Sbjct: 481 AQPKL 485 >C1KWV9.1 C1KWV9_LISMC Putative alpha-mannosidase Length = 1036 Score = 277 bits (708), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 157/415 (37%), Positives = 229/415 (55%), Gaps = 21/415 (5%) Query: 84 SGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQP 143 SG E LL VNGE QG+D+NH S G+ +EL L+ + P Q ++ Sbjct: 101 SGFESLLFVNGEPYQGVDQNHREVLFPDSFAGKKVELVFRLWSGLEGGGTPTIQTHQLK- 159 Query: 144 PVRAIRTMLVLVNEAVQSLMYSAVIVRDAAVLLPEADMRRTRMLEALYRVIDGFLNMDET 203 + +N + L +++ L E + +L+A+ R +L +D + Sbjct: 160 -----EAFIGYLNLVIDDLYFTSKATLKTLDQLEEKNPTHAPLLQAINR---AYLAIDWS 211 Query: 204 ---AVREGSAVAALEKKLRADIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRKTSRTF 260 + + +++A + L+A + + N + VG +HID+AWLW + T K +R+F Sbjct: 212 TPGSEQNLASMATANQILQAALEKMPKNFPIKVAAVGHTHIDVAWLWRLKHTREKAARSF 271 Query: 261 STVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNI 320 STV LME+YPEY F QSQPQL+ Y+K++ P +YAK+K +I EG WE GGMW+E D NI Sbjct: 272 STVLHLMEDYPEYLFLQSQPQLYAYIKEDYPEIYAKIKTKISEGNWEADGGMWLEADCNI 331 Query: 321 PSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWN 380 PSGESL+RQMLYGQ+F EEFGK S WLPD FGY +LPQILR G++ FMTTK+ WN Sbjct: 332 PSGESLVRQMLYGQKFLREEFGKPSSFLWLPDVFGYSWALPQILRKSGIKTFMTTKISWN 391 Query: 381 DTNVFPYDLFHWVGIDGTSMLSYLNHGVNENT---------LPKDVHDHWQSFREKNIHN 431 N P+D F W GIDGT +L++ +N+ +++ W +++K ++ Sbjct: 392 QYNRMPHDTFQWRGIDGTEILTHFITAPEKNSRISTYNGKMTARELVGLWDKYQDKEVNQ 451 Query: 432 EQMLLYXXXXXXXXVTREMLEYIRRAELMVGQPASTFSNAAGFFSGIGERQPQVD 486 E +L Y V REMLE +RR + M G P +A +F+ + E D Sbjct: 452 ELLLAYGYGDGGGGVNREMLEMLRRYDTMPGIPEVKPKSATSYFNDLHETINTTD 506 >Q4EHD3.1 Q4EHD3_LISMO Alpha-D-mannosidase Length = 998 Score = 275 bits (703), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 157/415 (37%), Positives = 228/415 (54%), Gaps = 21/415 (5%) Query: 84 SGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQP 143 SG E LL VNGE QG+D+NH S G+ +EL L+ + P Q ++ Sbjct: 63 SGFESLLFVNGEPYQGVDQNHREVLFPDSFAGKKVELVFRLWSGLEGGGTPTIQTHQLK- 121 Query: 144 PVRAIRTMLVLVNEAVQSLMYSAVIVRDAAVLLPEADMRRTRMLEALYRVIDGFLNMDET 203 + +N + L +++ L E + +L+A+ R +L +D + Sbjct: 122 -----EAFIGYLNLVIDDLYFTSKATLKTLDQLEEKNPTHAPLLQAINR---AYLAIDWS 173 Query: 204 ---AVREGSAVAALEKKLRADIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRKTSRTF 260 + + +++A + L+A + + N + VG +HID+AWLW + T K +R+F Sbjct: 174 TPGSEQNLASMATANQILQAALEKMPKNFPIKVAAVGHTHIDVAWLWRLKHTREKAARSF 233 Query: 261 STVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNI 320 STV LME+YPEY F QSQPQL+ Y+K++ P +YAK+K +I EG WE GGMW+E D NI Sbjct: 234 STVLHLMEDYPEYLFLQSQPQLYAYIKEDYPEIYAKIKTKISEGNWEADGGMWLEADCNI 293 Query: 321 PSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWN 380 PSGESL+RQMLYGQ+F EEFGK S WLPD FGY +LPQILR G++ FMTTK+ WN Sbjct: 294 PSGESLVRQMLYGQKFLREEFGKPSSFLWLPDVFGYSWALPQILRKSGIKTFMTTKISWN 353 Query: 381 DTNVFPYDLFHWVGIDGTSMLSYLNHGVNENT---------LPKDVHDHWQSFREKNIHN 431 N P+D F W GIDGT +L++ +N+ +++ W +++K ++ Sbjct: 354 QYNRMPHDTFQWRGIDGTEILTHFITAPEKNSRISTYNGKMTARELVGLWDKYQDKEVNQ 413 Query: 432 EQMLLYXXXXXXXXVTREMLEYIRRAELMVGQPASTFSNAAGFFSGIGERQPQVD 486 E +L Y V REMLE RR + M G P +A +F+ + E D Sbjct: 414 ELLLAYGYGDGGGGVNREMLEMRRRYDTMPGIPEVKPKSATSYFNDLHETINTTD 468 >Q71Y05.1 Q71Y05_LISMF Alpha-D-mannosidase Length = 1032 Score = 274 bits (700), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 156/409 (38%), Positives = 227/409 (55%), Gaps = 21/409 (5%) Query: 84 SGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQP 143 SG E LL VNGE QG+D+NH S G+ +EL L+ + P Q ++ Sbjct: 97 SGFESLLFVNGEPYQGVDQNHREVLFPDSFAGKKVELVFRLWSGLEGGGTPTIQTHQLK- 155 Query: 144 PVRAIRTMLVLVNEAVQSLMYSAVIVRDAAVLLPEADMRRTRMLEALYRVIDGFLNMDET 203 + +N + L +++ L E + +L+A+ R +L +D + Sbjct: 156 -----EAFIGYLNLVIDDLYFTSKATLKTLDQLEEKNPTYAPLLQAINR---AYLAIDWS 207 Query: 204 ---AVREGSAVAALEKKLRADIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRKTSRTF 260 + + +++A + L+A + + N + VG +HID+AWLW + T K +R+F Sbjct: 208 TPGSEQNLASMATANQILQAALEKMPKNFPIKVAAVGHTHIDVAWLWRLKHTREKAARSF 267 Query: 261 STVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNI 320 STV LME+YPEY F QSQPQL+ Y+K++ P +YAK+K +I EG WE GGMW+E D NI Sbjct: 268 STVLHLMEDYPEYLFLQSQPQLYAYIKEDYPEIYAKIKTKISEGNWEADGGMWLEADCNI 327 Query: 321 PSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWN 380 PSGESL+RQMLYGQ+F EEFGK S WLPD FGY +LPQILR G++ FMTTK+ WN Sbjct: 328 PSGESLVRQMLYGQKFLREEFGKPSSFLWLPDVFGYSWALPQILRKSGIKTFMTTKISWN 387 Query: 381 DTNVFPYDLFHWVGIDGTSMLSYLNHGVNENT---------LPKDVHDHWQSFREKNIHN 431 N P+D F W GIDGT +L++ +N+ +++ W +++K ++ Sbjct: 388 QYNRMPHDTFQWRGIDGTEILTHFITAPEKNSRISTYNGKMTARELVGLWDKYQDKEVNQ 447 Query: 432 EQMLLYXXXXXXXXVTREMLEYIRRAELMVGQPASTFSNAAGFFSGIGE 480 E +L Y V REMLE RR + M G P +A +F+ + E Sbjct: 448 ELLLAYGYGDGGGGVNREMLEMRRRYDTMPGIPEVKPKSATSYFNDLHE 496 >A0AKC1.1 A0AKC1_LISW6 Complete genome EC=3.2.1.24 Length = 1032 Score = 274 bits (700), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 156/415 (37%), Positives = 227/415 (54%), Gaps = 21/415 (5%) Query: 84 SGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQP 143 SG E LL VNGE QG+D+NH S G+ EL L+ + P Q ++ Sbjct: 97 SGFESLLFVNGEPFQGVDQNHREVLFPDSFAGKTTELVFRLWSGLEGGGKPSIQTHQLK- 155 Query: 144 PVRAIRTMLVLVNEAVQSLMYSAVIVRDAAVLLPEADMRRTRMLEALYRVIDGFLNMDET 203 + +N + L +++ L E + +L+A+ R +L +D + Sbjct: 156 -----EAFIGYLNLVIDDLYFTSKATLKTLDQLEEKNPIHAPLLQAINR---AYLAIDWS 207 Query: 204 AVREGSAVAALEKK---LRADIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRKTSRTF 260 ++++E L+A I + N + VG +HID+AWLW + T K +R+F Sbjct: 208 TPGSAQNLSSMETANQILQAAIKEMPKNFPIKVAAVGHTHIDVAWLWRLKHTREKAARSF 267 Query: 261 STVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNI 320 STV LME+YPEY F QSQPQL+ Y+K++ P +YAK+K +I++G WE GGMW+E D NI Sbjct: 268 STVLHLMEDYPEYLFLQSQPQLYAYMKEDYPEIYAKIKAKIKDGNWEADGGMWLEADCNI 327 Query: 321 PSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWN 380 PSGESL+RQMLYGQ+F EEFGK S WLPD FGY +LPQILR G++ FMTTK+ WN Sbjct: 328 PSGESLVRQMLYGQKFLREEFGKPSSFLWLPDVFGYSWALPQILRKSGIKTFMTTKISWN 387 Query: 381 DTNVFPYDLFHWVGIDGTSMLSYLNHGVNENT---------LPKDVHDHWQSFREKNIHN 431 N P+D F W GIDGT +L++ +N+ +++ W +++K ++ Sbjct: 388 QYNRMPHDTFKWRGIDGTEILTHFITAPEKNSRISTYNGKMTARELVGLWDKYQDKEVNQ 447 Query: 432 EQMLLYXXXXXXXXVTREMLEYIRRAELMVGQPASTFSNAAGFFSGIGERQPQVD 486 E +L Y V REMLE RR ++M G P +A +F+ + + D Sbjct: 448 ELLLAYGYGDGGGGVNREMLEMRRRYDVMPGIPEVKPKSATNYFNDLHQTIENTD 502 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 5,183,511,551 Number of extensions: 228580061 Number of successful extensions: 444565 Number of sequences better than 1.0e-05: 427 Number of HSP's gapped: 444347 Number of HSP's successfully gapped: 428 Length of query: 847 Length of database: 3,060,696,203 Length adjustment: 145 Effective length of query: 702 Effective length of database: 1,694,649,028 Effective search space: 1189643617656 Effective search space used: 1189643617656 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 136 (57.0 bits)