BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0011.p (253 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D7N4.1 B1D7N4_9BACL Short-chain dehydrogenase/reductase SDR 370 e-101 Q2BDD1.1 Q2BDD1_9BACI DltE 365 3e-99 A7Z8D1.1 A7Z8D1_BACA2 DltE 359 2e-97 Q65MY8.1 Q65MY8_BACLD DltE 312 1e-83 C2BZ98.1 C2BZ98_LISGR Short-chain dehydrogenase/reductase SDR 280 6e-74 >B1D7N4.1 B1D7N4_9BACL Short-chain dehydrogenase/reductase SDR Length = 253 Score = 370 bits (950), Expect = e-101, Method: Compositional matrix adjust. Identities = 172/234 (73%), Positives = 199/234 (85%) Query: 20 AFAERFIQAGNQVIVCGRHEDVLRNAQDKFPGLITRVCDLNVESERIAMFDWVTANYPEV 79 AFAERF++AGN VIV GR E VL+ AQ+K PGLIT+ DL+ E++RIA+FDWVTANYPE Sbjct: 20 AFAERFMKAGNTVIVTGRREQVLQAAQEKLPGLITKASDLSNEAQRIALFDWVTANYPET 79 Query: 80 NVLVNNAGTQQRFNVLKVDAKHNWSYFNQEITTNLDAPFHLSLLXXXXXXXXXXXXIINV 139 NVLVNNAG QQRF+VLK D K +W YFN+EI TN++APFHL++L IINV Sbjct: 80 NVLVNNAGIQQRFSVLKADVKSDWQYFNKEIATNIEAPFHLAMLFAPFFAAKENAAIINV 139 Query: 140 TSGLAFTPFAIAPIYSATKAALHSFTMSLRHQLSETSVEVIEVAPPAVNTELGGAGLHTH 199 TSGLAFTPFAIAPIYSATKAALHSFT+SLRHQLS TSVEVIEVAPPAVNT+LGG+GLH H Sbjct: 140 TSGLAFTPFAIAPIYSATKAALHSFTISLRHQLSATSVEVIEVAPPAVNTDLGGSGLHVH 199 Query: 200 GEPLDAFADGIFRGLEEGKQEIGYGTSVDRLRMSRDEVDEYTANMYQATKGMIE 253 GEPLDAFADGIF+GL+EGK EIGYGTSVDRLRMSRD++D+Y +MY ATK M++ Sbjct: 200 GEPLDAFADGIFQGLQEGKSEIGYGTSVDRLRMSRDDIDKYAESMYHATKNMLD 253 >Q2BDD1.1 Q2BDD1_9BACI DltE Length = 253 Score = 365 bits (936), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 173/234 (73%), Positives = 193/234 (82%) Query: 20 AFAERFIQAGNQVIVCGRHEDVLRNAQDKFPGLITRVCDLNVESERIAMFDWVTANYPEV 79 AF ERFI AGN VIVCGR E VL+ A++KFPGLI RVCDL VESER A+FDWV N+PEV Sbjct: 20 AFTERFINAGNTVIVCGRRESVLQEAKEKFPGLIIRVCDLAVESERSALFDWVEENHPEV 79 Query: 80 NVLVNNAGTQQRFNVLKVDAKHNWSYFNQEITTNLDAPFHLSLLXXXXXXXXXXXXIINV 139 NVLVNNAG QQRFNVL DAK +W YFN+EITTN++APFHLS+L IINV Sbjct: 80 NVLVNNAGIQQRFNVLTADAKKDWGYFNKEITTNIEAPFHLSMLFAPFLAKKTEAAIINV 139 Query: 140 TSGLAFTPFAIAPIYSATKAALHSFTMSLRHQLSETSVEVIEVAPPAVNTELGGAGLHTH 199 TSGLAFTP IAPIYSATKAA HSFT+SLRHQLS T VEVIE+APPAVNT+LGGAGLHT Sbjct: 140 TSGLAFTPLVIAPIYSATKAAFHSFTVSLRHQLSGTPVEVIEIAPPAVNTDLGGAGLHTQ 199 Query: 200 GEPLDAFADGIFRGLEEGKQEIGYGTSVDRLRMSRDEVDEYTANMYQATKGMIE 253 GEPL+ FADGIF+GL EGK+EIGYG+SV+RLRMSRDE+D +TA MYQA K IE Sbjct: 200 GEPLNEFADGIFKGLAEGKEEIGYGSSVERLRMSRDELDAHTAKMYQAMKHTIE 253 >A7Z8D1.1 A7Z8D1_BACA2 DltE Length = 253 Score = 359 bits (921), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 170/234 (72%), Positives = 196/234 (83%) Query: 20 AFAERFIQAGNQVIVCGRHEDVLRNAQDKFPGLITRVCDLNVESERIAMFDWVTANYPEV 79 AFAERF++AGN+VIV GR E L+ A++ FP LIT V DL++ SERI++F+WV NYPEV Sbjct: 20 AFAERFLKAGNKVIVTGRREHALQKAKETFPDLITYVSDLSIPSERISLFEWVKENYPEV 79 Query: 80 NVLVNNAGTQQRFNVLKVDAKHNWSYFNQEITTNLDAPFHLSLLXXXXXXXXXXXXIINV 139 NVLVNNAG QQRF+VLK DAK NW YFN+EITTN++APFHLS+L INV Sbjct: 80 NVLVNNAGIQQRFHVLKADAKDNWDYFNKEITTNIEAPFHLSMLFAPFFAGKEDAAFINV 139 Query: 140 TSGLAFTPFAIAPIYSATKAALHSFTMSLRHQLSETSVEVIEVAPPAVNTELGGAGLHTH 199 +SGLAFTP AIAPIYSATKAALHSFTMSLRHQLS++SVEVIEVAPPAVNT+LGGAGLHT Sbjct: 140 SSGLAFTPLAIAPIYSATKAALHSFTMSLRHQLSDSSVEVIEVAPPAVNTDLGGAGLHTQ 199 Query: 200 GEPLDAFADGIFRGLEEGKQEIGYGTSVDRLRMSRDEVDEYTANMYQATKGMIE 253 GEPLD FADGIF+GL+EGKQEIGYG+S +RLRMSRD+ DE+TANMY A K IE Sbjct: 200 GEPLDEFADGIFKGLKEGKQEIGYGSSEERLRMSRDKADEHTANMYNAMKHTIE 253 >Q65MY8.1 Q65MY8_BACLD DltE Length = 254 Score = 312 bits (800), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 148/235 (62%), Positives = 180/235 (76%), Gaps = 1/235 (0%) Query: 20 AFAERFIQAGNQVIVCGRHEDVLRNAQDKFPGLITRVCDLNVESERIAMFDWVTANYPEV 79 AFAERFI+AGN VIVCGR E L+ A++K P LIT VCD+ VE+ER+A+F+W+ ++P V Sbjct: 20 AFAERFIKAGNTVIVCGRRESKLKEAKEKHPELITCVCDVAVETERLALFEWIKEHHPGV 79 Query: 80 NVLVNNAGTQQRFNVLKVDAKHNWSYFNQEITTNLDAPFHLSLLXXXX-XXXXXXXXIIN 138 NVLVNNAG QQR+++LK D NW+ +N+EIT N++AP HL++L IIN Sbjct: 80 NVLVNNAGIQQRYHILKADVIDNWADYNKEITVNMEAPIHLAMLFSRYFAEKAGKTAIIN 139 Query: 139 VTSGLAFTPFAIAPIYSATKAALHSFTMSLRHQLSETSVEVIEVAPPAVNTELGGAGLHT 198 V+SGLAFTP A+APIYS+TKAALHSFTMSLRHQLSE VEVIEVAPPAVNT+LGG GLH Sbjct: 140 VSSGLAFTPMAVAPIYSSTKAALHSFTMSLRHQLSEAGVEVIEVAPPAVNTDLGGVGLHD 199 Query: 199 HGEPLDAFADGIFRGLEEGKQEIGYGTSVDRLRMSRDEVDEYTANMYQATKGMIE 253 G PLD FAD IF+GLEEG+ EIGY S LRMSRDE+DE + +Y + K IE Sbjct: 200 SGAPLDEFADAIFKGLEEGRLEIGYAVSEKALRMSRDEIDESVSQLYSSLKHSIE 254 >C2BZ98.1 C2BZ98_LISGR Short-chain dehydrogenase/reductase SDR Length = 249 Score = 280 bits (717), Expect = 6e-74, Method: Compositional matrix adjust. Identities = 126/233 (54%), Positives = 169/233 (72%) Query: 21 FAERFIQAGNQVIVCGRHEDVLRNAQDKFPGLITRVCDLNVESERIAMFDWVTANYPEVN 80 AER+++ G++VI+CGR+E L + Q P +ITR CDL E+ERIA+ DW++A++PE N Sbjct: 17 LAERYLKQGDRVIICGRNEQKLADVQAAHPEVITRKCDLTNEAERIALVDWISADHPETN 76 Query: 81 VLVNNAGTQQRFNVLKVDAKHNWSYFNQEITTNLDAPFHLSLLXXXXXXXXXXXXIINVT 140 +LVNNAG QQRFN+LK D ++NW Y+ QEI N +A HL++L I+NV+ Sbjct: 77 ILVNNAGIQQRFNLLKADVRNNWDYYLQEIKANEEATIHLAILFSQLFAEKEQATIVNVS 136 Query: 141 SGLAFTPFAIAPIYSATKAALHSFTMSLRHQLSETSVEVIEVAPPAVNTELGGAGLHTHG 200 SGLA P I+PIYSA+KAA+H+FTM+LRHQLSET+++VIE+ PPAVNT+LGG GLH +G Sbjct: 137 SGLAIIPMVISPIYSASKAAVHAFTMALRHQLSETAIKVIEILPPAVNTDLGGTGLHNNG 196 Query: 201 EPLDAFADGIFRGLEEGKQEIGYGTSVDRLRMSRDEVDEYTANMYQATKGMIE 253 E D FAD +FR ++ GK+EIG+ S L MSR E++E A MYQ K IE Sbjct: 197 EDADEFADSVFRDMQAGKEEIGFAHSAKVLEMSRKEINETVAGMYQKMKAEIE 249 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,265,434,062 Number of extensions: 47340364 Number of successful extensions: 144317 Number of sequences better than 1.0e-05: 932 Number of HSP's gapped: 148593 Number of HSP's successfully gapped: 933 Length of query: 253 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 120 Effective length of database: 1,807,701,208 Effective search space: 216924144960 Effective search space used: 216924144960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)