BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0005.p (749 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3I4.1 B3K3I4_9BACI Pectate lyase/Amb allergen 941 0.0 B7AQA3.1 B7AQA3_9BACE Putative uncharacterized protein 612 e-173 Q97KW2.1 Q97KW2_CLOAB Pectate lyase related protein, secreted 554 e-155 C4Z0Y4.1 C4Z0Y4_9FIRM Polysaccharide Lyase Family 1 modular prot... 533 e-149 C4Z0Y3.1 C4Z0Y3_9FIRM Polysaccharide Lyase Family 1 modular prot... 527 e-147 >B3K3I4.1 B3K3I4_9BACI Pectate lyase/Amb allergen Length = 683 Score = 941 bits (2433), Expect = 0.0, Method: Compositional matrix adjust. Identities = 451/561 (80%), Positives = 504/561 (89%), Gaps = 1/561 (0%) Query: 21 MKNRAAKITGIVLLFSLALSLIAGGWAN-TTVYAAGLSITGSGGWNETAYVEWSPVSNVQ 79 MKN AK+TG+V+LFSLALSLIAGGW N TTV+AAGLSI G GGWNETAY+EWSPVSN + Sbjct: 1 MKNSVAKVTGMVVLFSLALSLIAGGWVNKTTVHAAGLSIIGGGGWNETAYIEWSPVSNAE 60 Query: 80 GYSVYVKPASASDSQYQQLNNELIRRYPSYWRADAVGLAAGSYVMKVEXXXXXXXXXXXX 139 GY+VYVK A+A DS YQQ++NELIR+YPSYWRADAVGLAAG+YVMKVE Sbjct: 61 GYNVYVKHANAPDSSYQQMDNELIRQYPSYWRADAVGLAAGNYVMKVEARLSDGSTVSVV 120 Query: 140 XXXXXXTSYDRAGFAFSASSPYGTGSGAYNENGTLKNGAQVLYITSQNAQTVTLPVKINS 199 +DR+GFAFS+ SPYGTGSGAYN++GTLK+GAQV+Y+TS+ A+TVTL VKINS Sbjct: 121 SNNLSVAPHDRSGFAFSSDSPYGTGSGAYNDDGTLKDGAQVIYVTSETARTVTLDVKINS 180 Query: 200 SGAVQTGVGLGGILTLRQKGYDTTPLAIRIIGKVTAADLSGQLNSSGYLEVKGKNNYSQM 259 SG VQTGVG+G ILTLRQKGYDTTPLAIR IGKVT D++GQLNSSGYL+VKGKN+YS+M Sbjct: 181 SGGVQTGVGIGDILTLRQKGYDTTPLAIRFIGKVTDDDMNGQLNSSGYLQVKGKNDYSEM 240 Query: 260 NMTIEGIGNDAYAYGWGMLLRYVGNVEVRNLGVMLFPDDGISMDTGNVNVWVHNNDIFYG 319 NMTIEGIG+DAYAYGWG+LLRYVGNVEVRNLG+MLFPDDGISMDTGNVNVWVHNND+FYG Sbjct: 241 NMTIEGIGDDAYAYGWGLLLRYVGNVEVRNLGLMLFPDDGISMDTGNVNVWVHNNDLFYG 300 Query: 320 SAGSDADQAKGDGSTDLKKGSTYITISYNHYWDSGKASLVGLSEPAEFFVTFHHNWFDHS 379 +AG DADQAKGDGSTDLKKGSTYITISYNHYWDSGKA+LVGLSE EFFVTFHHNWFDHS Sbjct: 301 TAGGDADQAKGDGSTDLKKGSTYITISYNHYWDSGKAALVGLSESEEFFVTFHHNWFDHS 360 Query: 380 DSRHPRIRVASVHVYNNFYDGVSKYGVGVTTGASAFVESNVFRHAKYPMMIALQGTDALG 439 DSRHPRIR+ASVH+YNNFYDGVSKYGVGVTTGASAFVESN FRHAKYPM+ +LQGTDALG Sbjct: 361 DSRHPRIRLASVHIYNNFYDGVSKYGVGVTTGASAFVESNYFRHAKYPMLSSLQGTDALG 420 Query: 440 EGTFSGENGGMIKAYNNTVVDAASLIYANSNAGTAPANTTSFDAYLASSRNESVPSSYKA 499 EGTFSGE+GG+IKAYNN +VDA SLIYANSN GTAPAN TSFDAYLASSRNE+VPSSYKA Sbjct: 421 EGTFSGEDGGVIKAYNNRIVDADSLIYANSNTGTAPANATSFDAYLASSRNETVPSSYKA 480 Query: 500 LVGGTAYNNFDTSADTGVSASDIDSVSTVEQIVTAEAGRMNGGDFTWAFNDSVDDASYAL 559 VGGTAYNNFDT+ DTGV+++DID+VS VEQIVTA+AGR+N GDFTW FND+VDD SY L Sbjct: 481 FVGGTAYNNFDTTVDTGVNSADIDNVSDVEQIVTAQAGRLNNGDFTWEFNDAVDDTSYTL 540 Query: 560 NSALMSAIRSYTTQLVSVGGN 580 N+ALM+ I SYT+ LVSVGGN Sbjct: 541 NTALMAKISSYTSGLVSVGGN 561 Score = 159 bits (402), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 78/106 (73%), Positives = 90/106 (84%), Gaps = 1/106 (0%) Query: 645 HNFTTAGTASSFFNIKGNLSTTKGTVVYNGLTLTQCLKIESTTSIQFTAANASILTLVFN 704 HNFTT+G AS FF+I GNLSTTKGT VYNGLTLTQCLKIES+T I FT+ + LTLVFN Sbjct: 578 HNFTTSGLASDFFHIVGNLSTTKGTAVYNGLTLTQCLKIESSTRITFTSTETATLTLVFN 637 Query: 705 S-EGTKIKVDGTSYPITNGIATVSLAAGAHTITKDSTANLYYMELD 749 S +GTKIK+DGTSYP+TNG+ +V LA GAHTITKD T NL+YMEL+ Sbjct: 638 SADGTKIKIDGTSYPMTNGMVSVPLAPGAHTITKDHTTNLFYMELE 683 >B7AQA3.1 B7AQA3_9BACE Putative uncharacterized protein Length = 698 Score = 612 bits (1578), Expect = e-173, Method: Compositional matrix adjust. Identities = 310/562 (55%), Positives = 394/562 (70%), Gaps = 12/562 (2%) Query: 23 NRAAKITGIVLLFSLALSLIAGGWANTTVYAAG--LSITGSGGWNETAYVEWSPVSNVQG 80 ++ K+ +L F + S ++ + T A+ LSIT + GW ETAY EW+PV G Sbjct: 41 RKSKKLMAYLLAFLMVASAMSPLFVKETFAASAKTLSITEAAGWLETAYAEWTPVDGAAG 100 Query: 81 YSVYVKPASASDSQYQQLNNELIRRYPSYWRADAVGLAAGSYVMKVEXXXXXXXXXXXXX 140 Y YVK AS SD+ + QL++ELIR+Y YWRADA GLAAGSYVM + Sbjct: 101 YMAYVKKASESDAAFVQLDSELIRQYAGYWRADAPGLAAGSYVMNIVAVMKDGSRISCKT 160 Query: 141 XXXXXTSYDRAGFAFSASSPYGTGSGAYNENGTLKNGAQVLYITSQNAQTVTLPVKINSS 200 ++DR+GFAFS+ S YGTGSGAYN++GTLK+ AQV+Y+T+ A+T V Sbjct: 161 GTLDVKAHDRSGFAFSSKSQYGTGSGAYNDDGTLKSNAQVVYVTADTAKTCKAVV----- 215 Query: 201 GAVQTGVGLGGILTLRQKGYDTTPLAIRIIGKVTAADLSGQLNSSGYLEVKGKNNYSQMN 260 G + G IL +QK TP+ RIIG+V+ DL +SS +++KGK+ Y+QMN Sbjct: 216 GGKEI-TGFQSILDAKQKDTTGTPIDFRIIGRVSLNDLDHISSSSEGIQIKGKSAYAQMN 274 Query: 261 MTIEGIGNDAYAYGWGMLLRYVGNVEVRNLGVMLFPDDGISMDTGNVNVWVHNNDIFYGS 320 +T+EGIG DA +G+G L+R GNVE RN +M F DDGIS+DT N N+WVHN DIFYGS Sbjct: 275 ITVEGIGEDAAVHGFGFLVRNAGNVEFRNFALMAFMDDGISLDTKNCNIWVHNMDIFYGS 334 Query: 321 AGSDADQAKGDGSTDLKKGSTYITISYNHYWDSGKASLVGLSEPAEFFVTFHHNWFDHSD 380 G DADQAKGDGS D+K ST +TISY H+WDSGK SL G+S+ AEF VT+HHNWFDHSD Sbjct: 335 TGGDADQAKGDGSVDVKGASTNVTISYVHFWDSGKCSLCGMSDSAEFLVTYHHNWFDHSD 394 Query: 381 SRHPRIRVASVHVYNNFYDGVSKYGVGVTTGASAFVESNVFRHAKYPMMIALQGTDALGE 440 SRH RIRVASVH+YNN+YDG +KYGVG T G+SAFVE+N FR+ K PMM +LQG+DA + Sbjct: 395 SRHARIRVASVHLYNNYYDGNAKYGVGTTKGSSAFVEANYFRNCKNPMMSSLQGSDARAD 454 Query: 441 -GTFSGENGGMIKAYNNTVVDAASLIYANSNAGTAPANTTSFDAYLASSRNESVPSSYKA 499 GTFSGENGGMIKAY N V A++L+YANS+AGT A++ SFDAYLASSR+E VPSSY Sbjct: 455 KGTFSGENGGMIKAYGNVVEGASNLVYANSDAGTMSADSKSFDAYLASSRDEQVPSSYTT 514 Query: 500 LVGGTAYNNFDTSA--DTGVSASDIDSVSTVEQIVTAEAGRMNGGDFTWAFNDSVDDASY 557 + GGT YNNFDTS+ D GV AS +D V +VT AGRMNGGDF W F+ S DD +Y Sbjct: 515 VAGGTKYNNFDTSSSYDIGVKASAVDKAEDVPAVVTKYAGRMNGGDFKWTFSAS-DDTNY 573 Query: 558 ALNSALMSAIRSYTTQLVSVGG 579 ++++AL SA+ SY + + ++GG Sbjct: 574 SVDTALKSAVVSYKSSVRAIGG 595 >Q97KW2.1 Q97KW2_CLOAB Pectate lyase related protein, secreted Length = 564 Score = 554 bits (1428), Expect = e-155, Method: Compositional matrix adjust. Identities = 282/535 (52%), Positives = 370/535 (69%), Gaps = 20/535 (3%) Query: 51 VYAAGLSITGSGGWNETAYVEWSPVSNVQGYSVYVKPASASDSQYQQLNNELIRRYPSYW 110 V + +SI S G E+A VEWSPV Y+VY K A A DS Y ++++ELIR+YPS + Sbjct: 44 VETSAVSILESEGSLESANVEWSPVQGAASYNVYYKAADALDSSYTKIDDELIRQYPSSF 103 Query: 111 RADAVGLAAGSYVMKVEXXXXXXXXXXXXXXXXXXT--SYDRAGFAFSASSPYGTGSGAY 168 RAD +GLAAG+YV+K+ T + R GFAFS +SP T SG Y Sbjct: 104 RADVLGLAAGNYVLKIVPVVNGVDAINEEATTQALTVKANKREGFAFSPASPMKTSSGGY 163 Query: 169 NENGTLKNGAQVLYITSQNAQTVTLPVKINSSGAVQTGVGLGGILTLRQKGYDTTPLAIR 228 N++GT+ + A++LYIT+ N TVT V N+ G T GL IL RQKGYD +PL IR Sbjct: 164 NDDGTVASNAEILYITASNVNTVTENVVTNAKGTKTTCTGLVNILAGRQKGYDKSPLIIR 223 Query: 229 IIGKVTAADLSGQLNSSGYLEVKGKNNYSQMNMTIEGIGNDAYAYGWGMLLRYVGNVEVR 288 +IG++ A+D++G LN Y+++KG N+T EG+G DA AYGWG L+R N+E+R Sbjct: 224 MIGEIKASDITG-LNEHTYIQLKG-----CYNVTFEGVGKDATAYGWGFLIRDARNLELR 277 Query: 289 NLGVMLFPDDGISMDTGNVNVWVHNNDIFYGSAGSDADQAKGDGSTDLKKGSTYITISYN 348 N+G+M+F DD IS+DT N N+WVHNND+FYG+AG DADQ KGDGS D+K+ ST++++SYN Sbjct: 278 NVGIMMFGDDAISLDTDNQNIWVHNNDLFYGAAGHDADQVKGDGSCDIKQDSTFVSVSYN 337 Query: 349 HYWDSGKASLVGLSEPAEFFVTFHHNWFDHSDSRHPRIRVASVHVYNNFYDGVSKYGVGV 408 H+ DSGK+SL G+ + ++VT+HHNWFDHSDSRHPRIRV SVHVYNN++DG +KYGVGV Sbjct: 338 HFHDSGKSSLCGMQDTQNYYVTYHHNWFDHSDSRHPRIRVGSVHVYNNYFDGNAKYGVGV 397 Query: 409 TTGASAFVESNVFRHAKYPMMIALQGTDAL--GEGTFSGENGGMIKAYNNTVVDAASLIY 466 T GASAFVE+N FR KYPM+ +LQGTD +G FSGE+GGMIKAYNN + A L+Y Sbjct: 398 TKGASAFVEANYFRDCKYPMLSSLQGTDIFYDSKGDFSGEDGGMIKAYNNKIEGATRLVY 457 Query: 467 ANSNAGTAPANTTSFDAYLASSRNESVPSSYKALVGGTAYNNFDTSADTGVSASDIDSVS 526 AN + FDAYLASSRNE VPS+YK LVGGT YNNFDTS + + DS Sbjct: 458 ANQDP-------IQFDAYLASSRNEVVPSTYKTLVGGTTYNNFDTS--SVMYKYQPDSPD 508 Query: 527 TVEQIVTAEAGRMNGGDFTWAFNDSVDDASYALNSALMSAIRSYTTQLVSVGGNL 581 V++ VT+ AGR++GGD + F D+ DD S +N LM+ +++Y++ LVS G + Sbjct: 509 DVKESVTSYAGRVDGGDLIFNFTDA-DDTSSVINPDLMAKVKNYSSTLVSDGSKV 562 >C4Z0Y4.1 C4Z0Y4_9FIRM Polysaccharide Lyase Family 1 modular protein with two candidate pectate lyase domains Length = 1286 Score = 533 bits (1372), Expect = e-149, Method: Compositional matrix adjust. Identities = 276/531 (51%), Positives = 361/531 (67%), Gaps = 10/531 (1%) Query: 58 ITGSGGWNETAYVEWSPVSNVQGYSVYVKPASASDSQYQQLNNELIRRYPSYWRADAVGL 117 I G+ GW E+AY +WS + +GY+ Y+K AS SDS Y +++NELIR+Y +Y+R DAVGL Sbjct: 44 IDGAAGWLESAYAQWSIDNQAEGYTAYIKKASQSDSAYARIDNELIRKYKNYYRVDAVGL 103 Query: 118 AAGSYVMKVEXXX--XXXXXXXXXXXXXXXTSYDRAGFAFSASSPYGTGSGAYNENGTLK 175 AAG YV+KV +SYDR+GFAFS+ S Y TGSGAYNE+GTLK Sbjct: 104 AAGDYVIKVVPVKNGKEVTDKAQVTKTLNVSSYDRSGFAFSSESKYKTGSGAYNEDGTLK 163 Query: 176 NGAQVLYITSQNAQTVTLPVKINSSGAVQTGVG--LGGILTLRQKGYDTTPLAIRIIGKV 233 A VLY+T+ NA+T+ VK TG+ + KG +T L +R+IG V Sbjct: 164 KDAIVLYVTNDNAKTIKASVKEAKGEKEYTGLQTIIDAYTKSASKGIETRALDVRVIGCV 223 Query: 234 TAADLSGQLNSSGYLEVKGKNNYSQMNMTIEGIGNDAYAYGWGMLLRYVGNVEVRNLGVM 293 T A + +SS +++KG + YS +NMTIEGIGNDA G+G LLR GNVE+RN ++ Sbjct: 224 TDAAMDKFSSSSEGVQIKGASAYSNLNMTIEGIGNDATINGFGFLLRNAGNVEMRNFSII 283 Query: 294 LFPDDGISMDTGNVNVWVHNNDIFYGSAGSDADQAKGDGSTDLKKGSTYITISYNHYWDS 353 F DDGIS+DT N NVW+HN D++YG AG DADQAKGDGS D+K S YIT+SY H++DS Sbjct: 284 NFMDDGISLDTANCNVWIHNVDLYYGKAGGDADQAKGDGSIDIKGNSQYITVSYVHFYDS 343 Query: 354 GKASLVGL-SEPAEFFVTFHHNWFDHSDSRHPRIRVASVHVYNNFYDGVSKYGVGVTTGA 412 GK SL G+ SE ++T+HHNWFDHSDSRH R+R SVH+YNN+YDG +KYG G T G+ Sbjct: 344 GKCSLCGMKSESGPNYITYHHNWFDHSDSRHARVRTMSVHMYNNYYDGNAKYGAGSTMGS 403 Query: 413 SAFVESNVFRHAKYPMMIALQGTDALGEGTFSGENGGMIKAYNNTVVDAASLIYANSNAG 472 S F+++N FR+ K PM+ + QGTDALGEGTFSGENGG+IKA N +V A +IYAN+ + Sbjct: 404 SLFIQNNYFRNCKNPMLSSNQGTDALGEGTFSGENGGIIKASGNVIVGAQKIIYANAVSE 463 Query: 473 TA-PANTTSFDAYLASSRNESVPSSYKALVGGTAYNNFDTSADTGVSASDIDSVSTVEQI 531 T AN SFDAYLA S +E VPSSYK + GGT+Y+NFDT+ D GV + +++ V + Sbjct: 464 TGDSANAASFDAYLAKSADEKVPSSYKTVAGGTSYDNFDTTKDLGVKSGSLNNAEDVPSV 523 Query: 532 VTAE--AGRMNGGDFTWAFNDSVDDASYALNSALMSAIRSYT-TQLVSVGG 579 VT+ AG + GG W F+D DD+ YA++ L + + +Y T LVSVGG Sbjct: 524 VTSAKGAGSLGGGVIPWTFSDK-DDSVYAIDKELKATVTNYKNTDLVSVGG 573 >C4Z0Y3.1 C4Z0Y3_9FIRM Polysaccharide Lyase Family 1 modular protein with two candidate pectate lyase domains Length = 1623 Score = 527 bits (1358), Expect = e-147, Method: Compositional matrix adjust. Identities = 273/528 (51%), Positives = 353/528 (66%), Gaps = 10/528 (1%) Query: 61 SGGWNETAYVEWSPVSNVQGYSVYVKPASASDSQYQQLNNELIRRYPSYWRADAVGLAAG 120 S GW E+AY +WS GY+ Y+K A A DS Y +++NELIR+Y YWR DAVGLAAG Sbjct: 48 SAGWLESAYAQWSIDKEADGYTAYIKKADADDSAYTRIDNELIRKYNDYWRVDAVGLAAG 107 Query: 121 SYVMKVEXXXXXXXXXXXXXXXXXXT--SYDRAGFAFSASSPYGTGSGAYNENGTLKNGA 178 YV+KV T SYDR+GFAFS+ S Y TGSGAYNE+GTLK A Sbjct: 108 DYVIKVVPVKDGKEVAGKAKVTDTLTVSSYDRSGFAFSSESKYKTGSGAYNEDGTLKKDA 167 Query: 179 QVLYITSQNAQTVTLPVKINSSGAVQTGVG--LGGILTLRQKGYDTTPLAIRIIGKVTAA 236 VLY+T+ NA+T+ VK TG+ + KG +T L +R+IG VT A Sbjct: 168 IVLYVTNDNAKTIKASVKEAKGEKEYTGLQTIIDAYTKSASKGIETRALDVRVIGCVTDA 227 Query: 237 DLSGQLNSSGYLEVKGKNNYSQMNMTIEGIGNDAYAYGWGMLLRYVGNVEVRNLGVMLFP 296 + +SS +++KG + YS +NMTIEGIGNDA G+G LLR GNVE+RN ++ F Sbjct: 228 AMDKFSSSSEGVQIKGASAYSNLNMTIEGIGNDATINGFGFLLRNAGNVEMRNFSIINFM 287 Query: 297 DDGISMDTGNVNVWVHNNDIFYGSAGSDADQAKGDGSTDLKKGSTYITISYNHYWDSGKA 356 DDGIS+DT N NVW+HN D++YG AG DADQAKGDGS D+K S YIT+SY H++DSGK Sbjct: 288 DDGISLDTANCNVWIHNVDLYYGKAGGDADQAKGDGSIDIKGNSQYITVSYVHFYDSGKC 347 Query: 357 SLVGL-SEPAEFFVTFHHNWFDHSDSRHPRIRVASVHVYNNFYDGVSKYGVGVTTGASAF 415 SL G+ SE ++T+HHNWFDHSDSRH R+R SVH+YNN+YDG +KYG G T G+S F Sbjct: 348 SLCGMKSESGPNYITYHHNWFDHSDSRHARVRTMSVHMYNNYYDGNAKYGAGSTMGSSLF 407 Query: 416 VESNVFRHAKYPMMIALQGTDALGEGTFSGENGGMIKAYNNTVVDAASLIYANSNAGTA- 474 +++N F + KYPM+ + QG+DALGEGTFSGENGG+IKA N +V A +IYAN+ + T Sbjct: 408 IQNNYFNNCKYPMLSSKQGSDALGEGTFSGENGGIIKASGNVIVGAQKIIYANAVSETGD 467 Query: 475 PANTTSFDAYLASSRNESVPSSYKALVGGTAYNNFDTSADTGVSASDIDSVSTVEQIVTA 534 AN SFDAYLA S +E VPSSYK + GGT+Y+NFDT+ D GV + +++ V +VT+ Sbjct: 468 SANAASFDAYLAKSADEKVPSSYKTVAGGTSYDNFDTTKDLGVKSGSLNNAEDVPSVVTS 527 Query: 535 E--AGRMNGGDFTWAFNDSVDDASYALNSALMSAIRSY-TTQLVSVGG 579 AG + G W F D+ D+ Y++N L + + +Y T LVSVGG Sbjct: 528 SKGAGSLGRGVIDWKFTDA-DNDDYSVNKELKATVTNYRNTSLVSVGG 574 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.129 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,993,149,808 Number of extensions: 168318085 Number of successful extensions: 356892 Number of sequences better than 1.0e-05: 246 Number of HSP's gapped: 356595 Number of HSP's successfully gapped: 256 Length of query: 749 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 605 Effective length of database: 1,704,070,043 Effective search space: 1030962376015 Effective search space used: 1030962376015 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 135 (56.6 bits)