BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0004.p (461 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A0SGK5.1 A0SGK5_9BACL TetL 432 e-119 B0YMX0.1 B0YMX0_LACSK TetL 430 e-118 Q45683.1 Q45683_BACSU TET.BSR 422 e-116 P23054.1 TCRB_BACSU Tetracycline resistance protein 422 e-116 B5UXH3.1 B5UXH3_BACCE Tetracycline resistance protein 419 e-115 >A0SGK5.1 A0SGK5_9BACL TetL Length = 460 Score = 432 bits (1110), Expect = e-119, Method: Compositional matrix adjust. Identities = 211/443 (47%), Positives = 302/443 (68%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA F+ P++ NWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQILIWLCVLSFFSVLNEMVLNVSLPDIANDFNKSPASTNWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQGAGA+A PAL+MV VARYI Sbjct: 73 SDQLGIKRLLLFGIIINCFGSVIGFVGHSFFPILILARFIQGAGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EGIGPA+GG+IA +IHWSYL ++PMMT++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSIVAMGEGIGPAIGGMIAHFIHWSYLLLIPMMTIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 KG FD++G L+SVGIV FMLFT+ Y +L IS+L F F++ IR+ +PF+EP+L Sbjct: 193 VRIKGHFDIVGIILMSVGIVFFMLFTTSYNISFLIISILSFLIFVKHIRKVTEPFVEPAL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F +IS VPYMMKDV+ + T+ IGS ++FPG +SV++FG++ Sbjct: 253 GKNISFIIGVLCGGLIFGTVAGFISMVPYMMKDVHQLSTAAIGSVIIFPGAMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G+ L VSFL + F++ TPWL++ +V FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGPLYVLTIGVTFLSVSFLIAAFFLEVTPWLLTIILVFVFGGLSFTKTVISTI 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EGIG++ GGLL++ L L+P D + +LYSN+ Sbjct: 373 VSSSLKQKEAGAGMSLLNFTSFLSEGIGIAIVGGLLSVRLLNQKLLPMDLDQSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 + +F V+ +I Y R Q Sbjct: 433 LFLFTGVVVTSWLITLNVYKRRQ 455 >B0YMX0.1 B0YMX0_LACSK TetL Length = 460 Score = 430 bits (1106), Expect = e-118, Method: Compositional matrix adjust. Identities = 210/443 (47%), Positives = 301/443 (67%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA F+ P++ NWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQILIWLCVLSFFSVLNEMVLNVSLPDIANDFNKSPASTNWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD I V H +F ++++ARFIQGAGA+A PAL+MV VARYI Sbjct: 73 SDQLGIKRLLLFGIIINCFGSVIAFVGHSFFPILILARFIQGAGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EGIGPA+GG+IA +IHWSYL ++PMMT++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSIVAMGEGIGPAIGGMIAHFIHWSYLLLIPMMTIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 KG FD++G L+SVGIV FMLFT+ Y +L IS+L F F++ IR+ +PF+EP+L Sbjct: 193 VRIKGHFDIVGIILMSVGIVFFMLFTTSYNISFLIISILSFLIFVKHIRKVTEPFVEPAL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F +IS VPYMMKDVY + T+ IGS ++FPG +SV++FG++ Sbjct: 253 GKNISFIIGVLCGGLIFGTVAGFISMVPYMMKDVYQLSTAAIGSVIIFPGAMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G+ L VSFL + F++ TPWL++ +V FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGPLYVLTIGVTFLSVSFLIAAFFLEVTPWLLTIILVFVFGGLSFTKTVISTI 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG+G++ GGLL++ L L+P D + +LYSN+ Sbjct: 373 VSSSLKQKEAGAGMSLLNFTSFLSEGMGIAIVGGLLSVRLLNQKLLPMDLDQSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 + +F V+ +I Y R Q Sbjct: 433 LFLFTGVVVTSWLITLNVYKRRQ 455 >Q45683.1 Q45683_BACSU TET.BSR Length = 458 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/443 (44%), Positives = 302/443 (68%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA +F+ P++ANWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQG GA+A PAL+MV VARYI Sbjct: 73 SDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG++A YIHWSYL ++P T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSLVAMGEGVGPAIGGMVAHYIHWSYLLLIPTATIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 +G D+ G L+S GIV FMLFT+ Y++ +L IS+L FF F+Q IR+A+ PF++P L Sbjct: 193 ERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHIRKAQDPFVDPEL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G +L FL + F+D PW+++ ++ FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTV 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ +L L+P D + +LYSN+ Sbjct: 373 VSSSLKEKEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +++F +++ ++ Y R++ Sbjct: 433 LILFAGTIVICWLVILNVYKRSR 455 >P23054.1 TCRB_BACSU Tetracycline resistance protein Length = 458 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/443 (44%), Positives = 302/443 (68%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA +F+ P++ANWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQG GA+A PAL+MV VARYI Sbjct: 73 SDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG++A YIHWSYL ++P T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSLVAMGEGVGPAIGGMVAHYIHWSYLLLIPTATIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 +G D+ G L+S GIV FMLFT+ Y++ +L IS+L FF F+Q IR+A+ PF++P L Sbjct: 193 ERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHIRKAQDPFVDPEL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G +L FL + F+D PW+++ ++ FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTV 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ +L L+P D + +LYSN+ Sbjct: 373 VSSSLKEKEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +++F +++ ++ Y R++ Sbjct: 433 LILFAGIIVICWLVILNVYKRSR 455 >B5UXH3.1 B5UXH3_BACCE Tetracycline resistance protein Length = 458 Score = 419 bits (1076), Expect = e-115, Method: Compositional matrix adjust. Identities = 204/435 (46%), Positives = 295/435 (67%), Gaps = 3/435 (0%) Query: 2 TKSNSGSV---SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMIS 58 S+S S+ ++ ++WL +L+FFSV NE V NVSLPDIA F+ P++ NW+NT+F+++ Sbjct: 2 NSSHSHSILRHNQILLWLCVLSFFSVLNEMVLNVSLPDIASDFNKEPASINWINTAFILT 61 Query: 59 FAVGSALYGKISDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVP 118 F++G+A+YGK+SD IG V H +F V+++ARFIQGAGA+A P Sbjct: 62 FSIGTAVYGKLSDQLGIKKLLLFGIIVNCFGSVIGFVGHSFFSVLIMARFIQGAGAAAFP 121 Query: 119 ALIMVFVARYIEPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLT 178 AL+MV VARYI N GKAFG+IGS+VA EG+GP+VGGVIA+Y HWSY+ +LP++T++T Sbjct: 122 ALVMVVVARYIPKENSGKAFGLIGSIVAMGEGVGPSVGGVIAEYAHWSYILLLPVVTIIT 181 Query: 179 LPFFLHVLPDEPSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIR 238 +PF +L E KG DV G +SVGIV F++FT+ Y+ +L +S++CF F++ IR Sbjct: 182 VPFLAKLLKQEDIIKGSIDVKGIIYMSVGIVFFIMFTTSYRISFLVVSIICFLIFVKHIR 241 Query: 239 RAKQPFIEPSLLRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFP 298 + PF+ PSL + F +IS VPYMM+DVY + T+ IGS ++FP Sbjct: 242 KVSNPFVNPSLGKNISFMIGIICGGLILGTVAGFISMVPYMMRDVYQLSTAAIGSWIIFP 301 Query: 299 GTISVLLFGFVSGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGG 358 G +SV++FG++ G+LVDK+G F + G+ L +SFL +LF++ TPW+I+ ++ FGG Sbjct: 302 GAMSVIVFGYIGGVLVDKKGSLFVLTTGVAFLSISFLVAALFIETTPWVITIIVIFVFGG 361 Query: 359 LSFIKTVVSTSVAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTV 418 LSF KTV+ST V+ +L E+G+GMSFLNF FL+EG G++ GGLL++ L L+P Sbjct: 362 LSFTKTVISTIVSSSLEQKEAGAGMSFLNFTSFLSEGTGIAIVGGLLSIHLLNRKLLPIN 421 Query: 419 TDTAAFLYSNLMLVF 433 D + LYSNL+L+F Sbjct: 422 ADPSTHLYSNLLLIF 436 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,350,608,272 Number of extensions: 90780942 Number of successful extensions: 385802 Number of sequences better than 1.0e-05: 5184 Number of HSP's gapped: 383471 Number of HSP's successfully gapped: 5189 Length of query: 461 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 322 Effective length of database: 1,751,175,118 Effective search space: 563878387996 Effective search space used: 563878387996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)