BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0073.0004.p (591 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D596.1 B1D596_9BACL Histidine kinase internal region 910 0.0 B3KAH5.1 B3KAH5_9BACI Histidine kinase internal region 410 e-112 Q9KDT9.1 Q9KDT9_BACHD BH1122 protein 310 2e-82 Q5WL30.1 Q5WL30_BACSK Two-component sensor histidine kinase 285 1e-74 C3WSQ9.1 C3WSQ9_9FUSO Two-component sensor kinase yesM 193 5e-47 >B1D596.1 B1D596_9BACL Histidine kinase internal region Length = 584 Score = 910 bits (2351), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/585 (75%), Positives = 506/585 (86%), Gaps = 1/585 (0%) Query: 7 MRVNPFKTLRIDVVFFFGFALIVAILVGLIVWFSYINGSKEIAGNTSYYQQKLLIELHKK 66 MR+NPFKT+RI+ VFFFGFALIV ILV ++V FSY NGSKEIA NTSYYQQK+L+E+HKK Sbjct: 1 MRLNPFKTMRIEYVFFFGFALIVTILVAIVVSFSYYNGSKEIADNTSYYQQKVLVEIHKK 60 Query: 67 LNTNLEDIEQSSNTAAQNFNDQYEEMLDGNAYDQVRGQASIRSQLNNYVFGMPILQSIHV 126 L TNL+DIEQSSNTA+ NF + YEEM GN YDQVRGQA IR+QLNN+VFGMPI+ SIHV Sbjct: 61 LTTNLKDIEQSSNTASLNFTNMYEEMSGGNKYDQVRGQAGIRAQLNNFVFGMPIVHSIHV 120 Query: 127 YSDLTPSYNVQDFVQFLPIGQMEREAWYDDLRNSDYAWLSERTIKTNSGDTSVISFARKV 186 YSDLTPSYNVQ+FVQFLP+ +E+EAWY DL +SD AW+SER IKTN+GDT VIS+ARKV Sbjct: 121 YSDLTPSYNVQEFVQFLPLDGIEKEAWYKDLEHSDEAWVSERIIKTNNGDTPVISYARKV 180 Query: 187 YNRFNRAFAVMVFNVRVPAFQSLIATEDSRSNLALLDATDKLITSTGDEAFFQQHEAQIK 246 YNRFNRAFAVMVFNV+VP FQSLI TEDSRSNLALLDAT KLIT G FF H+A+IK Sbjct: 181 YNRFNRAFAVMVFNVKVPDFQSLITTEDSRSNLALLDATGKLITYIGKADFFTSHQAEIK 240 Query: 247 AQLDSRTNGAKRTGDEFLVWSSSLDSQWSLVEITSWSQITAGSLKQTKVILALGIATIVM 306 QL+ + +GAKRTGDEFLVW+SSLDS+WSL+E+TSW QITAGSLKQT+ ILA GI TI++ Sbjct: 241 EQLEQKNSGAKRTGDEFLVWTSSLDSKWSLLEVTSWRQITAGSLKQTRTILAFGIVTIIL 300 Query: 307 ILLLAVFLSRQFVKPIGLVLKAMSNFSLSKRTPLPDDYKNEFGRLFQGYEKLTYRIEELY 366 ILLLAVFLSRQF+KPIGL+LKAMSNFSL+KR PLP DYKNEFG LFQGY+KL RIE+LY Sbjct: 301 ILLLAVFLSRQFIKPIGLLLKAMSNFSLNKRVPLPADYKNEFGTLFQGYDKLLSRIEQLY 360 Query: 367 DSLEQQHEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEASQPQISSMLSHLGQFFRLA 426 +LE++HEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEA QPQISSMLSHLGQFFRL+ Sbjct: 361 GTLEEKHEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEADQPQISSMLSHLGQFFRLS 420 Query: 427 LSNSDSLVPLSEELAHIESYLQFQKIRWEDRLEYRFIAADEVKSLFVPKIILQPFVENAF 486 L+N++SLVPL+EEL HIESYLQFQKIRWEDRL Y +E L+VPK+ILQPFVENAF Sbjct: 421 LTNTNSLVPLTEELTHIESYLQFQKIRWEDRLTYTLNVPEEAGKLYVPKVILQPFVENAF 480 Query: 487 IHGFHGKRQASLIITVSILEGQMLQITVMDNGRQLQENWMEKRSAKGGYGLRNVRERITA 546 +HGFHGK+QASL I VS+ Q+L I + D+GR+L+E+W EKRSAKGGYGLRNVRERI A Sbjct: 481 VHGFHGKQQASLDIVVSVGFDQLL-IVIRDDGRKLREDWQEKRSAKGGYGLRNVRERIEA 539 Query: 547 MFGSTYGFTLGNSPEGGTQAVICLPVLYSPSVKGGTQHVESSHHR 591 +FG+ YGFTL NSPEGGT+A I LP L + KG HVE+S HR Sbjct: 540 LFGNKYGFTLSNSPEGGTEASIRLPRLETLKQKGERLHVENSDHR 584 >B3KAH5.1 B3KAH5_9BACI Histidine kinase internal region Length = 574 Score = 410 bits (1054), Expect = e-112, Method: Compositional matrix adjust. Identities = 224/572 (39%), Positives = 346/572 (60%), Gaps = 11/572 (1%) Query: 9 VNPFKTLRIDVVFFFGFALIVAILVGLIVWFSYINGSKEIAGNTSYYQQKLLIELHKKLN 68 +NPFK RID +F FA ++ I++ + VW SY+ S+E+A NTSYYQ++LL EL+ ++ Sbjct: 2 INPFKKYRIDSLFIICFAGLITIVLAITVWTSYVMTSRELAENTSYYQKRLLEELNNEIT 61 Query: 69 TNLEDIEQSSNTAAQNFNDQYEEMLDG--NAYDQVRGQASIRSQLNNYVFGMPILQSIHV 126 L IEQ S T +++ ND L G + ++ R I+ L + + +P +Q+I + Sbjct: 62 GRLTSIEQISLTTSRD-NDLLT-FLSGKEDEFNHYRRSKGIQEALAHLTYSIPQIQAIDL 119 Query: 127 YSDLTPSYNVQDFVQFLPIGQMEREAWYDDLRNSDYAWLSERTIKTNSGDTSVISFARKV 186 Y + + Q ++QF + + E + WY L+N+D++W E + T +G+ V+SF+RKV Sbjct: 120 YMEQPEWGDRQSYIQFHGLTEAETKPWYPLLQNNDFSWSEEHDLTTYNGNVPVLSFSRKV 179 Query: 187 YNRFNRAFAVMVFNVRVPAFQSLIATEDSRSNLALLDATDKLITSTGD---EAFFQQHEA 243 YN +R ++V +V+ + ++ SN LLD + + S G +A Q+ + Sbjct: 180 YNN-SRYLGILVVHVKADWIRGILEGYSKDSNRMLLDLNGRELLSVGKSMRDAGLQEQDL 238 Query: 244 QIKAQLD-SRTNGAKRTGDEFLVWSSSLDSQWSLVEITSWSQITAGSLKQTKVILALGIA 302 + Q SR + + +V+S DS W LVEIT W +IT+GS+K I+ +GI Sbjct: 239 RFADQSGVSRLHTQPHGVESLIVYSKVADSNWMLVEITPWERITSGSVKLAGAIITIGIG 298 Query: 303 TIVMILLLAVFLSRQFVKPIGLVLKAMSNFSLS-KRTPLPDDYKNEFGRLFQGYEKLTYR 361 +++ LL ++LSRQ +KPI +++AM N+ + K+ L DY NEFG LF G+ +L R Sbjct: 299 ALLLAFLLTLWLSRQLLKPIAQLVRAMKNYDVGGKQAELAGDYTNEFGILFAGFRRLNER 358 Query: 362 IEELYDSLEQQHEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEASQPQISSMLSHLGQ 421 I+ LY SL Q++EQQR AEI+ALQ INPHFLYN+LDQ+NWMAI Q ++S +L +G+ Sbjct: 359 IQALYASLAQRYEQQRKAEIEALQANINPHFLYNTLDQLNWMAIAEGQEKMSRILELMGR 418 Query: 422 FFRLALSNSDSLVPLSEELAHIESYLQFQKIRWEDRLEYRFIAADEVKSLFVPKIILQPF 481 FR+ LS+ +S + + EEL H+ YL+ Q+IRWED LEY A+ E+++LF+PK+ LQPF Sbjct: 419 MFRIGLSHGESFITIEEELTHVACYLEIQQIRWEDGLEYEVHASQEIRNLFIPKMTLQPF 478 Query: 482 VENAFIHGFHGKRQASLIITVSILEGQMLQITVMDNGRQLQENWMEKRSAKGGYGLRNVR 541 VEN+ IHGF+ ++ + I EG +++T++D+G L KGGYG+RNVR Sbjct: 479 VENSIIHGFNSRQNGRIHIKAE-EEGGRIRLTIIDDGSGLAPLSKGNHRRKGGYGIRNVR 537 Query: 542 ERITAMFGSTYGFTLGNSPEGGTQAVICLPVL 573 ER +A F Y + + +GG I P L Sbjct: 538 ERFSAFFNDDYEIIVKDGEKGGVVVSISFPRL 569 >Q9KDT9.1 Q9KDT9_BACHD BH1122 protein Length = 586 Score = 310 bits (794), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 187/572 (32%), Positives = 301/572 (52%), Gaps = 19/572 (3%) Query: 12 FKTLRIDVVFFFGFALIVAILVGLIVWFSYINGSKEIAGNTSYYQQKLLIELHKKLNTNL 71 FK +I +FF F + + +I+ SY E+ T+ YQ++LL L ++ L Sbjct: 4 FKRYQIRHIFFTSFFAFMLLAFIVIISVSYRYSVNEMVQATTEYQEQLLTLLSNDISNYL 63 Query: 72 EDIEQSSNTAAQ--NFNDQYEEMLDGNAYDQVRGQASIRSQLNNYVFGMPILQSIHVYSD 129 + + S ++ NF + D Y+Q+ S+ L+N V+ +P L SI VY Sbjct: 64 QSYDDISVGLSRQSNFRQALKGSEDPYTYNQLF--QSLSRDLSNIVYSVPGLHSIEVYLH 121 Query: 130 LTPSYNVQDFVQFLPIGQMEREAWYDDLRNSDYAWLSERTIKTNSGDTSVISFARKVYNR 189 P +Q + +LP+ +E W L + W+ ER T +G+ V+S AR V+ Sbjct: 122 SPPHSEIQQPINYLPLEAVEEMGWTKRLDGKNSVWIGERVSYTMAGEERVLSLARNVFQA 181 Query: 190 FNRAFAVMVFNVRVPAFQSLIATEDSRSNLALLDATDKLITSTGDE-------AFFQQHE 242 V+V NV ++ + + SNL ++D + + ++ + +F Q+ Sbjct: 182 RGEIEGVLVLNVHASTIETWLKQKSQESNLMIIDKQGRFVATSDPQHESVLYSPYFDQY- 240 Query: 243 AQIKAQLDSRTNGAKRTGDEFLVWSSSLDSQWSLVEITSWSQITAGSLKQTKVILALGIA 302 + Q +T + D V +++W+L ++T +S++T GS + +++ +G Sbjct: 241 --LPYQPTMQTGENDKDEDALEVSMPIPNTEWTLKQVTPYSELTEGSRRMAAILVIIGAI 298 Query: 303 TIVMILLLAVFLSRQFVKPIGLVLKAMSNFSLSKRT-PLPDDYKNEFGRLFQGYEKLTYR 361 IV+ LL+ L+++F PI + +S + + LP DY+NEFG LF+GY+ L R Sbjct: 299 MIVVALLVTYSLTKKFTTPIYELKNVLSKYPDQDLSGELPSDYRNEFGELFEGYKGLIRR 358 Query: 362 IEELYDSLEQQHEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEASQPQISSMLSHLGQ 421 ELY SL +Q+++QR AEIKALQ INPHFLYN+LDQ+NWMAIE +S ML +LGQ Sbjct: 359 NNELYRSLHRQYKRQREAEIKALQANINPHFLYNTLDQLNWMAIEKGDRTMSHMLENLGQ 418 Query: 422 FFRLALSNSDSLVPLSEELAHIESYLQFQKIRWEDRLEYRFIAADEVKSLFVPKIILQPF 481 R+ LSN +S++PL +E+ +++ YL+ QK++ +RL Y + +PK+ LQPF Sbjct: 419 MLRIGLSNGESILPLEKEVEYLKYYLKIQKLKMGERLTYTVDVPVMLYRYLIPKLTLQPF 478 Query: 482 VENAFIHGFHGKRQASLIITVSILEGQMLQITVMDNGRQLQENWMEKRSA--KGGYGLRN 539 VEN FIHGF R ++I ++ + + +T+ DNG Q KRS GGYG+RN Sbjct: 479 VENCFIHGFQDGRDGEVVIR-AMEDQNHIVMTIQDNGIGFQP-VTTKRSKLDMGGYGIRN 536 Query: 540 VRERITAMFGSTYGFTLGNSPEGGTQAVICLP 571 V ER+ F + +GGT I +P Sbjct: 537 VMERLDVYFDRYASVHVTGDEQGGTLVKIIIP 568 >Q5WL30.1 Q5WL30_BACSK Two-component sensor histidine kinase Length = 581 Score = 285 bits (728), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 182/587 (31%), Positives = 310/587 (52%), Gaps = 20/587 (3%) Query: 12 FKTLRIDVVFFFGFALIVAILVGLIVWFSYINGSKEIAGNTSYYQQKLLIELHKKLNTNL 71 FK ++ + F AL + L +++ SY E +TS +Q++LL +L+ + Sbjct: 2 FKKAKVKTILFLSIALFIIFLFFVVIGVSYRMVVSETIASTSAHQKELLHLYRNELDNQM 61 Query: 72 EDIEQSSNTAAQN------FNDQYEEMLDGNAYDQVRGQASIRSQLNNYVFGMPILQSIH 125 + +E+++ ++N N+++E +Y++ R ++ L+ V ++ SI Sbjct: 62 KVLEETAVVISRNADLQAFINNRHEHF----SYNRQRN--NLHKYLHQVVLSSSMIDSID 115 Query: 126 VYSDLTPSYNVQDFVQFLPIGQMEREAWYDDLRNSDYAWLSERTIKTNSGDTSVISFARK 185 +Y D P+++ Q ++FL M ++ ++ WL E K + + V+S+ K Sbjct: 116 IYMDNPPTFDSQYPIRFLSKIDMHSHPKNKEVNEANEVWLGEHD-KHLTEEEKVVSYMCK 174 Query: 186 VYNRFNRAFAVMVFNVRVPAFQSLIATEDSRSNLALLDATDKLITSTGDEAFFQQHEAQI 245 +Y+ A++V NVR F++L D +N +L + E + +I Sbjct: 175 LYSAKGTLKAMLVLNVRTRDFEALFVDRDHHANQRMLVGKNGEAIMAARET--DEEVGRI 232 Query: 246 KAQLDSRTNGAKRTGDEFL-VWSSSLDSQWSLVEITSWSQITAGSLKQTKVILALGIATI 304 A+ S G KR D+ L VWS+ + W LVE+T W ++T G + T V++A+ + I Sbjct: 233 VAE-HSFNQGGKRELDQGLAVWSALERADWFLVEVTPWGELTKGGKRITAVLIAISLVAI 291 Query: 305 VMILLLAVFLSRQFVKPIGLVLKAMSNFSLSKRTPLPDDYKNEFGRLFQGYEKLTYRIEE 364 LL A+ LS F KPI + + F S++ +P Y+NEFG LF+G+ ++T I Sbjct: 292 AGALLFALLLSTAFSKPILQLRQGFVQFGKSQKVNVPIGYQNEFGDLFEGFTEMTKEIHA 351 Query: 365 LYDSLEQQHEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEASQPQISSMLSHLGQFFR 424 L+ SLE++H ++ A+I ALQ INPHFLYN+LDQ+NWMAI Q +IS ++ +G+ R Sbjct: 352 LHQSLEEEHRRKNEADITALQANINPHFLYNTLDQLNWMAIAKGQDEISEVIELMGKMLR 411 Query: 425 LALSNSDSLVPLSEELAHIESYLQFQKIRWEDRLEYRFIAADEVKSLFVPKIILQPFVEN 484 + LS S +P+++EL HI Y+Q Q+ + + E+ +VPK LQP VEN Sbjct: 412 IGLSKGKSFIPIADELDHIRYYMQIQQCTKSVDISFEIDVPSELHHYYVPKFTLQPIVEN 471 Query: 485 AFIHGFHGKRQASLIITVSILEGQMLQITVMDNGRQLQENWMEKRSAKGGYGLRNVRERI 544 A +HGFH +++ + I++ + Q LQ+ V D+G + E K GYGL+NV ER+ Sbjct: 472 AIVHGFHRRQRGMIDISIRPYK-QALQLQVKDDGVGFDQK--EPIPRKNGYGLQNVAERL 528 Query: 545 TAMFGSTYGFTLGNSPEGGTQAVICLPVLYSPSVKGGTQHVESSHHR 591 A FG T+ ++ GT +I +P + G ++VE+ ++R Sbjct: 529 RAFFGEEGTITVYSAIGAGTTVLIEIPQKQTSDWGGVNKYVENGYYR 575 >C3WSQ9.1 C3WSQ9_9FUSO Two-component sensor kinase yesM Length = 552 Score = 193 bits (490), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 160/587 (27%), Positives = 280/587 (47%), Gaps = 59/587 (10%) Query: 7 MRVNPFKTLRIDVVFFFGFA-LIVAILVGLIVWFSYINGSKEIAGNTSYYQQKLLIELHK 65 M++N K L I + F+F L++ +L+G I +FS LLI K Sbjct: 1 MKMNN-KPLNIKIGFYFLITNLVLVLLLGSIFYFS---------------SSSLLI--RK 42 Query: 66 KLNTNLEDIEQSSNTAAQNFNDQYEEML---------DGNAYDQVRGQAS-----IRSQL 111 +++ E IE+S N + + Y L D YD ++ + I S + Sbjct: 43 EISAKTEAIEKSGN-----YIELYMSKLTTLSQVISHDKGVYDYLKNKDESEKNRILSII 97 Query: 112 NNYVFGMPILQSIHVYSDLTPSYNVQDFVQFLPIGQMEREAWY-DDLRNSDYAW--LSER 168 +N + P ++SI + + + V M +E WY + L N L ++ Sbjct: 98 DNTLSTDPYIKSIILIRKDGAVISNEKNVNMEVSSDMMKEEWYVNSLMNPMPVLNPLRKQ 157 Query: 169 TIKTNSGDTSVISFARKVYNRFNRAFAVMVFNVRVPAFQSLIATEDS--RSNLALLDATD 226 + D VIS +R++ + V++ +V+ A + +++ S++ +LD + Sbjct: 158 NFSLDGMDDWVISVSREIADTNGENLGVLLIDVKYQALHEYLQNQETGKNSDIVILDEDN 217 Query: 227 KLITSTGDEAFFQQHEAQIKAQLDSRTNGAKRTGDEFLVWSSSLDSQWSLVEITSWSQIT 286 +++ E + + + L + G R + V ++ W+L+EI+ +I Sbjct: 218 RIVYYK--EIPYDTSQEKYLKNLKNIEEGYNRKENTVTVKYPIKNTHWTLIEISYMQEIE 275 Query: 287 AGSLKQTKVILALGIATIVMILLLAVFLSRQFVKPIGLVLKAMSNFS--LSKRTPLPDDY 344 + ++I+ +A++++ +L+++ + R+ KPI + + M+NF+ LSK L D Sbjct: 276 SLKNHFFEMIVISCLASLLITVLISISVLRRITKPIKELEQHMNNFNNDLSK-INLKGDV 334 Query: 345 KNEFGRLFQGYEKLTYRIEELYDSLEQQHEQQRAAEIKALQMMINPHFLYNSLDQVNWMA 404 E L + ++ RI+ L R EI AL INPHFLYN+LD + WMA Sbjct: 335 SIEILSLQNHFNEMIDRIKYL-----------REYEINALYSQINPHFLYNTLDTIIWMA 383 Query: 405 IEASQPQISSMLSHLGQFFRLALSNSDSLVPLSEELAHIESYLQFQKIRWEDRLEYRFIA 464 ++ S+ L FFR++LSN +PL EE+ HI+ YL QK R+ED+LEY+ Sbjct: 384 EFQDTEKVISITKALSNFFRISLSNGKEKIPLKEEINHIKEYLYIQKQRYEDKLEYKISI 443 Query: 465 ADEVKSLFVPKIILQPFVENAFIHGFHGKRQASLIITVSILEGQMLQITVMDNGRQLQEN 524 +E++++ VPKIILQPFVENA HG +I S + +++ + DNG + Sbjct: 444 QEELENIEVPKIILQPFVENAIYHGIKNLDTTGIISIYSQIIENKIELIIEDNGIGFEAA 503 Query: 525 WMEKRSAKGGYGLRNVRERITAMFGSTYGFTLGNSPEGGTQAVICLP 571 + GG G++NV +RI +G+ YG + +S + G + +I LP Sbjct: 504 KKQALMKMGGVGIKNVNKRIQYYYGNEYGAKIDSSFKTGARIIITLP 550 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,426,909,141 Number of extensions: 138410390 Number of successful extensions: 414244 Number of sequences better than 1.0e-05: 2035 Number of HSP's gapped: 411111 Number of HSP's successfully gapped: 2035 Length of query: 591 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 449 Effective length of database: 1,722,912,073 Effective search space: 773587520777 Effective search space used: 773587520777 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)