BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0007.p (546 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z8J5.1 C0Z8J5_BREBN Probable formate--tetrahydrofolate ligase ... 654 0.0 A3DI22.1 FTHS_CLOTH Formate--tetrahydrofolate ligase EC=6.3.4.3 593 e-167 B4BAJ3.1 B4BAJ3_CLOTM Formate--tetrahydrofolate ligase EC=6.3.4.3 591 e-167 B8I3S9.1 FTHS_CLOCE Formate--tetrahydrofolate ligase EC=6.3.4.3 588 e-166 B7APZ7.1 B7APZ7_9BACE Putative uncharacterized protein 581 e-164 >C0Z8J5.1 C0Z8J5_BREBN Probable formate--tetrahydrofolate ligase EC=6.3.4.3 Length = 556 Score = 654 bits (1688), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/545 (58%), Positives = 402/545 (73%), Gaps = 4/545 (0%) Query: 1 MRLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKT 60 M+ I E+A +AGI E+HLELYGKYK+KLS LW+++K DGKL+LVTAMNP PAG GKT Sbjct: 15 MKSIVEIAKSAGIEEQHLELYGKYKAKLSDELWEQVKDRPDGKLILVTAMNPNPAGAGKT 74 Query: 61 LTTIGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 LTTIGLSQALN G KT+AALREPSL QI+PADEINLHFTGDIHA Sbjct: 75 LTTIGLSQALNHLGKKTMAALREPSLGPCMGIKGGATGSGQAQILPADEINLHFTGDIHA 134 Query: 121 VTSAHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLGDGNGTVRESG 180 +T+AHNLL+AMIDNH+F GN GL+P++IVWKR MDMNDR+LR+IV GLG+GNG RE G Sbjct: 135 ITAAHNLLAAMIDNHIFHGNPRGLNPKKIVWKRAMDMNDRALRNIVVGLGEGNGMTREDG 194 Query: 181 FQITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAVK 240 F ITTASEIMA+LCL + LADL++RL+R+++GYD + QPV A++I AV+AM LLKEA+K Sbjct: 195 FMITTASEIMAILCLSENLADLRERLNRIIIGYDFNDQPVRAEEINAVDAMCVLLKEAIK 254 Query: 241 PNLVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMDI 300 PNLVQT+EGTPVI+HGGPFANIAHGCSSVIGT+ ALKL D VVTEAGFGADLGAEKF DI Sbjct: 255 PNLVQTIEGTPVIIHGGPFANIAHGCSSVIGTKMALKLADYVVTEAGFGADLGAEKFFDI 314 Query: 301 KCRQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGVP 360 KCR+ +SLKYNGGV+ +L N +AL G N++RH+ENL KFGVP Sbjct: 315 KCRKAGLTPSAAVVVITVRSLKYNGGVKVPDLAVENLSALEKGFENVKRHLENLQKFGVP 374 Query: 361 VLVALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEGQ 420 +VA+NHF D +EI+ V+ C+ G AA+S+VW + Sbjct: 375 AVVAINHFATDTQAEIDAVIALCQAAGAEAALSKVW----AEGGAGGVELAEKVIRAAEL 430 Query: 421 PAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKTP 480 P++F LYE++L I KI+ +V E+YRG+ V FSP A +++ +E++G+ L VCMAKTP Sbjct: 431 PSSFRFLYEEDLSIKDKIETVVKEVYRGSGVVFSPLALKTMQKVEEMGMSHLPVCMAKTP 490 Query: 481 YSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLDG 540 YSF+D+ LLGAP FT+ V ++ +S GAGF V +TG++VTMPGLP +PAAE + VD DG Sbjct: 491 YSFTDRADLLGAPVDFTIHVSEVRISAGAGFIVALTGSLVTMPGLPKKPAAESLFVDEDG 550 Query: 541 VINGL 545 + GL Sbjct: 551 HVIGL 555 >A3DI22.1 FTHS_CLOTH Formate--tetrahydrofolate ligase EC=6.3.4.3 Length = 556 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 304/546 (55%), Positives = 372/546 (68%), Gaps = 4/546 (0%) Query: 1 MRLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKT 60 M+ IT+VA+ GI EE LELYGKYK+KLS LW+ +K DGKLVLVTA+NPTPAGEGKT Sbjct: 13 MKPITQVAAELGIDEEELELYGKYKAKLSDKLWERVKDRPDGKLVLVTAINPTPAGEGKT 72 Query: 61 LTTIGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 TT+GL QA+ G K V ALREPSL Q+VP ++INLHFTGD+HA Sbjct: 73 TTTVGLGQAMARIGKKAVIALREPSLGPVMGIKGGAAGGGYSQVVPMEDINLHFTGDMHA 132 Query: 121 VTSAHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLG-DGNGTVRES 179 +T+A+NLLSA IDNH+ QGN L +D ++I+WKR MDMNDR+LR+IV GLG NG RE Sbjct: 133 ITAANNLLSAAIDNHIQQGNELNIDVRQIIWKRAMDMNDRALRNIVVGLGGKANGVPRED 192 Query: 180 GFQITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAV 239 GFQIT ASE+MAVLCL GL DLK+RL R+L+GY G+PV AKD+ AM LLK+A+ Sbjct: 193 GFQITVASEVMAVLCLSTGLMDLKERLGRILIGYTYDGKPVFAKDLKVNGAMALLLKDAI 252 Query: 240 KPNLVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMD 299 KPNLVQTLE TP IVHGGPFANIAHGC+S++ TR LKL D +TEAGFGADLGAEKF + Sbjct: 253 KPNLVQTLENTPAIVHGGPFANIAHGCNSIVATRLGLKLADYCITEAGFGADLGAEKFFN 312 Query: 300 IKCRQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGV 359 IKCR ++LKYNGGV+K+ L N AL G N+E+HIEN+ KF V Sbjct: 313 IKCRYAGLKPDLVVLVATIRALKYNGGVKKENLGIENLPALEKGFVNLEKHIENIRKFQV 372 Query: 360 PVLVALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEG 419 P+LVA+NHF+ D+ +EI V C+ L V A S V+ E Sbjct: 373 PLLVAINHFDTDSEAEIEYVKNRCKALNVEVAFSDVFSKGSEGGIELAEKVVKLT---ET 429 Query: 420 QPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKT 479 Q + F PLY+ NL I KI+ I EIY V PAA+R++ IE+L + +L +C+AKT Sbjct: 430 QKSNFKPLYDVNLSIREKIEIIAREIYGADSVNILPAAERAIKKIEELKMDKLPICVAKT 489 Query: 480 PYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLD 539 YS SD P LLG P+GF + VR+I LS GAGF V ITG+I+TMPGLP PAAE I +D + Sbjct: 490 QYSLSDDPTLLGRPQGFVITVREIKLSSGAGFIVAITGDIMTMPGLPKVPAAEKIDIDEN 549 Query: 540 GVINGL 545 GVI GL Sbjct: 550 GVITGL 555 >B4BAJ3.1 B4BAJ3_CLOTM Formate--tetrahydrofolate ligase EC=6.3.4.3 Length = 556 Score = 591 bits (1523), Expect = e-167, Method: Compositional matrix adjust. Identities = 304/546 (55%), Positives = 371/546 (67%), Gaps = 4/546 (0%) Query: 1 MRLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKT 60 M+ IT+VA+ GI EE LELYGKYK+KLS LW+ +K DGKLVLVTA+NPTPAGEGKT Sbjct: 13 MKPITQVAAELGIDEEELELYGKYKAKLSDKLWERVKDRPDGKLVLVTAINPTPAGEGKT 72 Query: 61 LTTIGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 TT+GL QA+ G K V ALREPSL Q+VP ++INLHFTGD+HA Sbjct: 73 TTTVGLGQAMARIGKKAVIALREPSLGPVMGIKGGAAGGGYSQVVPMEDINLHFTGDMHA 132 Query: 121 VTSAHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLG-DGNGTVRES 179 +T+A+NLLSA IDNH+ QGN L +D ++I+WKR MDMNDR+LR+IV GLG NG RE Sbjct: 133 ITAANNLLSAAIDNHIQQGNELNIDVRQIIWKRAMDMNDRALRNIVVGLGGKANGVPRED 192 Query: 180 GFQITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAV 239 GFQIT ASE+MAVLCL GL DLK+RL R+L+GY G+PV AKD+ AM LLK+A+ Sbjct: 193 GFQITVASEVMAVLCLSTGLMDLKERLGRILIGYTYDGKPVFAKDLKVNGAMALLLKDAI 252 Query: 240 KPNLVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMD 299 KPNLVQTLE TP IVHGGPFANIAHGC+S++ TR LKL D +TEAGFGADLGAEKF + Sbjct: 253 KPNLVQTLENTPAIVHGGPFANIAHGCNSIVATRLGLKLADYCITEAGFGADLGAEKFFN 312 Query: 300 IKCRQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGV 359 IKCR ++LKYNGGV+K+ L N AL G N+E+HIEN+ KF V Sbjct: 313 IKCRYAGLKPDLVVLVATIRALKYNGGVKKENLGIENLPALEKGFVNLEKHIENIRKFQV 372 Query: 360 PVLVALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEG 419 P+LVA+NHF D+ +EI V C+ L V A S V+ E Sbjct: 373 PLLVAINHFGTDSEAEIEYVKNRCKALNVEVAFSDVFSKGSEGGIELAEKVVKLT---ET 429 Query: 420 QPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKT 479 Q + F PLY+ NL I KI+ I EIY V PAA+R++ IE+L + +L +C+AKT Sbjct: 430 QKSNFKPLYDVNLSIREKIEIIAREIYGADSVNILPAAERAIKKIEELKMDKLPICVAKT 489 Query: 480 PYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLD 539 YS SD P LLG P+GF + VR+I LS GAGF V ITG+I+TMPGLP PAAE I +D + Sbjct: 490 QYSLSDDPTLLGRPQGFVITVREIKLSSGAGFIVAITGDIMTMPGLPKVPAAEKIDIDEN 549 Query: 540 GVINGL 545 GVI GL Sbjct: 550 GVITGL 555 >B8I3S9.1 FTHS_CLOCE Formate--tetrahydrofolate ligase EC=6.3.4.3 Length = 556 Score = 588 bits (1516), Expect = e-166, Method: Compositional matrix adjust. Identities = 292/546 (53%), Positives = 378/546 (69%), Gaps = 4/546 (0%) Query: 1 MRLITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKT 60 M I ++A GI E +E YG YK+KLS LWD++K+ +DGKLVLVTA+NPTPAGEGKT Sbjct: 13 MHHIADIAKNLGIDTEDIEFYGNYKAKLSDKLWDKVKNKKDGKLVLVTAINPTPAGEGKT 72 Query: 61 LTTIGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHA 120 TT+GL QA+ G V ALREPS+ Q+VP ++INLHFTGD+HA Sbjct: 73 TTTVGLGQAMARIGKNAVIALREPSMGPVMGIKGGAAGGGYAQVVPMEDINLHFTGDMHA 132 Query: 121 VTSAHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLGDG-NGTVRES 179 +T+A+NLLSA IDNHL QGN+L +D ++IVWKR MDMNDR+LR+++ GLG NG RE Sbjct: 133 ITAANNLLSAAIDNHLQQGNMLNIDSRQIVWKRCMDMNDRALRNVIVGLGGKINGVPRED 192 Query: 180 GFQITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAV 239 GF IT ASEIMA+LCL + DLKKRL R+++GY G+PVTA D+ AMT LLK+A+ Sbjct: 193 GFNITVASEIMAILCLALDIKDLKKRLGRIIIGYTYEGKPVTAHDLKVDGAMTLLLKDAI 252 Query: 240 KPNLVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMD 299 KPNLVQTLEGTP ++HGGPFANIAHGC+S+ T+ ALKL D V+TEAGFGADLGAEKF D Sbjct: 253 KPNLVQTLEGTPALMHGGPFANIAHGCNSISATKLALKLSDYVITEAGFGADLGAEKFFD 312 Query: 300 IKCRQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGV 359 IKCR ++LKYNGGVRK++L + N AL G +N E+HIENL +FGV Sbjct: 313 IKCRFAGFKPDAVVLVATIRALKYNGGVRKEDLKEENIDALSKGFANAEKHIENLKQFGV 372 Query: 360 PVLVALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEG 419 PV+VA+NHF+ D +EI + E C LGV A S V+ + Sbjct: 373 PVMVAINHFDTDTEAEIKLIQEKCSSLGVEVAFSDVFLKGGEGGIELAEKLVALT---DS 429 Query: 420 QPAAFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKT 479 + FAP+Y++ L I K+++IV++IY G V ++ AA++S+ IE++GL +L +CMAKT Sbjct: 430 TVSNFAPIYDEKLPIKEKVQQIVSKIYGGRNVIYNAAAEKSIAKIEEMGLDRLPICMAKT 489 Query: 480 PYSFSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLD 539 YS SD P LLG P+ F + V+++ +S GAGF VV+TG+I+TMPGLP PAAE I ++ Sbjct: 490 QYSLSDNPALLGRPQDFDVTVKEVRISAGAGFLVVLTGDIMTMPGLPKVPAAERIDINES 549 Query: 540 GVINGL 545 GVI GL Sbjct: 550 GVITGL 555 >B7APZ7.1 B7APZ7_9BACE Putative uncharacterized protein Length = 557 Score = 581 bits (1497), Expect = e-164, Method: Compositional matrix adjust. Identities = 290/544 (53%), Positives = 368/544 (67%), Gaps = 5/544 (0%) Query: 4 ITEVASAAGIHEEHLELYGKYKSKLSPSLWDELKHNQDGKLVLVTAMNPTPAGEGKTLTT 63 I EVA+ G+ E+ LELYGKYK+K+S D+ K N+D KLVLVTA+NPTPAGEGKT + Sbjct: 16 IKEVAAKIGMTEDDLELYGKYKAKISDDFIDKCKDNKDAKLVLVTAINPTPAGEGKTTVS 75 Query: 64 IGLSQALNAAGVKTVAALREPSLXXXXXXXXXXXXXXXXQIVPADEINLHFTGDIHAVTS 123 IGL+Q L G K + ALREPSL Q+VP +++NLHFTGD HA+TS Sbjct: 76 IGLTQGLCRLGRKAIVALREPSLGPCFGIKGGAAGGGYSQVVPMEDLNLHFTGDFHAITS 135 Query: 124 AHNLLSAMIDNHLFQGNLLGLDPQRIVWKRVMDMNDRSLRSIVTGLG-DGNGTVRESGFQ 182 A+NLL+AM+DNH+ QGN L +DP+RIVWKR +DMNDR+LR+IV G+G +G VRE F Sbjct: 136 ANNLLAAMLDNHMHQGNALHIDPKRIVWKRCIDMNDRALRNIVIGMGARADGVVREDHFI 195 Query: 183 ITTASEIMAVLCLCDGLADLKKRLSRMLVGYDQSGQPVTAKDIGAVEAMTALLKEAVKPN 242 IT ASEIMA+LCL D +ADLK RL +++V YD PVTA D+ A AM ALLK+A+KPN Sbjct: 196 ITVASEIMAILCLADDMADLKDRLGKIIVAYDYDNNPVTADDLHATGAMAALLKDAIKPN 255 Query: 243 LVQTLEGTPVIVHGGPFANIAHGCSSVIGTRYALKLGDVVVTEAGFGADLGAEKFMDIKC 302 LVQTLE TP IVHGGPFANIAHGC+SV T+ A+K+ D+VVTEAGFGADLGAEKF+DIKC Sbjct: 256 LVQTLENTPAIVHGGPFANIAHGCNSVRATKTAMKMADIVVTEAGFGADLGAEKFLDIKC 315 Query: 303 RQXXXXXXXXXXXXXXKSLKYNGGVRKDELHQTNQAALLSGISNMERHIENLGKFGVPVL 362 R ++LKYNGGV KD+L + N AL GI+N+++HIEN+ K+ VPV+ Sbjct: 316 RLAGLKPDAIVIVATVRALKYNGGVPKDQLSEENMEALRKGIANLDKHIENMHKYNVPVV 375 Query: 363 VALNHFEGDAPSEINDVLEACRRLGVPAAVSRVWXXXXXXXXXXXXXXXXXXXQGEGQPA 422 V LN F D E + + + C LG A+S+VW E +P+ Sbjct: 376 VTLNSFITDTEEEYDFIRKHCEELGCEFALSKVWAEGGRGGEELAQKVLSTL---ENKPS 432 Query: 423 AFAPLYEDNLDIPAKIKKIVTEIYRGAEVAFSPAAKRSLGMIEKLGLHQLQVCMAKTPYS 482 + PLYED L + KI+K+ EIY V++S AAK+ L I +LG VCMAKT YS Sbjct: 433 EYKPLYEDELSLEEKIEKVAKEIYGAGSVSYSAAAKKQLARITELGFGSFPVCMAKTQYS 492 Query: 483 FSDQPRLLGAPEGFTMGVRDITLSLGAGFAVVITGNIVTMPGLPAQPAAEGIQVDLD-GV 541 SD RLLG PEGF + VR++ ++ GAGF VV+ G ++TMPGLP PAAE + D++ G Sbjct: 493 LSDNARLLGRPEGFEVNVREVYVNAGAGFVVVLLGTVLTMPGLPRVPAAENVDYDIEAGR 552 Query: 542 INGL 545 I GL Sbjct: 553 ITGL 556 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,965,661,740 Number of extensions: 118889103 Number of successful extensions: 338723 Number of sequences better than 1.0e-05: 1642 Number of HSP's gapped: 332980 Number of HSP's successfully gapped: 1866 Length of query: 546 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 405 Effective length of database: 1,732,333,088 Effective search space: 701594900640 Effective search space used: 701594900640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)