BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0069.0001.p (214 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7N4.1 B3K7N4_9BACI MOSC domain containing protein 257 5e-67 O34542.1 YFLK_BACSU Uncharacterized protein yflK 206 8e-52 C0ZDC7.1 C0ZDC7_BREBN Putative uncharacterized protein yflK 196 1e-48 Q5KZG9.1 Q5KZG9_GEOKA Hypothetical conserved protein 195 2e-48 C3IZS8.1 C3IZS8_9BACI MOSC domain containing protein 195 2e-48 >B3K7N4.1 B3K7N4_9BACI MOSC domain containing protein Length = 219 Score = 257 bits (656), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 122/211 (57%), Positives = 148/211 (70%) Query: 1 MEVISLNVGKPKTVDYRGKPLETGIYKMPAPGKVQLHLEGFDGDGQADLVNHGGADKAVC 60 M ++SLNVGKP TVDY+GK L TGIYK P G + L F+GDGQADL+NHGG DKAVC Sbjct: 3 MGIVSLNVGKPVTVDYQGKDLSTGIYKQPVEGSLFLSSLNFEGDGQADLINHGGLDKAVC 62 Query: 61 VYPIEHYPFWEQWLGKKLEYSAFGENITANGLLETEVCIGDVYEVGTTLLQVSQPRFPCF 120 YP EHYP+WE+ LGK + Y+AFGEN+T GLLE EV IGDVY VG +LQVSQPR+PCF Sbjct: 63 AYPSEHYPYWERSLGKPMPYAAFGENLTLRGLLEDEVRIGDVYRVGEAVLQVSQPRYPCF 122 Query: 121 KLAQKHGPADMPAQVLKTGYSGFYFRVLREGQIAAGDPIIKQESGKGSFPIKQVLYLMEH 180 KL+QKHG DMPA+VL TGYSGFYFRVL EG I+A P+ + +S + +VL +M+ Sbjct: 123 KLSQKHGVKDMPARVLNTGYSGFYFRVLEEGSISADSPVTRLDSQASGITVLEVLRMMKD 182 Query: 181 GRKDQTGXXXXXXXXXXXXDTREKFQGWLDT 211 GRKD+ G + +F WL T Sbjct: 183 GRKDEQGLERMLEIDELASSLKSQFGTWLAT 213 >O34542.1 YFLK_BACSU Uncharacterized protein yflK Length = 221 Score = 206 bits (525), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 98/185 (52%), Positives = 129/185 (69%) Query: 3 VISLNVGKPKTVDYRGKPLETGIYKMPAPGKVQLHLEGFDGDGQADLVNHGGADKAVCVY 62 V+SLN+GKP+T++Y GK +ETGI K PA V L+ E F+GDGQADLVNHGG DKAVCVY Sbjct: 8 VLSLNLGKPQTLEYDGKKIETGIMKRPAKSAVMLYRENFEGDGQADLVNHGGPDKAVCVY 67 Query: 63 PIEHYPFWEQWLGKKLEYSAFGENITANGLLETEVCIGDVYEVGTTLLQVSQPRFPCFKL 122 P EHYPFWE++L + L +AFGEN+T GL E VCIGDV+ + ++QVSQPR PC KL Sbjct: 68 PAEHYPFWEEFLSRPLSNAAFGENLTVAGLTEENVCIGDVFRLDEAVVQVSQPRQPCVKL 127 Query: 123 AQKHGPADMPAQVLKTGYSGFYFRVLREGQIAAGDPIIKQESGKGSFPIKQVLYLMEHGR 182 A+K G +M +V +TGY+GFYFRVL EG+++ G + G+ ++ + H Sbjct: 128 AKKFGVKEMVLKVQQTGYTGFYFRVLEEGRVSPGANLELLSRGEKGISVQFANRINYHDA 187 Query: 183 KDQTG 187 K+ T Sbjct: 188 KNLTA 192 >C0ZDC7.1 C0ZDC7_BREBN Putative uncharacterized protein yflK Length = 227 Score = 196 bits (497), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 90/185 (48%), Positives = 128/185 (69%), Gaps = 1/185 (0%) Query: 1 MEVISLNVGKPKTVDYRGKPLETGIYKMPAPGKVQLHLEGFDGDGQADLVNHGGADKAVC 60 +E++S+NVGKPK V Y+ K + TGIYK P + L +GDGQADLV+HGG +KAVC Sbjct: 3 IEILSINVGKPKQVQYQNKEVSTGIYKTPTTKALYLSFLNLEGDGQADLVHHGGKEKAVC 62 Query: 61 VYPIEHYPFWEQWLGKKLEYSAFGENITANGLLETEVCIGDVYEVGTTLLQVSQPRFPCF 120 VYP EHYPFWE L ++L AFGEN+T GL+ET+VCIGD +++G ++Q+SQPR PC+ Sbjct: 63 VYPYEHYPFWEAQLQRELPIGAFGENLTIRGLVETDVCIGDTFQLGEAVVQISQPRQPCY 122 Query: 121 KLAQKHGPADMPAQVLKTGYSGFYFRVLREGQIAAGDPIIKQESGKGSFPIKQVLYLMEH 180 KL+ ++G +M +V +TGY+G+YFRVL+EG ++ D +I+ + + +M H Sbjct: 123 KLSVRYGVPEMLVKVQETGYTGYYFRVLKEGLVSHTDGLIRLSCHPKAITVSYANRIM-H 181 Query: 181 GRKDQ 185 KD Sbjct: 182 QEKDN 186 >Q5KZG9.1 Q5KZG9_GEOKA Hypothetical conserved protein Length = 215 Score = 195 bits (496), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 98/214 (45%), Positives = 128/214 (59%) Query: 1 MEVISLNVGKPKTVDYRGKPLETGIYKMPAPGKVQLHLEGFDGDGQADLVNHGGADKAVC 60 M+++S+NVGKPKT+ G+ + TGI K P V + + GDGQADLV+HGG DKA+C Sbjct: 1 MQILSINVGKPKTLHIGGQRITTGIDKTPVAHPVAVGKQNLAGDGQADLVHHGGEDKAIC 60 Query: 61 VYPIEHYPFWEQWLGKKLEYSAFGENITANGLLETEVCIGDVYEVGTTLLQVSQPRFPCF 120 YP EH+ +WE+ G+ AFGEN T GL E +VC+GD+Y GT L+QVSQPR PC Sbjct: 61 AYPSEHFVYWEERYGRPFTAGAFGENWTLLGLTEDDVCLGDIYAAGTALVQVSQPRQPCS 120 Query: 121 KLAQKHGPADMPAQVLKTGYSGFYFRVLREGQIAAGDPIIKQESGKGSFPIKQVLYLMEH 180 KLA KH D+P V +TG SGFYFRVL+EG +A G P++ E G G+ I + ++ H Sbjct: 121 KLAFKHQLPDLPKAVCQTGKSGFYFRVLKEGVVAPGAPLVLVERGAGALSIAYINHIYYH 180 Query: 181 GRKDQTGXXXXXXXXXXXXDTREKFQGWLDTAGR 214 R + RE FQ L R Sbjct: 181 ERDNAAAIKQIASHPALSASWREAFQKRLSERTR 214 >C3IZS8.1 C3IZS8_9BACI MOSC domain containing protein Length = 215 Score = 195 bits (496), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 98/214 (45%), Positives = 128/214 (59%) Query: 1 MEVISLNVGKPKTVDYRGKPLETGIYKMPAPGKVQLHLEGFDGDGQADLVNHGGADKAVC 60 M+++S+NVGKPKT+ G+ + TGI K P V + + GDGQADLV+HGG DKA+C Sbjct: 1 MQILSINVGKPKTLHIGGQRITTGIDKTPVAHPVAVGKQNLAGDGQADLVHHGGEDKAIC 60 Query: 61 VYPIEHYPFWEQWLGKKLEYSAFGENITANGLLETEVCIGDVYEVGTTLLQVSQPRFPCF 120 YP EH+ +WE+ G+ AFGEN T GL E +VC+GD+Y GT L+QVSQPR PC Sbjct: 61 AYPSEHFVYWEERYGRPFTAGAFGENWTLLGLTEDDVCLGDIYAAGTALVQVSQPRQPCS 120 Query: 121 KLAQKHGPADMPAQVLKTGYSGFYFRVLREGQIAAGDPIIKQESGKGSFPIKQVLYLMEH 180 KLA KH D+P V +TG SGFYFRVL+EG +A G P++ E G G+ I + ++ H Sbjct: 121 KLAFKHQLPDLPKAVCQTGKSGFYFRVLKEGVVAPGAPLVLVERGAGALSIAYINHIYYH 180 Query: 181 GRKDQTGXXXXXXXXXXXXDTREKFQGWLDTAGR 214 R + RE FQ L R Sbjct: 181 ERDNAAAIKQIASHPALSASWREAFQKRLTARPR 214 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.140 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,339,503,671 Number of extensions: 56756531 Number of successful extensions: 86710 Number of sequences better than 1.0e-05: 654 Number of HSP's gapped: 86198 Number of HSP's successfully gapped: 654 Length of query: 214 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 84 Effective length of database: 1,835,964,253 Effective search space: 154220997252 Effective search space used: 154220997252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)