BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0063.0003.p (252 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2BCX2.1 B2BCX2_PAEPO Pyridoxal-5-P synthase 444 e-123 B3KK87.1 B3KK87_9BACI Pyridoxine biosynthesis protein 436 e-121 B1DKC3.1 B1DKC3_9BACL Pyridoxine biosynthesis protein 430 e-119 Q8L1A8.1 PDXS_BACCI Pyridoxal biosynthesis lyase pdxS EC=4.-.-.- 414 e-114 Q5WKW2.1 PDXS_BACSK Pyridoxal biosynthesis lyase pdxS EC=4.-.-.- 409 e-112 >B2BCX2.1 B2BCX2_PAEPO Pyridoxal-5-P synthase Length = 293 Score = 444 bits (1141), Expect = e-123, Method: Compositional matrix adjust. Identities = 218/251 (86%), Positives = 229/251 (91%) Query: 1 MALERVPSDIRAAGGVARMADPTIVEEVIKVVSIPVMAKARIGHYVEAKVLESLGVDYLD 60 MALERVPSDIRAAGGVARMADPTIVEEV+KVVSIPVMAKARIGH+VEAKVLESLGVDYLD Sbjct: 42 MALERVPSDIRAAGGVARMADPTIVEEVMKVVSIPVMAKARIGHFVEAKVLESLGVDYLD 101 Query: 61 ESEVLTPADEVFHINKREFTVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGNIVEAVR 120 ESEVLTPADEVFHI+KREFTVPFVCGAKDLGEALRRI EGASMIRTKGEPGTGNIVEAVR Sbjct: 102 ESEVLTPADEVFHIDKREFTVPFVCGAKDLGEALRRIGEGASMIRTKGEPGTGNIVEAVR 161 Query: 121 HMRYINSQIRKVTNLSLDELYNEAKTLGVPYDLLLEVHELGKLPVVNFXXXXXXXXXXXX 180 HMR+IN QIRKV NLS DELY EAK LGV Y+LLLEVHELGKLPVVNF Sbjct: 162 HMRFINGQIRKVQNLSKDELYAEAKNLGVAYELLLEVHELGKLPVVNFAAGGVATPADAA 221 Query: 181 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHFTDYKLIAEVSKNLGTPMKGIDIAS 240 LMMHLGADGVFVGSGIFKSD+PEKFARAIVEATTH+TDYKLIAEVSKNLGTPMKGI+I+ Sbjct: 222 LMMHLGADGVFVGSGIFKSDSPEKFARAIVEATTHYTDYKLIAEVSKNLGTPMKGIEISK 281 Query: 241 LSPAERMSERG 251 L+P ERM +RG Sbjct: 282 LAPHERMQDRG 292 >B3KK87.1 B3KK87_9BACI Pyridoxine biosynthesis protein Length = 293 Score = 436 bits (1121), Expect = e-121, Method: Compositional matrix adjust. Identities = 215/251 (85%), Positives = 224/251 (89%) Query: 1 MALERVPSDIRAAGGVARMADPTIVEEVIKVVSIPVMAKARIGHYVEAKVLESLGVDYLD 60 MALERVPSDIRAAGGVARMADPTIVEEV+KVVSIPVMAKARIGHYVEAKVLESLGVDYLD Sbjct: 42 MALERVPSDIRAAGGVARMADPTIVEEVMKVVSIPVMAKARIGHYVEAKVLESLGVDYLD 101 Query: 61 ESEVLTPADEVFHINKREFTVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGNIVEAVR 120 ESEVLTPADEVFHI+K +FTVPFVCGAKD+GEALRRI EGASMIRTKGEPGTGNIVEAVR Sbjct: 102 ESEVLTPADEVFHIDKWDFTVPFVCGAKDIGEALRRIGEGASMIRTKGEPGTGNIVEAVR 161 Query: 121 HMRYINSQIRKVTNLSLDELYNEAKTLGVPYDLLLEVHELGKLPVVNFXXXXXXXXXXXX 180 HMR INSQIRKV N+S DELY EAK LGV YDLL VHE GKLPVVNF Sbjct: 162 HMRLINSQIRKVRNMSKDELYAEAKNLGVSYDLLKGVHETGKLPVVNFAAGGVATPADAA 221 Query: 181 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHFTDYKLIAEVSKNLGTPMKGIDIAS 240 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTH+ DYKLIAEVSKNLG PMKGI+I+ Sbjct: 222 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHYDDYKLIAEVSKNLGAPMKGIEISK 281 Query: 241 LSPAERMSERG 251 LSPAERM +RG Sbjct: 282 LSPAERMQDRG 292 >B1DKC3.1 B1DKC3_9BACL Pyridoxine biosynthesis protein Length = 293 Score = 430 bits (1106), Expect = e-119, Method: Compositional matrix adjust. Identities = 209/251 (83%), Positives = 224/251 (89%) Query: 1 MALERVPSDIRAAGGVARMADPTIVEEVIKVVSIPVMAKARIGHYVEAKVLESLGVDYLD 60 MALERVPSDIRAAGGVARMADPTIVEEV+KVVSIPVMAKARIGH VEA+VLES+GVDY+D Sbjct: 42 MALERVPSDIRAAGGVARMADPTIVEEVMKVVSIPVMAKARIGHIVEARVLESMGVDYID 101 Query: 61 ESEVLTPADEVFHINKREFTVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGNIVEAVR 120 ESEVLTPADEVFHI+K EFT+PFVCGAKDLGEALRRI EGA+M+RTKGEPGTGNIVEAVR Sbjct: 102 ESEVLTPADEVFHISKNEFTIPFVCGAKDLGEALRRIQEGAAMLRTKGEPGTGNIVEAVR 161 Query: 121 HMRYINSQIRKVTNLSLDELYNEAKTLGVPYDLLLEVHELGKLPVVNFXXXXXXXXXXXX 180 H+R IN QIRKV NLS DELYNEAK LGVPYDLLL+VHE GKLPVVNF Sbjct: 162 HLRLINGQIRKVQNLSKDELYNEAKVLGVPYDLLLQVHESGKLPVVNFAAGGVATPSDAA 221 Query: 181 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHFTDYKLIAEVSKNLGTPMKGIDIAS 240 LMM LGADGVFVGSGIFKSD+PEKFARAIVEATTH+ DYKLIAEVSKNLGTPMKGI+I+ Sbjct: 222 LMMELGADGVFVGSGIFKSDSPEKFARAIVEATTHYKDYKLIAEVSKNLGTPMKGIEISK 281 Query: 241 LSPAERMSERG 251 L AERM +RG Sbjct: 282 LQAAERMQDRG 292 >Q8L1A8.1 PDXS_BACCI Pyridoxal biosynthesis lyase pdxS EC=4.-.-.- Length = 293 Score = 414 bits (1065), Expect = e-114, Method: Compositional matrix adjust. Identities = 199/251 (79%), Positives = 221/251 (88%) Query: 1 MALERVPSDIRAAGGVARMADPTIVEEVIKVVSIPVMAKARIGHYVEAKVLESLGVDYLD 60 MALERVPSDIRAAGGVARMADPT++EEV+KVVSIPVMAKARIGH VEA+VLE+LG DY+D Sbjct: 42 MALERVPSDIRAAGGVARMADPTVLEEVMKVVSIPVMAKARIGHIVEARVLEALGADYID 101 Query: 61 ESEVLTPADEVFHINKREFTVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGNIVEAVR 120 ESEVLTPADEVFHI+K++FTVPFVCGAKD+GEALRRINEGASM+RTKGEPGTGNIVEAVR Sbjct: 102 ESEVLTPADEVFHIDKKDFTVPFVCGAKDIGEALRRINEGASMLRTKGEPGTGNIVEAVR 161 Query: 121 HMRYINSQIRKVTNLSLDELYNEAKTLGVPYDLLLEVHELGKLPVVNFXXXXXXXXXXXX 180 HMR I SQIRK+ +L+ DELY EAK LG PYDL+L +HE GKLPVVNF Sbjct: 162 HMRLIQSQIRKIQSLAKDELYAEAKNLGAPYDLVLYIHENGKLPVVNFAAGGVATPSDAA 221 Query: 181 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHFTDYKLIAEVSKNLGTPMKGIDIAS 240 LMM LGADGVFVGSGIFKSD+PEKFARAIVEATTH+ DYKLIAEVSKNLG PMKGI+I+ Sbjct: 222 LMMELGADGVFVGSGIFKSDSPEKFARAIVEATTHYKDYKLIAEVSKNLGAPMKGIEISK 281 Query: 241 LSPAERMSERG 251 L +ERM +RG Sbjct: 282 LHASERMQDRG 292 >Q5WKW2.1 PDXS_BACSK Pyridoxal biosynthesis lyase pdxS EC=4.-.-.- Length = 295 Score = 409 bits (1050), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/251 (76%), Positives = 219/251 (87%) Query: 1 MALERVPSDIRAAGGVARMADPTIVEEVIKVVSIPVMAKARIGHYVEAKVLESLGVDYLD 60 MALERVPSDIRAAGGVARMADPT+ EEV+ VSIPVMAKARIGH VEAKVLE++GVDY+D Sbjct: 44 MALERVPSDIRAAGGVARMADPTVTEEVLNAVSIPVMAKARIGHIVEAKVLEAMGVDYID 103 Query: 61 ESEVLTPADEVFHINKREFTVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGNIVEAVR 120 ESEVLTPADEV+H+NKR+FTVPFVCGA+DLGEA RRI EGASMIRTKGEPGTGNIVEAVR Sbjct: 104 ESEVLTPADEVYHLNKRDFTVPFVCGARDLGEAARRIGEGASMIRTKGEPGTGNIVEAVR 163 Query: 121 HMRYINSQIRKVTNLSLDELYNEAKTLGVPYDLLLEVHELGKLPVVNFXXXXXXXXXXXX 180 HMR + +QI++V ++S DEL EAK LG PY+LLL + E G+LPVVNF Sbjct: 164 HMRKMQAQIKQVAHMSTDELMTEAKQLGAPYELLLHIKETGQLPVVNFAAGGIATPADAA 223 Query: 181 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHFTDYKLIAEVSKNLGTPMKGIDIAS 240 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTH+TDY+ IAE+SKNLG+ MKGID+A+ Sbjct: 224 LMMHLGADGVFVGSGIFKSDNPEKFARAIVEATTHYTDYERIAELSKNLGSAMKGIDVAT 283 Query: 241 LSPAERMSERG 251 L+PAERM +RG Sbjct: 284 LAPAERMQDRG 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,347,410,886 Number of extensions: 49970604 Number of successful extensions: 134942 Number of sequences better than 1.0e-05: 658 Number of HSP's gapped: 134566 Number of HSP's successfully gapped: 664 Length of query: 252 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 120 Effective length of database: 1,817,122,223 Effective search space: 218054666760 Effective search space used: 218054666760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)