BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0061.0007.p (474 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7D2.1 B3K7D2_9BACI VanW family protein 402 e-110 B1DE82.1 B1DE82_9BACL VanW family protein 323 2e-86 A8MEY0.1 A8MEY0_ALKOO VanW family protein 187 2e-45 A6TU80.1 A6TU80_ALKMQ VanW family protein 182 6e-44 B1I375.1 B1I375_DESAP VanW family protein 181 1e-43 >B3K7D2.1 B3K7D2_9BACI VanW family protein Length = 478 Score = 402 bits (1033), Expect = e-110, Method: Compositional matrix adjust. Identities = 210/451 (46%), Positives = 289/451 (64%), Gaps = 7/451 (1%) Query: 27 LYGSQQTLPPKTLVAGWDVGGMEIADVRAGLATRLQALGAVPLVLKAEP--NTRLTVTLR 84 +Y +Q+ LP +++GW VGG +V + L RL A+ ++ + L++E +L+ Sbjct: 27 MYANQKELPKGVVLSGWQVGGQPADEVLSELEQRLHAMTSIKVTLESEALKGHAENFSLQ 86 Query: 85 QAGMTYEAEKFXXXXXXXXXXXXXXRVRARWSFPRSWELGVHLDLAQLQKSLSPDWEKET 144 +AG+ + A F +V R F W + + L+ L+ DW+KE Sbjct: 87 EAGVRFSAADFREAIAKLSEGSLWEQVLYRRQFQLEWHISTDWNRDVLKGRLNEDWQKER 146 Query: 145 FGVPVDAVRRITADDRVVYTPEKTTYEVDWRALELILQAAVPLSLHNADSLKGKQIMLEV 204 FG PV+AVR I+ DD V YTPEKT Y +DW A AA+P H + + I +++ Sbjct: 147 FGEPVNAVRSISEDDVVRYTPEKTAYRIDWPAFYEGFSAALPNESHFTEPYPEEAIAVQL 206 Query: 205 PLSIRQPEVTLKILKEQGIERKISQFSTSLGASGPGRTFNIQAAAEAVNGTLLPPGAVFD 264 PL ++P VT+K L+++GIERKI +FST LG+SGPGR N+ AAA+AV+G +L PG F+ Sbjct: 207 PLIRQKPPVTVKSLQDEGIERKIVEFSTGLGSSGPGRVHNVTAAAKAVDGMILKPGDEFN 266 Query: 265 YGKAIQKAQADTGFREAPVIVNGRLQPGVGGGICQVSSTLYNAALRSGLEIVERRNHSLP 324 Y I A+ + GF EAPVIVNG+L PG+GGGICQVSST+Y+AAL GLEI ERRNHSLP Sbjct: 267 YAHIIDVAKKEYGFEEAPVIVNGKLVPGIGGGICQVSSTVYHAALLIGLEITERRNHSLP 326 Query: 325 VNYLPKGQDATFSEGNINFRFRNNTGKSLIIHAAVQGRTLTVKLFGTFPKNVTFLVQSRT 384 V+YLPKGQDATF+EG INFRFRNNTGK L+I + V LT+K+FGTFP+N + + S T Sbjct: 327 VSYLPKGQDATFAEGAINFRFRNNTGKHLLILSEVSHGNLTLKMFGTFPENTEYELVSTT 386 Query: 385 VELLAPADKYVSDPSLPRGGTRVIQNGRTGYIVETYITRLVDGKAVEKKLLSRDTYYAQK 444 +E L +K + + SLP G +V+QNG+ GY+VETY T+ V+GK VE +S+DTY Q Sbjct: 387 IETLPSPEKVIHNNSLPPGLKQVVQNGKPGYVVETYRTKKVNGKVVENVKISKDTYRPQN 446 Query: 445 RIIAINRGGMGKSIQPDSRRQP---LVEDGV 472 I+AIN + PD+ P +VEDG+ Sbjct: 447 SIVAINP--TNEVPLPDANETPKEEVVEDGI 475 >B1DE82.1 B1DE82_9BACL VanW family protein Length = 476 Score = 323 bits (829), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 182/431 (42%), Positives = 258/431 (59%), Gaps = 13/431 (3%) Query: 28 YGSQQTLPPKTLVAGWDVGGMEIADVRAGLATRLQALGAVPLVLK--AEPNTRLTVTLRQ 85 Y Q T+P K G D+GG+ I D L Q L + + ++ AE + T + Sbjct: 29 YSGQDTVPEKVTAGGVDIGGLSIDDALKKLDQYEQMLESRTVTVEGNAEAKAEGSQTWKA 88 Query: 86 AGMTYEAE--KFXXXXXXXXXXXXXXRVRARWSFPRSWELGVHLDLAQLQKSLSPDWEKE 143 A + Y AE R + R+ F S+EL D A K + +++ Sbjct: 89 AEIGYNAEFVGVKEELKKLRTGNVWTRSKFRYHFTTSFELKQSYDPAVFDKLVLAEFDWI 148 Query: 144 TFGVPVDAVRRITADDRVVYTPEKTTYEVDWRALELILQAAVPLSLHNADSLKGKQ--IM 201 PV+A R+IT +D VVYT Y +D LE + + + + DS G Q + Sbjct: 149 DSNKPVNATRKITDNDEVVYTSHTDAYRIDVAPLEEKVASWITV----PDSQLGGQPEQV 204 Query: 202 LEVPLSIR--QPEVTLKILKEQGIERKISQFSTSLGASGPGRTFNIQAAAEAVNGTLLPP 259 L V L ++ PE+TL+ LK++GI+RKI +F+T+ S GR FN+ + A+A+N +L P Sbjct: 205 LTVQLGVKTVHPEITLEKLKDEGIDRKIMEFTTNFATSAEGRAFNVTSTAKALNDWVLEP 264 Query: 260 GAVFDYGKAIQKAQADTGFREAPVIVNGRLQPGVGGGICQVSSTLYNAALRS-GLEIVER 318 G VF YG + K + + G++EAPVI NG+L PG+GGGICQVSSTLYNA +R+ G++IVER Sbjct: 265 GEVFSYGDVVAKTEKEYGYKEAPVISNGKLVPGIGGGICQVSSTLYNAIIRTAGIDIVER 324 Query: 319 RNHSLPVNYLPKGQDATFSEGNINFRFRNNTGKSLIIHAAVQGRTLTVKLFGTFPKNVTF 378 RNHSLPV YLPKGQDATF+ G+I+FRFRN+TGK LII V+ R LTVKLFGT P+N+ + Sbjct: 325 RNHSLPVAYLPKGQDATFASGSIDFRFRNSTGKQLIIRTVVENRKLTVKLFGTMPENIRY 384 Query: 379 LVQSRTVELLAPADKYVSDPSLPRGGTRVIQNGRTGYIVETYITRLVDGKAVEKKLLSRD 438 ++S V+ + PA + V+D SL G + +QNG GY+V+TY T DGK V ++ +S D Sbjct: 385 DIESSIVKTVQPAVQKVTDKSLGAGQSVTVQNGSIGYVVDTYRTMYKDGKQVSREKISHD 444 Query: 439 TYYAQKRIIAI 449 TY AQ ++ + Sbjct: 445 TYRAQPIVVHV 455 >A8MEY0.1 A8MEY0_ALKOO VanW family protein Length = 453 Score = 187 bits (475), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 99/255 (38%), Positives = 154/255 (60%), Gaps = 3/255 (1%) Query: 187 LSLHNADSLKGKQIM-LEVPLSIRQPEVTLKILKEQGIERKISQFSTSLGASGPGRTFNI 245 L H ++K Q + ++VP+ P + + LK G I QFST+ GR NI Sbjct: 187 LKEHIVRAIKENQNLPIQVPVQYIDPLIKEEDLKMIG--PLIGQFSTAFNLEQTGRVENI 244 Query: 246 QAAAEAVNGTLLPPGAVFDYGKAIQKAQADTGFREAPVIVNGRLQPGVGGGICQVSSTLY 305 + AA++++GT+L PG F + ++ + G++EA VIVNG PGVGGGICQVS+TLY Sbjct: 245 KLAAQSIDGTVLMPGEEFSFNESTGPRSVEEGYQEALVIVNGEFVPGVGGGICQVSTTLY 304 Query: 306 NAALRSGLEIVERRNHSLPVNYLPKGQDATFSEGNINFRFRNNTGKSLIIHAAVQGRTLT 365 A +RSG+EI RRNH LPV Y+P G DAT + G I+F+F+NN + I + V+ + Sbjct: 305 QAVVRSGIEITARRNHGLPVGYVPMGHDATVAYGYIDFKFKNNKNHPIYIESYVKDNKIY 364 Query: 366 VKLFGTFPKNVTFLVQSRTVELLAPADKYVSDPSLPRGGTRVIQNGRTGYIVETYITRLV 425 VKL+G +++ + S TVE++ P + DP++ G ++ + + GY V TY L Sbjct: 365 VKLYGKEAEDILIDLISETVEVIEPKMEIKKDPNMYVGERKISKASKKGYRVFTYKIYLQ 424 Query: 426 DGKAVEKKLLSRDTY 440 +GK ++++L+++D Y Sbjct: 425 NGKEIKRELITKDYY 439 >A6TU80.1 A6TU80_ALKMQ VanW family protein Length = 485 Score = 182 bits (462), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 111/287 (38%), Positives = 162/287 (56%), Gaps = 11/287 (3%) Query: 162 VYTPEKTTYEVDWRALELILQAAVPLSLHNADSLKGKQIMLEVPLSIRQPEVTLKILKEQ 221 V T E+ +VD L ++ A+ A S G +L VP+ VT + LK Sbjct: 172 VITEEEVGVQVDQVLLRTRIREAL------ASSYSG---ILPVPVKHVLASVTREDLK-- 220 Query: 222 GIERKISQFSTSLGASGPGRTFNIQAAAEAVNGTLLPPGAVFDYGKAIQKAQADTGFREA 281 IE I +F+T+ A GRT NI+ + +++GTL+ PG +F + + + G++EA Sbjct: 221 VIEVTIGEFATNFNAQQEGRTKNIEIGSSSIDGTLIMPGEIFSFNEKTGPRSVEEGYQEA 280 Query: 282 PVIVNGRLQPGVGGGICQVSSTLYNAALRSGLEIVERRNHSLPVNYLPKGQDATFSEGNI 341 PVI+ GRL PGVGGGICQVS+TLYNA +R LEIV R+NHSLPV+Y+P G DAT + G + Sbjct: 281 PVILAGRLVPGVGGGICQVSTTLYNAVIRGDLEIVNRQNHSLPVSYVPLGHDATVAYGYL 340 Query: 342 NFRFRNNTGKSLIIHAAVQGRTLTVKLFGTFPKNVTFLVQSRTVELLAPADKYVSDPSLP 401 +F F+N+T + I + V G L V++FG + S VE ++P + DP+L Sbjct: 341 DFEFKNSTEHPIYIESTVLGNRLEVRIFGKNVPPYQVRLASEIVERISPTREVKQDPNLL 400 Query: 402 RGGTRVIQNGRTGYIVETYITRLVDGKAVEKKLLSRDTYYAQKRIIA 448 G +V Q G+ GY V+T + + ++ +S D Y K IIA Sbjct: 401 VGERKVQQEGKQGYRVKTDKVYYEGNQEIRREQISTDYYPPVKTIIA 447 >B1I375.1 B1I375_DESAP VanW family protein Length = 491 Score = 181 bits (460), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 131/434 (30%), Positives = 200/434 (46%), Gaps = 34/434 (7%) Query: 29 GSQQTLPPKTLVAGWDVGGMEIADVRAGLATRLQALGAVPLVLKAEPNTRLTVTLRQAGM 88 G QT+ P V G D+GG++ A + + Q+L PL L + V L G+ Sbjct: 33 GRSQTVFPGVSVYGLDLGGLDQAGCLEAVGSLEQSLRCTPLTL-VYGSRSWRVELGDLGL 91 Query: 89 TYEAEKFXXXXXXXXXXXXXXRVRARWSFPRSWELGVHLDLAQLQKSLSPDWEKETFGV- 147 + +K F W + + L D+E+ F Sbjct: 92 VVDGDKITSEALKAGRSGF---------FVSQWLERGEISRNGFKLDLCADFEEPRFRTV 142 Query: 148 -----------PVDAVRRITADDRVVYTPEKTTYEVDW-RALELILQAAVPLSLHNADSL 195 PVDA I DD V P + Y+VD R + ILQ Sbjct: 143 IDEITTEITRAPVDARYHIGNDDTVHIIPHQDGYQVDHGRLKQRILQQLE---------- 192 Query: 196 KGKQIMLEVPLSIRQPEVTLKILKEQGIERKISQFSTSLGASGPGRTFNIQAAAEAVNGT 255 G+ + +P+ PE T ++ + +++F T+ + R++NI AA A+N Sbjct: 193 TGRLAPVSIPVVHVVPEYTTSDVEAMNLTGLLAEFGTNYDPAVVNRSYNISVAAGALNNL 252 Query: 256 LLPPGAVFDYGKAIQKAQADTGFREAPVIVNGRLQPGVGGGICQVSSTLYNAALRSGLEI 315 L+PPG + + + + ++ G+R APVI+ + Q G+GGG+CQVSSTLYNA + + LE+ Sbjct: 253 LIPPGEIVSFNRIVGPRSSEAGYRTAPVIIENQFQEGIGGGVCQVSSTLYNAVVLANLEV 312 Query: 316 VERRNHSLPVNYLPKGQDATFSEGNINFRFRNNTGKSLIIHAAVQGRTLTVKLFGTFPKN 375 +ER+NHSLPV Y+P G+DAT G I+ RFRNNTG + + + ++VK+FG + Sbjct: 313 LERKNHSLPVTYVPIGRDATVVFGAIDLRFRNNTGGYIYLKTSAANGRVSVKIFGDHRYH 372 Query: 376 VTFLVQSRTVELLAPADKYVSDPSLPRGGTRVIQNGRTGYIVETYITRLVDGKAVEKKLL 435 ++S E L P Y D +L +G V Q G GY + +G A EK+ L Sbjct: 373 PRVEIKSWITETLEPRTVYEDDENLEKGKQVVKQAGLRGYRAQAERWVWQNG-AAEKEPL 431 Query: 436 SRDTYYAQKRIIAI 449 Y+ IIA+ Sbjct: 432 PASFYHPVPEIIAV 445 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,629,536,707 Number of extensions: 106875579 Number of successful extensions: 255087 Number of sequences better than 1.0e-05: 521 Number of HSP's gapped: 254850 Number of HSP's successfully gapped: 521 Length of query: 474 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 334 Effective length of database: 1,741,754,103 Effective search space: 581745870402 Effective search space used: 581745870402 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)