BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0013.p (312 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K907.1 B3K907_9BACI Cysteine synthase EC=2.5.1.47 521 e-146 B1DID7.1 B1DID7_9BACL Cysteine synthase EC=2.5.1.47 482 e-134 C0ZIC9.1 C0ZIC9_BREBN Cysteine synthase EC=2.5.1.47 434 e-120 Q9F983.1 Q9F983_BACST Cysteine synthase EC=2.5.1.47 410 e-113 C5D393.1 C5D393_9BACI Cysteine synthase A 404 e-111 >B3K907.1 B3K907_9BACI Cysteine synthase EC=2.5.1.47 Length = 311 Score = 521 bits (1342), Expect = e-146, Method: Compositional matrix adjust. Identities = 261/311 (83%), Positives = 283/311 (90%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 MAKVVN+VT+LIG TPLVRLNR+VPE SAEIYLKLEYQNPG+SVKDRIAISIVEEAEK+G Sbjct: 1 MAKVVNNVTELIGETPLVRLNRIVPEDSAEIYLKLEYQNPGASVKDRIAISIVEEAEKDG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LKPG TI+EATSGNTGIGLA+VAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS Sbjct: 61 RLKPGDTIIEATSGNTGIGLAMVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 EGMNGAVKKAE+IL EN +F+AEQFKN+ANVKIHRETTGPEIVEAI+SIGG LDAF Sbjct: 121 EGMNGAVKKAEEILKENAGFFMAEQFKNQANVKIHRETTGPEIVEAIQSIGGSLDAFVAG 180 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 EVLK +YP++KI AVEPAASPILAGGKPGPHKIQGIGANFIP+ILNQ V Sbjct: 181 IGTGGTITGAGEVLKEQYPDIKIIAVEPAASPILAGGKPGPHKIQGIGANFIPEILNQEV 240 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YDEI+HVEND+AFET+RRVAKEEGVL GISSGAAV+AALKVAKELG GK+VV +IPSNGE Sbjct: 241 YDEIVHVENDDAFETSRRVAKEEGVLGGISSGAAVYAALKVAKELGKGKRVVAVIPSNGE 300 Query: 301 RYLSTPLYNFE 311 RYLSTPLYNFE Sbjct: 301 RYLSTPLYNFE 311 >B1DID7.1 B1DID7_9BACL Cysteine synthase EC=2.5.1.47 Length = 312 Score = 482 bits (1241), Expect = e-134, Method: Compositional matrix adjust. Identities = 236/311 (75%), Positives = 272/311 (87%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 MA++VNSVT+LIG TPLVRLNR+VPE SAEIY+KLEYQNPG+SVKDRIAIS++E AE+EG Sbjct: 1 MARIVNSVTELIGDTPLVRLNRLVPEDSAEIYVKLEYQNPGASVKDRIAISMIEVAEQEG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 +LKPG TIVE TSGNTGIGLA+VAAAKGYKA++VMPETMS+ERRNLLRAYGAELVLTPGS Sbjct: 61 VLKPGDTIVEPTSGNTGIGLAMVAAAKGYKAILVMPETMSIERRNLLRAYGAELVLTPGS 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 EGMNGAV+KAE++ ENP YF+ +QFKN+ANVK+HRETTGPEIVEAI S+ G LDAF Sbjct: 121 EGMNGAVRKAEELAKENPSYFIPQQFKNQANVKVHRETTGPEIVEAINSLDGKLDAFVAG 180 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 EVLK + +KIYAVEPAASPIL+GG PGPHKIQGIGANF+PDILN+ + Sbjct: 181 IGTGGTISGTGEVLKKNFEGIKIYAVEPAASPILSGGNPGPHKIQGIGANFVPDILNREI 240 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YD +I VEN+EAFE ARR AKEEG+L GISSGAA++AALKVAKELG GK+V+ IIPSNGE Sbjct: 241 YDGVITVENEEAFEYARRAAKEEGLLVGISSGAAIYAALKVAKELGKGKRVIAIIPSNGE 300 Query: 301 RYLSTPLYNFE 311 RYLSTPL+NFE Sbjct: 301 RYLSTPLFNFE 311 >C0ZIC9.1 C0ZIC9_BREBN Cysteine synthase EC=2.5.1.47 Length = 310 Score = 434 bits (1115), Expect = e-120, Method: Compositional matrix adjust. Identities = 212/309 (68%), Positives = 256/309 (82%), Gaps = 1/309 (0%) Query: 3 KVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEGLL 62 +V NS+TDLIG TPLV+LNRVV E A+IYLKLE+ NPGSSVKDRIA+S++E AE +G L Sbjct: 2 RVANSITDLIGFTPLVKLNRVVTEDIADIYLKLEFFNPGSSVKDRIALSMIEAAEADGSL 61 Query: 63 KPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGSEG 122 KPG TI+E TSGNTGIGLA+VAAAKGY+A++VMPETMS+ERRNLLRAYGAELVLTPGSEG Sbjct: 62 KPGDTIIEPTSGNTGIGLAMVAAAKGYRAILVMPETMSMERRNLLRAYGAELVLTPGSEG 121 Query: 123 MNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXXXX 182 M GA++KAE++ E+ YF+ +QFKN AN IHRETT E+++ + IGG +DAF Sbjct: 122 MGGAIRKAEELAKEDSSYFIPQQFKNLANPAIHRETTARELLDQAKEIGG-VDAFISGIG 180 Query: 183 XXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNVYD 242 +VL+ YPNV+I AVEPAASP+L+GGKPGPHKIQGIGA F+PDIL+ +YD Sbjct: 181 TGGTITGVGQVLREHYPNVQIVAVEPAASPVLSGGKPGPHKIQGIGAGFVPDILDTQIYD 240 Query: 243 EIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGERY 302 EII VEN++AFETARRVA++EG+L GISSGAA+ AAL+VA +LG GKKV+ IIPSNGERY Sbjct: 241 EIIKVENEDAFETARRVARQEGILGGISSGAAIHAALQVAAKLGKGKKVIAIIPSNGERY 300 Query: 303 LSTPLYNFE 311 LSTPLY FE Sbjct: 301 LSTPLYQFE 309 >Q9F983.1 Q9F983_BACST Cysteine synthase EC=2.5.1.47 Length = 308 Score = 410 bits (1055), Expect = e-113, Method: Compositional matrix adjust. Identities = 213/311 (68%), Positives = 258/311 (82%), Gaps = 4/311 (1%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 MA+ VNS+T+LIG TP V+LNR+V E SA++YLKLE+ NPGSSVKDRIA++++E AEKEG Sbjct: 1 MARTVNSITELIGDTPAVKLNRIVDEDSADVYLKLEFMNPGSSVKDRIALAMIEAAEKEG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LKPG TI+E TSGNTGIGLA+VAAAKGYKA++VMP+TMSLERRNLLRAYGAELVLTPGS Sbjct: 61 KLKPGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPDTMSLERRNLLRAYGAELVLTPGS 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 +GM GA++KAE+++ E+ YF+ +QFKN+AN +IHR TTG EIV E +G LDAF Sbjct: 121 QGMRGAIEKAEELVREH-GYFMPQQFKNEANPEIHRLTTGKEIV---EQMGDQLDAFIAG 176 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 +VL+ KYPN+KIYAVEPA SP+L+GGKPGPHKIQGIGA F+PD+L+ N+ Sbjct: 177 VGTGGTITGAGQVLREKYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDVLDTNI 236 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YDE+I V +EAF ARR A+EEG+L GISSGAA+ AALKVAK+LG GKKV+ IIPSNGE Sbjct: 237 YDEVITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKQLGKGKKVLAIIPSNGE 296 Query: 301 RYLSTPLYNFE 311 RYLSTPLY FE Sbjct: 297 RYLSTPLYQFE 307 >C5D393.1 C5D393_9BACI Cysteine synthase A Length = 308 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 214/311 (68%), Positives = 254/311 (81%), Gaps = 4/311 (1%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 MA+ VNS+T+LIG TP V+LNR+V E SA++YLKLE+ NPGSSVKDRIA++++E AEK G Sbjct: 1 MARTVNSITELIGDTPAVKLNRIVDEDSADVYLKLEFMNPGSSVKDRIALAMIEAAEKAG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LKPG TIVE TSGNTGIGLA+VAAAKGYKAV+VMP+TMSLERRNLLRAYGAELVLTPGS Sbjct: 61 KLKPGDTIVEPTSGNTGIGLAMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGS 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 EGM GA+ KAE+++ E+ YF+ +QFKN+AN +IHR TTG EIV E +G LDAF Sbjct: 121 EGMRGAIAKAEELVREH-GYFMPQQFKNEANPEIHRLTTGKEIV---EQMGDQLDAFIAG 176 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 +VL+ YPN+KIYAVEPA SP+L+GGKPGPHKIQGIGA F+PDIL+ ++ Sbjct: 177 VGTGGTITGAGQVLRETYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSI 236 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YD +I V +EAF ARR A+EEG+L GISSGAA+ AALKVAKELG GKKV+ IIPSNGE Sbjct: 237 YDGVITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAIIPSNGE 296 Query: 301 RYLSTPLYNFE 311 RYLSTPLY FE Sbjct: 297 RYLSTPLYQFE 307 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.134 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,805,763,986 Number of extensions: 72493636 Number of successful extensions: 211382 Number of sequences better than 1.0e-05: 4153 Number of HSP's gapped: 203160 Number of HSP's successfully gapped: 4187 Length of query: 312 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 177 Effective length of database: 1,788,859,178 Effective search space: 316628074506 Effective search space used: 316628074506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 131 (55.1 bits)