BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0009.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K902.1 B3K902_9BACI Nicotinate-nucleotide pyrophosphorylase EC... 460 e-128 C0ZHG9.1 C0ZHG9_BREBN Nicotinate-nucleotide pyrophosphorylase EC... 318 4e-85 B0TBP9.1 B0TBP9_HELMI Nicotinate-nucleotide pyrophosphorylase 311 5e-83 Q8GE44.1 Q8GE44_HELMO Nicotinate-nucleotide pyrophosphorylase EC... 300 1e-79 A5D5U1.1 A5D5U1_PELTS Nicotinate-nucleotide pyrophosphorylase 296 1e-78 >B3K902.1 B3K902_9BACI Nicotinate-nucleotide pyrophosphorylase EC=2.4.2.19 Length = 291 Score = 460 bits (1183), Expect = e-128, Method: Compositional matrix adjust. Identities = 225/285 (78%), Positives = 250/285 (87%) Query: 1 MFNGYNEELVQTIKGWLHEDVGSGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVF 60 MFNGYN+ L+Q+I+ WL ED+GSGDITT TTI GHESKG+IHAKE GI+ G+PVAELVF Sbjct: 2 MFNGYNDGLLQSIRTWLQEDIGSGDITTLTTIEPGHESKGVIHAKEAGIVAGMPVAELVF 61 Query: 61 EVVDPSLVFTALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFV 120 E VDP+L+FTALV++G V KGT++AEVEGSTH ILTGERLALNL+QRLSG+A++T SFV Sbjct: 62 ETVDPTLMFTALVRDGDVVEKGTVLAEVEGSTHRILTGERLALNLLQRLSGIATKTRSFV 121 Query: 121 QKLEGLPSRLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQA 180 LEGLP+RLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGI QA Sbjct: 122 DVLEGLPTRLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIAQA 181 Query: 181 VGRARANIPHTMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVK 240 V RARA+IPHTMTI ALAAGADIIMLDNMS ++MTEAVRRIK K+PHV+ Sbjct: 182 VSRARAHIPHTMTIEVETESLAQVEEALAAGADIIMLDNMSHDMMTEAVRRIKAKSPHVR 241 Query: 241 TEASGNVSLETVRRIAETGVDVISVGRLTYSFSSLDISLDLNAKK 285 EASGNV+LETVR IAETGVDVISVGRLTYSF SLDISLDLNAKK Sbjct: 242 VEASGNVTLETVRGIAETGVDVISVGRLTYSFHSLDISLDLNAKK 286 >C0ZHG9.1 C0ZHG9_BREBN Nicotinate-nucleotide pyrophosphorylase EC=2.4.2.19 Length = 282 Score = 318 bits (815), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 173/285 (60%), Positives = 205/285 (71%), Gaps = 5/285 (1%) Query: 1 MFNGYNEELVQTIKGWLHEDVGSGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVF 60 M+N EL + I+ WL EDVG GD+TT +TIP + GI++AKE GI+ G+P+AE VF Sbjct: 2 MWN--KRELQRKIEEWLQEDVGFGDVTTMSTIPESEQGVGILYAKEAGIVAGLPIAEQVF 59 Query: 61 EVVDPSLVFTALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFV 120 VD +LVF A V+EG V G IAEV GS +IL+GERLALNLMQRLSG+A++T + Sbjct: 60 ATVDSTLVFEAKVEEGARVEVGQQIAEVSGSVRSILSGERLALNLMQRLSGIATKTSEYA 119 Query: 121 QKLEGLPSRLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQA 180 + G +R+VDTRKTTPG R LEKYAVRVGGG NHRF LYDAVMIKDNHIKGAGGI QA Sbjct: 120 TAVAGTKARVVDTRKTTPGLRALEKYAVRVGGGYNHRFALYDAVMIKDNHIKGAGGIAQA 179 Query: 181 VGRARANIPHTMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVK 240 V ARA IPHTMT+ ALAAGAD IMLDNMS + M EAV+ I +A Sbjct: 180 VAAARAVIPHTMTVEVEAESFEQVQEALAAGADTIMLDNMSLDQMVEAVQFINGRAV--- 236 Query: 241 TEASGNVSLETVRRIAETGVDVISVGRLTYSFSSLDISLDLNAKK 285 EASG VSLET+ IA+TGVD+ISVG LT+S + DISLDLN +K Sbjct: 237 VEASGGVSLETIGDIAKTGVDIISVGALTHSVKAFDISLDLNTRK 281 >B0TBP9.1 B0TBP9_HELMI Nicotinate-nucleotide pyrophosphorylase Length = 288 Score = 311 bits (796), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 157/281 (55%), Positives = 204/281 (72%), Gaps = 3/281 (1%) Query: 5 YNEELVQTIKGWLHEDVGSGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVFEVVD 64 + E+ + + L ED+G GD+TT + +PA ++KGIIHAKE G+I G+PVA +VFE VD Sbjct: 4 FEPEVREVVLRALREDIGQGDLTTMSLVPADAQTKGIIHAKEAGVIAGMPVARIVFETVD 63 Query: 65 PSLVFTALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLE 124 P L+F A V++G+ + KGT++AEV GS +IL GERLALN +QRLSG+A++T V+++ Sbjct: 64 PGLIFEAKVKDGERIDKGTVLAEVRGSARSILIGERLALNFLQRLSGIATKTARCVEQVT 123 Query: 125 GLPSRLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRA 184 +R+VDTRKTTPG RMLEKYAVR+GGG NHRFGL+D V+IKDNHI+ AGGI QAV + Sbjct: 124 YYQARIVDTRKTTPGLRMLEKYAVRMGGGKNHRFGLFDGVLIKDNHIRVAGGIHQAVSQV 183 Query: 185 RANIPHTMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEAS 244 R +PHT+ I AL A ADIIMLDNM+PELM EAVR I ++A EAS Sbjct: 184 RQAVPHTVKIEVEVEDLAGVVEALEANADIIMLDNMAPELMKEAVRMIGSRAL---VEAS 240 Query: 245 GNVSLETVRRIAETGVDVISVGRLTYSFSSLDISLDLNAKK 285 G VS E + IA+TGV +IS+G LT+S +LDISLD+ A K Sbjct: 241 GGVSEEALVEIAKTGVHLISIGALTHSVKALDISLDVEAIK 281 >Q8GE44.1 Q8GE44_HELMO Nicotinate-nucleotide pyrophosphorylase EC=2.4.2.19 (Fragment) Length = 288 Score = 300 bits (768), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 154/281 (54%), Positives = 200/281 (71%), Gaps = 3/281 (1%) Query: 5 YNEELVQTIKGWLHEDVGSGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVFEVVD 64 ++ E+ + + L ED+G+GD+TT +PA ++GIIHAKE G+I G+P+A+ VFE VD Sbjct: 4 FDPEIRELVTRALREDIGNGDLTTMNLVPADAYTRGIIHAKEAGVIAGLPIAQRVFETVD 63 Query: 65 PSLVFTALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLE 124 PSL F A+V+EG V KGT+IAEV GS ++L GER+ALN +QRLSG+A++T V+++ Sbjct: 64 PSLRFEAMVEEGDKVEKGTVIAEVFGSARSVLIGERVALNFLQRLSGIATKTARCVEQVA 123 Query: 125 GLPSRLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRA 184 +R+VDTRKTTPG R +EKYAVR+GGG NHRFGL+D V+IKDNHIK AG I QAV +A Sbjct: 124 YYQARIVDTRKTTPGLRAVEKYAVRIGGGKNHRFGLFDGVLIKDNHIKMAGSITQAVSQA 183 Query: 185 RANIPHTMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEAS 244 R +PHT+ I AL A ADIIMLDNM P++M EAVR I A EAS Sbjct: 184 RQAVPHTVKIEVEVEDLAGVAEALEAKADIIMLDNMVPDVMKEAVRMIGGNAL---VEAS 240 Query: 245 GNVSLETVRRIAETGVDVISVGRLTYSFSSLDISLDLNAKK 285 G VS E++ IA+TGV +IS+G LT+S SLDISLD+ K Sbjct: 241 GGVSEESLVEIAKTGVHLISMGSLTHSIKSLDISLDVEEIK 281 >A5D5U1.1 A5D5U1_PELTS Nicotinate-nucleotide pyrophosphorylase Length = 281 Score = 296 bits (759), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 157/277 (56%), Positives = 191/277 (68%), Gaps = 3/277 (1%) Query: 8 ELVQTIKGWLHEDVGSGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVFEVVDPSL 67 EL + I+ L ED+G+GD+TT + +P S G I AKEDG++ G+PVAE VF +DP + Sbjct: 7 ELNRLIERCLREDIGTGDLTTNSIVPPDAVSGGYILAKEDGVVAGLPVAEGVFLRLDPFV 66 Query: 68 VFTALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLEGLP 127 F ALV++G+ + G ++AE+ G AILTGERLALN +QRLSG+A+RT V+ + G Sbjct: 67 EFRALVRDGERIKSGQVLAELTGRARAILTGERLALNFLQRLSGIATRTARLVELIAGEK 126 Query: 128 SRLVDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRARAN 187 +RLVDTRKTTPG RMLEKYAVRVGGG NHRFGLYDAV+IKDNHIK AGGIR AV AR Sbjct: 127 ARLVDTRKTTPGLRMLEKYAVRVGGGYNHRFGLYDAVLIKDNHIKAAGGIRAAVEAARRG 186 Query: 188 IPHTMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEASGNV 247 PHT I AL AGADIIMLDNM P M EAV I +A EASG + Sbjct: 187 APHTARIEVEVEDLAGVEEALQAGADIIMLDNMDPAAMKEAVALINGRA---LVEASGRI 243 Query: 248 SLETVRRIAETGVDVISVGRLTYSFSSLDISLDLNAK 284 S E +R +A GVD+IS G LT+S SLDISL+L + Sbjct: 244 SEENIRAVAAAGVDLISAGALTHSVKSLDISLELRGQ 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,554,488,427 Number of extensions: 59145829 Number of successful extensions: 169767 Number of sequences better than 1.0e-05: 1422 Number of HSP's gapped: 166907 Number of HSP's successfully gapped: 1434 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)