BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0007.p (312 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K900.1 B3K900_9BACI Quinolinate synthetase complex, A subunit ... 572 e-161 B1DIE4.1 B1DIE4_9BACL Quinolinate synthetase complex, A subunit ... 545 e-153 C0ZHG2.1 C0ZHG2_BREBN Quinolinate synthetase A 478 e-133 B0TBP7.1 B0TBP7_HELMI Quinolinate synthetase complex, a subunit 365 2e-99 Q3A8D7.1 Q3A8D7_PELCD Quinolinate synthetase A 358 5e-97 >B3K900.1 B3K900_9BACI Quinolinate synthetase complex, A subunit EC=2.1.1.63 Length = 312 Score = 572 bits (1474), Expect = e-161, Method: Compositional matrix adjust. Identities = 270/312 (86%), Positives = 289/312 (92%) Query: 1 VEALALERKQEQNRELRVRLEQLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAE 60 +EALALERK EQNRELR RL QLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAA+ Sbjct: 1 MEALALERKAEQNRELRERLMQLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAQ 60 Query: 61 TDADVIVFCGVHFMGESAKILAPNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVV 120 TDA+VIVFCGVHFMGESAKILAPNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVV Sbjct: 61 TDAEVIVFCGVHFMGESAKILAPNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVV 120 Query: 121 TYINSSAEIKAETDICCTSANAVRVIESLDAEEIIWVPDKNLGQYVQDQTGKKLIIWEGY 180 TYINSSAEIKAETDICCTS+NAV+VI+S+D++EIIWVPDKNLG YVQ T KKLIIWEGY Sbjct: 121 TYINSSAEIKAETDICCTSSNAVKVIQSVDSDEIIWVPDKNLGHYVQQHTDKKLIIWEGY 180 Query: 181 CNTHDMLTVKDVVEMRAKYPEAEFVVHPECRPEVVAMGDYVGSTTSIIDYCKKSDRKQFI 240 CNTHDMLTVKDVVEM+AKYP+AEFVVHPECRPEVVAMGD+VGSTT+I++YCK+S K+FI Sbjct: 181 CNTHDMLTVKDVVEMKAKYPQAEFVVHPECRPEVVAMGDFVGSTTAILEYCKQSSAKEFI 240 Query: 241 VGTEDGTGYQLRKDSPDKEFHFATKFLVCPNMXXXXXXXXXXCLETMKPQIYVPPAVADK 300 VGTEDGTGYQLR DSPDK FHFATKFLVCPNM LETMKPQIYVPP VA+K Sbjct: 241 VGTEDGTGYQLRLDSPDKSFHFATKFLVCPNMKVNNLKKLVKALETMKPQIYVPPVVAEK 300 Query: 301 ARTSLERMLQVR 312 ARTSLERMLQV+ Sbjct: 301 ARTSLERMLQVK 312 >B1DIE4.1 B1DIE4_9BACL Quinolinate synthetase complex, A subunit EC=2.1.1.63 Length = 312 Score = 545 bits (1403), Expect = e-153, Method: Compositional matrix adjust. Identities = 257/312 (82%), Positives = 281/312 (90%) Query: 1 VEALALERKQEQNRELRVRLEQLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAE 60 +EALALERK EQNRELR RL QLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAA+ Sbjct: 1 MEALALERKAEQNRELRERLMQLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAQ 60 Query: 61 TDADVIVFCGVHFMGESAKILAPNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVV 120 TDADVIVFCGVHFMGESAKILAPNKTV+IPDERAGCPMADMVNVDGLRKLKAQHPNA VV Sbjct: 61 TDADVIVFCGVHFMGESAKILAPNKTVIIPDERAGCPMADMVNVDGLRKLKAQHPNATVV 120 Query: 121 TYINSSAEIKAETDICCTSANAVRVIESLDAEEIIWVPDKNLGQYVQDQTGKKLIIWEGY 180 TYINSSAEIKAETDICCTSANAV+V+ S++ +E+IWVPDKNLG YVQ T KK+IIWEGY Sbjct: 121 TYINSSAEIKAETDICCTSANAVKVVNSVEGDEVIWVPDKNLGHYVQQNTDKKMIIWEGY 180 Query: 181 CNTHDMLTVKDVVEMRAKYPEAEFVVHPECRPEVVAMGDYVGSTTSIIDYCKKSDRKQFI 240 CNTHDMLTVKDV EM+AKYP A+FVVHPECRPEVV +GD+VGSTT+II YCK+SD ++FI Sbjct: 181 CNTHDMLTVKDVEEMKAKYPNAQFVVHPECRPEVVELGDFVGSTTAIIKYCKESDCQEFI 240 Query: 241 VGTEDGTGYQLRKDSPDKEFHFATKFLVCPNMXXXXXXXXXXCLETMKPQIYVPPAVADK 300 VGTEDGTGYQLR DSP+K FHFA+K+LVCPNM CLETM+PQIYVP VAD+ Sbjct: 241 VGTEDGTGYQLRLDSPNKTFHFASKYLVCPNMKVNNLKKLVKCLETMQPQIYVPDHVADE 300 Query: 301 ARTSLERMLQVR 312 AR SLERML V+ Sbjct: 301 ARKSLERMLLVK 312 >C0ZHG2.1 C0ZHG2_BREBN Quinolinate synthetase A Length = 314 Score = 478 bits (1230), Expect = e-133, Method: Compositional matrix adjust. Identities = 226/311 (72%), Positives = 261/311 (83%) Query: 1 VEALALERKQEQNRELRVRLEQLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAE 60 ++ALALE+K ++N ELR RL QLKKERNAIILAH+YQR EIQEVADF GDSF LAQKA E Sbjct: 1 MDALALEKKAQRNAELRERLLQLKKERNAIILAHFYQRPEIQEVADFIGDSFGLAQKAKE 60 Query: 61 TDADVIVFCGVHFMGESAKILAPNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVV 120 TDADVI+FCGVHFMGESAKIL P KTV+IPDERAGCPMADMVNVDGLRKLKAQHPNAKVV Sbjct: 61 TDADVILFCGVHFMGESAKILNPQKTVIIPDERAGCPMADMVNVDGLRKLKAQHPNAKVV 120 Query: 121 TYINSSAEIKAETDICCTSANAVRVIESLDAEEIIWVPDKNLGQYVQDQTGKKLIIWEGY 180 YIN+SA++KAET ICCTS+NA RVIES+D++EIIWVPDKNLG YV T KK+IIWEGY Sbjct: 121 AYINTSADVKAETYICCTSSNAKRVIESIDSDEIIWVPDKNLGHYVSQFTDKKMIIWEGY 180 Query: 181 CNTHDMLTVKDVVEMRAKYPEAEFVVHPECRPEVVAMGDYVGSTTSIIDYCKKSDRKQFI 240 CNTHD L+V+D++ ++ ++PEA VVHPECRPEVV++ DYVGSTT I+ YC++S K+FI Sbjct: 181 CNTHDQLSVEDIMTLKQQHPEAVVVVHPECRPEVVSLADYVGSTTGILKYCRESSHKEFI 240 Query: 241 VGTEDGTGYQLRKDSPDKEFHFATKFLVCPNMXXXXXXXXXXCLETMKPQIYVPPAVADK 300 +GTEDGT Y L KDSPDK F FA+K+LVCPNM LE MKP+I+VP VAD Sbjct: 241 IGTEDGTRYMLEKDSPDKTFIFASKYLVCPNMKVNNLKKCVEALENMKPEIFVPEDVADA 300 Query: 301 ARTSLERMLQV 311 AR SLERML V Sbjct: 301 ARASLERMLAV 311 >B0TBP7.1 B0TBP7_HELMI Quinolinate synthetase complex, a subunit Length = 298 Score = 365 bits (938), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 168/291 (57%), Positives = 219/291 (75%), Gaps = 1/291 (0%) Query: 22 QLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAETDADVIVFCGVHFMGESAKIL 81 +LKKERNA+ILAH YQR E+Q+VADF GDS LAQ+AA+TDADVIVFCGVHFM ESA IL Sbjct: 7 KLKKERNAVILAHVYQRPEVQDVADFVGDSLQLAQQAAKTDADVIVFCGVHFMAESAAIL 66 Query: 82 APNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVVTYINSSAEIKAETDICCTSAN 141 +PNK VL+P+E AGCPMADMV D LR+ KA+ P +VV Y+N+SA +KAE+DICCTSAN Sbjct: 67 SPNKIVLLPEENAGCPMADMVTADALRRRKAEIPGVQVVAYVNTSAAVKAESDICCTSAN 126 Query: 142 AVRVIESLDAEE-IIWVPDKNLGQYVQDQTGKKLIIWEGYCNTHDMLTVKDVVEMRAKYP 200 A++V+ +L + I++VPD+NLG ++ QTG+++ WEGYCNTHD L+V D+ +A +P Sbjct: 127 ALKVVSALPEDRPILFVPDRNLGDWITRQTGREMHCWEGYCNTHDHLSVDDIERAKAAHP 186 Query: 201 EAEFVVHPECRPEVVAMGDYVGSTTSIIDYCKKSDRKQFIVGTEDGTGYQLRKDSPDKEF 260 +AE +VHPECRPEVVA D+V STT +I Y +S RK FI+ TE+G + LRK +P+K F Sbjct: 187 DAEVLVHPECRPEVVAKADFVASTTGLIRYALESPRKSFIIATEEGVLHPLRKQAPEKTF 246 Query: 261 HFATKFLVCPNMXXXXXXXXXXCLETMKPQIYVPPAVADKARTSLERMLQV 311 H A + L+CPNM L+ M+P+I V P + +KA SLERML + Sbjct: 247 HLAARTLICPNMKSTSLEKVVWSLQEMQPRITVDPVIREKALKSLERMLAI 297 >Q3A8D7.1 Q3A8D7_PELCD Quinolinate synthetase A Length = 304 Score = 358 bits (918), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 167/300 (55%), Positives = 215/300 (71%) Query: 12 QNRELRVRLEQLKKERNAIILAHYYQRDEIQEVADFRGDSFLLAQKAAETDADVIVFCGV 71 +++L+ + +L KERNA+I+AH+YQRDEIQE+AD GDS +A +AA+TD VIV CGV Sbjct: 2 NSQQLKEEIRRLAKERNALIMAHFYQRDEIQEIADVAGDSLAMAIEAAQTDCKVIVLCGV 61 Query: 72 HFMGESAKILAPNKTVLIPDERAGCPMADMVNVDGLRKLKAQHPNAKVVTYINSSAEIKA 131 HFM ESA ILAP+KTVL+P AGCPMADMV +D LR++KA+ P VVTY+NSSA IKA Sbjct: 62 HFMAESAAILAPDKTVLLPRTDAGCPMADMVEIDKLREMKARLPGRPVVTYVNSSAAIKA 121 Query: 132 ETDICCTSANAVRVIESLDAEEIIWVPDKNLGQYVQDQTGKKLIIWEGYCNTHDMLTVKD 191 E+DICCTSANAV V+ SLD +E+I VPD+NLG+Y+ T KK WEGYC HD L +D Sbjct: 122 ESDICCTSANAVAVVNSLDEDEVILVPDRNLGRYIAANTDKKCYFWEGYCPFHDQLPEED 181 Query: 192 VVEMRAKYPEAEFVVHPECRPEVVAMGDYVGSTTSIIDYCKKSDRKQFIVGTEDGTGYQL 251 V+ + +P+A F+ HPECRP+++AM D V ST+ +IDY + S K+FI+GTE G Y+L Sbjct: 182 VLAAKQAHPDALFLAHPECRPQILAMADLVASTSGMIDYVRTSPAKKFILGTEQGILYRL 241 Query: 252 RKDSPDKEFHFATKFLVCPNMXXXXXXXXXXCLETMKPQIYVPPAVADKARTSLERMLQV 311 RK++PDK+F L C M CL+TM P + VP +AD AR SLERML V Sbjct: 242 RKENPDKQFIMPNSLLFCKTMKYITLEEVLHCLQTMSPTVTVPAEIADGARKSLERMLAV 301 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,671,733,248 Number of extensions: 63817558 Number of successful extensions: 184428 Number of sequences better than 1.0e-05: 1125 Number of HSP's gapped: 180608 Number of HSP's successfully gapped: 1133 Length of query: 312 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 177 Effective length of database: 1,788,859,178 Effective search space: 316628074506 Effective search space used: 316628074506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)