BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0003.p (312 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8Z6.1 B3K8Z6_9BACI Serine/threonine protein kinase 318 4e-85 B1DIF4.1 B1DIF4_9BACL Serine/threonine protein kinase 235 4e-60 C1PDR5.1 C1PDR5_BACCO Serine/threonine protein kinase 159 2e-37 A7Z0I9.1 A7Z0I9_BACA2 YabT 156 2e-36 B7GFJ3.1 B7GFJ3_ANOFW Serine/threonine protein kinase 156 2e-36 >B3K8Z6.1 B3K8Z6_9BACI Serine/threonine protein kinase Length = 306 Score = 318 bits (815), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 165/313 (52%), Positives = 201/313 (64%), Gaps = 18/313 (5%) Query: 5 SNPPFPAGTVIRGKWRGNRYVVERMLGRGANGTVYLVQREGRRERYALKIGNDTLELQSE 64 SNP +P GTVI+GKWR +RY+VERMLG+GANG VYLV R G RERYALK+G D L+LQSE Sbjct: 5 SNPGYPPGTVIKGKWRNSRYIVERMLGKGANGKVYLVHRAGVRERYALKVGYDALDLQSE 64 Query: 65 INVLTSLQSSRKRKEHRAHRESALSSYLLESDDYKD---SVPFYVMRYVEGRSLHYFLAK 121 INVL +L +E YL+E+DD+ D V FYVMRY+EG LH+F+ K Sbjct: 65 INVLNAL------------KEQMTDPYLVEADDHHDGSGEVSFYVMRYIEGSPLHHFIRK 112 Query: 122 NGASWXXXXXXXXXXXXXXXHECGFVFGDLKPENVLVSNYGEAELIDYGGASPLGRSVKQ 181 G W H G+ FGDLKP NV+VS YG ELIDYGG S GRSVKQ Sbjct: 113 RGTEWVGLIGLKLLEKLSVLHASGYAFGDLKPNNVMVSAYGRVELIDYGGVSSFGRSVKQ 172 Query: 182 FTEWHDRGYWNAGSRTGDENYDLFAFAVLCLRLLNEEGLKAAAAQLPQTRSADELVKLAR 241 FTEW+DRGYWNAGSRTGD +YDLF+FAV+C+ LL+E+ LK A+ QLPQTRS +L + R Sbjct: 173 FTEWYDRGYWNAGSRTGDVSYDLFSFAVMCIGLLDEKSLKEASCQLPQTRSVADLQLVLR 232 Query: 242 SLPD-KKLSSWLCLALKGGFPGSEAATEAWKIHIYSTRKPGKETMA--TPGWLKSAFALS 298 P K + WL AL G F S A W+ IY G + TP WL++AF LS Sbjct: 233 RNPVFKPYAVWLNKALSGEFADSREALNEWRKRIYEGSSRGGRPVPGRTPRWLRNAFGLS 292 Query: 299 LFVLIFMIYYVFR 311 L ++ +Y+ FR Sbjct: 293 LVIMACAVYFFFR 305 >B1DIF4.1 B1DIF4_9BACL Serine/threonine protein kinase Length = 305 Score = 235 bits (600), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 131/305 (42%), Positives = 177/305 (58%), Gaps = 19/305 (6%) Query: 12 GTVIRGKWRGNRYVVERMLGRGANGTVYLVQREGRRERYALKIGNDTLELQSEINVLTSL 71 G V+ GKW+ +RY VER+LG GANG VYLV + R ALK+G D ++LQSEINVL +L Sbjct: 12 GAVVTGKWKQSRYRVERLLGEGANGKVYLVHAD--RGWCALKVGMDAIDLQSEINVLQTL 69 Query: 72 QSSRKRKEHRAHRESALSSYLLESDDY----KDSVPFYVMRYVEGRSLHYFLAKNGASWX 127 + +++ +YL E DD PFY+MRYV G ++ FL KNG W Sbjct: 70 EKQDRKQ-----------TYLYEVDDLLGEGGKEYPFYIMRYVRGVTISEFLEKNGPEWF 118 Query: 128 XXXXXXXXXXXXXXHECGFVFGDLKPENVLVSNYGEAELIDYGGASPLGRSVKQFTEWHD 187 H G+VFGDLK +NVLV++YG EL+DYGG + +G+ ++QFTE +D Sbjct: 119 PLIGLNLLGKLAELHRRGWVFGDLKIDNVLVADYGYVELVDYGGVTAMGKGIRQFTEIYD 178 Query: 188 RGYWNAGSRTGDENYDLFAFAVLCLRLLNEEGLKAAAAQLPQTRSADELVKLARSLPD-K 246 RG+WNAGSR+ D YDLF+FAVLC+ L + L A LPQ RS +EL +L Sbjct: 179 RGFWNAGSRSADARYDLFSFAVLCIMLHEPKKLHALTGGLPQIRSTEELERLISECKGLA 238 Query: 247 KLSSWLCLALKGGFPGSEAATEAWKIHIYSTRKPGKETMATPGWLKSAFALSLFVLIFMI 306 ++ WL AL G F + EAW+ + R ++ TPGWLK A+S VL + Sbjct: 239 PVAGWLKQALGGQFFDASEGAEAWR-RLLHRRDTRVKSAKTPGWLKGLAAVSAVVLGISV 297 Query: 307 YYVFR 311 Y++ R Sbjct: 298 YWLLR 302 >C1PDR5.1 C1PDR5_BACCO Serine/threonine protein kinase Length = 329 Score = 159 bits (403), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 95/233 (40%), Positives = 129/233 (55%), Gaps = 18/233 (7%) Query: 8 PF--PAGTVIRGKWRGNRYVVERMLGRGANGTVYLVQREGRRERYALKIGNDTLELQSEI 65 PF P GTV+ GKW NRY + + LG GANG VYL E ALK+G T ++ SE Sbjct: 10 PFNDPPGTVVTGKWHKNRYRLLKRLGAGANGAVYLA--ESAAGPVALKVGVSTAQVASES 67 Query: 66 NVLTSLQSSRKRKEHRAHRESALSSYLLESDDY---KDSVPFYVMRYVEGRSLHYFLAKN 122 +VL ++ +R + +L LE+DD+ ++ +PFYVM Y++G L F+ Sbjct: 68 SVLKTISKARGQ---------SLGPSFLEADDWERGREIIPFYVMEYIDGPDLLSFVTGK 118 Query: 123 GASWXXXXXXXXXXXXXXXHECGFVFGDLKPENVLVSNYGE-AELIDYGGASPLGRSVKQ 181 G SW HE G++FGDLKPEN+LV G ID GG + +GRSVK+ Sbjct: 119 GFSWAAVLVIQLLSDLQHLHEQGWIFGDLKPENLLVGTPGNRIRCIDPGGMTKIGRSVKE 178 Query: 182 FTEWHDRGYWNAGSRTGDENYDLFAFAVLCLRL-LNEEGLKAAAAQLPQTRSA 233 FTE+ DRGYW GSR + +YDLF+ A++ + L LK A PQ +A Sbjct: 179 FTEFFDRGYWGLGSRKAEPSYDLFSVAMVMINLAYPNRFLKKQANGFPQLMAA 231 >A7Z0I9.1 A7Z0I9_BACA2 YabT Length = 333 Score = 156 bits (395), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 91/260 (35%), Positives = 136/260 (52%), Gaps = 22/260 (8%) Query: 12 GTVIRGKWRGNRYVVERMLGRGANGTVYLVQREGRRERYALKIGNDTLELQSEINVLTSL 71 GT I+GKW G Y + + LG+GANG VYL + G + ALKI +D++ + SE+NVL S Sbjct: 16 GTAIQGKWNGKSYRLLKQLGKGANGIVYLAEASGGQ--VALKISSDSMSVTSEVNVLKSF 73 Query: 72 QSSRKRKEHRAHRESALSSYLLESDD-----YKDSVPFYVMRYVEGRSLHYFLAKNGASW 126 +R + + ++DD +V FYVM Y+EG L +++ GA W Sbjct: 74 SKARSQ---------TMGPSFFDADDAFIPQLNKNVSFYVMEYIEGPLLPQYISSKGAEW 124 Query: 127 XXXXXXXXXXXXXXXHECGFVFGDLKPENVLVSNYGEA-ELIDYGGASPLGRSVKQFTEW 185 H+ G+VFGDLKP+N++V+ A ID GG + GR++K++TE+ Sbjct: 125 IPVLMVQLLSSLSVLHQQGWVFGDLKPDNLIVTGPPAAIRCIDVGGTTKQGRAIKEYTEF 184 Query: 186 HDRGYWNAGSRTGDENYDLFAFAVLCLRLLNEEGLKAAAAQLPQTRSADELVKLARSLPD 245 +DRGYW G+R + +YDLFA A++ + ++ K + Q RS E Sbjct: 185 YDRGYWGYGTRKAEPSYDLFAVAMIMIHCAQKKECKKSGQPKEQLRSIIEGHPFLN---- 240 Query: 246 KKLSSWLCLALKGGFPGSEA 265 K L AL G +P +EA Sbjct: 241 -KYKKVLFSALNGQYPSAEA 259 >B7GFJ3.1 B7GFJ3_ANOFW Serine/threonine protein kinase Length = 318 Score = 156 bits (395), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 92/212 (43%), Positives = 120/212 (56%), Gaps = 17/212 (8%) Query: 9 FPAGTVIRGKWRGNRYVVERMLGRGANGTVYLVQREGRRERYALKIGNDTLELQSEINVL 68 P GTVI+GKW RY + + LG+GANG VYL REGR ALKI N + SE+NVL Sbjct: 15 IPTGTVIQGKWHKRRYEMIKQLGKGANGVVYLA-REGR-TFVALKISNQATAMMSEVNVL 72 Query: 69 TSLQSSRKRKEHRAHRESALSSYLLESDDY-----KDSVPFYVMRYVEGRSLHYFLAKNG 123 R+ E + AL LL+ DD+ K FYVM Y+EG L F+ K G Sbjct: 73 ------RRFSEVQG---VALGPCLLDVDDWFHPQEKRIYSFYVMEYIEGDDLLTFIQKRG 123 Query: 124 ASWXXXXXXXXXXXXXXXHECGFVFGDLKPENVLVS-NYGEAELIDYGGASPLGRSVKQF 182 W HE G+VFGDLKPEN++VS + LID GG + GR++K+F Sbjct: 124 KDWIIVIILQLLGDLHKLHEKGWVFGDLKPENLIVSGTVPKVRLIDVGGTTIRGRAIKEF 183 Query: 183 TEWHDRGYWNAGSRTGDENYDLFAFAVLCLRL 214 TE+ DRGYW G+R + +YDLFA A++ + + Sbjct: 184 TEFFDRGYWGMGTRKAEPSYDLFAVAMIMINM 215 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,849,523,563 Number of extensions: 73068652 Number of successful extensions: 249678 Number of sequences better than 1.0e-05: 87 Number of HSP's gapped: 251508 Number of HSP's successfully gapped: 87 Length of query: 312 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 177 Effective length of database: 1,788,859,178 Effective search space: 316628074506 Effective search space used: 316628074506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)