BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0055.0006.p (265 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8XWZ7.1 Q8XWZ7_RALSO Probable short-chain dehydrogenase/reducta... 254 8e-66 Q0RSP9.1 Q0RSP9_FRAAA Putative dehydrogenase/reductase oxidoredu... 252 2e-65 Q2JG54.1 Q2JG54_FRASC Short-chain dehydrogenase/reductase SDR 250 1e-64 A8LHI7.1 A8LHI7_FRASN Short-chain dehydrogenase/reductase SDR 248 3e-64 A8YHH6.1 A8YHH6_MICAE Genome sequencing data, contig C313 242 3e-62 >Q8XWZ7.1 Q8XWZ7_RALSO Probable short-chain dehydrogenase/reductase sdr; oxidoreductase protein EC=1.-.-.- Length = 275 Score = 254 bits (648), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 131/262 (50%), Positives = 170/262 (64%), Gaps = 1/262 (0%) Query: 2 NSYHYLGKVALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAY 61 N + + GK ALITGASSGIG +A LA RG ++L ARS+DKL L +E+ R + A Sbjct: 3 NCFDFRGKTALITGASSGIGREFAYALAKRGAKLLLVARSRDKLHDLTAELRRDYACDAD 62 Query: 62 ALPIDLSKAEAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAAL 121 L +DLS +A +A + G VD+LINNAGF T GRFE I R+++++++N A Sbjct: 63 FLTVDLSAPDAIPTVAHLLKATGTVVDVLINNAGFATYGRFETIPWTRQRDEVLVNCMAA 122 Query: 122 VELTHLFLPGMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGV 181 +ELTHL LPGM R DG V+NVAS AAF P Y+A+YGATKAF+LSFSEA+WAE R RG+ Sbjct: 123 IELTHLLLPGMQARSDGAVINVASTAAFQPDPYMAIYGATKAFLLSFSEAVWAENRHRGI 182 Query: 182 RVLALCPGATDTGFFDAVGSRDMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTT 241 RVLALCPGAT TGFFD VG+++ + VVQ +DRN Y I G N + Sbjct: 183 RVLALCPGATQTGFFDVVGAKEAAVGTPMPV-ASVVQDALWALDRNRSYRIVGTQNRLLA 241 Query: 242 LICRILPRRSLALINERNSRPK 263 + R+L R A + E+ RP+ Sbjct: 242 NLQRLLSREMSARLVEKILRPR 263 >Q0RSP9.1 Q0RSP9_FRAAA Putative dehydrogenase/reductase oxidoreductase EC=1.-.-.- Length = 271 Score = 252 bits (644), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 123/248 (49%), Positives = 174/248 (70%), Gaps = 1/248 (0%) Query: 11 ALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPIDLSKA 70 AL+TGASSGIG +A+ AARG ++L A ++ +L +A + +GV+ + +PIDL++ Sbjct: 4 ALVTGASSGIGAAFAQAYAARGATLVLVAPAEPRLTEVAQRLGTRYGVEVHTVPIDLAEE 63 Query: 71 EAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELTHLFLP 130 P LAK+++E G+ +DIL+NNAGFGT G + +++P+R+ ++M+N A+ + HLFLP Sbjct: 64 SGPDQLAKAVAETGLEIDILVNNAGFGTRGPYHELSPDRDHREVMVNVVAVERVAHLFLP 123 Query: 131 GMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLALCPGA 190 M+ R G ++NV+S + F Y+AVYGA+KAFVLSFS LWAE R RGVRVLALCPG Sbjct: 124 AMVARGSGTIINVSSTSGFQAVPYMAVYGASKAFVLSFSIGLWAECRPRGVRVLALCPGP 183 Query: 191 TDTGFFDAVGSRDMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTTLICRILPRR 250 TDT FF+ +GSR M L T ++VV A FR +DR Y+IDGR+NY+T+ R+LPR Sbjct: 184 TDTDFFEVLGSR-MSAGGRLRTTDEVVAAAFRALDRGQAYVIDGRLNYLTSNASRLLPRG 242 Query: 251 SLALINER 258 +A I ER Sbjct: 243 LVARITER 250 >Q2JG54.1 Q2JG54_FRASC Short-chain dehydrogenase/reductase SDR Length = 291 Score = 250 bits (638), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 122/252 (48%), Positives = 171/252 (67%), Gaps = 1/252 (0%) Query: 11 ALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPIDLSKA 70 ALITGASSGIG +A+ A RG ++L A S+ +L +A + +G +A+ + +DL+ Sbjct: 12 ALITGASSGIGAAFAEAYADRGARLVLVAPSEPRLWMVAERLGARYGTEAHVIAVDLADE 71 Query: 71 EAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELTHLFLP 130 P LA++++E G+ VDIL+NNAGFGT G + +++ +R+ ++MLN A+ + HLFLP Sbjct: 72 RGPDRLAEAVAEAGLEVDILVNNAGFGTRGPYHELSADRDHREVMLNVVAVERVAHLFLP 131 Query: 131 GMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLALCPGA 190 GM+ R G V+NV+S + F Y+ VYGA+KAFVLSFS LWAE R RGVRVLALCPG Sbjct: 132 GMVARGRGTVINVSSTSGFQAVPYMTVYGASKAFVLSFSIGLWAEYRRRGVRVLALCPGP 191 Query: 191 TDTGFFDAVGSRDMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTTLICRILPRR 250 TDT FF+ +G+R M L T ++V+ FR +DR Y+IDGR+NY+T+ R+LPR Sbjct: 192 TDTEFFEVLGTR-MSAGGRLRTTDEVIATAFRALDRGQPYVIDGRLNYLTSNASRLLPRG 250 Query: 251 SLALINERNSRP 262 +A I ER RP Sbjct: 251 LVARITERVLRP 262 >A8LHI7.1 A8LHI7_FRASN Short-chain dehydrogenase/reductase SDR Length = 263 Score = 248 bits (634), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 124/260 (47%), Positives = 178/260 (68%), Gaps = 1/260 (0%) Query: 6 YLGKVALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPI 65 + + AL+TGASSGIG +A+ LAARG ++L A S++KL +A + HGV+ + +P+ Sbjct: 4 FRNRTALVTGASSGIGAAFAEALAARGADLVLAAPSEEKLRPVAERLRALHGVRVWMVPV 63 Query: 66 DLSKAEAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELT 125 DL++ + P LL + + G +D+L+NNAGFGT GR+E++ +R+ ++M++ A+ +T Sbjct: 64 DLAREDGPDLLVEQVRATGREIDVLVNNAGFGTYGRYEELPADRDHREVMVDVVAVERVT 123 Query: 126 HLFLPGMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLA 185 HL LP M+ R G VVNV+S + F Y+AVYGA+KAFVLSFS ALWAE R RG+RVLA Sbjct: 124 HLLLPAMVERGRGTVVNVSSTSGFQAVPYMAVYGASKAFVLSFSIALWAEYRSRGIRVLA 183 Query: 186 LCPGATDTGFFDAVGSRDMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTTLICR 245 +CPG TDT FF+ +G R M L T ++VV A FRG+DR Y+IDGR+NY+T+ R Sbjct: 184 VCPGPTDTNFFEVLGRR-MSAGGRLRTTDEVVSAAFRGLDRGLPYVIDGRLNYLTSNASR 242 Query: 246 ILPRRSLALINERNSRPKAQ 265 +L R +A I R RP+ + Sbjct: 243 LLGRGLVAGITARTLRPRGR 262 >A8YHH6.1 A8YHH6_MICAE Genome sequencing data, contig C313 Length = 265 Score = 242 bits (617), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 121/264 (45%), Positives = 180/264 (68%), Gaps = 12/264 (4%) Query: 4 YHYLGKVALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYAL 63 ++Y GK ALITGASSGIGE++A+ELA RG ++IL AR+++KL +A+++ + +GV+ + Sbjct: 2 FNYRGKTALITGASSGIGEIFARELAKRGMNLILVARTEEKLRTIATDLYQVYGVRTEVI 61 Query: 64 PIDLSKAEAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVE 123 +DLS+ A + + ++ E +TVD+L+NNAGF FE I E+++ Q+M+N ALV+ Sbjct: 62 VVDLSRENAAQDVFSAVEEKALTVDLLVNNAGFLNYAPFEQIPLEQDRAQVMVNVVALVD 121 Query: 124 LTHLFLPGMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRV 183 LTH F+PGML + +G V+N++S AF P Y+AVYGA+KAFVLSFSEALWAE R RG+RV Sbjct: 122 LTHKFIPGMLAKGEGAVINLSSSGAFQPMPYMAVYGASKAFVLSFSEALWAEYRHRGLRV 181 Query: 184 LALCPGATDTGFFDAVGSRDMV---ENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINY-M 239 LALCPG T T R +V ++ + ++P++VV + ++ Y+I G NY + Sbjct: 182 LALCPGPTAT--------RSLVKIFDDGSATSPQRVVAVALKALEDGRNYVIPGLKNYWL 233 Query: 240 TTLICRILPRRSLALINERNSRPK 263 I R++PR I ER +RP+ Sbjct: 234 ANSIPRLMPRNITVQILERITRPR 257 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,460,819,722 Number of extensions: 57112113 Number of successful extensions: 233279 Number of sequences better than 1.0e-05: 27938 Number of HSP's gapped: 205523 Number of HSP's successfully gapped: 27981 Length of query: 265 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 132 Effective length of database: 1,807,701,208 Effective search space: 238616559456 Effective search space used: 238616559456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)