BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0013.p (505 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K504.1 B3K504_9BACI Carboxylesterase EC=3.1.1.1 463 e-128 A7Z924.1 A7Z924_BACA2 PnbA EC=3.1.1.1 409 e-112 Q9X6Z3.1 Q9X6Z3_BACSP Carboxylesterase EC=3.1.1.3 402 e-110 Q2B3Q6.1 Q2B3Q6_9BACI Para-nitrobenzyl esterase (Intracellular e... 394 e-108 Q65MY7.1 Q65MY7_BACLD PnbA 390 e-106 >B3K504.1 B3K504_9BACI Carboxylesterase EC=3.1.1.1 Length = 487 Score = 463 bits (1191), Expect = e-128, Method: Compositional matrix adjust. Identities = 238/487 (48%), Positives = 304/487 (62%), Gaps = 4/487 (0%) Query: 1 MTATLVTTLYGKLQGAEEEGVKVWRGIPFAAPPVGKLRFRAPQPPQPWDSVREALAFSPV 60 M+ +V T G++QG E GV+VW+GIP+A PPVG LRFR+P PP WD +RE AFSP+ Sbjct: 1 MSELVVQTKDGRIQGLTENGVRVWKGIPYAKPPVGPLRFRSPVPPDSWDGIRETTAFSPM 60 Query: 61 SHQP-ASTSSTRFGGLSPEYSEDCLYLNVWSPAEA-GESLPVMVWIHGGTFVTGAGSQPM 118 QP STSS GG++ SEDCLYLNVW+P E LPVMVWIHGG FVTG+GS P Sbjct: 61 CLQPLESTSSMLGGGVTKTVSEDCLYLNVWAPRNMPAEPLPVMVWIHGGAFVTGSGSLPS 120 Query: 119 FDGACMARNGKVVLVTVNYRLGPFGFLHLSPFGGGLASNAGLLDQIAALEWVQGNIAAFG 178 ++GA G V++VT+NYRLGP GFLHLSP+G +SN GLLDQIAALEWV+ NIAAFG Sbjct: 121 YNGAPFVLRGDVIVVTLNYRLGPLGFLHLSPYGETYSSNTGLLDQIAALEWVRDNIAAFG 180 Query: 179 GNPQRVTVFGESAGSMSIXXXXXXXXXXXXXXXXIMESGAAQSXXXXXXXXXXXXXXXXX 238 G+P+RVTVFGESAGSMSI IMESGAAQ Sbjct: 181 GDPERVTVFGESAGSMSIAALLAMPAAKGLFHRAIMESGAAQ-VMVPEQARMITAGLLKE 239 Query: 239 XXXXXXXXXXXDHLPAEQIMEAAARMTYKLSGEALSMFFQPVVEPATLPXXXXXXXXXXX 298 D L E I EA R+ ++ G +++M FQPV+EP TLP Sbjct: 240 LDITPDNIHQLDSLTGESIFEAGERLKKRI-GPSVNMIFQPVLEPDTLPHDPLQAVQSGW 298 Query: 299 XXXIPLLIGTNLHEGNLFFREERPGESFEQSLKSLELLMGIDDLAELAGGYPKSWEGQAA 358 +P+LIGTNL EG LFFR + P + ++++E++ GI+D + + YP S +G A Sbjct: 299 AKDVPILIGTNLDEGALFFRPDMPLVKEDVLVRTVEMMTGIEDASPITKEYPYSIDGHAQ 358 Query: 359 FLTDLFFWGSSIAFAESQSAAAPVWMYRFDWTVEGHPLLEKAVHGAEILYVFGNLHLLKQ 418 +TDL+FW S++ FA +Q APVWMYRFDWT+ HP+L KA+H EI +VF L L + Sbjct: 359 IMTDLYFWRSALQFASAQCKHAPVWMYRFDWTLPDHPVLGKAMHALEIPFVFNTLALFEN 418 Query: 419 YGLTVTPEMQRLSEVMQKTWTAFAHHGNPSLADLEWPQYRTDSRATLIFDGETRVVQDPD 478 G+ V Q LS+ MQ W AFA G+P L WP Y SR+T+IF+ ++R+++DPD Sbjct: 419 IGVQVDAATQALSDRMQDAWIAFARSGSPDTEALPWPAYDPVSRSTMIFNNDSRIIEDPD 478 Query: 479 ADKRRRL 485 A KR L Sbjct: 479 AKKREML 485 >A7Z924.1 A7Z924_BACA2 PnbA EC=3.1.1.1 Length = 482 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 213/489 (43%), Positives = 278/489 (56%), Gaps = 8/489 (1%) Query: 1 MTATLVTTLYGKLQGAEEEGVKVWRGIPFAAPPVGKLRFRAPQPPQPWDSVREALAFSPV 60 MT V T G L+G G + W+GIP+A PPVG+LRF+AP+PP PWD V+ A +F PV Sbjct: 1 MTKLTVQTRCGALKGTAGRGARTWKGIPYAKPPVGELRFKAPEPPAPWDGVKHADSFGPV 60 Query: 61 SHQPASTSSTRFGGLSPEYSEDCLYLNVWSPAEAGESLPVMVWIHGGTFVTGAGSQPMFD 120 QP S F G P SEDCLYLNV++P GE PVMVWIHGG F GAGS+P++D Sbjct: 61 CPQPDDMLSISFSGDVPPQSEDCLYLNVFAPDSEGEKKPVMVWIHGGAFYLGAGSEPLYD 120 Query: 121 GACMARNGKVVLVTVNYRLGPFGFLHLSPFGGGLASNAGLLDQIAALEWVQGNIAAFGGN 180 G+ +A +G V++VT+NYRLGPFGFLHLS ++N GLLDQIAAL WV+ NI+ FGG+ Sbjct: 121 GSALAADGDVIVVTLNYRLGPFGFLHLSSIHDTYSANIGLLDQIAALRWVKDNISEFGGD 180 Query: 181 PQRVTVFGESAGSMSIXXXXXXXXXXXXXXXXIMESGAAQSXXXXXXXXXXXXXXXXXXX 240 P VT+FGESAG MSI I+ESGA+ + Sbjct: 181 PDNVTIFGESAGGMSIAALMAMPDAKGLFHKAILESGASHTIPADMAKDIAAAFIHEAGT 240 Query: 241 XXXXXXXXXDHLPAEQIMEAAARMTYKLSGEALSMFFQPVVEPATLPXXXXXXXXXXXXX 300 L +++ A ++ L + FQP ++PATLP Sbjct: 241 DQLQ------ELSVNDLLKTADKVRRSLDQNIFQLLFQPAIDPATLPAEPVKAIADGAAE 294 Query: 301 XIPLLIGTNLHEGNLFFREERPGESFEQSLKSLELLMGIDDLAELAGGYPKSWEGQAAFL 360 IP++IGTN E LFF + S ++ L +G + + A YP S +GQ + Sbjct: 295 GIPMIIGTNRDEAYLFFTPDTDIHSEKKQQDYLHYYLGENCTEQAADLYPHSLKGQIDMM 354 Query: 361 TDLFFWGSSIAFAESQSAAAPVWMYRFDWTVEGHPLLEKAVHGAEILYVFGNLH-LLKQY 419 TDL FW ++AFA+ QS APVWMYRFDW E P KAVH E+ +VFGN L K Sbjct: 355 TDLIFWRPAVAFAQGQSQHAPVWMYRFDWHGETPP-FHKAVHALELPFVFGNFDSLRKTL 413 Query: 420 GLTVTPEMQRLSEVMQKTWTAFAHHGNPSLADLEWPQYRTDSRATLIFDGETRVVQDPDA 479 + + ++LS+ +Q W AFA GNP+ + WP+Y TDSR TL+F+ +T V DPD+ Sbjct: 414 QEPLGDDAEQLSKQIQSAWLAFAKTGNPNTSHFNWPEYDTDSRETLLFNTDTAVESDPDS 473 Query: 480 DKRRRLFAA 488 KRR LF A Sbjct: 474 AKRRILFQA 482 >Q9X6Z3.1 Q9X6Z3_BACSP Carboxylesterase EC=3.1.1.3 Length = 485 Score = 402 bits (1032), Expect = e-110, Method: Compositional matrix adjust. Identities = 215/487 (44%), Positives = 289/487 (59%), Gaps = 6/487 (1%) Query: 1 MTATLVTTLYGKLQGAEEEGVKVWRGIPFAAPPVGKLRFRAPQPPQPWDSVREALAFSPV 60 M V T YGK+QG +G VW+GIP+A PPVG++RF+AP P+ WD +R+A F P Sbjct: 1 MRELQVQTKYGKVQGELLQGASVWKGIPYAKPPVGEMRFQAPTQPESWDGIRQATEFGPE 60 Query: 61 SHQPASTSSTRFGGLSPEYSEDCLYLNVWSP-AEAGESLPVMVWIHGGTFVTGAGSQPMF 119 + QP S GG P SED LYLN+W+P E+ LPVMVWIHG +FVTG+GS P++ Sbjct: 61 NIQP-RHDSEWMGGQKPPESEDSLYLNIWAPEKESSHPLPVMVWIHGASFVTGSGSLPVY 119 Query: 120 DGACMARNGKVVLVTVNYRLGPFGFLHLSPFGGGLASNAGLLDQIAALEWVQGNIAAFGG 179 DG +A G V++VT+NYRLGP GFLH++P G G SNAGLLDQ+AAL+WV+ NI AFGG Sbjct: 120 DGTQLAVRGDVIVVTINYRLGPLGFLHMAPLGEGYVSNAGLLDQVAALQWVKDNITAFGG 179 Query: 180 NPQRVTVFGESAGSMSIXXXXXXXXXXXXXXXXIMESGAAQSXXXXXXXXXXXXXXXXXX 239 +P +VTVFGESAGSMSI IMESGA+Q Sbjct: 180 DPNQVTVFGESAGSMSIAALMAMPAAKGLFQRAIMESGASQ-FMPAEQASALREGMLKVL 238 Query: 240 XXXXXXXXXXDHLPAEQIMEAAARMTYKLSGEALSMFFQPVVEPATLPXXXXXXXXXXXX 299 + +P EQIM AAA + + SG +++ FQPV++ TLP Sbjct: 239 GVDRDNLEKLNSIPVEQIM-AAAEVVKQQSGAGMALLFQPVLDGETLPQVPLQAVSEGSA 297 Query: 300 XXIPLLIGTNLHEGNLFFREERPGESFEQSLKSLELLM-GIDDLAELAGGYPKSWEGQAA 358 + +LIGT LHEG LF + P ++ + + +++ +A YPK+ +GQA Sbjct: 298 KDVSILIGTTLHEGALFIQPHVPYSKDIDMVQGVNFMTPDLENRVAIADSYPKTADGQAQ 357 Query: 359 FLTDLFFWGSSIAFAESQSAAAPVWMYRFDWTVEGHPLLEKAVHGAEILYVFGNLHLLKQ 418 +TD+FFW S++ +A +Q APVWMYRFDW + HPLL++A+H E+ +VF L LK Sbjct: 358 VMTDMFFWRSALQYAAAQQQHAPVWMYRFDWVMPEHPLLKRAIHSIEMFFVFNTLDALKF 417 Query: 419 YGLTVTPEMQRLSEVMQKTWTAFAHHGNPSLADLEWPQYRTDSRATLIFDGETRVVQDPD 478 + L+ +Q W AFA G PS+A ++WP+Y D RATLIF+ E VV DP+ Sbjct: 418 MKAEPDEAAKALALKVQDAWIAFAKDGKPSVAGIKWPEYSKD-RATLIFNHEIEVVHDPE 476 Query: 479 ADKRRRL 485 + KR L Sbjct: 477 SSKRELL 483 >Q2B3Q6.1 Q2B3Q6_9BACI Para-nitrobenzyl esterase (Intracellular esterase B) Length = 486 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 211/490 (43%), Positives = 282/490 (57%), Gaps = 13/490 (2%) Query: 1 MTATLVTTLYGKLQGAEEEGVKVWRGIPFAAPPVGKLRFRAPQPPQPWDSVREALAFSPV 60 M + +V T G ++G E VW+GIPFA P G+LRF+ P+PP+ W+ EA F PV Sbjct: 1 MDSAIVKTKQGFVRGTEGGAATVWKGIPFAKKPAGELRFQPPEPPEAWEGTLEANEFGPV 60 Query: 61 SHQPASTSSTRFGGLSPEYSEDCLYLNVWSPAEAGESLPVMVWIHGGTFVTGAGSQPMFD 120 Q ++ G + SEDCLYLN+W+PA E LPVMVWIHGG F GAGS P++D Sbjct: 61 CTQNKDIAAM-LGAPADNMSEDCLYLNIWAPAGPREGLPVMVWIHGGAFRAGAGSSPLYD 119 Query: 121 GACMARNGKVVLVTVNYRLGPFGFLHLSPFGGGLASNAGLLDQIAALEWVQGNIAAFGGN 180 G MA NG V++VT+NYRLG FGFLHL+ +N GLLDQ+AAL+WV+ NI AFGG+ Sbjct: 120 GTSMAENGGVIVVTINYRLGAFGFLHLAGVDSSYTANLGLLDQVAALKWVRENIEAFGGD 179 Query: 181 PQRVTVFGESAGSMSIXXXXXXXXXXXXXXXXIMESGAAQSXXXXXXXXXXXXXXXXXXX 240 P++VTVFGESAG+MSI I+ESGA+Q Sbjct: 180 PEQVTVFGESAGAMSIASLLAMPAAKGLFQKAILESGASQ-IIPPEQASMIAKAVLQQLG 238 Query: 241 XXXXXXXXXDHLPAEQIMEAAARMTYKLSGEALSMFFQPVVEPATLPXXXXXXXXXXXXX 300 + +PAE I++AA R+T +G+ M FQP V+ TLP Sbjct: 239 IDDHNMEKLNEVPAEDILKAAERVT---AGQGPGMIFQPTVDAETLPVPPLQAITGGAAE 295 Query: 301 XIPLLIGTNLHEGNLFFR---EERPGESFEQSLKSLELLMGIDDLAELAGGYPKSWEGQA 357 IP++IGTN EG FFR PG + ++++ L +G + ++ YP++ EG++ Sbjct: 296 GIPVMIGTNHDEGAFFFRPGSAPMPGPARDEAMAKL---IGPEAAEKVKRHYPETIEGES 352 Query: 358 AFLTDLFFWGSSIAFAESQSAAAPVWMYRFDWTVEGHPLLEKAVHGAEILYVFGNLHLLK 417 F+TD FW +I +A Q+ APVWMYRFDW + GHP + KA HG EI +VF NL Sbjct: 353 QFMTDFVFWKPAIEYAAGQTKHAPVWMYRFDWHIPGHPHVGKAAHGLEIAFVFSNLFYFS 412 Query: 418 QYGLTVTPEMQRLSEVMQKTWTAFAHHGNPSLA--DLEWPQYRTDSRATLIFDGETRVVQ 475 + G+ V +QRLS MQ W +FA GNP+ A D+ WP Y+ R T IFD E+RV Sbjct: 413 RLGVQVDETIQRLSRHMQSAWISFAKAGNPNAAGDDMNWPAYQPLDRYTYIFDSESRVEA 472 Query: 476 DPDADKRRRL 485 DP +KR + Sbjct: 473 DPYKEKRELI 482 >Q65MY7.1 Q65MY7_BACLD PnbA Length = 490 Score = 390 bits (1003), Expect = e-106, Method: Compositional matrix adjust. Identities = 214/489 (43%), Positives = 274/489 (56%), Gaps = 6/489 (1%) Query: 1 MTATLVTTLYGKLQGAEEEGVKVWRGIPFAAPPVGKLRFRAPQPPQPWDSVREALAFSPV 60 M T V T +GKL+G E GV++++G+P+A PPVG LRFR PQ + W+ +A F PV Sbjct: 1 MYDTTVETRFGKLKGRAENGVRIFKGVPYAKPPVGDLRFREPQRMEAWEGELDAFQFGPV 60 Query: 61 SHQPASTSSTRFGGLSPEYSEDCLYLNVWSPAEAGESLPVMVWIHGGTFVTGAGSQPMFD 120 QP G + SEDCLYLNV++P EA LPVMVWIHGG F GAGS+P++D Sbjct: 61 CPQPDGVLPESAG---VQKSEDCLYLNVYAPEEADGDLPVMVWIHGGAFYRGAGSEPLYD 117 Query: 121 GACMARNGKVVLVTVNYRLGPFGFLHLSPFGGGLASNAGLLDQIAALEWVQGNIAAFGGN 180 G +A+ GKV++VT+NYRLGPFGFLHLS +SN GLLDQIAALEWV+ NIA FGG+ Sbjct: 118 GTQLAKQGKVIVVTINYRLGPFGFLHLSSIDDSYSSNLGLLDQIAALEWVKDNIAFFGGD 177 Query: 181 PQRVTVFGESAGSMSIXXXXXXXXXXXXXXXXIMESGAAQSXXXXXXXXXXXXXXXXXXX 240 +TVFGESAGSMSI IMESGA+ + Sbjct: 178 RHHITVFGESAGSMSIASLLAMPKAKGLFQQAIMESGASATMSDKLAKAAAERFLRILDI 237 Query: 241 XXXXXXXXXDHLPAEQIMEAAARMTYKLSGEALSMFFQPVVEPATLPXXXXXXXXXXXXX 300 D + ++++EAA ++ + + F P ++ TLP Sbjct: 238 DHHHLERLHD-VSDQELLEAADQLRTLMGENIFELIFLPALDEKTLPLKPEVAVAKGAAK 296 Query: 301 XIPLLIGTNLHEGNLFFREERPGESFEQSLKSLELLMGIDDLAELAGGYPKSWEGQAAFL 360 I LLIGTN EG LFF + + + LE MG + + YP+S EG + Sbjct: 297 EINLLIGTNRDEGVLFFPSDSDLLPESKINEILEEYMGKEAAEAASSLYPRSLEGHVDMM 356 Query: 361 TDLFFWGSSIAFAESQSAAAPVWMYRFDWTVEG-HPLLEKAVHGAEILYVFGNLHLLKQY 419 TDL FW S+ FA +QS A V+MYRFDW + P KA HG EI +VFGN+ +L+Q Sbjct: 357 TDLIFWHPSVVFASAQSRYASVFMYRFDWHADSEQPPFNKAAHGLEIPFVFGNMDILEQL 416 Query: 420 -GLTVTPEMQRLSEVMQKTWTAFAHHGNPSLADLEWPQYRTDSRATLIFDGETRVVQDPD 478 G E Q L+E +Q W +FA GNPS D+ WP Y DSR TLIFD E V DP Sbjct: 417 TGTKAGEEAQLLAEQIQAAWVSFARSGNPSTDDVSWPDYDEDSRKTLIFDQEVAVESDPY 476 Query: 479 ADKRRRLFA 487 +DKR+ L A Sbjct: 477 SDKRKMLTA 485 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,830,936,544 Number of extensions: 118934835 Number of successful extensions: 275108 Number of sequences better than 1.0e-05: 3032 Number of HSP's gapped: 266940 Number of HSP's successfully gapped: 3247 Length of query: 505 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 365 Effective length of database: 1,741,754,103 Effective search space: 635740247595 Effective search space used: 635740247595 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)