BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0007.p (367 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3F903.1 A3F903_BACME GerUB 258 7e-67 A3F904.1 A3F904_BACME GerVB 255 4e-66 B7GJD3.1 B7GJD3_ANOFW Spore germination protein KB 254 1e-65 A4IND9.1 A4IND9_GEOTN Spore germination protein GerKB 251 8e-65 B4BK03.1 B4BK03_9BACI Spore germination protein 251 8e-65 >A3F903.1 A3F903_BACME GerUB Length = 366 Score = 258 bits (659), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 135/365 (36%), Positives = 231/365 (63%), Gaps = 3/365 (0%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQ 60 M+K I SQ+F ++VLFELG+A++VP+ + + WL+IL+ + +L+ +Y+ LY Sbjct: 1 MEKAKISASQLFILMVLFELGSALLVPLAIRAKQDAWLAILLGMIFSFVLFFVYHKLYTY 60 Query: 61 FPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINS 120 +PNL+ + Y +KILG +G L+ +YI M++ SR LR+ G++L++ +Y TP+ I N+ Sbjct: 61 YPNLLPTEYMQKILGKVLGTILAFIYILYFMYDASRVLRDFGEMLLTFAYPDTPLFIANA 120 Query: 121 IMVIAVMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVD 180 ++++ ++Y + KGIEV AR+ E+ F M ++ + +++ +GL++ KNL P+ + + Sbjct: 121 LLMLVIIYTIRKGIEVIARSGELLFIFMYILAVAGFILIVSSGLIEIKNLQPVLEEGLLP 180 Query: 181 ALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLG 240 +L A FPF E + FT +LP+L +S ++ + A LSG L T I +SVLG Sbjct: 181 SLKVALSETLYFPFTEAIVFTMILPYLNDSKKAKVTMLCATGLSGINLAITMLINVSVLG 240 Query: 241 LDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKV 300 +++ +R+ FP+ +T+ + VA+F++RLD +L ++IG+FFK+S+ +AA TA+LFK+ Sbjct: 241 VNLTARSQFPLLSTVESIQVADFLERLDVFFMLAMVIGIFFKVSVLFYAAVIGTANLFKI 300 Query: 301 KDYRRLVVPVGVIVLISSFISVENYTSHLNEGKLFLKYFLPV-ICAVVPILLFLVH--RI 357 K R+ P+G+I+L S N+ HL+EG K+ L + + V+P LL LV + Sbjct: 301 KSPSRMSYPLGIIILFMSMTIASNFQEHLHEGLYAAKFVLHIPLFTVIPSLLLLVSFLKN 360 Query: 358 RKRFG 362 RKR G Sbjct: 361 RKRHG 365 >A3F904.1 A3F904_BACME GerVB Length = 366 Score = 255 bits (652), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 138/366 (37%), Positives = 235/366 (64%), Gaps = 11/366 (3%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQ 60 M+K I SQ+F ++VLFELG++++VPI +++ WL+IL+ + G +L+L+Y+ L+ Sbjct: 1 MEKAKISASQLFILMVLFELGSSLLVPIAMDAKQDAWLAILIGMVGSFVLFLVYHKLHSY 60 Query: 61 FPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINS 120 +P+L+ + Y +KI+G IG L+ +YI M++ +R LR+ G +L++++Y TP+ I ++ Sbjct: 61 YPDLLPTEYMQKIMGKGIGTVLAFVYILYFMYDAARVLRDFGAMLLTSAYPETPLFIAHT 120 Query: 121 IMVIAVMYILNKGIEVFARTAEIYF---WIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKD 177 ++++ V+Y + KGIEV AR+ EI F +I +MG V +++ +GL++F NL P+ + Sbjct: 121 LLMLVVIYTIRKGIEVVARSGEILFIFIYIFAIMGFV---LIVCSGLIEFTNLQPVLEEG 177 Query: 178 WVDALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEIS 237 + L A+ FPF E + FT +LP+LK+ ++ + A LSG L T I IS Sbjct: 178 VLPVLKVAFTQTIYFPFAEAMVFTMILPYLKDQKKAKMTMLCATGLSGINLTITMLINIS 237 Query: 238 VLGLDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADL 297 VLG+D+ SR+ FP+ +T+ + VA+F++RLD +L L+IG F K+S+ +AA TA+L Sbjct: 238 VLGVDLTSRSQFPLLSTVQSIQVADFLERLDVFFMLALVIGGFIKISVLLYAAVIGTANL 297 Query: 298 FKVKDYRRLVVPVGVIVLISSFISVENYTSHLNEG---KLFLKYFLPVICAVVPILLFLV 354 FK+K RL P+G ++L + N+ HL+EG ++F+ + +P I A++P LL LV Sbjct: 298 FKIKSPSRLSYPLGFVILFMASTIASNFQEHLHEGLKVEMFILH-MP-ILAIIPSLLLLV 355 Query: 355 HRIRKR 360 ++ R Sbjct: 356 AFLKNR 361 >B7GJD3.1 B7GJD3_ANOFW Spore germination protein KB Length = 370 Score = 254 bits (648), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 136/367 (37%), Positives = 228/367 (62%), Gaps = 13/367 (3%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSIL--MALPGGVLLYLIYNDLY 58 M++ I Q+F + +LFE G+A+V+P+G+++ VWL+IL M L GG+L +Y L+ Sbjct: 1 MERACISARQLFMLAILFEHGSAMVIPLGVDAKQDVWLAILCGMVLGGGLLF--VYYRLF 58 Query: 59 RQFPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILII 118 R +P L ++ Y+++I+G++ G L L+Y+ ++ +R LR+ G+LL++ +Y TP+ I+ Sbjct: 59 RYYPRLPLTSYSQRIVGSFFGRVLGLIYVLYFLYIAARVLRDFGELLLAFAYSETPLFIL 118 Query: 119 NSIMVIAVMYILNKGIEVFARTAEIYF----WIMMVMGLVCNGVVIIAGLVDFKNLFPLH 174 N++M++ VMY + KGIEV AR ++++F WI+ ++G V ++II G VD L P+ Sbjct: 119 NAVMMLTVMYAVYKGIEVIARASQLFFFSLYWIL-ILGFV---LIIITGAVDLHRLQPIL 174 Query: 175 YKDWVDALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAI 234 + W + PFGE++ FT + P+L+ K G A+ LSG L A+ Sbjct: 175 EEGWGPIFHVVFTQTLYVPFGEMIVFTMIFPYLQKQEKVMKAGFLALFLSGINLAIIMAM 234 Query: 235 EISVLGLDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAIT 294 I+VLG DI SR+ FP+ TI + VANF++RLD L ++ LI+G FFK+ ++ +A Sbjct: 235 NIAVLGADIVSRSTFPLLNTIQEIRVANFLERLDVLFMIALIVGGFFKVGVFFYAGVIGA 294 Query: 295 ADLFKVKDYRRLVVPVGVIVLISSFISVENYTSHLNEGKLFLKYFLPV-ICAVVPILLFL 353 ADLF++K+Y+RLV P+G+ VL S N+ H+ EG + ++ + + V+P+LL L Sbjct: 295 ADLFQIKEYQRLVYPLGISVLFLSIAIASNFAEHIKEGLQIVTIYMHIPLQIVIPVLLLL 354 Query: 354 VHRIRKR 360 + +R + Sbjct: 355 IAMLRHQ 361 >A4IND9.1 A4IND9_GEOTN Spore germination protein GerKB Length = 367 Score = 251 bits (641), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 133/362 (36%), Positives = 227/362 (62%), Gaps = 1/362 (0%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQ 60 M+ I Q+F +IVLFE G+A+V+P+G+ + VW+SIL++L G+LL+ +Y L+ Sbjct: 1 MESSKINVRQLFVLIVLFEHGSAVVIPLGVSAKQDVWISILLSLAIGLLLFFVYRRLFEY 60 Query: 61 FPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINS 120 +P+ ++ Y +I+G +G L+++YI ++ +R LR+ G+LL++ +Y TP+ ++N+ Sbjct: 61 YPDQPLTAYLPQIVGAPLGKLLAIVYITYFLYIAARVLRDFGELLLTFAYPETPLFVLNA 120 Query: 121 IMVIAVMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVD 180 IM + VMY + KGIEV ART E++ ++ + ++ +V ++G+VD L P+ + W Sbjct: 121 IMAVTVMYGVYKGIEVLARTGELFLIVLYFLAIIGFVLVFVSGIVDVTQLQPMLEEGWRR 180 Query: 181 ALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLG 240 + + PFGE++ FT + P++ + GV ++LSG L AI ++VLG Sbjct: 181 VWKTVFTETLYVPFGEMIVFTMLFPYINTPTKVGRAGVVGMVLSGINLALIMAINVAVLG 240 Query: 241 LDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKV 300 D SR++FP+ TI V VA+F++RLD ++TLI G FFK+SI+ +A A+LF + Sbjct: 241 PDTVSRSSFPLLDTIRRVQVADFLERLDVFFMITLITGGFFKISIFFYAGIVGAANLFGI 300 Query: 301 KDYRRLVVPVGVIVLISSFISVENYTSHLNEGKLFLKYFLPV-ICAVVPILLFLVHRIRK 359 YRRLV P+G+++LI S + NY H+ EG + ++L V + ++P+LL ++ IR+ Sbjct: 301 SSYRRLVYPLGLLILILSVVIASNYAEHIQEGLKIVTFYLHVPLQVIIPVLLLIIAAIRR 360 Query: 360 RF 361 RF Sbjct: 361 RF 362 >B4BK03.1 B4BK03_9BACI Spore germination protein Length = 367 Score = 251 bits (641), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 133/362 (36%), Positives = 227/362 (62%), Gaps = 1/362 (0%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQ 60 M+ I Q+F +IVLFE G+A+V+P+G+ + VW+SIL++L G+LL+ +Y L+ Sbjct: 1 MESSKINVRQLFVLIVLFEHGSAVVIPLGVSAKQDVWISILLSLAIGLLLFFVYRRLFEY 60 Query: 61 FPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINS 120 +P+ ++ Y +I+G +G L+++YI ++ +R LR+ G+LL++ +Y TP+ ++N+ Sbjct: 61 YPDQPLTAYLPQIVGAPLGKLLAIVYITYFLYIAARVLRDFGELLLTFAYPETPLFVLNA 120 Query: 121 IMVIAVMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVD 180 IM + VMY + KGIEV ART E++ ++ + ++ +V ++G+VD L P+ + W Sbjct: 121 IMAVTVMYGVYKGIEVLARTGELFLIVLYFLAIIGFVLVFVSGIVDVTQLQPMLEEGWRR 180 Query: 181 ALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLG 240 + + PFGE++ FT + P++ + GV ++LSG L AI ++VLG Sbjct: 181 VWKTVFTETLYVPFGEMIVFTMLFPYINTPTKVGRAGVVGMVLSGINLALIMAINVAVLG 240 Query: 241 LDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKV 300 D SR++FP+ TI V VA+F++RLD ++TLI G FFK+SI+ +A A+LF + Sbjct: 241 PDTVSRSSFPLLDTIRRVQVADFLERLDVFFMITLITGGFFKISIFFYAGIVGAANLFGI 300 Query: 301 KDYRRLVVPVGVIVLISSFISVENYTSHLNEGKLFLKYFLPV-ICAVVPILLFLVHRIRK 359 YRRLV P+G+++LI S + NY H+ EG + ++L V + ++P+LL ++ IR+ Sbjct: 301 SSYRRLVYPLGLLILILSVVIASNYAEHIQEGLKIVTFYLHVPLQVIIPVLLLIIAAIRR 360 Query: 360 RF 361 RF Sbjct: 361 RF 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.147 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,116,613,359 Number of extensions: 87766773 Number of successful extensions: 283994 Number of sequences better than 1.0e-05: 343 Number of HSP's gapped: 283759 Number of HSP's successfully gapped: 343 Length of query: 367 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 230 Effective length of database: 1,770,017,148 Effective search space: 407103944040 Effective search space used: 407103944040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)