BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0006.p (400 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5KZH6.1 Q5KZH6_GEOKA Spore germination protein 259 4e-67 C3IZT9.1 C3IZT9_9BACI Germination protein, Ger(X)C family 255 4e-66 B7YCV1.1 B7YCV1_9BACI Germination protein, Ger(X)C family 255 4e-66 B4BK04.1 B4BK04_9BACI Germination protein, Ger(X)C family 246 4e-63 O07505.1 O07505_BACME Germination protein 244 8e-63 >Q5KZH6.1 Q5KZH6_GEOKA Spore germination protein Length = 402 Score = 259 bits (661), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 138/380 (36%), Positives = 226/380 (59%), Gaps = 5/380 (1%) Query: 20 AGCWNSRELNELAIVSGIGIDRVKETGKLRVTFQVVNPSSTT--TTQGASTNQSPISIYT 77 +GCW+ +EL +LA + IG+D+ E GK VTFQVVNP + T +G P+SIYT Sbjct: 19 SGCWSQKELTDLAFIVAIGLDKT-EDGKYLVTFQVVNPGNVAGATQRGGGAAGVPVSIYT 77 Query: 78 VNDRTVFGALRRASKQATRQLFFAHAQLLVIGESLAEGGMNEVYDIFERSHELRLNTTVL 137 T+ A R+AS++ +R ++AH L+V+GE +A G++ V D ER ++LR ++ Sbjct: 78 STGDTLVEASRKASEKLSRLPYYAHTNLIVVGEEVAREGLSGVLDAMERGNQLRPTAHLV 137 Query: 138 VSRDTDAESILRVLLPQESVPSVGLGKKAENTSRVWGETPKVSVFQTIKEI-TGGGGFTI 196 ++R A+ +L VL P + + + + K E +S+ WGET V ++ I + T G I Sbjct: 138 IARTYLAKDVLTVLTPIDKISANQIIKTIEFSSKNWGETVHVDIWGAIHCLATAGRNLVI 197 Query: 197 SGVRMLGSVEEGQKKASLEQTKAETSILISGQAVFNKGKLQNWIDGPEARGTQWILDKIV 256 +GV + G ++ G+++ +++ + + I + G A+F +L +W+DGP +RG W+LD I Sbjct: 198 TGVTVEGDLKRGKRQENVQSSVPDARIKLEGLALFKNDRLVDWVDGPSSRGVLWLLDCIR 257 Query: 257 ETNMNIDSGDQAKAIAVNVVFSKTKVKVEMRDGLPVFHIHINEEGNLNETQSVINLSDNH 316 +TN+ + + +AI VV +KT VK +++DG PV +HI EG++ ET+ I+LSD Sbjct: 258 QTNLTVSWKGKKEAINYQVVRAKTDVKAKVKDGRPVISVHIQAEGDVIETRVPIDLSDAS 317 Query: 317 EIRKLETGMEKLTEEEVREAFQAAQRAECDYFNFGNELKRLHPAEWKKVDKDW-NQIFAK 375 + +LE ++K ++EV EA + AQR + D F FG + R P WKK++ W ++ F + Sbjct: 318 MLFQLEKEIKKNLKQEVEEAIETAQRDKTDVFGFGEAVHRTDPRLWKKIESKWHDRYFPE 377 Query: 376 GTLKLQVHAYIRATGLRVTP 395 + + YIR TGLR P Sbjct: 378 LDVTVTADMYIRRTGLRNKP 397 >C3IZT9.1 C3IZT9_9BACI Germination protein, Ger(X)C family Length = 401 Score = 255 bits (652), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 131/380 (34%), Positives = 224/380 (58%), Gaps = 5/380 (1%) Query: 20 AGCWNSRELNELAIVSGIGIDRVKETGKLRVTFQVVNPSSTT--TTQGASTNQSPISIYT 77 AGCW+ +EL +LA + +G+D+ K+ GK +FQVVNP + T +G P+SIYT Sbjct: 19 AGCWSKKELTDLAFIIAVGLDKTKD-GKYLASFQVVNPGNVAGATQRGGGAAGVPVSIYT 77 Query: 78 VNDRTVFGALRRASKQATRQLFFAHAQLLVIGESLAEGGMNEVYDIFERSHELRLNTTVL 137 T+ A R+AS++ +R L++AH L+VIGE +A G++ V+D ER+ + R ++ Sbjct: 78 STGDTLVEASRKASEKMSRLLYYAHTNLMVIGEEVAREGLDGVFDALERNSQFRTTAHLV 137 Query: 138 VSRDTDAESILRVLLPQESVPSVGLGKKAENTSRVWGETPKVSVFQTIKEITGGG-GFTI 196 ++ A+ IL VL P + + + + K E + WG+T V V+Q I+++ G I Sbjct: 138 IAHGHSAKEILTVLTPVDKISANQIIKTLEFSEMAWGQTMNVDVWQVIRDLASPGRNPVI 197 Query: 197 SGVRMLGSVEEGQKKASLEQTKAETSILISGQAVFNKGKLQNWIDGPEARGTQWILDKIV 256 +GVR+ G+ G+++ +++ + + + + G A+F G+L W++GP ARG W+LD+I Sbjct: 198 TGVRIEGNPARGKRQTNVQSSVPDARVSLDGLALFKNGRLVKWVNGPTARGVVWVLDRIK 257 Query: 257 ETNMNIDSGDQAKAIAVNVVFSKTKVKVEMRDGLPVFHIHINEEGNLNETQSVINLSDNH 316 +TN+ I ++ +AI V +KT V+ ++ G P +HI EG+++E I+L D + Sbjct: 258 QTNVTIPWNEKQEAIGYRTVRAKTDVEATVKRGRPTVSVHIKAEGDISEALVPIHLDDAN 317 Query: 317 EIRKLETGMEKLTEEEVREAFQAAQRAECDYFNFGNELKRLHPAEWKKVDKDW-NQIFAK 375 + +LE +E+ ++EV++A + AQRA D F FG+ + R P WKK+ +W ++ F + Sbjct: 318 VLFQLEKEIERDIKKEVKQAIETAQRARTDVFGFGDAVHRAAPRLWKKIQNEWHDRYFPE 377 Query: 376 GTLKLQVHAYIRATGLRVTP 395 + + YIR GLR P Sbjct: 378 LDVTVTADIYIRRIGLRNKP 397 >B7YCV1.1 B7YCV1_9BACI Germination protein, Ger(X)C family Length = 401 Score = 255 bits (652), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 131/380 (34%), Positives = 224/380 (58%), Gaps = 5/380 (1%) Query: 20 AGCWNSRELNELAIVSGIGIDRVKETGKLRVTFQVVNPSSTT--TTQGASTNQSPISIYT 77 AGCW+ +EL +LA + +G+D+ K+ GK +FQVVNP + T +G P+SIYT Sbjct: 19 AGCWSKKELTDLAFIIAVGLDKTKD-GKYLASFQVVNPGNVAGATQRGGGAAGVPVSIYT 77 Query: 78 VNDRTVFGALRRASKQATRQLFFAHAQLLVIGESLAEGGMNEVYDIFERSHELRLNTTVL 137 T+ A R+AS++ +R L++AH L+VIGE +A G++ V+D ER+ + R ++ Sbjct: 78 STGDTLVEASRKASEKMSRLLYYAHTNLMVIGEEVAREGLDGVFDALERNSQFRTTAHLV 137 Query: 138 VSRDTDAESILRVLLPQESVPSVGLGKKAENTSRVWGETPKVSVFQTIKEITGGG-GFTI 196 ++ A+ IL VL P + + + + K E + WG+T V V+Q I+++ G I Sbjct: 138 IAHGHSAKEILTVLTPVDKISANQIIKTLEFSEMAWGQTMNVDVWQVIRDLASPGRNPVI 197 Query: 197 SGVRMLGSVEEGQKKASLEQTKAETSILISGQAVFNKGKLQNWIDGPEARGTQWILDKIV 256 +GVR+ G+ G+++ +++ + + + + G A+F G+L W++GP ARG W+LD+I Sbjct: 198 TGVRIEGNPARGKRQTNVQSSVPDARVSLDGLALFKNGRLVKWVNGPTARGVVWVLDRIK 257 Query: 257 ETNMNIDSGDQAKAIAVNVVFSKTKVKVEMRDGLPVFHIHINEEGNLNETQSVINLSDNH 316 +TN+ I ++ +AI V +KT V+ ++ G P +HI EG+++E I+L D + Sbjct: 258 QTNVTIPWNEKQEAIGYRTVRAKTDVEATVKRGRPTVSVHIKAEGDISEALVPIHLDDAN 317 Query: 317 EIRKLETGMEKLTEEEVREAFQAAQRAECDYFNFGNELKRLHPAEWKKVDKDW-NQIFAK 375 + +LE +E+ ++EV++A + AQRA D F FG+ + R P WKK+ +W ++ F + Sbjct: 318 VLFQLEKEIERDIKKEVKQAIETAQRARTDVFGFGDAVHRAAPRLWKKIQNEWHDRYFPE 377 Query: 376 GTLKLQVHAYIRATGLRVTP 395 + + YIR GLR P Sbjct: 378 LDVTVTADIYIRRIGLRNKP 397 >B4BK04.1 B4BK04_9BACI Germination protein, Ger(X)C family Length = 398 Score = 246 bits (627), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 127/380 (33%), Positives = 222/380 (58%), Gaps = 5/380 (1%) Query: 20 AGCWNSRELNELAIVSGIGIDRVKETGKLRVTFQVVNPSSTT--TTQGASTNQSPISIYT 77 AGCW+ REL +LA V IG+D+ +E G+ + QVVNP + +G P+SIYT Sbjct: 19 AGCWSQRELTDLAFVIAIGLDKTEE-GEYLASLQVVNPGNVAGAVPRGGGAGGVPVSIYT 77 Query: 78 VNDRTVFGALRRASKQATRQLFFAHAQLLVIGESLAEGGMNEVYDIFERSHELRLNTTVL 137 +V A R+A+++ +R L++AH L+V+ E LA G++EV+D ER H+ R ++ Sbjct: 78 STGDSVSEASRKATEKVSRLLYYAHTNLVVVSEELAREGLDEVFDAMERHHQFRTTAKLV 137 Query: 138 VSRDTDAESILRVLLPQESVPSVGLGKKAENTSRVWGETPKVSVFQTIKEI-TGGGGFTI 196 ++R A+ +L VL P + + + + + + + WGET + +++T+++I + Sbjct: 138 IARGHSAKEVLSVLTPIDKISARQIIQMLKFSELAWGETSNIDIWRTVRDIAVSQRNPVM 197 Query: 197 SGVRMLGSVEEGQKKASLEQTKAETSILISGQAVFNKGKLQNWIDGPEARGTQWILDKIV 256 +GV + G +G+++ +++ + + I + G A+F +L W+DGP ARG W+L++I Sbjct: 198 TGVAIEGDPRKGKRQENVQSSVPDARIKLDGLALFKNDRLVRWVDGPTARGMMWVLERIQ 257 Query: 257 ETNMNIDSGDQAKAIAVNVVFSKTKVKVEMRDGLPVFHIHINEEGNLNETQSVINLSDNH 316 TN+ + + +AI SKTKVK ++++G P IHI EG L+E++ INL+D Sbjct: 258 HTNLTVPWEGKKEAIGYRTTRSKTKVKAKIKNGRPSISIHIIAEGELSESRIPINLADTS 317 Query: 317 EIRKLETGMEKLTEEEVREAFQAAQRAECDYFNFGNELKRLHPAEWKKVDKDW-NQIFAK 375 + +LE +E+ +E+V +A +AAQR + D F FG + R P WKK++K+W ++ F + Sbjct: 318 LLFRLEKELERSAKEDVTKAIKAAQREKTDVFGFGAAVHRAAPRLWKKIEKEWHDRYFPE 377 Query: 376 GTLKLQVHAYIRATGLRVTP 395 + + +IR T + P Sbjct: 378 LDVTVTTDMHIRRTRMWDKP 397 >O07505.1 O07505_BACME Germination protein Length = 408 Score = 244 bits (624), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 137/376 (36%), Positives = 226/376 (60%), Gaps = 6/376 (1%) Query: 22 CWNSRELNELAIVSGIGIDRVKETGKLRVTFQVVNPSSTTTTQGASTNQSP-ISIYTVND 80 CW+ +EL +L+ VS +GID+ K+ G+ +TFQ++NP + QG QSP ++IY+ + Sbjct: 21 CWSKKELTDLSFVSAMGIDKTKD-GRYELTFQIINPGNVAGLQGGGGTQSPPVTIYSASG 79 Query: 81 RTVFGALRRASKQATRQLFFAHAQLLVIGESLA-EGGMNEVYDIFERSHELRLNTTVLVS 139 + A RRAS + +R+L++AH L+VIGE LA E G+N + D +R E R+ T+++++ Sbjct: 80 DNLVEASRRASARISRRLYYAHTNLVVIGEKLAREEGINILIDSIDRDPEFRITTSLVIA 139 Query: 140 RDTDAESILRVLLPQESVPSVGLGKKAENTSRVWGETPKVSVFQTIKEI-TGGGGFTISG 198 + A +++ L P + +P+ + K E T R WGE KVS + ++ + + GG +SG Sbjct: 140 NQSSAADLVKALTPVDKIPANKIMKTLEFTQRKWGENLKVSFQEVMRSLESPGGEALVSG 199 Query: 199 VRMLGSVEEGQKKASLEQTKAETSILISGQAVFNKGKLQNWIDGPEARGTQWILDKIVET 258 R++G+ ++ +K +L+Q+ E ++ SG AVF GKL +W+ ARG+ WILDKI T Sbjct: 200 FRLVGNPQQAEKLDNLQQSAPEATLRASGIAVFKNGKLIDWLYNSPARGSVWILDKIQGT 259 Query: 259 NMNIDSGDQAKAIAVNVVFSKTKVKVEMRDGLPVFHIHINEEGNLNETQSVINLSDNHEI 318 ++N+D ++ +A+A V KT V V+M G P +H EG+L E + ++ I Sbjct: 260 DINVDWKEKKEALAYQTVRQKTDVSVKMIKGKPYASVHTRVEGDLGEVEVPVDTRQPAVI 319 Query: 319 RKLETGMEKLTEEEVREAFQAAQRAECDYFNFGNELKRLHPAEWKKVDKDWNQ--IFAKG 376 +K+E K ++E++ A + AQ + D F FG L+R P +W+K+ +W+ F K Sbjct: 320 KKMEKAAGKEIKKELQTAIKQAQTDKVDIFGFGEALERTRPNQWEKLKSEWDDDVYFPKL 379 Query: 377 TLKLQVHAYIRATGLR 392 + + V AYIR TGLR Sbjct: 380 DVDVTVEAYIRRTGLR 395 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,152,889,454 Number of extensions: 86127332 Number of successful extensions: 183507 Number of sequences better than 1.0e-05: 404 Number of HSP's gapped: 183032 Number of HSP's successfully gapped: 416 Length of query: 400 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 262 Effective length of database: 1,760,596,133 Effective search space: 461276186846 Effective search space used: 461276186846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)