BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0005.p (521 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value O07504.1 O07504_BACME Germination protein 526 e-147 A3F901.1 A3F901_BACME GerUA 513 e-143 Q5KZH7.1 Q5KZH7_GEOKA Spore germination protein 501 e-140 B7YCV2.1 B7YCV2_9BACI GerA spore germination protein 496 e-138 A4IND7.1 A4IND7_GEOTN Spore germination protein GerKA 490 e-136 >O07504.1 O07504_BACME Germination protein Length = 540 Score = 526 bits (1354), Expect = e-147, Method: Compositional matrix adjust. Identities = 260/510 (50%), Positives = 345/510 (67%), Gaps = 10/510 (1%) Query: 6 QTKEKKQ---SQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAV 61 ++K K Q QT + T S L L ++L I++ GNS D++IRHFK+ +N +L A+ Sbjct: 9 KSKNKLQPQADQTNNEQTVSSLKNLLSENLTLIKEKTGNSSDIVIRHFKIGVNNSLAAAI 68 Query: 62 IYMEGLTDKELVDDFISHAMSFQTVSEEGQKLS-TSQEVFNYIKEKALTPGKSQSLTNRG 120 +Y+EG+ D + + D++ Q++ ++ QK Q I E +T G N Sbjct: 69 VYIEGIVDNQAIQDYL-----LQSLMKDNQKNDLNDQNALELISEDIVTMGNVSFADNWN 123 Query: 121 GLFEALLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISL 180 L +L+SG++L++V+G+ LS T GGE R ++E+ TQ+ +RG K +FTE+I N+++ Sbjct: 124 DLLSSLMSGDSLLIVDGINRVLSVSTQGGEKRAIAESTTQMVVRGPKGAFTESIGTNLAM 183 Query: 181 VRRRIKNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYI 240 VRR IK P+LW+E MK+G VT+T++ +MYIHGIA DSILESGY+ Sbjct: 184 VRRIIKTPDLWLESMKIGRVTKTDVTLMYIHGIANDKVVKEIRKRLKNIDIDSILESGYV 243 Query: 241 EQLIEGSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDY 300 EQLIE +PFPT+YNTERPD VAGNLL+GRIA+FVDGTPF LIAPA F FF + EDY Sbjct: 244 EQLIEDQTVTPFPTIYNTERPDVVAGNLLEGRIAIFVDGTPFGLIAPALFIQFFQSAEDY 303 Query: 301 YQRFDISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFV 360 Y RFDI+T S+ PA +VA FHQEM+PT L++ LA+QRE VPFPAFV Sbjct: 304 YARFDIATSIRLLRVFMFMISLIAPATYVAVTTFHQEMVPTTLIVALAAQRESVPFPAFV 363 Query: 361 EALMMEVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISS 420 EA MEVTFEI+REAGIRLP IG VSI+G LV+GQAAVQAGIVSPAMVI+VSIT I+S Sbjct: 364 EAFFMEVTFEILREAGIRLPKAIGSAVSIVGALVIGQAAVQAGIVSPAMVIIVSITAIAS 423 Query: 421 FTTPAFNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFD 480 F TP+F+MA+S RL+RF+ + +A G YGI ++ LM V HLC LRS GVPYM+PL PF Sbjct: 424 FATPSFDMAISARLIRFLFILGAATFGFYGIILILLMGVGHLCSLRSFGVPYMTPLAPFI 483 Query: 481 PQAQKDTLIRSPIHFMKIRPRLEHKSNVRK 510 P + +DTL+R P M+ RP+L + VR+ Sbjct: 484 PVSNEDTLLRLPWWTMRRRPKLISDNKVRQ 513 >A3F901.1 A3F901_BACME GerUA Length = 540 Score = 513 bits (1320), Expect = e-143, Method: Compositional matrix adjust. Identities = 253/515 (49%), Positives = 341/515 (66%), Gaps = 5/515 (0%) Query: 4 AAQTKEKKQSQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAVI 62 + + ++K A ++ + +L +L I+Q GNS D++IRH K+ N+ ++VA++ Sbjct: 10 SKKNRQKDAKNQATDQSAEHIQDSLSANLNIIKQKTGNSSDVVIRHLKMGSNSNIRVAIV 69 Query: 63 YMEGLTDKELVDDFISHAMSFQTVSEEGQKLSTSQEVFNYIKEKALTPGKSQSLTNRGGL 122 Y+EG+ D + + +F+ +M E +K++ I E + G + N L Sbjct: 70 YLEGIVDNQSIQEFLLESM---MKDNEKEKVN-EHNALELISEDMMAVGNVTFIHNWDDL 125 Query: 123 FEALLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISLVR 182 F AL+ G+TL LV+G+ +AL T GGE R ++E+ TQ+ +RG K +FTE+I N ++VR Sbjct: 126 FSALMVGDTLFLVDGIDQALGAGTQGGEKRSIAESTTQMVVRGPKGAFTESIGTNTAMVR 185 Query: 183 RRIKNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYIEQ 242 R IK P+LW E K+G VT+T++ +MYIH IA DSILESGYIEQ Sbjct: 186 RIIKTPDLWTESFKIGRVTKTDVTLMYIHDIANDKVINEIRQRLNKIDVDSILESGYIEQ 245 Query: 243 LIEGSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDYYQ 302 LIE + FPT+YNTERPD VAGNLL+GRIA+FVDGTPFVLIAPA F FF + EDYY Sbjct: 246 LIEDETMTSFPTIYNTERPDVVAGNLLEGRIAIFVDGTPFVLIAPAVFMQFFQSAEDYYA 305 Query: 303 RFDISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFVEA 362 RFDI+T S+ PA +VA FHQEM+PT L++ +A+QRE VPFPAFVEA Sbjct: 306 RFDIATSIRLLRIFMFLISLIAPATYVAVTTFHQEMVPTTLIVAIAAQREAVPFPAFVEA 365 Query: 363 LMMEVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISSFT 422 L+MEVTFEI+REAGIRLP IG VSI+G LV+GQAAVQA IVSPAMVI+VSIT I+SF Sbjct: 366 LLMEVTFEILREAGIRLPKAIGSAVSIVGSLVIGQAAVQASIVSPAMVIIVSITAIASFA 425 Query: 423 TPAFNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFDPQ 482 TP+F+MA+S RL+RF+ + +A G YGI ++ LM+V HLC LRS GVPYM+P PF P Sbjct: 426 TPSFDMAISARLIRFLFMLGAATFGFYGIILVLLMMVVHLCSLRSFGVPYMAPFAPFIPV 485 Query: 483 AQKDTLIRSPIHFMKIRPRLEHKSNVRKKADKDRQ 517 DT++R P ++ RPRL + VR+ A++ Q Sbjct: 486 NNGDTVMRLPWWTLRQRPRLISTNTVREGANQRPQ 520 >Q5KZH7.1 Q5KZH7_GEOKA Spore germination protein Length = 539 Score = 501 bits (1291), Expect = e-140, Method: Compositional matrix adjust. Identities = 256/517 (49%), Positives = 341/517 (65%), Gaps = 12/517 (2%) Query: 3 MAAQTKEKKQSQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAV 61 M + + S A PLS L ++ IRQ G S D++IR F L ++ A+ Sbjct: 14 MDVKQEGPDHSGEASDVPQEPLSAELAVNIDIIRQKTGESNDVVIRRFALGQEWQIKAAI 73 Query: 62 IYMEGLTDKELVDDFISHAM---SFQTVSEEGQKLSTSQEVFNYIKEKALTPGKSQSLTN 118 I+++GL D++ V +F+ + SF EE +E F +I++K G + +++ Sbjct: 74 IFVDGLVDEKNVYEFVLTPLLQASFPLALEE-------KERFQWIEKKLAAVGGVKHISD 126 Query: 119 RGGLFEALLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNI 178 LF L SG T++L++G+P A+S T GGE R + E TQ+++RG + FTE++ N Sbjct: 127 WRKLFFELFSGATVMLIDGVPFAISASTKGGEYRSIEEPQTQIAVRGPREGFTESLRTNT 186 Query: 179 SLVRRRIKNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESG 238 +++RRRI+NPNLW+E M++GAVTQT++AIMYI GIA D +LESG Sbjct: 187 AMIRRRIRNPNLWLETMQIGAVTQTDVAIMYIKGIANDEIVKEVRARLRRIDIDGVLESG 246 Query: 239 YIEQLIEGSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVE 298 YIEQLIE ++ FPT Y+TERPD VA NLL+GR+AVFV+GTPFVL+APA F FF VE Sbjct: 247 YIEQLIEDQTFTTFPTTYHTERPDIVAANLLEGRVAVFVEGTPFVLVAPALFIQFFQAVE 306 Query: 299 DYYQRFDISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPA 358 DYY RFDIST S+ PAI++AA FHQEMIPT L+I +A+QRE VPFP Sbjct: 307 DYYARFDISTALRFLRVLIFFISLVAPAIYIAATTFHQEMIPTQLVIAIAAQREAVPFPG 366 Query: 359 FVEALMMEVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGI 418 FVEAL+MEVTFEI+REAG+RLP +GQ VSI+G LV+GQAAV+AG VS AMVIVVSIT I Sbjct: 367 FVEALVMEVTFEILREAGVRLPRAVGQAVSIVGALVIGQAAVEAGFVSSAMVIVVSITAI 426 Query: 419 SSFTTPAFNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGP 478 +SF TP+F +A+S RL+RF ++F++A G YGI + L++ HLC LRS GVPYMSPL P Sbjct: 427 ASFATPSFAIAISARLIRFGLMFLAAMFGFYGIMMGILVMTIHLCSLRSFGVPYMSPLAP 486 Query: 479 FDPQAQKDTLIRSPIHFMKIRPRLEHKSN-VRKKADK 514 F P DTL R P + RPRL ++ N VR+ D+ Sbjct: 487 FIPTNMGDTLFRLPTWMFRERPRLINQKNIVRQSGDQ 523 >B7YCV2.1 B7YCV2_9BACI GerA spore germination protein Length = 541 Score = 496 bits (1276), Expect = e-138, Method: Compositional matrix adjust. Identities = 252/516 (48%), Positives = 340/516 (65%), Gaps = 12/516 (2%) Query: 4 AAQTKEKKQSQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAVI 62 + Q + S A PLS L ++ IRQ G S D++IR F L ++ A+I Sbjct: 17 SVQQEGPDHSGEASDVPQEPLSAELAVNIDIIRQKTGESNDIVIRRFALGQEWQIKAAII 76 Query: 63 YMEGLTDKELVDDFISHAM---SFQTVSEEGQKLSTSQEVFNYIKEKALTPGKSQSLTNR 119 +++GL D++ V +F+ + SF EE +E F +I++K G + +++ Sbjct: 77 FVDGLVDEKNVYEFVLTPLLQASFPLALEE-------KERFQWIEKKLAAVGGVKHISDW 129 Query: 120 GGLFEALLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNIS 179 G LF L SG T++L++G+ A+S T GG+ R V E TQ+++RG + FTE++ N + Sbjct: 130 GKLFFELFSGATVILIDGVSFAVSASTKGGQYRAVEEPQTQIAVRGPREGFTESLRTNTA 189 Query: 180 LVRRRIKNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGY 239 ++RRRI+NPNLW+E M++G VTQT++AIMYI GI D +LESGY Sbjct: 190 MIRRRIRNPNLWLETMQIGTVTQTDVAIMYIKGIVNDGIVKEVRERLRRIDIDGVLESGY 249 Query: 240 IEQLIEGSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVED 299 IEQLIE ++ FPT Y+TERPD VA NLL+GR+AVFV+GTPFVL+APA F FF VED Sbjct: 250 IEQLIEDQTFTTFPTTYHTERPDIVAANLLEGRVAVFVEGTPFVLLAPAVFIQFFQAVED 309 Query: 300 YYQRFDISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAF 359 YY RFDI+T S+ PAI++A+ FHQEMIPT L+I +A+QRE VPFP F Sbjct: 310 YYARFDIATALRFLRVLIFFISLVAPAIYIASTTFHQEMIPTQLVIAIAAQREAVPFPGF 369 Query: 360 VEALMMEVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGIS 419 VEAL+MEVTFEI+REAG+RLP +GQ VSI+G LV+GQAAV+AG VS AMVIVVSIT I+ Sbjct: 370 VEALVMEVTFEILREAGVRLPRAVGQAVSIVGALVIGQAAVEAGFVSSAMVIVVSITAIA 429 Query: 420 SFTTPAFNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPF 479 SF TP+F +A+S RL+RF ++F++A G YGI + L+++ HLC LRS GVPYMSPL PF Sbjct: 430 SFATPSFAIAISARLIRFGLMFLAAMFGFYGIMMGILVMMIHLCSLRSFGVPYMSPLAPF 489 Query: 480 DPQAQKDTLIRSPIHFMKIRPRLEHKSN-VRKKADK 514 P DTL R P + RPRL ++ N VR+ D+ Sbjct: 490 IPTNMGDTLFRLPTWMFRERPRLINQKNIVRQSGDQ 525 >A4IND7.1 A4IND7_GEOTN Spore germination protein GerKA Length = 541 Score = 490 bits (1261), Expect = e-136, Method: Compositional matrix adjust. Identities = 251/534 (47%), Positives = 342/534 (64%), Gaps = 24/534 (4%) Query: 1 LLMAAQTKEKKQ-----SQTADSNT-------SSPLSGNLKDDLQHIRQGLGNSMDLIIR 48 L ++KKQ Q D ++ P+S L ++ IR+ G S D++IR Sbjct: 2 LFQWGMRRQKKQMNDGVQQEGDDHSGEVADVPQEPMSAELAVNIDIIRRATGESDDVVIR 61 Query: 49 HFKL-INAALQVAVIYMEGLTDKELVDDFISHAM---SFQTVSEEGQKLSTSQEVFNYIK 104 F L ++ AV++++GL D++ V +F+ + SF EE ++L +I+ Sbjct: 62 RFSLGQEKQMKAAVVFVDGLVDEKNVYEFLLTPLLTASFPLALEEKERL-------QWIE 114 Query: 105 EKALTPGKSQSLTNRGGLFEALLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIR 164 +K G + + + LF L SG T+++++G A+ T GG+ R + E TQ+++R Sbjct: 115 QKLAAVGGVKHVADWKLLFCELFSGATVMMIDGTSFAICASTKGGQYRSIEEPQTQIAVR 174 Query: 165 GSKASFTETISVNISLVRRRIKNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXX 224 G + FTE++ N +++RRRIKNPNLW+E M++G VTQT++AIMY+ GIA Sbjct: 175 GPREGFTESLRTNTAMIRRRIKNPNLWLETMQIGTVTQTDVAIMYVKGIANDEIIEEVKK 234 Query: 225 XXXXXXXDSILESGYIEQLIEGSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVL 284 D +LESGYIEQLIE ++PFPT Y+TERPD VA NLL+GR+A+FV+GTPFVL Sbjct: 235 RLRRIDIDGVLESGYIEQLIEDQTFTPFPTTYHTERPDIVAANLLEGRVAIFVEGTPFVL 294 Query: 285 IAPATFFMFFHTVEDYYQRFDISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLL 344 +APA F FF VEDYY RFDI+T S+ PA ++A FHQEMIPT L+ Sbjct: 295 VAPALFIQFFQAVEDYYARFDIATALRFLRVLIFFISLVAPAAYIAGTMFHQEMIPTLLV 354 Query: 345 INLASQREGVPFPAFVEALMMEVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGI 404 I +A+QRE VPFPAFVEAL+MEVTFEI+REAGIRLP +GQ VSI+G LV+GQAAV+AG+ Sbjct: 355 IAIAAQREAVPFPAFVEALIMEVTFEILREAGIRLPRAVGQAVSIVGALVIGQAAVEAGL 414 Query: 405 VSPAMVIVVSITGISSFTTPAFNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCG 464 VS AMVIVVSIT I+SF TP+F +A+S RL+RF ++F++A G YGI + L++ HLC Sbjct: 415 VSSAMVIVVSITAIASFATPSFAIAISARLIRFALMFLAAMFGFYGIMMGLLVMTIHLCS 474 Query: 465 LRSLGVPYMSPLGPFDPQAQKDTLIRSPIHFMKIRPRLEHKSN-VRKKADKDRQ 517 LRS GVPYMSPL PF P DTL R P + RPRL + N VR+ D+ Q Sbjct: 475 LRSFGVPYMSPLAPFMPSNMGDTLFRLPTWMFRERPRLISQKNIVRQSGDQKPQ 528 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,731,089,526 Number of extensions: 105465763 Number of successful extensions: 305078 Number of sequences better than 1.0e-05: 1095 Number of HSP's gapped: 302587 Number of HSP's successfully gapped: 1096 Length of query: 521 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 381 Effective length of database: 1,741,754,103 Effective search space: 663608313243 Effective search space used: 663608313243 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)