BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0053.0005.p (488 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2BF74.1 Q2BF74_9BACI Sucrose phosphorylase 778 0.0 C2LT72.1 C2LT72_STRSL Sucrose phosphorylase EC=2.4.1.7 684 0.0 A8ZUP8.1 A8ZUP8_DESOH Sucrose phosphorylase EC=2.4.1.7 450 e-124 A8VXG7.1 A8VXG7_9BACI Sucrose phosphorylase EC=2.4.1.7 408 e-112 B9WVU8.1 B9WVU8_STRSU Sucrose phosphorylase EC=2.4.1.7 400 e-109 >Q2BF74.1 Q2BF74_9BACI Sucrose phosphorylase Length = 489 Score = 778 bits (2008), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/487 (76%), Positives = 418/487 (85%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 VKNQVQLITYPDSLGGDLK L+ + +F D+FKGGVHILPPFPSSGDRGFAPLTYLEI+ Sbjct: 3 VKNQVQLITYPDSLGGDLKMLNKVMTEHFADIFKGGVHILPPFPSSGDRGFAPLTYLEID 62 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 P FG W DIR IGE D+LVDLMVNHIS++S YFQD L GRQS+YADLF+TLDK+W DG Sbjct: 63 PEFGGWEDIRKIGENFDILVDLMVNHISQKSEYFQDFLAKGRQSQYADLFITLDKLWEDG 122 Query: 122 QPVQADIDKMFLRRSKPYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFIQ 181 QPVQ DIDKMFLRR PYSTFTI++TGE+ VWTTFGK DPSEQIDLDI+SE+V+QL Sbjct: 123 QPVQEDIDKMFLRRPLPYSTFTIEETGEQETVWTTFGKTDPSEQIDLDIKSEQVRQLLTD 182 Query: 182 FFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVHS 241 FFT FKE N+KIVRLDAVGYV+KKLGTSCFFVEPEIY FLDWI ELA+SL IELLPEVHS Sbjct: 183 FFTNFKEQNVKIVRLDAVGYVVKKLGTSCFFVEPEIYEFLDWIMELANSLGIELLPEVHS 242 Query: 242 HYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPVK 301 HYSIQ+KLAEHG WIYDFILPYR+L+TL+NK S+ L YL+ RP KQFTMLDCHDG+PVK Sbjct: 243 HYSIQHKLAEHGFWIYDFILPYRVLETLINKSSKNLAIYLKNRPHKQFTMLDCHDGIPVK 302 Query: 302 PDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDAY 361 PDLD LIDTK+A+ IVD+C+ERG+NLSLILSDEHKA DGFDVHQIRCSYYSVLN DDDAY Sbjct: 303 PDLDGLIDTKEAKDIVDVCLERGSNLSLILSDEHKAEDGFDVHQIRCSYYSVLNSDDDAY 362 Query: 362 LAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVVQ 421 LAARAIQFF PG+PQVYYVGLLAGEND+E V TGEGREINR N+TL EI +SLEK VVQ Sbjct: 363 LAARAIQFFTPGVPQVYYVGLLAGENDVENVARTGEGREINRHNFTLEEIEESLEKPVVQ 422 Query: 422 RLLKLIRFRNEYEAFNGDFTVEEAAADEIRLSWKKGEFRCTLYINLDTMRSEIEYTDGEG 481 RLLKLIRFRNE EAF+G+F V ++ EIRLSW+ G C+L+I+L+T RS IEY G Sbjct: 423 RLLKLIRFRNENEAFSGEFQVLDSPDSEIRLSWRNGSKHCSLFIDLETNRSTIEYVGESG 482 Query: 482 VLAQLEV 488 +AQ + Sbjct: 483 EMAQYNI 489 >C2LT72.1 C2LT72_STRSL Sucrose phosphorylase EC=2.4.1.7 Length = 487 Score = 684 bits (1764), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/481 (66%), Positives = 390/481 (81%), Gaps = 1/481 (0%) Query: 1 MVKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEI 60 MVKN+VQLITYPDSLGG+LK+L LD YFP++F+GG+HILPP+PSSGDRGFAPLTY EI Sbjct: 1 MVKNKVQLITYPDSLGGNLKALKSNLDTYFPNLFEGGIHILPPYPSSGDRGFAPLTYFEI 60 Query: 61 EPAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWAD 120 +P FGDW D+R + E +D+L+D+MVNHIS++SPYFQD L++GR S+YAD FLTL+KIW D Sbjct: 61 DPQFGDWEDVRNLAEDYDILLDIMVNHISQKSPYFQDFLKNGRDSQYADYFLTLEKIWED 120 Query: 121 GQPVQADIDKMFLRRSKPYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 G PVQ+DID+MFLRR PYS F I+ TGE +VWTTFGK PSEQIDLD+ SE+VKQLF+ Sbjct: 121 GVPVQSDIDQMFLRRKVPYSEFIIEKTGEVEKVWTTFGKTTPSEQIDLDVHSEQVKQLFV 180 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVH 240 F F +N +KI+RLDAVGYV+KKLGTSCFFVEP+IY FLDWI +A S DI LLPEVH Sbjct: 181 DIFKHFHDNGVKIIRLDAVGYVLKKLGTSCFFVEPDIYDFLDWILGVAKSYDIALLPEVH 240 Query: 241 SHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV 300 +HYSIQYKLAEHGCWIYDFILPYRI + L N++S+ L +YLRTRP QFTMLDCHDG+PV Sbjct: 241 AHYSIQYKLAEHGCWIYDFILPYRIFEALSNEESKFLVDYLRTRPHGQFTMLDCHDGLPV 300 Query: 301 KPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDA 360 KPDLD LID+KDA+K+VD+ ++RGANLSLI+SDEHK DGFDVHQIR + YS+ + DDD Sbjct: 301 KPDLDGLIDSKDARKLVDLALDRGANLSLIVSDEHKDEDGFDVHQIRSTIYSLFDEDDDT 360 Query: 361 YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVV 420 YLAARA+Q F PGIPQ+YYVGLLAG+ND+E V G+GREINR N+ EI + E EVV Sbjct: 361 YLAARALQVFTPGIPQIYYVGLLAGKNDIEAVEARGDGREINRHNFDEKEIAAAAETEVV 420 Query: 421 QRLLKLIRFRNEYEAFNGDFTVEEAAADEIRLSWKKGEFRCTLYINLDTMRSEIEYT-DG 479 QRL+ LIRFRN++ AF GDFT + + E+ LSWK +L I+L + YT DG Sbjct: 421 QRLVNLIRFRNQHPAFEGDFTAQAPSPTELVLSWKNDAECASLTIDLKAKTGHVAYTEDG 480 Query: 480 E 480 + Sbjct: 481 Q 481 >A8ZUP8.1 A8ZUP8_DESOH Sucrose phosphorylase EC=2.4.1.7 Length = 482 Score = 450 bits (1158), Expect = e-124, Method: Compositional matrix adjust. Identities = 217/479 (45%), Positives = 314/479 (65%), Gaps = 8/479 (1%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 ++N++Q+I+YPDSLG +L+ LH+ L YF D GGVH+LP +PSS DRGFAP+TY +++ Sbjct: 3 LRNRIQVISYPDSLGKNLQELHYVLRRYFADA-AGGVHLLPFYPSSADRGFAPVTYYQVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 PAFG W DI I E D+ +D MVNHISRQS Y D ++ G +S YAD+FL+ +K+ DG Sbjct: 62 PAFGTWRDIELIQEDFDITIDFMVNHISRQSKYVADFIEKGDKSEYADMFLSFNKLAPDG 121 Query: 122 QPVQADIDKMFLRRSKPYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFIQ 181 + + D+DK++ R+ P + G +W+TF EQID+D++S ++LF Sbjct: 122 EIRKEDLDKVYTRKPSPPYQVIERPDGSRETIWSTFDY----EQIDVDVQSPVTRELFRD 177 Query: 182 FFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVHS 241 F + +R+DA Y +LGT+CFFVEPE++ L+ ++ ++LLPEVH Sbjct: 178 FLIFLSRRKPEFIRMDAFAYTTIRLGTNCFFVEPEVWELLEMLEGFVSPFGVQLLPEVHE 237 Query: 242 HYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPVK 301 HYS Q KLAE G W+YDF LP +L TL + ++ L +LR P +QFT LD HDG+ V Sbjct: 238 HYSYQLKLAEKGYWVYDFALPMLVLHTLYHHTNKRLLHWLRICPRRQFTTLDTHDGLGVV 297 Query: 302 PDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDAY 361 D+ DL+ ++ ++ VD E+G+N+ I S D++Q+ C+YYS L +DD+Y Sbjct: 298 -DVADLMSQEEIERTVDGLYEKGSNIKRIYSTADY--QNLDIYQVNCTYYSALERNDDSY 354 Query: 362 LAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVVQ 421 LAAR +QF+APGIP VYYVGLLAGEND+ V T GR+INR NYTL EI ++E+EVVQ Sbjct: 355 LAARTLQFYAPGIPHVYYVGLLAGENDVALVEATRNGRDINRHNYTLDEIAVAVEREVVQ 414 Query: 422 RLLKLIRFRNEYEAFNGDFTVEEAAADEIRLSWKKGEFRCTLYINLDTMRSEIEYTDGE 480 RL++L+RFR+ Y AF+G+ +EE + + L+W + T ++LD+ R+EI + D + Sbjct: 415 RLIRLMRFRSSYPAFDGELVIEETPENRVALTWNMPPYTTTARVDLDSYRTEITFWDAD 473 >A8VXG7.1 A8VXG7_9BACI Sucrose phosphorylase EC=2.4.1.7 Length = 483 Score = 408 bits (1049), Expect = e-112, Method: Compositional matrix adjust. Identities = 208/479 (43%), Positives = 297/479 (62%), Gaps = 11/479 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 VKN++ LITY DSLG +LK L L+ +F D GGVH+LP +PSS DRGFAP+TY E++ Sbjct: 3 VKNEIMLITYADSLGKNLKELDEVLETHFKDAV-GGVHLLPFYPSSADRGFAPMTYQEVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 PAFG W+D+ I +++D + D M+NHIS+ S YFQD ++ S YADLF+ W G Sbjct: 62 PAFGGWSDVERIAKKYDTMYDFMINHISKSSEYFQDFMEKKDDSPYADLFIQYKDFWPGG 121 Query: 122 QPVQADIDKMFLRRSK-PYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 +P Q D+DK++ R+ + PY T D E ++W TF + EQIDL++ E+ K Sbjct: 122 EPTQEDVDKIYKRKPRAPYIDVTFGDGSTE-KIWCTFDE----EQIDLNVYHERTKAFMR 176 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVH 240 + T E I+RLDA Y KK GT CFF+EP+ + LD I+ + + + E+LPE+H Sbjct: 177 ENLTFLAEKGASIIRLDAFAYATKKPGTDCFFLEPDTWEMLDEIQTILEPIGCEILPEIH 236 Query: 241 SHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV 300 HY+IQ KLAE G ++YDF LP +L L + + L +L P KQ+T LD HDG+ V Sbjct: 237 EHYTIQLKLAERGYYVYDFALPMLVLHALYSGRTDRLAHWLNIAPRKQYTTLDTHDGIGV 296 Query: 301 KPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDA 360 D+ DL+ ++ + D +GAN+ + + + D++Q+ C+YYS L DD A Sbjct: 297 -VDVADLMTQEEIEATRDDLFTKGANVKRVYNTMEY--NNLDIYQLNCTYYSALGDDDQA 353 Query: 361 YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVV 420 YL ARAIQFFAPGIPQVYYVGLLAG+ND+E + ET GR INR YT EI + +++ V+ Sbjct: 354 YLLARAIQFFAPGIPQVYYVGLLAGKNDIELLEETKVGRNINRHYYTKEEIAEEMKRPVM 413 Query: 421 QRLLKLIRFRNEYEAFNGDFTVEEAAADEI-RLSWKKGEFRCTLYINLDTMRSEIEYTD 478 + +KL+ FRN+Y+AF+ + E ++ + G+ + L N++T I Y D Sbjct: 414 AKFMKLMTFRNDYKAFDDQIDITEREDGQVLEIVRTTGDNQAILTANVETHAFTITYKD 472 >B9WVU8.1 B9WVU8_STRSU Sucrose phosphorylase EC=2.4.1.7 Length = 482 Score = 400 bits (1029), Expect = e-109, Method: Compositional matrix adjust. Identities = 208/456 (45%), Positives = 292/456 (64%), Gaps = 10/456 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 +KNQ LITY DSLG +LK L L+ DV GG+HILP FPSSGDRGFAP+ Y +++ Sbjct: 3 IKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVV-GGIHILPFFPSSGDRGFAPMDYTKVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 PAFGDW+DI A+ + + ++ D M+NHIS +SPYF D L+ +S YADLF+ W +G Sbjct: 62 PAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKKDESDYADLFIRYKNFWPNG 121 Query: 122 QPVQADIDKMFLRRSK-PYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 +P Q D+D ++ R+ + PY T D EE +VW TF + +QIDLD+ +E ++ Sbjct: 122 EPTQEDVDLIYKRKPRAPYRIATFADGSEE-KVWCTFDE----QQIDLDVTTETTRRFIQ 176 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVH 240 + E+ I+RLDA Y KKLGT+CFFVEP+I+ L + ++L D+E+LPE+H Sbjct: 177 ENLEQLAEHGASIIRLDAFAYANKKLGTNCFFVEPDIWEMLHFPRQLLAPKDVEILPEIH 236 Query: 241 SHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV 300 HY+IQ K+AE ++YDF LP +L L + L ++ P KQFT LD HDG+ V Sbjct: 237 EHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHWMEICPRKQFTTLDTHDGIGV 296 Query: 301 KPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDA 360 D+ DL+ ++ ++ + +GAN+ I S E A + D++QI C+YYS L +D A Sbjct: 297 V-DVKDLLTDEETEETREALYAQGANVKKIYSTE--AYNNLDIYQINCTYYSALGNNDQA 353 Query: 361 YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVV 420 YL AR +Q FAPGIPQ+YYVGLLAGEND+E + + EGR INR Y L EI Q +++ VV Sbjct: 354 YLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGRNINRHYYDLEEIEQEVQRPVV 413 Query: 421 QRLLKLIRFRNEYEAFNGDFTVEEAAADEIRLSWKK 456 Q L KL++FRN AF+G+F+V+ + + W Sbjct: 414 QSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWNN 449 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.141 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,143,138,977 Number of extensions: 139977055 Number of successful extensions: 288442 Number of sequences better than 1.0e-05: 535 Number of HSP's gapped: 287847 Number of HSP's successfully gapped: 537 Length of query: 488 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 348 Effective length of database: 1,741,754,103 Effective search space: 606130427844 Effective search space used: 606130427844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)