BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0002.p (318 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DDB6.1 B1DDB6_9BACL Metal dependent phosphohydrolase 508 e-142 B3KC69.1 B3KC69_9BACI Metal-dependent phosphohydrolase HD sub do... 498 e-139 C5D6L2.1 C5D6L2_9BACI Metal dependent phosphohydrolase 234 1e-59 Q5WHU5.1 YHAM_BACSK 3'-5' exoribonuclease yhaM EC=3.1.-.- 228 7e-58 Q97GV4.1 Q97GV4_CLOAB HD-hydrolase domain containing protein, YH... 227 1e-57 >B1DDB6.1 B1DDB6_9BACL Metal dependent phosphohydrolase Length = 318 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 237/318 (74%), Positives = 271/318 (85%) Query: 1 MTLIKQLSPQDEFVGFYLLRELAIKQTNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKE 60 MT IK L QD+F GFYLL+EL +KQTN T PKDYFDIVL D +GQ+ AK+WD S+ DKE Sbjct: 1 MTQIKDLRNQDDFTGFYLLKELEVKQTNATTPKDYFDIVLADGTGQLGAKFWDVSSQDKE 60 Query: 61 TFFPMALVKIRGIAHTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGV 120 TFFPM LVK++G H YREKLQ+KI+++R A + D ++LTDFIRSAP+ +LI TI Sbjct: 61 TFFPMGLVKVQGNVHLYREKLQIKISRMRPAVEEDGITLTDFIRSAPVASNELIDTIYKA 120 Query: 121 MASITDPEIATIVSFCVGKVEKKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRP 180 SI D EI IV++CV KVE+KL HYPAAKTHHHAYFAGLAYHM RMLEIGEFLCKQRP Sbjct: 121 KDSIQDTEIKAIVTYCVTKVEEKLTHYPAAKTHHHAYFAGLAYHMARMLEIGEFLCKQRP 180 Query: 181 FLNPDLMKAGIILHDIAKPEEMISQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDL 240 FLN DL+KAGIILHDIAKPEEMI+Q GIVSDYS+ GKL+GHIS+AS WITEAAIR I+L Sbjct: 181 FLNADLLKAGIILHDIAKPEEMIAQLGIVSDYSLSGKLMGHISLASTWITEAAIRCDINL 240 Query: 241 ESEKVLGLQHLVLSHHNLGEWGSPVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEE 300 +SEKVLGLQH+VLSHHNLGEWGSPVQPQTAEAVALHHIDA+DAKLQMVEDAL TTPE+E Sbjct: 241 DSEKVLGLQHMVLSHHNLGEWGSPVQPQTAEAVALHHIDAMDAKLQMVEDALQTTPESEP 300 Query: 301 WTPFIRGLENKAVYRMKI 318 WTP +RGLENK +YRMK+ Sbjct: 301 WTPVVRGLENKPIYRMKL 318 >B3KC69.1 B3KC69_9BACI Metal-dependent phosphohydrolase HD sub domain Length = 295 Score = 498 bits (1282), Expect = e-139, Method: Compositional matrix adjust. Identities = 230/295 (77%), Positives = 264/295 (89%) Query: 24 IKQTNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKETFFPMALVKIRGIAHTYREKLQV 83 ++QTNGTPPKDYFDIVL D+SGQISAKYWD + DKETF PM LVKI+G +YR++ QV Sbjct: 1 MRQTNGTPPKDYFDIVLADASGQISAKYWDVTIQDKETFLPMQLVKIQGTVLSYRDQPQV 60 Query: 84 KITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGVMASITDPEIATIVSFCVGKVEKK 143 KI++IR A D D VSLTDF+R+APIRPVDLIHTIK M IT+P++ +IV +CV KVE+K Sbjct: 61 KISRIRKATDADGVSLTDFVRAAPIRPVDLIHTIKLTMNEITEPQVRSIVQYCVSKVEEK 120 Query: 144 LMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRPFLNPDLMKAGIILHDIAKPEEMI 203 LMHYPAAKT+HHAY+AGLAYH+VRMLEIG+F+CKQRPFLNPDL++AGIILHDIAKPEEMI Sbjct: 121 LMHYPAAKTYHHAYYAGLAYHIVRMLEIGDFICKQRPFLNPDLIRAGIILHDIAKPEEMI 180 Query: 204 SQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDLESEKVLGLQHLVLSHHNLGEWGS 263 +Q GIV+DYS QGKLIGHI++ASNWI EAAI +GI LESE VLGLQHLVLSHHNLGEWGS Sbjct: 181 AQMGIVTDYSNQGKLIGHIALASNWIVEAAIENGIALESEVVLGLQHLVLSHHNLGEWGS 240 Query: 264 PVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEEWTPFIRGLENKAVYRMKI 318 PVQPQTAEAVALHHID LDAKLQMVEDALDTT ETE WTP IRGL+NKA+YR+K+ Sbjct: 241 PVQPQTAEAVALHHIDLLDAKLQMVEDALDTTIETEPWTPMIRGLDNKAIYRLKV 295 >C5D6L2.1 C5D6L2_9BACI Metal dependent phosphohydrolase Length = 324 Score = 234 bits (596), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 125/306 (40%), Positives = 188/306 (61%), Gaps = 10/306 (3%) Query: 12 EFVGFYLLRELAIK--QTNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKETFFPMALVK 69 E V YLL + K +NG P + ++L D +G I AK WD S D+ + P ++VK Sbjct: 12 EQVDVYLLIKSVTKGIASNGKP---FLTLILQDKTGDIEAKLWDVSPEDESVYVPESIVK 68 Query: 70 IRGIAHTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGVMASITDPEI 129 + G H YR K+Q+KI IRLA++GD V ++DF+ +AP++ D++ I + ++ +P I Sbjct: 69 VLGDIHNYRGKMQLKIRSIRLAHEGDAVRVSDFLETAPMKREDMMEKITEYIFAMQNPNI 128 Query: 130 ATIVSFCVGKVEKKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRPFLNPDLMKA 189 I + + K E+K YPAA +HH + +GLAYH+V MLE+ + L P LN DL+ A Sbjct: 129 QRITRYLLKKYEQKFFDYPAATKNHHEFISGLAYHVVSMLELAKALVNLYPSLNRDLLYA 188 Query: 190 GIILHDIAKPEEMISQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDLESEKVLGLQ 249 G+ILHD+ K E+ + + Y+++GKL+GHI + I++AA GI E+++ LQ Sbjct: 189 GVILHDLGKVIELSGP--VSASYTLEGKLLGHIPIMVGEISKAAEHLGIS--GEEIVVLQ 244 Query: 250 HLVLSHHNLGEWGSPVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEEWTPFIRGLE 309 H+VLSHH EWGSP P EA LH+ID LDAK+ M++ AL+ + E+T I LE Sbjct: 245 HMVLSHHGKAEWGSPKPPMVKEAEILHYIDNLDAKMNMIDRALEKV-KPGEFTERIYALE 303 Query: 310 NKAVYR 315 N++ Y+ Sbjct: 304 NRSFYK 309 >Q5WHU5.1 YHAM_BACSK 3'-5' exoribonuclease yhaM EC=3.1.-.- Length = 319 Score = 228 bits (581), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 125/304 (41%), Positives = 186/304 (61%), Gaps = 6/304 (1%) Query: 12 EFVGFYLLRELAIKQTNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKETFFPMALVKIR 71 E V YLL + A KQ + K + ++LGD +G+I AK W S D+ TF +V I+ Sbjct: 12 EAVDGYLLIKTATKQI-ASNSKPFLTLILGDHTGEIEAKLWGISPEDEATFVNKTIVHIQ 70 Query: 72 GIAHTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGVMASITDPEIAT 131 G YR +LQ+KI IR + D V L DF+RSAP+ P D++ + + I +P I Sbjct: 71 GDVIDYRGRLQLKIASIRPTSAMDGVHLNDFVRSAPLAPNDMLDEVNQYIFEIHNPHIQR 130 Query: 132 IVSFCVGKVEKKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRPFLNPDLMKAGI 191 I F + K ++ + PAA +HH + +GLA+H+V ML IG+ L + P L+ DL+ AGI Sbjct: 131 ITRFLLKKHQQAFLESPAATKNHHEFMSGLAFHVVSMLRIGKSLIELYPTLDKDLLYAGI 190 Query: 192 ILHDIAKPEEMISQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDLESEKVLGLQHL 251 ILHD+ K E+ I + Y+++GKL+GHIS+ SN I +AA ++++ E+VL LQHL Sbjct: 191 ILHDLGKVRELSG--AIDTTYTLEGKLLGHISIMSNEIAQAA--KELEIDGEEVLILQHL 246 Query: 252 VLSHHNLGEWGSPVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEEWTPFIRGLENK 311 +LSHH GEWGSP P EA LH ID +DAK+ M++ AL+ + E++ + L+N+ Sbjct: 247 ILSHHGKGEWGSPKTPVVREAEVLHLIDNIDAKINMMDRALERV-QPGEFSERVMALDNR 305 Query: 312 AVYR 315 + Y+ Sbjct: 306 SFYK 309 >Q97GV4.1 Q97GV4_CLOAB HD-hydrolase domain containing protein, YHAM B.subtilis ortholog Length = 322 Score = 227 bits (579), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 123/315 (39%), Positives = 190/315 (60%), Gaps = 6/315 (1%) Query: 4 IKQLSPQDEFVGFYLLRELAIKQTNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKETFF 63 I +L + GF++++ + ++ + K Y D GD +G I+ K WDAS D++ F Sbjct: 6 ISELEAGNRIDGFFIIK--SAEKRISSNNKKYLDFTFGDKTGDINGKLWDASDEDEDAFV 63 Query: 64 PMALVKIRGIAHTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGVMAS 123 LVK+RG ++ LQ+KI KIR D DNV + DF+ SAP P D+ TI + Sbjct: 64 DNMLVKVRGTVIEWQNNLQLKIDKIRKTVDSDNVKVADFVPSAPYLPDDMYSTILEYVEK 123 Query: 124 ITDPEIATIVSFCVGKVEKKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRPFLN 183 I + +I I+ + +++M++PAAK +HH+ +GL YH ML++GE + + FLN Sbjct: 124 IKNADIKNILYDVLESAGEEIMYWPAAKKNHHSVRSGLLYHTFTMLKVGEKISEIYTFLN 183 Query: 184 PDLMKAGIILHDIAKPEEMI-SQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDLES 242 DL+ AG+ILHD+AK EEM+ S+ GIV+DYSV+G+L+GHI + + +A + G D Sbjct: 184 TDLIYAGVILHDMAKLEEMVSSELGIVTDYSVEGQLLGHIILGVEKVRNSAKKVGAD--K 241 Query: 243 EKVLGLQHLVLSHHNLGEWGSPVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEEWT 302 E + L+H+VLSHH E+GSP +P EA LHH+D +DA + ++ AL+TT E + Sbjct: 242 EISMMLEHMVLSHHYEAEYGSPKKPMFPEAEVLHHLDDMDAAMFDMKKALETTAPG-EMS 300 Query: 303 PFIRGLENKAVYRMK 317 I L+ + +Y+ K Sbjct: 301 DAIWSLDRRRIYKSK 315 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,882,280,083 Number of extensions: 75166004 Number of successful extensions: 166725 Number of sequences better than 1.0e-05: 428 Number of HSP's gapped: 165899 Number of HSP's successfully gapped: 428 Length of query: 318 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 183 Effective length of database: 1,788,859,178 Effective search space: 327361229574 Effective search space used: 327361229574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)