BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0009.p (339 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K859.1 B3K859_9BACI Radical SAM enzyme, Cfr family 541 e-152 Q5WKB6.1 RLMN1_BACSK Ribosomal RNA large subunit methyltransfera... 537 e-151 B1DCW9.1 B1DCW9_9BACL Radical SAM enzyme, Cfr family 532 e-149 B1YG36.1 RLMN_EXIS2 Ribosomal RNA large subunit methyltransferas... 513 e-143 C1PD26.1 C1PD26_BACCO Radical SAM enzyme, Cfr family 510 e-143 >B3K859.1 B3K859_9BACI Radical SAM enzyme, Cfr family Length = 353 Score = 541 bits (1395), Expect = e-152, Method: Compositional matrix adjust. Identities = 253/339 (74%), Positives = 297/339 (87%) Query: 1 MDKQSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEH 60 M+K SIYGLTL+Q+ +WL E GHKK RA+++WE LYR+RV F++MT V+ E +QLL EH Sbjct: 1 MNKPSIYGLTLDQLTAWLGERGHKKFRATQVWEWLYRKRVTSFSDMTDVHPECLQLLEEH 60 Query: 61 FVFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCA 120 + T++EH KQES DGT+KFLF+L DGNLIETVLMR K+GLSVCVTTQVGCNIGCSFCA Sbjct: 61 YTILTLEEHTKQESLDGTVKFLFKLVDGNLIETVLMRHKFGLSVCVTTQVGCNIGCSFCA 120 Query: 121 SGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 SGLL KSRDLS+ EIVEQ+M+VQ HLD G +RVSH+VVMGIGEPFDN+ N+ +F+ +I Sbjct: 121 SGLLKKSRDLSAAEIVEQVMQVQLHLDRRGSSERVSHLVVMGIGEPFDNYENMSDFIRVI 180 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 KDHKGLAI + ITVSTSGLA+KI EFAD+++ VNLAISLHAPN+E+RTRIMKIN+AIPI Sbjct: 181 KDHKGLAIGPRHITVSTSGLANKIVEFADSDLGVNLAISLHAPNDEIRTRIMKINKAIPI 240 Query: 241 EKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVD 300 EKLM AIDYYLEKTNRRITLEYILLKDVND++EHALELAEL+G RR +ANVNLIPYNPVD Sbjct: 241 EKLMAAIDYYLEKTNRRITLEYILLKDVNDQREHALELAELVGERRNLANVNLIPYNPVD 300 Query: 301 EHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 EHSQYQRS +ES+ AF+DTLKKQG+S S RLEHG DIDA Sbjct: 301 EHSQYQRSTKESITAFYDTLKKQGISCSVRLEHGTDIDA 339 >Q5WKB6.1 RLMN1_BACSK Ribosomal RNA large subunit methyltransferase N 1 EC=2.1.1.- Length = 356 Score = 537 bits (1383), Expect = e-151, Method: Compositional matrix adjust. Identities = 250/339 (73%), Positives = 290/339 (85%) Query: 1 MDKQSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEH 60 M K+SIYGLT+ Q+ WL+E GHKK RA+++W+ LYR+RV FAEMT VN + +QLL +H Sbjct: 1 MSKESIYGLTMAQLTDWLMERGHKKFRATQVWDWLYRKRVTTFAEMTNVNKDCLQLLEDH 60 Query: 61 FVFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCA 120 F +TM EHV+QES DGTIKFLFRL+DGNLIETVLMR KYG SVCVTTQVGCNIGCSFCA Sbjct: 61 FAIETMSEHVRQESKDGTIKFLFRLQDGNLIETVLMRHKYGFSVCVTTQVGCNIGCSFCA 120 Query: 121 SGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 SGLL K+RDLSSGEIVEQIMKVQ HLD +G +RVSHVVVMGIGEPFDNF N ++FL II Sbjct: 121 SGLLTKNRDLSSGEIVEQIMKVQFHLDQVGKEERVSHVVVMGIGEPFDNFQNTVDFLEII 180 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 KDHKGLAI + ITVSTSGLA KI EFAD +QVNLA+SLHAPNNELR+RIMKIN+A PI Sbjct: 181 KDHKGLAIGARHITVSTSGLAHKIYEFADLKLQVNLAVSLHAPNNELRSRIMKINKAFPI 240 Query: 241 EKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVD 300 EKLM +I+YY+EKTNRR+T EYIL+KDVND +E AL+LAELIG +R ++ VNLIPYNPVD Sbjct: 241 EKLMDSINYYIEKTNRRVTYEYILIKDVNDHKEEALQLAELIGDKRHLSYVNLIPYNPVD 300 Query: 301 EHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 EHSQYQRSE E++ FFDTLKK+G++ RLEHG DIDA Sbjct: 301 EHSQYQRSEPEAISQFFDTLKKKGINCGVRLEHGTDIDA 339 >B1DCW9.1 B1DCW9_9BACL Radical SAM enzyme, Cfr family Length = 356 Score = 532 bits (1370), Expect = e-149, Method: Compositional matrix adjust. Identities = 253/340 (74%), Positives = 291/340 (85%), Gaps = 1/340 (0%) Query: 1 MDKQSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEH 60 M SIYG TL+Q+ +WL E GHKK RA+++WE LYR+RV FA+MT V+ + V+LL E+ Sbjct: 1 MSTASIYGFTLDQLTAWLGERGHKKFRATQVWEYLYRKRVTSFADMTDVHPDCVKLLEEN 60 Query: 61 FVFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCA 120 + QT+ EH KQ S DGT+K L +L DGNLIETV+MR K+GLSVCVTTQVGCNIGCSFCA Sbjct: 61 YAIQTLVEHTKQVSKDGTVKLLLKLDDGNLIETVMMRHKFGLSVCVTTQVGCNIGCSFCA 120 Query: 121 SGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 SGLL KSRDL+SGEIVEQIMK+Q HLD G G++VSH+VVMGIGEPFDNF +L +FLT + Sbjct: 121 SGLLKKSRDLTSGEIVEQIMKIQLHLDESGQGEKVSHIVVMGIGEPFDNFIHLNDFLTTV 180 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 KDHKGLAI + ITVSTSGLADKIREFAD + VNLA+SLHAPNNELRTRIMKINRAIPI Sbjct: 181 KDHKGLAIGPRHITVSTSGLADKIREFADKDQGVNLAVSLHAPNNELRTRIMKINRAIPI 240 Query: 241 EKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQ-MANVNLIPYNPV 299 EKLM AIDYYLE+TNRRITLEYILLKDVND+ EHALELAEL+G RR+ + NVNLIPYNPV Sbjct: 241 EKLMDAIDYYLERTNRRITLEYILLKDVNDQPEHALELAELVGDRRRSLVNVNLIPYNPV 300 Query: 300 DEHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 DEHSQYQRSE +S++ F+DTLKKQ VSVS RLEHG DIDA Sbjct: 301 DEHSQYQRSENDSIKGFYDTLKKQDVSVSVRLEHGADIDA 340 >B1YG36.1 RLMN_EXIS2 Ribosomal RNA large subunit methyltransferase N EC=2.1.1.- Length = 357 Score = 513 bits (1321), Expect = e-143, Method: Compositional matrix adjust. Identities = 239/339 (70%), Positives = 280/339 (82%) Query: 1 MDKQSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEH 60 M+K SIYGLTLEQM WL GHK RA ++W+ LYR+RV FAEMT VN + ++LL + Sbjct: 1 MNKPSIYGLTLEQMTEWLSHQGHKPFRAKQVWDWLYRKRVTTFAEMTNVNKDCLELLDQS 60 Query: 61 FVFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCA 120 F +M + VKQESADGTIKFLF+L DG+LIETVLMR KYGLSVCVTTQVGCNIGCSFCA Sbjct: 61 FAIDSMTQAVKQESADGTIKFLFKLYDGSLIETVLMRHKYGLSVCVTTQVGCNIGCSFCA 120 Query: 121 SGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 SGL+ KSRDLS+GEIVEQIM VQHHLD +G +RVSHVVVMGIGEPFDNF N+++FL +I Sbjct: 121 SGLIKKSRDLSAGEIVEQIMNVQHHLDAVGKEERVSHVVVMGIGEPFDNFDNMVDFLNVI 180 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 KDH GLAI + ITVSTSGLADKI +FAD +QVNLAISLHAPNNELRT+IMKINRAIP+ Sbjct: 181 KDHNGLAIGARHITVSTSGLADKIYKFADLKLQVNLAISLHAPNNELRTQIMKINRAIPL 240 Query: 241 EKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVD 300 EKLMPAIDYY++ TNR+IT+EYILL+ VND++ A+ELA+L +R + VNLIPYNPVD Sbjct: 241 EKLMPAIDYYVKTTNRKITIEYILLRGVNDQKAQAIELAKLFEDKRHLTYVNLIPYNPVD 300 Query: 301 EHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 EH QYQRS E + F+DTLKK G++ RLEHG DIDA Sbjct: 301 EHGQYQRSTSEDISTFYDTLKKNGLNCGVRLEHGTDIDA 339 >C1PD26.1 C1PD26_BACCO Radical SAM enzyme, Cfr family Length = 355 Score = 510 bits (1313), Expect = e-143, Method: Compositional matrix adjust. Identities = 243/339 (71%), Positives = 282/339 (83%), Gaps = 1/339 (0%) Query: 1 MDKQSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEH 60 M+K+SI+GLT++Q+ W++EHG KK RA ++WE LY++RVK FAEMT VN + + LL EH Sbjct: 1 MEKKSIFGLTIDQLTGWMLEHGQKKFRAQQVWEWLYQKRVKSFAEMTNVNKDCLALLEEH 60 Query: 61 FVFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCA 120 F T+ + VKQES DGT+KFLF+LKDGNLIETVLMRQ YGLSVCVTTQVGCNIGCSFCA Sbjct: 61 FHIHTLKQVVKQESKDGTVKFLFQLKDGNLIETVLMRQHYGLSVCVTTQVGCNIGCSFCA 120 Query: 121 SGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 SGLL K+RDL SGEIVEQIM VQ H D G G+RVSHVVVMGIGEPFDNF N++NFL II Sbjct: 121 SGLLRKNRDLESGEIVEQIMYVQKHFDEAGKGERVSHVVVMGIGEPFDNFENVVNFLKII 180 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 KGLAI + ITVSTSGLA KI FAD ++QVNLAISLHAPNNELR+RIMKINRA P+ Sbjct: 181 NAPKGLAIGARHITVSTSGLAPKIYAFADLDLQVNLAISLHAPNNELRSRIMKINRAYPL 240 Query: 241 EKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVD 300 EKLMPAIDYYLEKTNRRIT EYILLKDVND +E AL+LA+L+ +R +A VNLIPYNPV+ Sbjct: 241 EKLMPAIDYYLEKTNRRITFEYILLKDVNDHKEEALQLAKLLQDKRHLAYVNLIPYNPVN 300 Query: 301 EHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 EH YQRSE+ES+ F+DTLKK G++ R EHG DIDA Sbjct: 301 EHG-YQRSEKESIMTFYDTLKKNGINCVVRQEHGTDIDA 338 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,879,905,380 Number of extensions: 73547101 Number of successful extensions: 205701 Number of sequences better than 1.0e-05: 1524 Number of HSP's gapped: 200613 Number of HSP's successfully gapped: 1536 Length of query: 339 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 203 Effective length of database: 1,779,438,163 Effective search space: 361225947089 Effective search space used: 361225947089 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)