BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0003.p (180 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8L651.1 A8L651_FRASN Transcriptional regulator, TetR family 163 7e-39 C1R8N7.1 C1R8N7_9ACTO Transcriptional regulator, TetR family 144 3e-33 Q846X8.1 Q846X8_STRCM Putative monensin cluster negative regulator 140 5e-32 C4E2Z5.1 C4E2Z5_STRRS Transcriptional regulator, TetR family 127 3e-28 B5H4V5.1 B5H4V5_STRPR TetR-family transcriptional regulator 118 2e-25 >A8L651.1 A8L651_FRASN Transcriptional regulator, TetR family Length = 200 Score = 163 bits (412), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 77/162 (47%), Positives = 112/162 (69%) Query: 2 ASIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFE 61 A+IQ HAL+LFR+ GY ATTVEQI +AAE+S +TFFRYF TKE+V+L D+ DPL++ +F Sbjct: 22 AAIQTHALRLFREQGYNATTVEQIIDAAEVSETTFFRYFPTKEEVVLQDDLDPLIIEAFH 81 Query: 62 SQPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLII 121 +QPA LTP+ A+R A+ +TP + ++ER L+++ P LRAAM++ + M L+ Sbjct: 82 NQPADLTPIQAIRAAIRQQFDALTPDQRQAIQERTVLIVAEPALRAAMLDQISQTMLLLA 141 Query: 122 ELTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEHPEADF 163 + RTGR ++DLAVR AG+ IG ++ + A+ P AD Sbjct: 142 DAIAERTGRPKDDLAVRTVAGSAIGAMLATLPALADDPNADL 183 >C1R8N7.1 C1R8N7_9ACTO Transcriptional regulator, TetR family Length = 214 Score = 144 bits (363), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 72/161 (44%), Positives = 102/161 (63%) Query: 2 ASIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFE 61 A+IQ HAL+LFR+ GY ATT+EQIA AA++S ST +RYF TKED++L D+YDPLL A+F Sbjct: 27 AAIQRHALRLFREAGYSATTMEQIAAAADVSPSTLYRYFPTKEDLILQDDYDPLLEAAFR 86 Query: 62 SQPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLII 121 +QP L L A R A+ ++ P + + ER + ++PE+RA +++M D+I Sbjct: 87 AQPPDLPILEAFRTAVNEAMSQVPPTDQAEALERARMAYTIPEVRARALDHMFTTTDMIA 146 Query: 122 ELTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEHPEAD 162 L R R +DL VR AG VIG ++ Q + P+AD Sbjct: 147 TLFAERLNRTSDDLEVRVLAGTVIGAVMAAQQRWISDPDAD 187 >Q846X8.1 Q846X8_STRCM Putative monensin cluster negative regulator Length = 191 Score = 140 bits (353), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 72/162 (44%), Positives = 105/162 (64%) Query: 2 ASIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFE 61 A+IQ A++LFR+ GY ATT+EQIAEAAE++ ST FRYF+TK+D++ +YD + Sbjct: 14 AAIQREAVRLFREQGYTATTIEQIAEAAEVAPSTVFRYFATKQDLVFSHDYDLPFAMMVQ 73 Query: 62 SQPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLII 121 +Q LTP+ A R A+ S ++++ QEL+ RER L++S PEL A + N+ M ++ Sbjct: 74 AQSPDLTPIQAERQAIRSMLQDISEQELALQRERFVLILSEPELWGASLGNIGQTMQIMS 133 Query: 122 ELTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEHPEADF 163 E +R GR+ D AVRA+ GAV GV + V +A P+ DF Sbjct: 134 EQVAKRAGRDPRDPAVRAYTGAVFGVMLQVSMDWANDPDMDF 175 >C4E2Z5.1 C4E2Z5_STRRS Transcriptional regulator, TetR family Length = 199 Score = 127 bits (320), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 73/155 (47%), Positives = 97/155 (62%), Gaps = 2/155 (1%) Query: 3 SIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFES 62 +IQ HAL+LF + GY+ATTVEQIAEAAEIS STFFRYF KED +L D+Y P+L A + Sbjct: 21 TIQEHALRLFAEQGYEATTVEQIAEAAEISPSTFFRYFPAKEDAVLQDDYAPVLTAMLAA 80 Query: 63 QPAHLTPLMALRNAMLSGAKEMTP-QELSTMRERNELVMSVPELRAAMINNMVNMMDLII 121 QPA L P+ ALR A + GA E P +E + R L +S LRA ++NM+ +D++ Sbjct: 81 QPADLEPVPALR-AAVRGAFERFPREEEPQILARTRLQLSHAALRAKTVDNMIGTIDMLA 139 Query: 122 ELTCRRTGREREDLAVRAFAGAVIGVNISVMQYYA 156 E RR G + D AGAV+G + + +A Sbjct: 140 EGVARRRGLDAPDALSLTTAGAVVGALLPALFGWA 174 >B5H4V5.1 B5H4V5_STRPR TetR-family transcriptional regulator Length = 209 Score = 118 bits (296), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 64/160 (40%), Positives = 87/160 (54%) Query: 3 SIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFES 62 +I+ +L + GY ATTVEQIA AAE+S ST FRYF TKED++L D YDP++ A + Sbjct: 31 AIRRATYRLIAEQGYDATTVEQIAAAAEVSPSTVFRYFPTKEDIVLTDEYDPVMEAELRA 90 Query: 63 QPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLIIE 122 +PA PL +LR + + E M R L++ VP LRA M M + L+ Sbjct: 91 RPADEPPLASLRIILTGAVAAILENEPEEMHLRTRLMLEVPALRARMTETMADTSQLLAR 150 Query: 123 LTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEHPEAD 162 R GRE +DL VR F AV+ + ++ EH D Sbjct: 151 PIADRVGREPDDLEVRVFTAAVMAALREALIHWGEHGRRD 190 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.133 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 820,825,688 Number of extensions: 27399467 Number of successful extensions: 91608 Number of sequences better than 1.0e-05: 197 Number of HSP's gapped: 91620 Number of HSP's successfully gapped: 197 Length of query: 180 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 53 Effective length of database: 1,864,227,298 Effective search space: 98804046794 Effective search space used: 98804046794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)