BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0001.p (449 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCD3.1 B1DCD3_9BACL Polysaccharide deacetylase 309 3e-82 B3K8E3.1 B3K8E3_9BACI Polysaccharide deacetylase 153 3e-35 Q8RBF4.1 Q8RBF4_THETN Predicted xylanase/chitin deacetylase 129 6e-28 B7R6J7.1 B7R6J7_9THEO Polysaccharide deacetylase family sporulat... 129 6e-28 A5KSU1.1 A5KSU1_9BACT Polysaccharide deacetylase (Fragment) 125 1e-26 >B1DCD3.1 B1DCD3_9BACL Polysaccharide deacetylase Length = 432 Score = 309 bits (792), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 177/459 (38%), Positives = 247/459 (53%), Gaps = 42/459 (9%) Query: 4 LEQQAALIVELLSLEHKQDGY-QLEVGLTGSGGFTRKVLLIDEFTYLQMNMLASSQGHRV 62 +E + ++ELLSL G Q+++G+T + V+ +DEFT+L++ L G R+ Sbjct: 3 METYSTQVLELLSLGRTSSGISQMKIGVTQDDKYATYVIEVDEFTFLELEALGPLHGDRI 62 Query: 63 RLSLYPKWDPFRRTYFSSLIKMNSTYSETLYFACSEAYVSQLLQLKQLTFPP---PEADA 119 R+S Y KWDP+R +Y+SS I+ + LYFACSE Y++ + Q++Q P + D Sbjct: 63 RISPYSKWDPYRNSYYSSFIRTTGSTRVPLYFACSEDYIAMIRQIRQGELSPVSPAKEDQ 122 Query: 120 VPTELPSPEPQQTGPLTRSAGTFRLARPIVLRCIXXXXXXXXXXXRMDGTVFNSAE--AH 177 V + P P + P RS R+ P V I G +SA Sbjct: 123 VVQAILMPVPNRPDP--RSGFASRM--PKVAVAILIMIGLLAILSIATGGKVDSARLLGG 178 Query: 178 ENFNEQELSARSSVPVAYVPE-------IHQASFQGTEAVPVPETGQVLEPEGXXXXXXX 230 + + + S +PVAY H + T PVP V +PE Sbjct: 179 QVAKAEVTNGNSGIPVAYASSSVNNTAITHDTAAVVTADQPVPAQNPV-QPESRE----- 232 Query: 231 XXXXXXXXXXXXXXXXXXXQSPLVETLELDGSGYEYSLAKGYVALSFDDGPSKYTQEIVD 290 + P + + +D + L K YVAL+FDDGPSK T++IVD Sbjct: 233 -------------------EKPAFKIIAIDEDKKFFGLPKEYVALTFDDGPSKLTKDIVD 273 Query: 291 ILLAHGVAANFLFIGQNAQHYPQAVRYADEHGMPVGSHSWDHSDLTKNSSGENHNNLLRS 350 IL V A FLF+GQN H+ +AV YA HGM +G+HSWDHS LTK++ E + NL ++ Sbjct: 274 ILTEQNVPATFLFVGQNVAHHKEAVTYAAGHGMSIGNHSWDHSVLTKSTLQEQNENLSKA 333 Query: 351 VQVLEQNTGKPVTVFRPPYGAINGSLAAEAGKQKLKVLLWNRDPEDWKADTAEQITRYFH 410 + +E T KPVT+FRPPYGA+N L + A KQ++K LLWNRDPEDW A T E IT YFH Sbjct: 334 SKAIEALTHKPVTLFRPPYGAVNDDLVSAADKQQMKTLLWNRDPEDWNAKTPEDITAYFH 393 Query: 411 NTDPSGGIYLLHEKAVTVKALPAIIEYLKSKQLKFAIFR 449 + + +GGIY++HE TV+ALPAII+YLK K L F F+ Sbjct: 394 HVEAAGGIYVMHEDKYTVQALPAIIKYLKGKDLTFVAFK 432 >B3K8E3.1 B3K8E3_9BACI Polysaccharide deacetylase Length = 426 Score = 153 bits (387), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 75/178 (42%), Positives = 106/178 (59%) Query: 266 YSLAKGYVALSFDDGPSKYTQEIVDILLAHGVAANFLFIGQNAQHYPQAVRYADEHGMPV 325 YS+ KGYVAL+FDDGPSK++ +I+++L H V F F+G N + +P V+ +G V Sbjct: 243 YSIPKGYVALTFDDGPSKHSMDIMNVLKKHEVGGTFFFVGTNVKKHPDYVKAIHANGYSV 302 Query: 326 GSHSWDHSDLTKNSSGENHNNLLRSVQVLEQNTGKPVTVFRPPYGAINGSLAAEAGKQKL 385 GSHS HS + + E L R+ ++E T + + +FRPPYGA + A + +Q Sbjct: 303 GSHSMTHSQMPSLTYEEQKAELTRAADLIEHITDEKLVLFRPPYGAFDDRTEALSRQQGN 362 Query: 386 KVLLWNRDPEDWKADTAEQITRYFHNTDPSGGIYLLHEKAVTVKALPAIIEYLKSKQL 443 KV+LWNRDPEDW +I Y + + SG I LLHE V ALP II+ L+ + L Sbjct: 363 KVVLWNRDPEDWLTRDEGKILDYVQHIEASGSIILLHESQAVVDALPKIIKSLQDQGL 420 >Q8RBF4.1 Q8RBF4_THETN Predicted xylanase/chitin deacetylase Length = 324 Score = 129 bits (324), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 69/176 (39%), Positives = 105/176 (59%), Gaps = 2/176 (1%) Query: 273 VALSFDDGP-SKYTQEIVDILLAHGVAANFLFIGQNAQHYPQAVRYADEHGMPVGSHSWD 331 VAL+FDDGP YT++ VDIL + V A F IG++A+ +P+ ++Y E+G +G HS+ Sbjct: 139 VALTFDDGPFPIYTEKYVDILKSMDVKATFFVIGKHAEKHPELLKYIVENGNEIGLHSYS 198 Query: 332 HSDLTKNSSGENHNNLLRSVQVLEQNTGKPVTVFRPPYGAINGSLAAEAGKQKLKVLLWN 391 H ++ K + L ++ Q++ + TG T+FRPP+GA N +L + LKV+LWN Sbjct: 199 HFNMKKLKPEKMVEELYKTQQIIVEATGIKPTLFRPPFGAYNSTLIEISNALGLKVVLWN 258 Query: 392 RDPEDWKADTAEQITRYFHNTDPSGGIYLLHE-KAVTVKALPAIIEYLKSKQLKFA 446 DP+DW+ + E + + G I L+HE K T+ ALP II+ LK + KF Sbjct: 259 VDPDDWRNPSVESVVNRVLSHTRDGSIILMHEGKPSTLAALPQIIKKLKEEGYKFV 314 >B7R6J7.1 B7R6J7_9THEO Polysaccharide deacetylase family sporulation protein PdaB, putative Length = 324 Score = 129 bits (324), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 69/176 (39%), Positives = 105/176 (59%), Gaps = 2/176 (1%) Query: 273 VALSFDDGP-SKYTQEIVDILLAHGVAANFLFIGQNAQHYPQAVRYADEHGMPVGSHSWD 331 VAL+FDDGP YT++ VDIL + V A F IG++A+ +P+ ++Y E+G +G HS+ Sbjct: 139 VALTFDDGPFPIYTEKYVDILKSMDVKATFFVIGKHAEKHPELLKYIVENGNEIGLHSYS 198 Query: 332 HSDLTKNSSGENHNNLLRSVQVLEQNTGKPVTVFRPPYGAINGSLAAEAGKQKLKVLLWN 391 H ++ K + L ++ Q++ + TG T+FRPP+GA N +L + LKV+LWN Sbjct: 199 HFNMKKLKPEKMVEELYKTQQIIVEATGIKPTLFRPPFGAYNSTLIEISNALGLKVVLWN 258 Query: 392 RDPEDWKADTAEQITRYFHNTDPSGGIYLLHE-KAVTVKALPAIIEYLKSKQLKFA 446 DP+DW+ + E + + G I L+HE K T+ ALP II+ LK + KF Sbjct: 259 VDPDDWRNPSVESVVNRVLSHTRDGSIILMHEGKPSTLAALPQIIKKLKEEGYKFV 314 >A5KSU1.1 A5KSU1_9BACT Polysaccharide deacetylase (Fragment) Length = 287 Score = 125 bits (314), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 65/175 (37%), Positives = 98/175 (56%), Gaps = 1/175 (0%) Query: 268 LAKGYVALSFDDGPSKYTQEIVDILLAHGVAANFLFIGQNAQHYPQAVRYADEHGMPVGS 327 + G V L+FDDGP +T++++D+L + V A F IG+N Q YP ++ G +G+ Sbjct: 109 MGTGGVHLTFDDGPGAHTEQVLDVLSRYNVHATFYVIGRNVQRYPGTMQRIKNEGHTIGN 168 Query: 328 HSWDHSDLTKNSSGENHNNLLRSVQVLEQNTGKPVTVFRPPYGAINGSLAAEAGKQKLKV 387 H++ H+DLT S L + +EQ G + FRPPYGA NG + + A + V Sbjct: 169 HTFTHADLTVLSEAGVRQELASTQAAIEQACGVTPSAFRPPYGAENGIVRSIAASMGMSV 228 Query: 388 LLWNRDPEDWKADTAEQITRYFHNTDPSGGIYLLHE-KAVTVKALPAIIEYLKSK 441 LW+ DP DW A + +IT+ T G + LLH TV ALP+IIE ++++ Sbjct: 229 DLWSVDPNDWAAPGSSEITQRVLATTRPGAVVLLHVLNQQTVDALPSIIEGIRAQ 283 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,526,054,707 Number of extensions: 99242476 Number of successful extensions: 307743 Number of sequences better than 1.0e-05: 2126 Number of HSP's gapped: 306018 Number of HSP's successfully gapped: 2126 Length of query: 449 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 310 Effective length of database: 1,751,175,118 Effective search space: 542864286580 Effective search space used: 542864286580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)