BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0008.p (594 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZCP0.1 C0ZCP0_BREBN Peptidase M3 family protein 518 e-145 A8MKX5.1 A8MKX5_ALKOO Oligoendopeptidase, pepF/M3 family 516 e-144 A6TM57.1 A6TM57_ALKMQ Oligoendopeptidase, pepF/M3 family 506 e-141 A6LRH7.1 A6LRH7_CLOB8 Oligoendopeptidase, pepF/M3 family 500 e-139 B2TMP2.1 B2TMP2_CLOBB Oligoendopeptidase F 488 e-135 >C0ZCP0.1 C0ZCP0_BREBN Peptidase M3 family protein Length = 595 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 255/593 (43%), Positives = 376/593 (63%), Gaps = 6/593 (1%) Query: 1 MELTWELDSLYPSFQSEKYWQDRRLLTEYTERLIEWAAGHFQNRTQNEKELAQAIEQFLK 60 M + W LD LY SF + ++ +D TE + WAA QN +E +A+ +E+++ Sbjct: 1 MNMRWNLDVLYSSFDAPEWKRDWEKFVRETEEIKSWAA---QNLQSSEDAVAK-MEKYIT 56 Query: 61 QYNAYKRVYHCLFSYAELRFSLDRSDAEAMNRMDELEDXXXXXXXXXXXFSKWLAGVADL 120 + + L++Y EL S+D + A+ ++++++ F KWL +++L Sbjct: 57 MMTSVLQTQFRLYAYGELTASVDAKNEAALLAIEKVQNQAVELVEPGVQFQKWLGSLSNL 116 Query: 121 KAVIASSAYLAGHEFYLYGLQRQSEHLLGEEAESVIARMQSTGSKAWERLYMRTLSMLRM 180 +I S L H F L + +S+++LGE+ E ++A+M++TGS AW +L+ S L + Sbjct: 117 DELITQSELLETHRFMLTEMSDKSKYMLGEKEEIILAKMKNTGSNAWTKLHEMLTSTLLV 176 Query: 181 DVQIGGQARSLSLAELQNLAYDGDAGVRKAAYEAEREACRSVEEQSAACLNSVSGEALAI 240 D+ + G+ + L L ++N+A + D +RK AYEAE A + + + +AA LN + GE + + Sbjct: 177 DITVDGEHKQLPLPVVRNMANEKDPQLRKTAYEAELAAYKKIVDAAAASLNGIKGEVITV 236 Query: 241 YGLRGYASPLHKVLKASRMNRKTLEVMLEAIEESLPFFHQYYMKKAALLGH-SGPLPFYD 299 LRGY SPL K L+ SRM+R+TLE ML+A+ ESLP FHQY+ KA LLGH S LPFYD Sbjct: 237 AKLRGYESPLDKTLQDSRMDRETLEAMLQAMRESLPSFHQYFRTKAKLLGHESAQLPFYD 296 Query: 300 VFAPLAEESSAKITYPEAGDVIVSGFSAFSPELGEFARKVFRQRWIDAEPREGKGNFGMC 359 +FAP+ E + + +Y E+ D IV F +FS +L +A + + +WIDAE REGK C Sbjct: 297 LFAPVGE-ADMRFSYEESRDFIVKHFGSFSEKLANYAARAYDNQWIDAEIREGKRGGAFC 355 Query: 360 VDIFPIGESRIITSFTGNYIDVSVLAHEIGHAYHSSCLAGETMVNTDYPVPVAETASIFC 419 ++ +GESRI++++TG+Y DVS +AHE+GH YH CL E +N+DYP+P+AETASI C Sbjct: 356 YNLHIVGESRIMSNYTGSYSDVSTMAHELGHGYHGECLVNEAFLNSDYPMPIAETASILC 415 Query: 420 ESLIHEELLNMAAESEALAIRERSLSDAGYYIVDFYARYLFESRLYERRQSGPLSAHELN 479 E++I L A EA AI E +S A IVD Y+RYLFE+ ++ERR G LS LN Sbjct: 416 ETIIANAALEQATSEEAFAILENDISGASQVIVDIYSRYLFETAVFERRTEGALSVSTLN 475 Query: 480 DLMHESMVAAYGGSVDPATFHPYQWISKAGYYMAGNEFLNFPYSFGLLFSKGLYAQYKKQ 539 +LM ++ AYG +DP HPY W+ K YY A F NFPY+FGLLF+KGLYA+Y K+ Sbjct: 476 ELMLQAQKDAYGDGLDPEALHPYMWVCKPHYYSADYNFYNFPYAFGLLFAKGLYAEYLKR 535 Query: 540 GGDFAERYRSFLAATSKNTIADAAGLMNIDVDSPQFWRDALALIAEDIQEFVE 592 G F E+Y L+ T K +IAD A +M++DV S FWR++L+L+A+DI++FVE Sbjct: 536 GEAFVEQYDKLLSVTGKMSIADVAAIMDVDVRSVDFWRNSLSLVAKDIEKFVE 588 >A8MKX5.1 A8MKX5_ALKOO Oligoendopeptidase, pepF/M3 family Length = 592 Score = 516 bits (1329), Expect = e-144, Method: Compositional matrix adjust. Identities = 260/590 (44%), Positives = 368/590 (62%), Gaps = 5/590 (0%) Query: 3 LTWELDSLYPSFQSEKYWQDRRLLTEYTERLIEWAAGHFQNRTQNEKELAQAIEQFLKQY 62 + W L+ LY SF S ++ QD L+ + I+W FQN T+N KE IEQ++++ Sbjct: 1 MRWSLNELYTSFDSLEFLQDMDKLSRFIPEFIQWVKQEFQN-TKNPKE---KIEQYIEKQ 56 Query: 63 NAYKRVYHCLFSYAELRFSLDRSDAEAMNRMDELEDXXXXXXXXXXXFSKWLAGVADLKA 122 + Y L SY+ L S D +A A+ D+L+ F KWL V DL+ Sbjct: 57 QEFTETYSRLMSYSYLTASTDAKNATALKIQDQLQSKASDLTEATVIFEKWLGTVNDLEK 116 Query: 123 VIASSAYLAGHEFYLYGLQRQSEHLLGEEAESVIARMQSTGSKAWERLYMRTLSMLRMDV 182 +I SS L H+F+L + +++LL EE E +I++M +G++AW +L S L +D+ Sbjct: 117 IIESSTILKEHQFFLKEILLNNQYLLSEEEELLISKMSQSGARAWAKLQALLASTLLVDI 176 Query: 183 QIGGQARSLSLAELQNLAYDGDAGVRKAAYEAEREACRSVEEQSAACLNSVSGEALAIYG 242 I G + L L ++N+AY +A +RK AYEAE ++ +EE S A LN + GEAL + Sbjct: 177 TIDGVEKQLPLPMVRNMAYSSNADLRKIAYEAELKSYAKIEESSGAALNGIKGEALTLSE 236 Query: 243 LRGYASPLHKVLKASRMNRKTLEVMLEAIEESLPFFHQYYMKKAALLGHSGPLPFYDVFA 302 RG+ASPL + + SRMN +TL+ ML A++ESLPFFH+YY KK LLGHS LPFYD+FA Sbjct: 237 KRGFASPLEQTVIGSRMNTETLDSMLAAMKESLPFFHKYYRKKGELLGHSNGLPFYDLFA 296 Query: 303 PLAEESSAKITYPEAGDVIVSGFSAFSPELGEFARKVFRQRWIDAEPREGKGNFGMCVDI 362 P+ + TY EA I+ F+ FS LG FA+K F WIDAEPR+GK C ++ Sbjct: 297 PVGNVNMV-FTYEEAQKYIIENFNTFSQALGNFAQKAFDNNWIDAEPRDGKRGGAFCFNL 355 Query: 363 FPIGESRIITSFTGNYIDVSVLAHEIGHAYHSSCLAGETMVNTDYPVPVAETASIFCESL 422 PI ESRI+ +F G++ +++ LAHE+GH YH CL +++N+ YP+P+AETASIF E++ Sbjct: 356 HPIKESRILANFDGSFSNMTTLAHELGHGYHGHCLKDASILNSSYPMPLAETASIFAETI 415 Query: 423 IHEELLNMAAESEALAIRERSLSDAGYYIVDFYARYLFESRLYERRQSGPLSAHELNDLM 482 I L A EA I E S+SDAG IVD Y+R+LFES +++RR++ LS +E+ ++M Sbjct: 416 IINSALKNATPEEAFGILETSISDAGQVIVDIYSRFLFESEVFKRRKNASLSVNEIKEIM 475 Query: 483 HESMVAAYGGSVDPATFHPYQWISKAGYYMAGNEFLNFPYSFGLLFSKGLYAQYKKQGGD 542 ++ AYG +D H WI+K+ YY A F NFPY+FGLLFSKGLY++Y K+G Sbjct: 476 IDAQKQAYGDGLDHNVLHDSMWINKSHYYSADRHFYNFPYAFGLLFSKGLYSEYLKRGES 535 Query: 543 FAERYRSFLAATSKNTIADAAGLMNIDVDSPQFWRDALALIAEDIQEFVE 592 F + Y + L AT KN I D A +MNIDV S FWR +L L+ +DI+ F+E Sbjct: 536 FVKEYDALLEATGKNNIVDVAKVMNIDVTSIDFWRSSLGLVQKDIERFIE 585 >A6TM57.1 A6TM57_ALKMQ Oligoendopeptidase, pepF/M3 family Length = 589 Score = 506 bits (1302), Expect = e-141, Method: Compositional matrix adjust. Identities = 245/591 (41%), Positives = 361/591 (61%), Gaps = 5/591 (0%) Query: 1 MELTWELDSLYPSFQSEKYWQDRRLLTEYTERLIEWAAGHFQNRTQNEKELAQAIEQFLK 60 M L W L LY SF+ E++ +D + + + E + +W+ Q + +E+ + Sbjct: 1 MNLYWSLKELYQSFEDEQFKRDLKQVDNFIETMDQWS----QTELTTHEHAISKLEEGIN 56 Query: 61 QYNAYKRVYHCLFSYAELRFSLDRSDAEAMNRMDELEDXXXXXXXXXXXFSKWLAGVADL 120 Q A V+ L+++ L S+D + +A +D L+ F +W+ G+ +L Sbjct: 57 QSQALDHVFSKLYAFTVLTLSVDAKNNQAQQVLDSLQQKETKLTKSIVTFERWIGGLDNL 116 Query: 121 KAVIASSAYLAGHEFYLYGLQRQSEHLLGEEAESVIARMQSTGSKAWERLYMRTLSMLRM 180 +I SS L H F+L + ++++LL +E E +I++M++TGSKAW +L S L + Sbjct: 117 SELIVSSDTLKEHHFFLSEIHDKNQYLLSDEEEILISKMKNTGSKAWSKLQELLTSTLLI 176 Query: 181 DVQIGGQARSLSLAELQNLAYDGDAGVRKAAYEAEREACRSVEEQSAACLNSVSGEALAI 240 ++ + G+ + L L ++N+++D D VRK+A+EAE +A +EE SAA LN + GE + Sbjct: 177 EINVDGETKKLPLPVVRNMSHDSDVSVRKSAFEAELKAYGDIEESSAAALNGIKGEVFTL 236 Query: 241 YGLRGYASPLHKVLKASRMNRKTLEVMLEAIEESLPFFHQYYMKKAALLGHSGPLPFYDV 300 LRGY SPL + L +RM L+ MLEA+ ESLP F +YY +KAALLGH G LPFYD+ Sbjct: 237 SELRGYDSPLAETLIQNRMESNVLDTMLEAMRESLPSFQRYYRRKAALLGHQGGLPFYDL 296 Query: 301 FAPLAEESSAKITYPEAGDVIVSGFSAFSPELGEFARKVFRQRWIDAEPREGKGNFGMCV 360 AP+ + K +Y EA D IV+ F +FS EL +FA F +WIDAEPR+GK C Sbjct: 297 LAPMGS-VNKKFSYEEARDYIVTNFRSFSDELADFAHHAFESQWIDAEPRQGKRGGAFCF 355 Query: 361 DIFPIGESRIITSFTGNYIDVSVLAHEIGHAYHSSCLAGETMVNTDYPVPVAETASIFCE 420 ++ PI ESRI+ +FTG + DV+ LAHE+GHAYH CL E+++N++YP+P+AETASIF E Sbjct: 356 NLHPIKESRILANFTGTFSDVTTLAHELGHAYHGYCLKDESILNSNYPMPIAETASIFAE 415 Query: 421 SLIHEELLNMAAESEALAIRERSLSDAGYYIVDFYARYLFESRLYERRQSGPLSAHELND 480 S++ L A + E L + E S+S AG IVD Y+RYLFE+ ++E+R+ LS E + Sbjct: 416 SVVVNAALKEANDEEKLNLLEESISSAGQVIVDIYSRYLFETAVFEKRRDYSLSVKEFKE 475 Query: 481 LMHESMVAAYGGSVDPATFHPYQWISKAGYYMAGNEFLNFPYSFGLLFSKGLYAQYKKQG 540 LM + AYG +D HPY W++K YY F NFPY+FGLLF+KGLYA+Y QG Sbjct: 476 LMVSAQKEAYGDGLDHRVLHPYMWVNKPHYYSGSRSFYNFPYAFGLLFAKGLYAEYLIQG 535 Query: 541 GDFAERYRSFLAATSKNTIADAAGLMNIDVDSPQFWRDALALIAEDIQEFV 591 F +Y L AT K I D ++ IDV S +FWR++L L+ DI++F+ Sbjct: 536 DAFIPKYNELLTATGKLPIIDVGKMVGIDVTSIEFWRNSLKLVERDIEQFI 586 >A6LRH7.1 A6LRH7_CLOB8 Oligoendopeptidase, pepF/M3 family Length = 591 Score = 500 bits (1288), Expect = e-139, Method: Compositional matrix adjust. Identities = 243/592 (41%), Positives = 356/592 (60%), Gaps = 5/592 (0%) Query: 1 MELTWELDSLYPSFQSEKYWQDRRLLTEYTERLIEWAAGHFQNRTQNEKELAQAIEQFLK 60 MEL W L +YPSF +++ QD LT+ E + +WA T+N+ L + +E ++ Sbjct: 1 MELNWSLKEIYPSFDGDEFKQDLNKLTDIIEEINKWAL----EATKNKNNLVEKLEDYIN 56 Query: 61 QYNAYKRVYHCLFSYAELRFSLDRSDAEAMNRMDELEDXXXXXXXXXXXFSKWLAGVADL 120 ++ + + + L S++ D A+ D LE F ++++ + L Sbjct: 57 RFTKVTDLSSRISIFINLSLSVNTKDKNALRYSDILEKKLTNLIGSSVKFERYISSIEGL 116 Query: 121 KAVIASSAYLAGHEFYLYGLQRQSEHLLGEEAESVIARMQSTGSKAWERLYMRTLSMLRM 180 +I+ S L HEF L + QS++LL ++ ES+IA M++TGS AW +L +S L + Sbjct: 117 DEIISKSKLLKEHEFMLKNIVEQSQYLLSDKEESIIANMKNTGSNAWAKLKDNLISTLLV 176 Query: 181 DVQIGGQARSLSLAELQNLAYDGDAGVRKAAYEAEREACRSVEEQSAACLNSVSGEALAI 240 +++ + + + L + N+AYD DA VRK AYEAE ++ + VEE AA LN + GE L + Sbjct: 177 EIEEDNEIKHIPLTMVLNMAYDKDATVRKKAYEAEIKSYKKVEEGVAAALNGIKGEVLTV 236 Query: 241 YGLRGYASPLHKVLKASRMNRKTLEVMLEAIEESLPFFHQYYMKKAALLGHSGPLPFYDV 300 RGY SPL K L SRM+ ++LE M A++ESLP F +Y +K +LGH LPFYD+ Sbjct: 237 CDFRGYKSPLEKTLIDSRMSEESLEAMFLAMKESLPVFRKYLRRKGEMLGHKNGLPFYDL 296 Query: 301 FAPLAEESSAKITYPEAGDVIVSGFSAFSPELGEFARKVFRQRWIDAEPREGKGNFGMCV 360 +AP+ E + K TY E + F FS LG+FARK WID +P+EGK C Sbjct: 297 YAPVCE-ADMKFTYEEGTKFVEKNFRTFSDNLGDFARKAIDNHWIDVKPKEGKVGGAFCE 355 Query: 361 DIFPIGESRIITSFTGNYIDVSVLAHEIGHAYHSSCLAGETMVNTDYPVPVAETASIFCE 420 ++ GESRI+ ++ N+ DV LAHE+GH +H CL ET +N+DYP+P+AETAS FCE Sbjct: 356 NLHFNGESRILLNYGDNFGDVVTLAHELGHGFHGECLNNETTLNSDYPMPIAETASTFCE 415 Query: 421 SLIHEELLNMAAESEALAIRERSLSDAGYYIVDFYARYLFESRLYERRQSGPLSAHELND 480 ++I + + A+++EALAI E +SD IVD Y+R+LFE +E R+ LS E+ D Sbjct: 416 TIIKKAAIKDASKNEALAILETEISDCTQVIVDIYSRFLFEKSFFEARKGSSLSVEEIKD 475 Query: 481 LMHESMVAAYGGSVDPATFHPYQWISKAGYYMAGNEFLNFPYSFGLLFSKGLYAQYKKQG 540 LM ++ AYG +DP HPY W K YY A + NFPY+FGLLF+KGLYA+Y K+G Sbjct: 476 LMLDAQREAYGDGLDPDFLHPYMWTWKPHYYDAEYNYYNFPYAFGLLFAKGLYAEYLKKG 535 Query: 541 GDFAERYRSFLAATSKNTIADAAGLMNIDVDSPQFWRDALALIAEDIQEFVE 592 F++ Y L+ T KN IAD A +M ID++ +FWR++L I EDI+EF++ Sbjct: 536 KTFSKEYEKLLSITGKNKIADVARVMGIDINDTEFWRNSLKTIEEDIEEFID 587 >B2TMP2.1 B2TMP2_CLOBB Oligoendopeptidase F Length = 593 Score = 488 bits (1255), Expect = e-135, Method: Compositional matrix adjust. Identities = 236/592 (39%), Positives = 353/592 (59%), Gaps = 5/592 (0%) Query: 1 MELTWELDSLYPSFQSEKYWQDRRLLTEYTERLIEWAAGHFQNRTQNEKELAQAIEQFLK 60 MEL W L +YPSF SE++ D L E + EW+ T +++ L +E+++K Sbjct: 1 MELNWSLKEIYPSFDSEEFKSDLESLKYTIELINEWS----NEVTIDKENLVFKLEEYIK 56 Query: 61 QYNAYKRVYHCLFSYAELRFSLDRSDAEAMNRMDELEDXXXXXXXXXXXFSKWLAGVADL 120 ++ + + L Y L S+D +D E + D +E +W++ + ++ Sbjct: 57 KFTKFTDLASKLSIYINLVLSVDTTDKEGLKYSDVIEKKLTEIVESSTKLERWISSIENI 116 Query: 121 KAVIASSAYLAGHEFYLYGLQRQSEHLLGEEAESVIARMQSTGSKAWERLYMRTLSMLRM 180 VI+ S L HEF L + S++LL ++ E++IA M++TGS AW +L + +S L++ Sbjct: 117 DEVISKSRLLKDHEFILKNIIENSKYLLSDKEENIIASMKNTGSNAWLKLKDKLISNLKV 176 Query: 181 DVQIGGQARSLSLAELQNLAYDGDAGVRKAAYEAEREACRSVEEQSAACLNSVSGEALAI 240 D++ G+ +SL L + N+AYD D+ VRK AYEAE + + VEE AA LN + GE L + Sbjct: 177 DIEENGEIKSLPLTLVLNMAYDKDSTVRKKAYEAEIASYKKVEEGVAAALNGIKGEVLTV 236 Query: 241 YGLRGYASPLHKVLKASRMNRKTLEVMLEAIEESLPFFHQYYMKKAALLGHSGPLPFYDV 300 LRGY SPL + LK SRM+ ++L+ M+ A++ESLP F +Y +KA +LG+ LPFY++ Sbjct: 237 CDLRGYKSPLEQTLKDSRMDEESLDAMISAMKESLPIFRKYLRRKAEILGYKNGLPFYEL 296 Query: 301 FAPLAEESSAKITYPEAGDVIVSGFSAFSPELGEFARKVFRQRWIDAEPREGKGNFGMCV 360 +AP+ ++ K Y E +V F+ FS L FA+K WID P+ GK C Sbjct: 297 YAPI-NDADMKFNYEEGTKFVVKNFNTFSNNLANFAKKAIDNSWIDVMPKSGKVGGAFCE 355 Query: 361 DIFPIGESRIITSFTGNYIDVSVLAHEIGHAYHSSCLAGETMVNTDYPVPVAETASIFCE 420 ++ I ESR + ++ ++ DV LAHE+GH +H CL ET +N+DYP+P+AETAS FCE Sbjct: 356 NLHFIDESRFLLNYGESFGDVVTLAHELGHGFHGECLNNETTLNSDYPMPIAETASTFCE 415 Query: 421 SLIHEELLNMAAESEALAIRERSLSDAGYYIVDFYARYLFESRLYERRQSGPLSAHELND 480 ++I + + A + EAL+I E +SD IVD Y+R+LFE +E R+ LS E+ + Sbjct: 416 TIIKKAAIKEATKDEALSILETEISDCTQVIVDIYSRFLFEKSFFEARKESALSVEEIKE 475 Query: 481 LMHESMVAAYGGSVDPATFHPYQWISKAGYYMAGNEFLNFPYSFGLLFSKGLYAQYKKQG 540 LM S + AYG +D HPY W K YY A + F NFPY+FGLLF+KGLYA+Y K+G Sbjct: 476 LMLASQIEAYGDGLDKNYLHPYMWTWKPHYYYADSNFYNFPYAFGLLFAKGLYAEYLKRG 535 Query: 541 GDFAERYRSFLAATSKNTIADAAGLMNIDVDSPQFWRDALALIAEDIQEFVE 592 F+ Y L+ T KN IAD +M ID+ +FW ++L I EDI+EF++ Sbjct: 536 ESFSNEYEKLLSITGKNKIADVTKVMGIDIHDKKFWGNSLKTIEEDIEEFMK 587 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,380,719,693 Number of extensions: 136107607 Number of successful extensions: 332572 Number of sequences better than 1.0e-05: 1056 Number of HSP's gapped: 329654 Number of HSP's successfully gapped: 1059 Length of query: 594 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 452 Effective length of database: 1,722,912,073 Effective search space: 778756256996 Effective search space used: 778756256996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)