BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0006.p (465 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D823.1 B1D823_9BACL Major facilitator superfamily MFS_1 204 1e-50 A6CMR7.1 A6CMR7_9BACI Tetracycline resistence protein TETA(L)/TETK 199 4e-49 B7JCW8.1 B7JCW8_BACC0 Major facilitator family transporter 134 3e-29 Q6HMC4.1 Q6HMC4_BACHK Major facilitator family transporter; poss... 133 4e-29 C3FZC9.1 C3FZC9_BACTU Major facilitator family transporter 133 4e-29 >B1D823.1 B1D823_9BACL Major facilitator superfamily MFS_1 Length = 446 Score = 204 bits (520), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 128/400 (32%), Positives = 199/400 (49%), Gaps = 2/400 (0%) Query: 37 LVIMNTAMFNLALPDVTETFGISASAASWIVTGYSIMFSIASITYSRLSDFLPXXXXXXX 96 +V+MNT MFN+++P + GI++ SWIV+ YSI ++++++ YSRLSD P Sbjct: 16 VVVMNTTMFNVSVPSIINDLGITSDLGSWIVSSYSIGYALSTVIYSRLSDIAPIRRLVAV 75 Query: 97 XXXXXXXXXVAGFFSTNFIVLLIVRIVQASGAGAVMSLSLVLFTRYVPLGRRGKAMATIM 156 V G F+ NF +LL RI+Q++GAG + L LVL +RY+P+ RRG+A++ I Sbjct: 76 GLLILGLSSVLGIFAHNFALLLTARILQSAGAGTMAGLGLVLASRYIPVERRGRAISMIA 135 Query: 157 XXXXXXXXXXXXXXXXITQYFGWNWLFAVTAIIXXXXXXXXXXXXXXXXSRGSFDAXXXX 216 I++YFGWN LF VT ++ SFD Sbjct: 136 AGSAMAFGLGPIVGGVISEYFGWNGLFVVTCLVLVILPVLLKLLPKENPQPASFDYWGAA 195 Query: 217 XXXXXXXXXXXXXXXXXWIALVAGIAAIFLFVSRIRTI--SDPFVMPALFKNRPYLVLSL 274 I L+A + I + V IR + FV P +F+ Y+ L + Sbjct: 196 WTVINATSLLIAVTQQSVIWLLASVITIVIHVLHIRKAQGNGTFVNPEVFRQPGYIRLLV 255 Query: 275 VGIASYLCSFATLFLLPQILVHRFGFTASHAGFVIFPGSLLAIFVSRSVGRIIDRFGNGG 334 VG + + LFL+P +L + F + GF+I PG++L+ ++ VGR +DR+GN Sbjct: 256 VGFCLLIINLGNLFLMPLVLANLFHKSEMLIGFLIAPGAILSAVLTPFVGRWLDRYGNVR 315 Query: 335 ILRXXXXXXXXXXXXXXXXXGHSWIAVMFVYMIMSLAFTTLSSSVSNEISRVLPSSQIGS 394 L G S +M Y++ S AF+ +S++N+ S +LP IGS Sbjct: 316 FLFIGTAVLTVVMALFMTALGSSPFVIMCGYLLFSPAFSATLASLNNQTSSILPRHLIGS 375 Query: 395 GMGLFQLLQFFSGAFGVAMAASALEWQHGLSLSAAYSNIY 434 GMG+ QL+QFF G+ VA+ L ++L AY+++Y Sbjct: 376 GMGMMQLIQFFGGSLSVAICGVLLHKMSSITLEHAYNHVY 415 >A6CMR7.1 A6CMR7_9BACI Tetracycline resistence protein TETA(L)/TETK Length = 444 Score = 199 bits (507), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 145/425 (34%), Positives = 209/425 (49%), Gaps = 2/425 (0%) Query: 30 LFSVAVVLVIMNTAMFNLALPDVTETFGISASAASWIVTGYSIMFSIASITYSRLSDFLP 89 + S+A+ LV+MNT MFN+ALP++ F ISAS +W+V+GYSI+ +I +ITY+RLSD+LP Sbjct: 18 VLSLAIWLVVMNTTMFNVALPNILNDFSISASEGAWVVSGYSIVLAICTITYTRLSDYLP 77 Query: 90 XXXXXXXXXXXXXXXXVAGFFSTNFIVLLIVRIVQASGAGAVMSLSLVLFTRYVPLGRRG 149 + GFF+ ++ +LL+ R++QA+GA A+ LS+V +RY+PL RG Sbjct: 78 IRRLIAIGISLFGLSSIIGFFAESYSLLLLARLMQATGAAAIPGLSMVFASRYIPLSTRG 137 Query: 150 KAMATIMXXXXXXXXXXXXXXXXITQYFGWNWLFAVTAIIXXXXXXXXXXXXXXXXSRGS 209 +AMA I IT+Y WN+LF VT ++ ++G Sbjct: 138 RAMAMIASASSLGFGLGPVVGGMITEYLAWNYLFIVTLMVIALIPVLLRILPKEEVAKGK 197 Query: 210 FDAXXXXXXXXXXXXXXXXXXXXXWIALVAGIAAIFLFVSRIRTISDPFVMPALFKNRPY 269 FD W L+AGI + L RI + PF+ P L K++ Y Sbjct: 198 FDFIGAVLTGLSVTAFLLFLSTFNWPFLLAGILLLGLLWLRINRSALPFIQPDLIKDKRY 257 Query: 270 LVLSLVGIASYLCSFATLFLLPQILVHRFGFTASHAGFVIFPGSLLAIFVSRSVGRIIDR 329 +L + +L FA L L+P +L F S GF+IFPG++ + + VGR+IDR Sbjct: 258 RLLLCISFLGFLTHFAILLLMPLMLGSIFSKAPSSIGFIIFPGAMFSAVAAIYVGRLIDR 317 Query: 330 FGNGGILRXXXXXXXXXXXXXXXXXGHSWIAVMFVYMIMSLAFTTLSSSVSNEISRVLPS 389 +GN +L S +M YM S F++LSSS +NE SR+L Sbjct: 318 YGNITVLFGAHVLLLISTLIFYFLSPLSEYWIMAGYMFTSFGFSSLSSSSTNETSRILSK 377 Query: 390 SQIGSGMGLFQLLQFFSGAFGVAMAASALEWQHGLSLSAA-YSNIYWGLXXXXXXXXXXX 448 +GSG+G+ QL F A G + A LEW HG S + Y L Sbjct: 378 EYVGSGIGMKQLTHFVGSASGSVVGAILLEW-HGQGFSTGDFQRSYLALIVAMGISLVLL 436 Query: 449 FAYRR 453 F Y R Sbjct: 437 FIYNR 441 >B7JCW8.1 B7JCW8_BACC0 Major facilitator family transporter Length = 463 Score = 134 bits (336), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 93/387 (24%), Positives = 170/387 (43%) Query: 33 VAVVLVIMNTAMFNLALPDVTETFGISASAASWIVTGYSIMFSIASITYSRLSDFLPXXX 92 V ++ +MN MFN+ALP++ + F + S SWI+T Y +++++ SI +L+D Sbjct: 18 VTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKD 77 Query: 93 XXXXXXXXXXXXXVAGFFSTNFIVLLIVRIVQASGAGAVMSLSLVLFTRYVPLGRRGKAM 152 + G +T + V+++ R++QA+GA + + ++++ RY +RG+A+ Sbjct: 78 LLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRAL 137 Query: 153 ATIMXXXXXXXXXXXXXXXXITQYFGWNWLFAVTAIIXXXXXXXXXXXXXXXXSRGSFDA 212 T IT + W +F ++ + GS D Sbjct: 138 GTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDI 197 Query: 213 XXXXXXXXXXXXXXXXXXXXXWIALVAGIAAIFLFVSRIRTISDPFVMPALFKNRPYLVL 272 + ++G A + F+ RIR +PF+ P LFKN+ + + Sbjct: 198 LGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAKEPFIKPILFKNKNFSIG 257 Query: 273 SLVGIASYLCSFATLFLLPQILVHRFGFTASHAGFVIFPGSLLAIFVSRSVGRIIDRFGN 332 L+ + SF+ F+ PQ L G T S+ GFV+ P ++ + + R GR+ D GN Sbjct: 258 LLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGN 317 Query: 333 GGILRXXXXXXXXXXXXXXXXXGHSWIAVMFVYMIMSLAFTTLSSSVSNEISRVLPSSQI 392 ++ G S + + + ++ T + S+SN IS+ L + Sbjct: 318 FALVFIASVFIFLAFSLLSTFIGISAFVIALILIFGNVGQTLMQISMSNTISQTLSKEET 377 Query: 393 GSGMGLFQLLQFFSGAFGVAMAASALE 419 G GMGL ++ F SGA +++ L+ Sbjct: 378 GVGMGLLSMINFISGAMAMSVVGKLLD 404 >Q6HMC4.1 Q6HMC4_BACHK Major facilitator family transporter; possible metal-tetracycline/H+ antiporter Length = 463 Score = 133 bits (335), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 93/387 (24%), Positives = 170/387 (43%) Query: 33 VAVVLVIMNTAMFNLALPDVTETFGISASAASWIVTGYSIMFSIASITYSRLSDFLPXXX 92 V ++ +MN MFN+ALP++ + F + S SWI+T Y +++++ SI +L+D Sbjct: 18 VTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKD 77 Query: 93 XXXXXXXXXXXXXVAGFFSTNFIVLLIVRIVQASGAGAVMSLSLVLFTRYVPLGRRGKAM 152 + G +T + V+++ R++QA+GA + + ++++ RY +RG+A+ Sbjct: 78 LLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRAL 137 Query: 153 ATIMXXXXXXXXXXXXXXXXITQYFGWNWLFAVTAIIXXXXXXXXXXXXXXXXSRGSFDA 212 T IT + W +F ++ + GS D Sbjct: 138 GTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDI 197 Query: 213 XXXXXXXXXXXXXXXXXXXXXWIALVAGIAAIFLFVSRIRTISDPFVMPALFKNRPYLVL 272 + ++G A + F+ RIR +PF+ P LFKN+ + + Sbjct: 198 LGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAKEPFIKPILFKNKNFSIG 257 Query: 273 SLVGIASYLCSFATLFLLPQILVHRFGFTASHAGFVIFPGSLLAIFVSRSVGRIIDRFGN 332 L+ + SF+ F+ PQ L G T S+ GFV+ P ++ + + R GR+ D GN Sbjct: 258 LLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGN 317 Query: 333 GGILRXXXXXXXXXXXXXXXXXGHSWIAVMFVYMIMSLAFTTLSSSVSNEISRVLPSSQI 392 ++ G S + + + ++ T + S+SN IS+ L + Sbjct: 318 FALVFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFMQISMSNTISQTLSKEET 377 Query: 393 GSGMGLFQLLQFFSGAFGVAMAASALE 419 G GMGL ++ F SGA +++ L+ Sbjct: 378 GVGMGLLSMINFISGAMAMSVVGKLLD 404 >C3FZC9.1 C3FZC9_BACTU Major facilitator family transporter Length = 463 Score = 133 bits (334), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 93/387 (24%), Positives = 170/387 (43%) Query: 33 VAVVLVIMNTAMFNLALPDVTETFGISASAASWIVTGYSIMFSIASITYSRLSDFLPXXX 92 V ++ +MN MFN+ALP++ + F + S SWI+T Y +++++ SI +L+D Sbjct: 18 VTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKD 77 Query: 93 XXXXXXXXXXXXXVAGFFSTNFIVLLIVRIVQASGAGAVMSLSLVLFTRYVPLGRRGKAM 152 + G +T + V+++ R++QA+GA + + ++++ RY +RG+A+ Sbjct: 78 LLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRAL 137 Query: 153 ATIMXXXXXXXXXXXXXXXXITQYFGWNWLFAVTAIIXXXXXXXXXXXXXXXXSRGSFDA 212 T IT + W +F ++ + GS D Sbjct: 138 GTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDI 197 Query: 213 XXXXXXXXXXXXXXXXXXXXXWIALVAGIAAIFLFVSRIRTISDPFVMPALFKNRPYLVL 272 + ++G A + F+ RIR +PF+ P LFKN+ + + Sbjct: 198 LGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAKEPFIKPILFKNKNFSIG 257 Query: 273 SLVGIASYLCSFATLFLLPQILVHRFGFTASHAGFVIFPGSLLAIFVSRSVGRIIDRFGN 332 L+ + SF+ F+ PQ L G T S+ GFV+ P ++ + + R GR+ D GN Sbjct: 258 LLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGN 317 Query: 333 GGILRXXXXXXXXXXXXXXXXXGHSWIAVMFVYMIMSLAFTTLSSSVSNEISRVLPSSQI 392 ++ G S + + + ++ T + S+SN IS+ L + Sbjct: 318 FALVFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFMQISMSNTISQTLSKEET 377 Query: 393 GSGMGLFQLLQFFSGAFGVAMAASALE 419 G GMGL ++ F SGA +++ L+ Sbjct: 378 GVGMGLLSMINFISGAMAMSVVGKLLD 404 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.139 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,789,823,375 Number of extensions: 55466038 Number of successful extensions: 214834 Number of sequences better than 1.0e-05: 946 Number of HSP's gapped: 217848 Number of HSP's successfully gapped: 1040 Length of query: 465 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 326 Effective length of database: 1,751,175,118 Effective search space: 570883088468 Effective search space used: 570883088468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 133 (55.8 bits)