BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0020.p (204 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2A103.1 B2A103_NATTJ Methyltransferase type 11 132 2e-29 B2KB51.1 B2KB51_ELUMP Methyltransferase type 11 94 5e-18 A7AZ37.1 A7AZ37_RUMGN Putative uncharacterized protein 92 4e-17 C3L081.1 C3L081_CLOB6 Putative methlytransferase 85 4e-15 B1QG60.1 B1QG60_CLOBO Methlytransferase, homolog 85 4e-15 >B2A103.1 B2A103_NATTJ Methyltransferase type 11 Length = 195 Score = 132 bits (332), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 64/187 (34%), Positives = 105/187 (56%), Gaps = 4/187 (2%) Query: 18 FNSLASDWSN--SDEAIRQAEKAVNVLGIQSGQSLLDVASGTGVILHALKQLQISPGHYL 75 FN A W N + E I+ A+ ++ I + ++LDV +GTG+I + ++ Y+ Sbjct: 10 FNDQAKVWDNKITQEHIKVAKDFISEYNIGANNAVLDVGAGTGIIYTIIDNKNLTD--YV 67 Query: 76 AMDISSAMLEKLHSAFPEAETACADFEQPFTCQRGFDYVLIYNSIPHFSDLDMLFTNAKR 135 +DI+ M+++ PEA+ C DFE ++ FD+V+I++ IPHF +L LF NA Sbjct: 68 GIDIAENMVKEFRKKHPEADIRCDDFELECQLEKAFDFVIIFDGIPHFRNLSKLFENAYN 127 Query: 136 SLLPGGTFMIAHSRTRQGLKEHHQRIGYKSERAPIPADEELALLAENYDFDQISFADEEF 195 +L G F+I HS+TR+ K + ++ GY SE++ IP D EL ++ F I D+ + Sbjct: 128 NLNKNGKFIIVHSKTRKEFKANQEKNGYNSEKSLIPTDAELIYHSKEKSFTNIIVEDDRY 187 Query: 196 FCFTCHR 202 F F C + Sbjct: 188 FKFVCEK 194 >B2KB51.1 B2KB51_ELUMP Methyltransferase type 11 Length = 195 Score = 94.4 bits (233), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 55/173 (31%), Positives = 90/173 (52%), Gaps = 5/173 (2%) Query: 18 FNSLASDWSNSD--EAIRQAEKAVNVLGIQSGQSLLDVASGTGVILHALKQLQISPGHYL 75 F+S+A W + E + E+ + I +LD+ GTGV+ L + +PG L Sbjct: 10 FDSIACGWDAKERSEVYAKIEQILAKCEITQKAKILDIGCGTGVLFPFLSKY--NPGEIL 67 Query: 76 AMDISSAMLEKLHSAFPEAETACADFEQPFTCQRGFDYVLIYNSIPHFSDLDMLFTNAKR 135 ++D+SS MLE+ PEA ADFE + +D ++ YN PHF + + +F NA + Sbjct: 68 SIDLSSKMLEEFKRKHPEANALLADFEDIKLEEDYYDNIIAYNVFPHFVNKEAVFYNAFK 127 Query: 136 SLLPGGTFMIAHSRTRQGLKEHHQRIGYKSERAPIPADEELALLAENYDFDQI 188 L GG F++AHS TR+ L H R ++ + +P+ E++ + F Q+ Sbjct: 128 FLKHGGIFVVAHSMTREELNSMHGR-KKETSKDLLPSSEQMKYFYASAGFKQV 179 >A7AZ37.1 A7AZ37_RUMGN Putative uncharacterized protein Length = 190 Score = 91.7 bits (226), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 61/190 (32%), Positives = 94/190 (49%), Gaps = 17/190 (8%) Query: 18 FNSLASDWSNSDEAIRQAEKAVNVLG-IQSGQSLLDVASGTGVILHALKQLQISPGHYLA 76 FN A+ W + ++++ Q + + L IQ G S+LDVA GTG + AL++ + P H A Sbjct: 7 FNQRAAHWDDGEQSVDQIRRMIAFLSDIQEGMSVLDVACGTGAMFEALRERK--PSHITA 64 Query: 77 MDISSAMLE------KLHSAFPEAETACAD-FEQPFTCQRGFDYVLIYNSIPHFSDLDML 129 +D+S M+E + S F E C D FE Q FD ++IYN+ PHF + D + Sbjct: 65 VDLSEKMIEIAARKVEGDSLF---ELQCRDLFEMT---QETFDRIIIYNAYPHFMEKDKV 118 Query: 130 FTNAKRSLLPGGTFMIAHSRTRQGLKEHHQRIGYKSERAPIPADEELALLAENYDFDQIS 189 L PGG F++AH R+ + H + + + A EE L + D I Sbjct: 119 VQKVAELLNPGGRFIVAHGACREKINGCHTNVPKEITSGLLSAKEESRLWETCFSID-IL 177 Query: 190 FADEEFFCFT 199 E+F+ F+ Sbjct: 178 IDTEQFYMFS 187 >C3L081.1 C3L081_CLOB6 Putative methlytransferase Length = 196 Score = 85.1 bits (209), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 54/184 (29%), Positives = 93/184 (50%), Gaps = 8/184 (4%) Query: 16 ERFNSLASDWSNSDEAIRQAEKAVNVL---GIQSGQSLLDVASGTGVILHALKQLQISPG 72 E FN+LA W D EK +L ++ G +LD+ SGTGV++ L+ + + G Sbjct: 5 EFFNNLADKWD--DMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNG 62 Query: 73 HYLAMDISSAMLE--KLHSAFPEAETACADFEQPFTCQRGFDYVLIYNSIPHFSDLDMLF 130 + A+DI+ ML+ K + + + DF + + ++ F+ ++ Y+ PHF D D L Sbjct: 63 NIAAIDIAENMLKVSKEKNKYSNLKFIVGDFLE-YKSKKTFNCIIAYSCYPHFKDKDKLA 121 Query: 131 TNAKRSLLPGGTFMIAHSRTRQGLKEHHQRIGYKSERAPIPADEELALLAENYDFDQISF 190 A L GG +IAHS +++ + H+ + +K + +P + A L E ++F I Sbjct: 122 HRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHILPKVKYTAELMEKHNFKTIYT 181 Query: 191 ADEE 194 D E Sbjct: 182 EDNE 185 >B1QG60.1 B1QG60_CLOBO Methlytransferase, homolog Length = 196 Score = 85.1 bits (209), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 54/184 (29%), Positives = 93/184 (50%), Gaps = 8/184 (4%) Query: 16 ERFNSLASDWSNSDEAIRQAEKAVNVL---GIQSGQSLLDVASGTGVILHALKQLQISPG 72 E FN+LA W D EK +L ++ G +LD+ SGTGV++ L+ + + G Sbjct: 5 EFFNNLADKWD--DMCFHSKEKIEYILSKANLKEGDRVLDIGSGTGVLIPYLENIISNNG 62 Query: 73 HYLAMDISSAMLE--KLHSAFPEAETACADFEQPFTCQRGFDYVLIYNSIPHFSDLDMLF 130 + A+DI+ ML+ K + + + DF + + ++ F+ ++ Y+ PHF D D L Sbjct: 63 NIAAIDIAENMLKVSKEKNKYSNLKFIVGDFLE-YKSKKTFNCIIAYSCYPHFKDKDKLA 121 Query: 131 TNAKRSLLPGGTFMIAHSRTRQGLKEHHQRIGYKSERAPIPADEELALLAENYDFDQISF 190 A L GG +IAHS +++ + H+ + +K + +P + A L E ++F I Sbjct: 122 HRAYELLEEGGRLVIAHSESKEKINSRHKDVDHKIKSHILPKVKYTAELMEKHNFKTIYT 181 Query: 191 ADEE 194 D E Sbjct: 182 EDNE 185 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,184,763,428 Number of extensions: 46849569 Number of successful extensions: 121673 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 121865 Number of HSP's successfully gapped: 34 Length of query: 204 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 75 Effective length of database: 1,845,385,268 Effective search space: 138403895100 Effective search space used: 138403895100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)