BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0010.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9K5S8.1 Q9K5S8_BACHD BH4010 protein 132 2e-29 Q5WB25.1 Q5WB25_BACSK Sortase 129 1e-28 Q5KYE8.1 Q5KYE8_GEOKA Hypothetical conserved protein 129 2e-28 C3J6A9.1 C3J6A9_9BACI Sortase family protein 129 2e-28 B7YC38.1 B7YC38_9BACI Sortase family protein 129 2e-28 >Q9K5S8.1 Q9K5S8_BACHD BH4010 protein Length = 207 Score = 132 bits (332), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 73/160 (45%), Positives = 99/160 (61%), Gaps = 5/160 (3%) Query: 57 EEEAPLP---DGMATPLEGTPPSKPAYTIGDIIGEIYLPRLDKRIAIVEGTERLQLKKGA 113 EE P+P D P++ P KP GD++GE+Y+P+L+ + I GT+ +L++G Sbjct: 42 EEPQPVPPAEDEPTEPVDSPYPLKPEK--GDLMGELYIPKLEATLPIYHGTDEDELERGV 99 Query: 114 GHDPGSAPIGAAGNSVLAGHRDTVFRGLGALQKNDLIEVETAEGRFTYSVTGSTIVDGDA 173 GH S G NSVL+GHRDTVFR LG + DL+ VETA G TY V IVD D Sbjct: 100 GHFANSVLPGEEDNSVLSGHRDTVFRELGEVGVGDLLIVETATGTHTYRVRKVRIVDEDD 159 Query: 174 RGVIKQSDEAVLTLITCYPFTYVGSAPDRYLLSAVLLRKE 213 R VI A LT+ TCYPF ++GSAP+RY+L A L++++ Sbjct: 160 RTVIVPKPRATLTVSTCYPFDFIGSAPERYILEATLIKEK 199 >Q5WB25.1 Q5WB25_BACSK Sortase Length = 227 Score = 129 bits (325), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 76/180 (42%), Positives = 106/180 (58%), Gaps = 18/180 (10%) Query: 55 HTEEEAPLPDGMATPLEGTPPSKPAYT------------IGDIIGEIYLPRLDKRIAIVE 102 H+E E +A P+E P + PAY IG+ +G + +P++D + I+ Sbjct: 52 HSETET-----VAAPVEEAPEAIPAYENDLQDVASWQPEIGEEMGTLTIPKIDATLPIIH 106 Query: 103 GTERLQLKKGAGHDPGSAPIGAAGNSVLAGHRDTVFRGLGALQKNDLIEVETAEGRFTYS 162 GT +L KG GH S G NSVL+GHRDTVFR LG + + D + VET G +TY Sbjct: 107 GTNEDELDKGVGHFADSVLPGEKDNSVLSGHRDTVFRKLGEVGEGDELIVETRSGVYTYK 166 Query: 163 VTGSTIVDGDARGVIKQSDEAVLTLITCYPFTYVGSAPDRYLLSAVLLRKEPV-SLSLQP 221 V IVD D R VI ++EA LT+ITCYPF ++G+APDRY+L A L+ ++ +L+L P Sbjct: 167 VHTVRIVDEDDRTVIVPTEEAQLTVITCYPFDFIGAAPDRYVLVADLIDEDVYETLALFP 226 >Q5KYE8.1 Q5KYE8_GEOKA Hypothetical conserved protein Length = 197 Score = 129 bits (323), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 70/145 (48%), Positives = 91/145 (62%), Gaps = 1/145 (0%) Query: 67 ATPLEGTPPSKPAY-TIGDIIGEIYLPRLDKRIAIVEGTERLQLKKGAGHDPGSAPIGAA 125 A P++ P P IG+ IGE+ +P+L+ +AI GT+ +L+KG GH GS G A Sbjct: 49 AAPIQIQAPLYPKLPKIGEQIGELIIPKLNMSLAIYHGTDENELEKGVGHYAGSVLPGEA 108 Query: 126 GNSVLAGHRDTVFRGLGALQKNDLIEVETAEGRFTYSVTGSTIVDGDARGVIKQSDEAVL 185 N VL+GHRDTVFR LG + + D++ V T+ G FTY V IVD D R VI +A L Sbjct: 109 DNCVLSGHRDTVFRRLGEVGQGDILIVRTSAGTFTYKVRKVRIVDADDRTVIVPKPKATL 168 Query: 186 TLITCYPFTYVGSAPDRYLLSAVLL 210 T+ TCYPF YVG AP RY+L A L+ Sbjct: 169 TVSTCYPFRYVGPAPQRYVLVANLI 193 >C3J6A9.1 C3J6A9_9BACI Sortase family protein Length = 197 Score = 129 bits (323), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 70/145 (48%), Positives = 91/145 (62%), Gaps = 1/145 (0%) Query: 67 ATPLEGTPPSKPAY-TIGDIIGEIYLPRLDKRIAIVEGTERLQLKKGAGHDPGSAPIGAA 125 A P++ P P IG+ IGE+ +P+L+ +AI GT+ +L+KG GH GS G A Sbjct: 49 AAPIQIQAPLYPKLPKIGEQIGELIIPKLNMSLAIYHGTDENELEKGVGHYAGSVLPGEA 108 Query: 126 GNSVLAGHRDTVFRGLGALQKNDLIEVETAEGRFTYSVTGSTIVDGDARGVIKQSDEAVL 185 N VL+GHRDTVFR LG + + D++ V T+ G FTY V IVD D R VI +A L Sbjct: 109 DNCVLSGHRDTVFRRLGEVGQGDILIVRTSAGTFTYKVRKVRIVDADDRTVIVPKPKATL 168 Query: 186 TLITCYPFTYVGSAPDRYLLSAVLL 210 T+ TCYPF YVG AP RY+L A L+ Sbjct: 169 TVSTCYPFRYVGPAPQRYVLVANLI 193 >B7YC38.1 B7YC38_9BACI Sortase family protein Length = 197 Score = 129 bits (323), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 70/145 (48%), Positives = 91/145 (62%), Gaps = 1/145 (0%) Query: 67 ATPLEGTPPSKPAY-TIGDIIGEIYLPRLDKRIAIVEGTERLQLKKGAGHDPGSAPIGAA 125 A P++ P P IG+ IGE+ +P+L+ +AI GT+ +L+KG GH GS G A Sbjct: 49 AAPIQIQAPLYPKLPKIGEQIGELIIPKLNMSLAIYHGTDENELEKGVGHYAGSVLPGEA 108 Query: 126 GNSVLAGHRDTVFRGLGALQKNDLIEVETAEGRFTYSVTGSTIVDGDARGVIKQSDEAVL 185 N VL+GHRDTVFR LG + + D++ V T+ G FTY V IVD D R VI +A L Sbjct: 109 DNCVLSGHRDTVFRRLGEVGQGDILIVRTSAGTFTYKVRKVRIVDADDRTVIVPKPKATL 168 Query: 186 TLITCYPFTYVGSAPDRYLLSAVLL 210 T+ TCYPF YVG AP RY+L A L+ Sbjct: 169 TVSTCYPFRYVGPAPQRYVLVANLI 193 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,370,053,582 Number of extensions: 60579361 Number of successful extensions: 207110 Number of sequences better than 1.0e-05: 479 Number of HSP's gapped: 207275 Number of HSP's successfully gapped: 479 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)