BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0009.p (529 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K743.1 B3K743_9BACI GerA spore germination protein 542 e-152 B1DHE4.1 B1DHE4_9BACL GerA spore germination protein 530 e-148 A7Z1B0.1 A7Z1B0_BACA2 GerKA 486 e-135 Q5KZH7.1 Q5KZH7_GEOKA Spore germination protein 481 e-134 B7YCV2.1 B7YCV2_9BACI GerA spore germination protein 480 e-133 >B3K743.1 B3K743_9BACI GerA spore germination protein Length = 532 Score = 542 bits (1397), Expect = e-152, Method: Compositional matrix adjust. Identities = 282/514 (54%), Positives = 377/514 (73%), Gaps = 7/514 (1%) Query: 12 KSDNPEQSAPAPFPLDPSLEVNLQELGQRIGHSSDIVVRRFTNESLGPLELALIYIDGLV 71 ++ +Q+A A L L+ NL + ++ GHS DI++R ++ LA+IYIDGLV Sbjct: 19 HNEQTKQTAEAS-RLSDRLDHNLHRIREQTGHSRDIIIRTLSHMFGSDHTLAVIYIDGLV 77 Query: 72 NADVVNQAVLQPLLETVKLNSELISA--ADAFHVIKNQMLPIGGVKEGNTLEALLPMLFD 129 + +N +++Q L + + EL ++ A+ +IKN+ L IG + + +E L+ + + Sbjct: 78 DTLTINHSIMQSL-TSKDYDEELHTSDPAEILSIIKNRFLSIGSISDLEHMEPLISAILE 136 Query: 130 GYTLILLGGLQAGMAADTSGWEKRGINEPTSQGVIRGPKEGFTESLRTGTSMLRRRLKTP 189 G T+IL+ G G+ A + G E+RG+ EP SQ V+RGPKEGFTE++RT T+++RR++K+P Sbjct: 137 GNTVILIDGSSHGVIASSIGGEERGVEEPQSQTVVRGPKEGFTENIRTNTALIRRKIKSP 196 Query: 190 DLRIEEYKIGQRTQTGVALVYLEGIASEQVLEEIRRRLKAINTDSILESNYIEEFIQDGG 249 +L I + KIG+ TQT VA++YL GIA++++++EI RL I+TDSILES YIEEFIQD Sbjct: 197 NLWIVDRKIGRVTQTDVAVMYLNGIANDKIVQEILNRLDRIDTDSILESGYIEEFIQDTT 256 Query: 250 LTPFPTIQNXXXXERPDAMAGGILEGQVGIIIDGTPFALLAPSTFFNFFQSSEDYYQRYD 309 TPFPTI N ERPDA+AG ILEG++ I++DGTPFAL+AP T F FQSSEDYYQ++D Sbjct: 257 FTPFPTITNT---ERPDAIAGAILEGKIAILVDGTPFALIAPITIFKLFQSSEDYYQKFD 313 Query: 310 ITSFLRLIRYSAFFVSMLLPALYIAVTTFHQEMLPTTLLISLAAQREGVXXXXXXXXXXX 369 I +FLRL+R +F VSMLLP+LYIA++TFHQEMLPTTLLISLAAQREG+ Sbjct: 314 IATFLRLLRIISFVVSMLLPSLYIAISTFHQEMLPTTLLISLAAQREGIPFPAVVEALAM 373 Query: 370 XXTFDVLREAGVRMPRTIGPAISIVGALVLGQAAVQAGLVSAAMVIVVSFTAISNFVIPS 429 TF+VLREAGVRMPR +G AISIVGALVLGQAAVQAGLVSAAMVI+V+FTAI++FV PS Sbjct: 374 EITFEVLREAGVRMPRAMGSAISIVGALVLGQAAVQAGLVSAAMVIIVAFTAIASFVAPS 433 Query: 430 LAIANSIRLIRFVMMFIAATLGLFGIMSFLIVLLIHMAGLRSFGVPYLSPVAPMVPRYLK 489 ++IANS RL+RF M +AATLGLFGIM+ L+ +LIH+AGLRSFG+PYL+P AP +P K Sbjct: 434 VSIANSARLLRFGFMILAATLGLFGIMAGLLAMLIHLAGLRSFGLPYLTPFAPFIPLDQK 493 Query: 490 DIFIRVPLWDMTMRPKTILGRETRRQGDKQKPKP 523 D+ +RVP W M RP I + +RQ Q+P P Sbjct: 494 DVLVRVPWWAMVTRPTLINRKNAKRQKMNQRPSP 527 >B1DHE4.1 B1DHE4_9BACL GerA spore germination protein Length = 529 Score = 530 bits (1365), Expect = e-148, Method: Compositional matrix adjust. Identities = 270/523 (51%), Positives = 365/523 (69%), Gaps = 14/523 (2%) Query: 8 ANRSKSDNPEQSAPAPFPLDPSLEVNLQELGQRIGHSSDIVVRRFTNESLGPLELALIYI 67 ++ ++ PEQ L P +++N++ + + +G DIV R F + S A++YI Sbjct: 15 SSTGQARQPEQV------LLPDMDINIERMHRFLGGGEDIVCRTFQHRSFDNTRYAILYI 68 Query: 68 DGLVNADVVNQAVLQPLLETVK--LNSELISAADAFHVIKNQMLPIGGVKEGNTLEALLP 125 DG+V +N +L L + + E + +H + + + IG + E +T+E Sbjct: 69 DGIVEGTTINTLLLSLLDDANNPYTDEEDGKQSSLYHHLTERSIAIGKLSEVSTIEQAGY 128 Query: 126 MLFDGYTLILLGGLQAGMAADTSGWEKRGINEPTSQGVIRGPKEGFTESLRTGTSMLRRR 185 + +G T++++ G + DT+G + R + EP+SQ VIRGPKEGFTE+LRT TS++RR Sbjct: 129 SILEGSTVLVVDGSLRAIVMDTTGGKTRAVEEPSSQTVIRGPKEGFTENLRTNTSLIRRI 188 Query: 186 LKTPDLRIEEYKIGQRTQTGVALVYLEGIASEQVLEEIRRRLKAINTDSILESNYIEEFI 245 +K+PD+R + IG++T+T VA+VY+EGIA E++++E+ RRL I+ D +LES Y+EEFI Sbjct: 189 IKSPDIRFDYKMIGRQTRTNVAVVYMEGIAEEEIVKEVHRRLDKIDIDGVLESGYVEEFI 248 Query: 246 QDGGLTPFPTIQNXXXXERPDAMAGGILEGQVGIIIDGTPFALLAPSTFFNFFQSSEDYY 305 QD TPFP +QN ERPDA + +LEGQV I++ GTPF L+AP TF FFQSSEDYY Sbjct: 249 QDRAFTPFPLLQNT---ERPDAASAALLEGQVLIMVSGTPFVLIAPVTFVKFFQSSEDYY 305 Query: 306 QRYDITSFLRLIRYSAFFVSMLLPALYIAVTTFHQEMLPTTLLISLAAQREGVXXXXXXX 365 QRYDI +FLR+IR ++FF+SMLLP+LYIA+TTFHQEMLPT+LLISLAAQREG+ Sbjct: 306 QRYDIATFLRIIRMASFFISMLLPSLYIAITTFHQEMLPTSLLISLAAQREGIPFPALLE 365 Query: 366 XXXXXXTFDVLREAGVRMPRTIGPAISIVGALVLGQAAVQAGLVSAAMVIVVSFTAISNF 425 TF++LREAGVRMPR IGPAISIVGALVLGQAAVQAG++SAAMVIVVSFTAI++F Sbjct: 366 AFLMEITFEILREAGVRMPRIIGPAISIVGALVLGQAAVQAGIISAAMVIVVSFTAIASF 425 Query: 426 VIPSLAIANSIRLIRFVMMFIAATLGLFGIMSFLIVLLIHMAGLRSFGVPYLSPVAPMVP 485 VIP++ + + RLIRF++M +A T GLFGIMS L+VLL H+ GLRSFG PY+ P +P VP Sbjct: 426 VIPAVNMGIAARLIRFILMILAGTFGLFGIMSGLMVLLAHLCGLRSFGKPYMMPFSPFVP 485 Query: 486 RYLKDIFIRVPLWDMTMRPKTILGRETR--RQGDKQKPKPQGE 526 LKD+ IRVP W M RP I G ++ R+G QKP P E Sbjct: 486 SNLKDVLIRVPWWAMRKRPINI-GSDSNAIREGAGQKPDPDQE 527 >A7Z1B0.1 A7Z1B0_BACA2 GerKA Length = 546 Score = 486 bits (1250), Expect = e-135, Method: Compositional matrix adjust. Identities = 244/518 (47%), Positives = 353/518 (68%), Gaps = 16/518 (3%) Query: 10 RSKSDN-PEQSAPAPFPLDPSLEVNLQELGQRIGHSSDIVVRRFTNESLGPLEL--ALIY 66 R ++D+ P++ P L SL+ N+++L +R G+S+D+V+RR +G EL A++Y Sbjct: 23 RKEADSQPDKERDEPISL--SLKQNMKKLSERFGNSADVVIRRL---KIGKEELPAAIVY 77 Query: 67 IDGLVNADVVNQAVLQPLLETVKLNSELISAADAFHVIKNQMLPIGGVKEGNTLEALLPM 126 I GLV+ +V+Q++L+ L++ + E A +I ++ L IGGVKE ++L Sbjct: 78 IAGLVDEQLVSQSLLEDLIDIPSVQPE-----QAVCLIYDKTLAIGGVKEAEKWKSLYAG 132 Query: 127 LFDGYTLILLGGLQAGMAADTSGWEKRGINEPTSQGVIRGPKEGFTESLRTGTSMLRRRL 186 L G TLI + G + A T G E R I EP++Q RGP++GFTESL+T SM+RR + Sbjct: 133 LVAGSTLIFVDGADKALIASTEGGEHRSIQEPSTQVSFRGPRQGFTESLQTNISMIRRYV 192 Query: 187 KTPDLRIEEYKIGQRTQTGVALVYLEGIASEQVLEEIRRRLKAINTDSILESNYIEEFIQ 246 KTPD+ +E+ +IG T T VAL+Y+ GI +++++E+R+RLK I+ DSILES YIE+ I+ Sbjct: 193 KTPDVWVEKMEIGHETNTDVALMYINGICDKKLIKEVRKRLKHIDIDSILESGYIEQLIE 252 Query: 247 DGGLTPFPTIQNXXXXERPDAMAGGILEGQVGIIIDGTPFALLAPSTFFNFFQSSEDYYQ 306 D + FPT+ + ERPD +AG +LEG+ II+DGTPF L+AP+ F FFQS EDYY Sbjct: 253 DAPFSVFPTVYHT---ERPDVVAGNLLEGRFAIIVDGTPFVLIAPALFIQFFQSVEDYYS 309 Query: 307 RYDITSFLRLIRYSAFFVSMLLPALYIAVTTFHQEMLPTTLLISLAAQREGVXXXXXXXX 366 R+DI + +R++R FF+S++ P +Y+A TTFHQEM+PT LLI +AAQRE V Sbjct: 310 RFDIATSIRVLRVLIFFISLVAPGIYVAATTFHQEMIPTQLLIVIAAQRETVPFPAVIEA 369 Query: 367 XXXXXTFDVLREAGVRMPRTIGPAISIVGALVLGQAAVQAGLVSAAMVIVVSFTAISNFV 426 F++LREAGVR+PR +G A+SIVGALV+GQAAVQAG+VS AMVIVV+ TAI++F Sbjct: 370 LTMEVAFEILREAGVRLPRVVGSAVSIVGALVIGQAAVQAGIVSPAMVIVVALTAIASFA 429 Query: 427 IPSLAIANSIRLIRFVMMFIAATLGLFGIMSFLIVLLIHMAGLRSFGVPYLSPVAPMVPR 486 P+ +A S RLIRF+ + AA +G +G++ +I++ +H+ GLRSFG PY+SP+AP+ P Sbjct: 430 TPAFDMAISARLIRFLFILSAAVMGFYGLILGIIMMFVHLCGLRSFGFPYMSPLAPLTPG 489 Query: 487 YLKDIFIRVPLWDMTMRPKTILGRETRRQGDKQKPKPQ 524 LKD +R PLW RP ++ RRQG+ Q+PKP+ Sbjct: 490 GLKDSLLRAPLWADEKRPGMLVQENNRRQGEHQRPKPE 527 >Q5KZH7.1 Q5KZH7_GEOKA Spore germination protein Length = 539 Score = 481 bits (1239), Expect = e-134, Method: Compositional matrix adjust. Identities = 245/519 (47%), Positives = 348/519 (67%), Gaps = 13/519 (2%) Query: 6 PRANRSKSDNPEQSAPAPFPLDPSLEVNLQELGQRIGHSSDIVVRRFTNESLGPLELALI 65 P + SD P++ PL L VN+ + Q+ G S+D+V+RRF ++ A+I Sbjct: 21 PDHSGEASDVPQE------PLSAELAVNIDIIRQKTGESNDVVIRRFALGQEWQIKAAII 74 Query: 66 YIDGLVNADVVNQAVLQPLLE-TVKLNSELISAADAFHVIKNQMLPIGGVKEGNTLEALL 124 ++DGLV+ V + VL PLL+ + L E + F I+ ++ +GGVK + L Sbjct: 75 FVDGLVDEKNVYEFVLTPLLQASFPLALE---EKERFQWIEKKLAAVGGVKHISDWRKLF 131 Query: 125 PMLFDGYTLILLGGLQAGMAADTSGWEKRGINEPTSQGVIRGPKEGFTESLRTGTSMLRR 184 LF G T++L+ G+ ++A T G E R I EP +Q +RGP+EGFTESLRT T+M+RR Sbjct: 132 FELFSGATVMLIDGVPFAISASTKGGEYRSIEEPQTQIAVRGPREGFTESLRTNTAMIRR 191 Query: 185 RLKTPDLRIEEYKIGQRTQTGVALVYLEGIASEQVLEEIRRRLKAINTDSILESNYIEEF 244 R++ P+L +E +IG TQT VA++Y++GIA++++++E+R RL+ I+ D +LES YIE+ Sbjct: 192 RIRNPNLWLETMQIGAVTQTDVAIMYIKGIANDEIVKEVRARLRRIDIDGVLESGYIEQL 251 Query: 245 IQDGGLTPFPTIQNXXXXERPDAMAGGILEGQVGIIIDGTPFALLAPSTFFNFFQSSEDY 304 I+D T FPT + ERPD +A +LEG+V + ++GTPF L+AP+ F FFQ+ EDY Sbjct: 252 IEDQTFTTFPTTYHT---ERPDIVAANLLEGRVAVFVEGTPFVLVAPALFIQFFQAVEDY 308 Query: 305 YQRYDITSFLRLIRYSAFFVSMLLPALYIAVTTFHQEMLPTTLLISLAAQREGVXXXXXX 364 Y R+DI++ LR +R FF+S++ PA+YIA TTFHQEM+PT L+I++AAQRE V Sbjct: 309 YARFDISTALRFLRVLIFFISLVAPAIYIAATTFHQEMIPTQLVIAIAAQREAVPFPGFV 368 Query: 365 XXXXXXXTFDVLREAGVRMPRTIGPAISIVGALVLGQAAVQAGLVSAAMVIVVSFTAISN 424 TF++LREAGVR+PR +G A+SIVGALV+GQAAV+AG VS+AMVIVVS TAI++ Sbjct: 369 EALVMEVTFEILREAGVRLPRAVGQAVSIVGALVIGQAAVEAGFVSSAMVIVVSITAIAS 428 Query: 425 FVIPSLAIANSIRLIRFVMMFIAATLGLFGIMSFLIVLLIHMAGLRSFGVPYLSPVAPMV 484 F PS AIA S RLIRF +MF+AA G +GIM ++V+ IH+ LRSFGVPY+SP+AP + Sbjct: 429 FATPSFAIAISARLIRFGLMFLAAMFGFYGIMMGILVMTIHLCSLRSFGVPYMSPLAPFI 488 Query: 485 PRYLKDIFIRVPLWDMTMRPKTILGRETRRQGDKQKPKP 523 P + D R+P W RP+ I + RQ QKP+P Sbjct: 489 PTNMGDTLFRLPTWMFRERPRLINQKNIVRQSGDQKPEP 527 >B7YCV2.1 B7YCV2_9BACI GerA spore germination protein Length = 541 Score = 480 bits (1236), Expect = e-133, Method: Compositional matrix adjust. Identities = 245/519 (47%), Positives = 346/519 (66%), Gaps = 13/519 (2%) Query: 6 PRANRSKSDNPEQSAPAPFPLDPSLEVNLQELGQRIGHSSDIVVRRFTNESLGPLELALI 65 P + SD P++ PL L VN+ + Q+ G S+DIV+RRF ++ A+I Sbjct: 23 PDHSGEASDVPQE------PLSAELAVNIDIIRQKTGESNDIVIRRFALGQEWQIKAAII 76 Query: 66 YIDGLVNADVVNQAVLQPLLE-TVKLNSELISAADAFHVIKNQMLPIGGVKEGNTLEALL 124 ++DGLV+ V + VL PLL+ + L E + F I+ ++ +GGVK + L Sbjct: 77 FVDGLVDEKNVYEFVLTPLLQASFPLALE---EKERFQWIEKKLAAVGGVKHISDWGKLF 133 Query: 125 PMLFDGYTLILLGGLQAGMAADTSGWEKRGINEPTSQGVIRGPKEGFTESLRTGTSMLRR 184 LF G T+IL+ G+ ++A T G + R + EP +Q +RGP+EGFTESLRT T+M+RR Sbjct: 134 FELFSGATVILIDGVSFAVSASTKGGQYRAVEEPQTQIAVRGPREGFTESLRTNTAMIRR 193 Query: 185 RLKTPDLRIEEYKIGQRTQTGVALVYLEGIASEQVLEEIRRRLKAINTDSILESNYIEEF 244 R++ P+L +E +IG TQT VA++Y++GI ++ +++E+R RL+ I+ D +LES YIE+ Sbjct: 194 RIRNPNLWLETMQIGTVTQTDVAIMYIKGIVNDGIVKEVRERLRRIDIDGVLESGYIEQL 253 Query: 245 IQDGGLTPFPTIQNXXXXERPDAMAGGILEGQVGIIIDGTPFALLAPSTFFNFFQSSEDY 304 I+D T FPT + ERPD +A +LEG+V + ++GTPF LLAP+ F FFQ+ EDY Sbjct: 254 IEDQTFTTFPTTYHT---ERPDIVAANLLEGRVAVFVEGTPFVLLAPAVFIQFFQAVEDY 310 Query: 305 YQRYDITSFLRLIRYSAFFVSMLLPALYIAVTTFHQEMLPTTLLISLAAQREGVXXXXXX 364 Y R+DI + LR +R FF+S++ PA+YIA TTFHQEM+PT L+I++AAQRE V Sbjct: 311 YARFDIATALRFLRVLIFFISLVAPAIYIASTTFHQEMIPTQLVIAIAAQREAVPFPGFV 370 Query: 365 XXXXXXXTFDVLREAGVRMPRTIGPAISIVGALVLGQAAVQAGLVSAAMVIVVSFTAISN 424 TF++LREAGVR+PR +G A+SIVGALV+GQAAV+AG VS+AMVIVVS TAI++ Sbjct: 371 EALVMEVTFEILREAGVRLPRAVGQAVSIVGALVIGQAAVEAGFVSSAMVIVVSITAIAS 430 Query: 425 FVIPSLAIANSIRLIRFVMMFIAATLGLFGIMSFLIVLLIHMAGLRSFGVPYLSPVAPMV 484 F PS AIA S RLIRF +MF+AA G +GIM ++V++IH+ LRSFGVPY+SP+AP + Sbjct: 431 FATPSFAIAISARLIRFGLMFLAAMFGFYGIMMGILVMMIHLCSLRSFGVPYMSPLAPFI 490 Query: 485 PRYLKDIFIRVPLWDMTMRPKTILGRETRRQGDKQKPKP 523 P + D R+P W RP+ I + RQ QKP+P Sbjct: 491 PTNMGDTLFRLPTWMFRERPRLINQKNIVRQSGDQKPEP 529 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,913,834,972 Number of extensions: 117039720 Number of successful extensions: 329866 Number of sequences better than 1.0e-05: 1093 Number of HSP's gapped: 327323 Number of HSP's successfully gapped: 1097 Length of query: 529 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 388 Effective length of database: 1,732,333,088 Effective search space: 672145238144 Effective search space used: 672145238144 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)