BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0008.p (367 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DHE5.1 B1DHE5_9BACL Spore germination protein 170 2e-40 A5D161.1 A5D161_PELTS Hypothetical membrane protein 137 2e-30 Q4V1W1.1 Q4V1W1_BACCZ Spore germination protein 119 4e-25 C5D3G1.1 C5D3G1_9BACI Spore germination protein 119 6e-25 C3HC12.1 C3HC12_BACTU Spore germination protein 114 1e-23 >B1DHE5.1 B1DHE5_9BACL Spore germination protein Length = 369 Score = 170 bits (431), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 113/371 (30%), Positives = 188/371 (50%), Gaps = 10/371 (2%) Query: 1 MNRKIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAV 60 M I T Q LI + I PT + +P+++ + A DA ++ +++ G+++A +G Sbjct: 1 MKPAISTLQTVALIQSAITPTLVLAIPSVMVTIAHHDAWISGIIAIAAGMIMALGIGLLC 60 Query: 61 RCTNGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXX 120 + G F+ W+ I ++GLF+ +Y ++ I+R F +F+ D VL +TP Sbjct: 61 KANKGQLFVEWMESSFGRPIGMVIGLFIGIYYFNSFCVIVRSFADFIADIVLDSTPLFML 120 Query: 121 XXXXXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLAD 180 + V QG+EAIAR L +++ +L+P+FD S A Sbjct: 121 TAIMVVVAAFTVMQGIEAIARSAFLVLILILLIVPVSVFVLYTDIDFKRLIPLFDTSTAR 180 Query: 181 LTLASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGPD 240 L +AS P +SEVAVLL + PY++ P A G G + ++ ++++L +FG Sbjct: 181 LAIASFPPIGLLSEVAVLLLINPYMKKPASAVKAGLLGTFLGSSQLVLIILLSLTIFGQQ 240 Query: 241 LVRLSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGS 300 LV +Y + IV +G F+E+++I +S WVL++YVK ++FLFA V C + ++ S Sbjct: 241 LVPFMSYAFVNVMAIVEVGEFLERIEIFTVSVWVLTMYVKLAVFLFAAVHCLFHSLRLRS 300 Query: 301 SRPFIGTLGLLIVLECLYTW----ESTEKLNLYNKEGRFLVFFIFNVLVP-LGAVGLDRF 355 R + L +L ++ W EST L N V I N+L+P L A+GL Sbjct: 301 ERHTVMGLSILAIVTAAGEWPQDMESTFMTTLVNMP----VLLITNILLPILIALGL-LV 355 Query: 356 TKAKAKRKGWG 366 T+ K K++ G Sbjct: 356 TRGKRKQQRGG 366 >A5D161.1 A5D161_PELTS Hypothetical membrane protein Length = 366 Score = 137 bits (345), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 88/310 (28%), Positives = 150/310 (48%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVRCT 63 KI + QA ML+V+ +L TA + +PA + +A QDA L+I+ S GLLIA +V S Sbjct: 6 KIDSNQAIMLLVSMVLSTAILTVPAFVIKFARQDAWLSIMTSILVGLLIAMLVVSLSLRF 65 Query: 64 NGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXX 123 G + E ++GL + ++L T+A I+ EF +FL ++ NTP Sbjct: 66 PGKTLFEYAEEILGKAPGKLIGLLYIWWFLHTNALIIDEFASFLCTAMMPNTPTIVFFIA 125 Query: 124 XXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADLTL 183 Y VR GLE ++R N +M +LLP+FD L + Sbjct: 126 VVAVAAYAVRNGLEVLSRYNQLFLPIVLGLLFIVLILTTKDMKAARLLPVFDIGLVPVLK 185 Query: 184 ASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGPDLVR 243 + P +W+ EV VL + P+L P +A + +F L++ + + +FGP Sbjct: 186 GATVPASWLGEVVVLAMIIPFLNKPNEAYRVAALATLFTGLVLTACTLAVVAIFGPVTSG 245 Query: 244 LSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGSSRP 303 +P ++A +V + F+E+L+ + ++ WV + K +F +A V Q + S +P Sbjct: 246 HFVFPVYNAVRVVSIANFLERLESVIVAVWVFGGFAKVGVFYYAAVLGSAQWLGLKSYQP 305 Query: 304 FIGTLGLLIV 313 + +G+++V Sbjct: 306 LVAPVGVILV 315 >Q4V1W1.1 Q4V1W1_BACCZ Spore germination protein Length = 365 Score = 119 bits (299), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 78/311 (25%), Positives = 145/311 (46%), Gaps = 2/311 (0%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVRCT 63 KI Q A++I+ TI+ TA +++PAI S YA +D ++ +L++ G +IV +C Sbjct: 6 KISALQMALMIIPTIIATAALIVPAISSKYAGRDVWISPVLASLNGFFTVFIVYQLHKCY 65 Query: 64 NGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXX 123 + + + I+G L F L RE+ +F+ + L TP Sbjct: 66 PKETIIQYSRHIVGQIPGKIVGFIYLFFVLHMCGVTTREYADFIIGSFLPKTPMIVVIGS 125 Query: 124 XXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADLTL 183 + VR G+E + RV + + + + + P+ +H + L Sbjct: 126 VIIVCAFAVRGGVEVLGRVAQLLVPLFLLPPLLFVLLIPD-LKIENMFPIMEHGMMPSIL 184 Query: 184 ASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGPDLVR 243 + P W+SEV+++ + P++ + R G +I V L++++ +I++++FG V Sbjct: 185 GAAVPQAWISEVSLISMLLPFVIDHEKGRKWGIISVIVVMLILVYINIISVLLFG-GTVN 243 Query: 244 LSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGSSRP 303 YP F+A + + F E L+ + I+ WVL ++K S+ +A V Q + RP Sbjct: 244 NHIYPVFTAFRYISVATFFEHLESIVITLWVLGAFIKISVLYYALVLGIAQWLHLSDYRP 303 Query: 304 FIGTLGLLIVL 314 + LG L++L Sbjct: 304 IVFPLGFLVLL 314 >C5D3G1.1 C5D3G1_9BACI Spore germination protein Length = 370 Score = 119 bits (297), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 82/340 (24%), Positives = 155/340 (45%), Gaps = 3/340 (0%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVRCT 63 KI Q M++ TI+ TA +++PAI + +A+QD L+ L ++ G LI +I + Sbjct: 6 KISAAQMGMMMYPTIIATAILLVPAITARHAKQDMWLSPLWASLIGFLIVHIAVQLHQLY 65 Query: 64 NGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXX 123 + + + + ++GL L F+L S I+RE+ F+ N L +TP Sbjct: 66 PEKTLIEYSRDILGKFLGKVVGLIFLFFHLHISGIIVREYGEFVVGNFLFHTPLFFIMGS 125 Query: 124 XXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADLTL 183 + VR G+E I R+ L +M + P+ + + + Sbjct: 126 MILVCSFAVRGGVEVIGRLAEMIVPIVIILIVVNIILLIPDMEAKNMFPIMEKGIMPSLM 185 Query: 184 ASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGPDLVR 243 ++ P W SE ++ F+ PY+ + + G + V L + F + L + G R Sbjct: 186 GAMVPQGWFSEFLLIAFLLPYVADRNKGKKWGMISVTAVMLTLSFINIAILFILGGITDR 245 Query: 244 LSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGSSRP 303 + YPG SA + + F+E L+ + ++ WV +++K ++F +A V Q + RP Sbjct: 246 FT-YPGMSAVRYISIADFLEHLEAVVMALWVSGVFLKIAVFYYALVLGTAQWLNLSDYRP 304 Query: 304 FIGTLGLLIVLECLYTWESTEKLNLYNKEGRFLVFFIFNV 343 + +GLL++L + W + + L + G F++ ++ Sbjct: 305 IVFPMGLLLLLFAI--WSAPNLMELIHFLGSSSPFYLMSI 342 >C3HC12.1 C3HC12_BACTU Spore germination protein Length = 367 Score = 114 bits (285), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 81/328 (24%), Positives = 155/328 (47%), Gaps = 6/328 (1%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVRCT 63 +I + Q A +IV TI+ TA +++PAI +A +D ++ L++ G +I+ R Sbjct: 5 RISSFQMACMIVPTIIATAILIVPAITGKFAGRDMWISSTLASVNGFFTVFILYRLHRIF 64 Query: 64 NGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXX 123 F+ + + ILG L F+L + +I RE+ +F+ + L TP Sbjct: 65 PNKTFVQYSECIIGRIPGKILGFAYLLFFLHVAGSICREYADFVIGSFLPKTPMIVVLGS 124 Query: 124 XXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADLTL 183 + +R G+E + R + + E+ V + P+ +H + + + Sbjct: 125 IVIICAFAIRGGVEVLGRA-AQLLVPLFILPLFLLILILPELKVVNIFPIMEHGVMPVIM 183 Query: 184 ASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWT--GLIFVALLMMFSLVIALMVFGPDL 241 + P W +EV ++ + PY+ A RQ + + W+ +I LL+ ++ +I++ FG + Sbjct: 184 GAAVPQGWFAEVFMISMLLPYV-ADRQ-KGMKWSIISVIVAMLLLTYTNIISIFHFGESV 241 Query: 242 VRLSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGSS 301 S YP FS + G F E L+ + I++W+L I+VK S+F +A Q + Sbjct: 242 TSYS-YPVFSVFRYISFGSFFEHLESVVITFWILGIFVKISVFYYALTLGIAQWLQCYEY 300 Query: 302 RPFIGTLGLLIVLECLYTWESTEKLNLY 329 RP + +G L+ L ++ S +++ + Sbjct: 301 RPIVFPVGFLLTLFGMWAASSEQEMTQF 328 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,836,379,957 Number of extensions: 69126803 Number of successful extensions: 255146 Number of sequences better than 1.0e-05: 326 Number of HSP's gapped: 255029 Number of HSP's successfully gapped: 326 Length of query: 367 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 230 Effective length of database: 1,770,017,148 Effective search space: 407103944040 Effective search space used: 407103944040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)