BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0007.p (410 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DHE6.1 B1DHE6_9BACL Germination protein, Ger(X)C family 258 1e-66 B3K744.1 B3K744_9BACI Germination protein, Ger(X)C family 211 1e-52 C0Z585.1 C0Z585_BREBN Putative spore germination protein 148 1e-33 C0Z4B2.1 C0Z4B2_BREBN Spore germination protein 144 1e-32 B1HQ82.1 B1HQ82_LYSSC Spore germination protein KC 138 9e-31 >B1DHE6.1 B1DHE6_9BACL Germination protein, Ger(X)C family Length = 410 Score = 258 bits (658), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 135/388 (34%), Positives = 218/388 (56%), Gaps = 3/388 (0%) Query: 23 GCWDDRELSELGITSGSAYDWEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 GCW+ EL+E+ ITS + DW+ + W +YQV+ P P + ++ Sbjct: 23 GCWNRIELNEVAITSATGIDWDGDSWIVSYQVVIPAAISSVMGGSGGSGPKSP-IIVYST 81 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVFVFI 142 KG+TI +A+ + S R++FF+H+R+ V+GE +HG+N+LID++ R RETV V Sbjct: 82 KGKTIRDAIMHSYSESPRKLFFAHNRVVVIGEKALQHGLNELIDVYFRNSSTRETVSVLA 141 Query: 143 AKGEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAVLPE 202 G A IL+Q MQL GAG++ +I E + S P +++LAM ++ ++ SAV+PE Sbjct: 142 TPGTARRILEQFMQLQAISGAGMKEIILSEMKNTSILPNIPVYQLAMEVTGDAKSAVVPE 201 Query: 203 ILLTGPEIMDETQETGRTDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIKTAT 262 I ++G E + G T + ++L LGRL VLK +K++GW+S+K++ G S++ D +K + Sbjct: 202 IFVSGTEDTSSLRSFGSTSIPSKLKLGRLAVLKDDKMIGWMSRKESLGASYLRDSVKQSY 261 Query: 263 IAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLNERA 322 + P + + G K + + KS T V PK + + + +G L E DL + Sbjct: 262 VVVPCEDDKGKKGYDTIHIFKSATKVIPKLKNNQLTFTAEVDVTGTLMESTCAQDLYKPG 321 Query: 323 SITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSWDRVFQ 382 I +EQ + QV L+ +WK+ ++L AD+ G A K HR K++K K+ WD VF+ Sbjct: 322 IIQSLEQGGQLQVSALLQEAWKKGQQLNADIFGAAEKTHRKYPKQWKAWSKN--WDEVFK 379 Query: 383 EIEIRPHVSIEIERTGLSNKSFQSVHEK 410 + + V + R GLSNKSF + +K Sbjct: 380 QAPLETKVKFRMARMGLSNKSFDYLMDK 407 >B3K744.1 B3K744_9BACI Germination protein, Ger(X)C family Length = 405 Score = 211 bits (537), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 120/389 (30%), Positives = 214/389 (55%), Gaps = 8/389 (2%) Query: 23 GCWDDRELSELGITSGSAYDWEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 GCWD +EL+ L I S ++ D +++W ++QV+ P P F+ Sbjct: 20 GCWDSKELNSLSIVSATSIDRSEDEWIISFQVVIPQSIATQTGGGSGGSQS--PITIFST 77 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARH-GVNQLIDLFLRKQDARETVFVF 141 +G+TI EA++ NL + R +FF+H+ I ++ E V R+ GV Q++D FLR ++RET+ V Sbjct: 78 RGKTIREAMQNANLEAPRALFFAHNSILIINEQVVRNAGVQQILDFFLRPMESRETMSVL 137 Query: 142 IAKGEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAVLP 201 + K +A ++L+ L+ L K G IQ +I Q LS ++ + A +++ SA+ P Sbjct: 138 LTKDKASNLLEVLIPLEKISGNAIQRVITQGQENLSQVKNIKLIDFASMVANPYQSAMAP 197 Query: 202 EILLTGPEIMDETQETGR-TDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIKT 260 E+ ++G + T + + T Q L LG LGV +G++LVGW+ ++++ G++++++ +K Sbjct: 198 ELRVSGNQEEQSTLDALKSTRNQAVLKLGDLGVFRGDRLVGWMKRRESRGVAWLSNSVKN 257 Query: 261 ATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLNE 320 I P + + +S+ + S T V PK I ++I+ G L E MDL + Sbjct: 258 LIIVTPCSDQDATRL-SSYRVTHSSTRVDPKMVNGSVNIMVHIQTKGALDETSCTMDLTK 316 Query: 321 RASITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSWDRV 380 I+++E++L +Q+ E V +WK ++ L DV GF IHR K++ K ++ Sbjct: 317 PEVISKLEEALAEQIKEEVLATWKTMKDLNVDVVGFLDMIHRRYPAASKRLSK---LEQP 373 Query: 381 FQEIEIRPHVSIEIERTGLSNKSFQSVHE 409 QE+E+ ++ + +E T + NK F ++ E Sbjct: 374 LQELELDLNIEVNVEHTNMINKPFSNLIE 402 >C0Z585.1 C0Z585_BREBN Putative spore germination protein Length = 393 Score = 148 bits (373), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 100/385 (25%), Positives = 178/385 (46%), Gaps = 13/385 (3%) Query: 23 GCWDDRELSELGITSGSAYDWEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 GCWD +EL++L I G D ++ + QV++P P FT+ Sbjct: 18 GCWDRKELNDLAIAVGIGIDKVQTGYQVSVQVVDPAEVAANKGKPGRA-----PVTLFTM 72 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVFVFI 142 TI EAV K S R+++ +H+R+ + GE VAR G+ +ID R + R ++ I Sbjct: 73 NAPTIFEAVRKMTTISPRKIYMAHTRMLIFGEEVAREGIAPVIDFVSRDHEFRTDFYIAI 132 Query: 143 AKG-EAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAVLP 201 A+G A +IL + L + + +E S++ + M +L +S+ AVL Sbjct: 133 ARGTTARNILRVITPLEQIPSTEMFASLETSSKVWAPTAAVTMDQLITDFTSKGKEAVLT 192 Query: 202 EILLTGPEIM-DETQETGRTDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIKT 260 + LTGP D+ + D L L +G+K++ WLS+ + S++T K+K+ Sbjct: 193 GVRLTGPAREGDKRNNVEQIDTPVLLQYTTLATFRGDKMINWLSEADSKAYSYITGKVKS 252 Query: 261 ATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLNE 320 + P K GG + ++ K ++ + ++ + E+ +DL E Sbjct: 253 TVGSMPCK--GGGLLTLEVMHADAKIEATMKNGTPEVIVHIALEND--IGEVQCKLDLTE 308 Query: 321 RASITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSWDRV 380 +I E+E+ E+ + L + +K G D+ GF IHR K +K+I +W++ Sbjct: 309 EDTIKELEKEAEKSLEALATKAIANAKKNGVDIYGFGEAIHRQYPKVWKKI--GSNWEKG 366 Query: 381 FQEIEIRPHVSIEIERTGLSNKSFQ 405 F ++ + ++I R G N +F+ Sbjct: 367 FSKLPVHVKADVKIRRLGTINNAFE 391 >C0Z4B2.1 C0Z4B2_BREBN Spore germination protein Length = 400 Score = 144 bits (364), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 96/387 (24%), Positives = 188/387 (48%), Gaps = 17/387 (4%) Query: 23 GCWDDRELSELGITSGSAYDWEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 GCW+ REL++L I G+ D D ++ + Q++NP T+++ Sbjct: 19 GCWNRRELNDLAIVMGTGIDKIDGEYVISTQIVNPGGVASKEGGGMNQSS----VATYSM 74 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVFVFI 142 + +I E + K + R ++FSH ++ V+GE +A+ G +++DL R + R +V + Sbjct: 75 RASSIFEGIRKMTTVTPRRLYFSHIQVLVLGEQIAKEGAKEVLDLLFRDHEVRPDFYVIL 134 Query: 143 AK-GEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAVLP 201 AK A IL + L K + +E ++ E L + VL Sbjct: 135 AKDATALQILSMIDPLEKIPATKLYKSLETAESAWGSIVSIQLDEFIADLLRDGIEPVLV 194 Query: 202 EILLTGPEIMDETQETGRTDLQTRLA-LGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIKT 260 + G M + + + Q+ L +GV KG+KL GW +++++ G S++TD++ + Sbjct: 195 GVNSKGKVHMGTSGGNSQKESQSSLLRYHEIGVFKGDKLRGWFNEEESIGFSYITDRVDS 254 Query: 261 ATIAFPSKPESGGKPDASFVLQ--KSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDL 318 P + V++ +S+TTV K EK ++++I G + E+ DL Sbjct: 255 TVEEIPCDKNN------KIVIEIIRSKTTVNGKVEKGKPQVEVSIFAEGNVGEVACSNDL 308 Query: 319 NERASITEMEQSLEQQVL-ELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSW 377 ++ +I ++E +E+Q+ ++V K ++ +DV GF I+++D K ++++++D W Sbjct: 309 SDPKTIYKLEADVEKQIRGKIVSAIHKAQKQYRSDVFGFGQSIYKNDPKGWEKVKED--W 366 Query: 378 DRVFQEIEIRPHVSIEIERTGLSNKSF 404 D F ++++ +++ RTG SF Sbjct: 367 DSQFPNLKVKIKTDVKLRRTGKVGNSF 393 >B1HQ82.1 B1HQ82_LYSSC Spore germination protein KC Length = 379 Score = 138 bits (348), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 95/380 (25%), Positives = 182/380 (47%), Gaps = 16/380 (4%) Query: 23 GCWDDRELSELGITSGSAYDWEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 GCW+ REL+EL I + D D + + Q++NP P VT+ Sbjct: 4 GCWNKRELNELAIVTAVGVDKTDEFIELSIQIVNPSQIATKNSTSFQV-----PVVTYHA 58 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVFVFI 142 KG+T+ EA+ K + R+ +++H+++ ++GE +A +G+N ++DLF R + R + + Sbjct: 59 KGKTLFEAIRKLTTQTPRKPYYAHTQVIIIGEEMAEYGMNSILDLFQRDPEGRSDFNIIV 118 Query: 143 A-KGEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAVLP 201 A + A DIL L L + + ++ L + +L +L S SAVLP Sbjct: 119 AHESTAQDILSILTPLEDIPASKLLKSLKDSEDALGTTESVILDDLINSLGSIDHSAVLP 178 Query: 202 EILLTG-PEIMDETQETGRTDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIKT 260 I + G + + + D L G L V K KL+G+LS++++ G +F+ + IK Sbjct: 179 SIQIHGDADAGKSSSNIEKMDSPANLQYGGLAVFKNYKLIGFLSEEESRGYNFLHNHIKG 238 Query: 261 ATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLNE 320 P + GK A S T V ++ D I + ++ + E+ +DL + Sbjct: 239 TVQKVPCSEK--GKISAEVF--NSSTKVTGSFKNDKPSIHVQVELEQNIAEISCPLDLTK 294 Query: 321 RASITEMEQSLEQQVLELVDNSWKEVRK-LGADVTGFAIKIHRSDRKRFKQIRKDKSWDR 379 +I + + + V ++ ++ ++K D+ F+ +HR D + +K+I+KD W Sbjct: 295 PETIDTLNKETSKLVQANLEQTFHTIQKTYQVDILKFSEVLHREDHQAWKRIKKD--WAT 352 Query: 380 VFQEIEIRPHVSIEIERTGL 399 ++ + + H+ ++++ G+ Sbjct: 353 LYPNLPV--HIEVKVKTKGI 370 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,045,994,783 Number of extensions: 75362294 Number of successful extensions: 208111 Number of sequences better than 1.0e-05: 313 Number of HSP's gapped: 207842 Number of HSP's successfully gapped: 315 Length of query: 410 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 272 Effective length of database: 1,760,596,133 Effective search space: 478882148176 Effective search space used: 478882148176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)