BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0005.p (365 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K745.1 B3K745_9BACI Spore germination protein 256 2e-66 B1DHE3.1 B1DHE3_9BACL Spore germination protein 206 3e-51 A9U728.1 A9U728_PHYPA Predicted protein 188 7e-46 Q97TS5.1 Q97TS5_CLOAB Spore germination protein, GRKB 160 2e-37 A7GUP4.1 A7GUP4_BACCN Spore germination protein 159 5e-37 >B3K745.1 B3K745_9BACI Spore germination protein Length = 363 Score = 256 bits (655), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 138/340 (40%), Positives = 212/340 (62%), Gaps = 1/340 (0%) Query: 5 EQVSALQLSFMIMLFEIGSTPLFLLGAKAKQDSWLAMCTGSAAGLVLLMLLMWIQYRSRH 64 E++ Q+ M +LF IGSTPL+ LG +AKQD+WL + AGL+LL + ++IQ ++ Sbjct: 2 EKIGKYQIIAMTILFMIGSTPLYELGIEAKQDAWLVVLVAMLAGLLLLCVYLYIQNKNSE 61 Query: 65 NDLIGMLALHFGKIAGSALGGMYSLYFAYQSMRNVRDLGELTALTMLPNSPMSVTMLIFV 124 + L +L HFGK GS + Y +YFAY+SMRN R+ ++ ++ LP++P+ + +L+ V Sbjct: 62 HALPQILNRHFGKYLGSLITAAYIVYFAYESMRNTREFADIINISFLPSTPLFILILLMV 121 Query: 125 GTALYAIWKGTEVVFRLPEVLLPVVLSFYAVLVLLLGIMGSLDFNHLAPVFENGFKPIAD 184 + YA+WKG EV FR+ E LLP + Y ++VL+ + + L P+ ENG +P+ Sbjct: 122 LLSGYAVWKGIEVFFRVTEFLLPFTMIGYILIVLMFIGSKIIHLDRLMPMLENGIQPVLQ 181 Query: 185 AALPDIVSFPFGQMIVFLMLWSLWDKPGVPVKSTVTAYLLISIFLIFMNALNIAVLGPSI 244 AALP+++SFPFGQ++VFLM WS D K+++++Y+ + IFL+ N LN+A+L PSI Sbjct: 182 AALPEVISFPFGQIVVFLMFWSHLDDKKTLSKTSLSSYIFVGIFLMIFNILNLAILDPSI 241 Query: 245 AGTSQLPFLKTVRTLSSLKFIERLDILVTIQLFIGLLIKMMLFYFCSVKALTGLTGKSAK 304 S P L++VR + F+ERLD L+ + ++IG+ +KM FY +V L+ L S K Sbjct: 242 TSISTFPLLRSVRLIEIADFLERLDPLIILLIYIGIFVKMTAFYLGAVLGLSSLIKISHK 301 Query: 305 WWVFPVGAVIYGASFLERDYTQHIAIGLGPSLKID-PVFQ 343 VGA I+ SF ++ HI IG +LK P+FQ Sbjct: 302 KTTMIVGAFIFTISFTSPNFIYHIWIGFAQNLKYHFPIFQ 341 >B1DHE3.1 B1DHE3_9BACL Spore germination protein Length = 377 Score = 206 bits (523), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 133/356 (37%), Positives = 210/356 (58%), Gaps = 5/356 (1%) Query: 7 VSALQLSFMIMLFEIGSTPLFLLGAKAKQDSWLAMCTGSAAGLVLLMLL-MWIQYRSRHN 65 V+ Q++ M++LF IGS+ LFLLG A++D+WL++ G AG ++ L+ + IQ Sbjct: 2 VTLRQMATMVILFLIGSSSLFLLGGTAERDAWLSVAVGVLAGFAVISLVTLQIQRLEPDR 61 Query: 66 DLIGMLALHFGKIAGSALGGMYSLYFAYQSMRNVRDLGELTALTMLPNSPMSVTMLIFVG 125 +LI + L+FGK+ G G +Y +YF Y+ RNVR+ +L+ + +L ++P+SV MLI Sbjct: 62 NLIEIFKLYFGKVIGFIFGLVYVVYFCYKCTRNVREFADLSIMFLLSDTPLSVIMLIICI 121 Query: 126 TALYAIWKGTEVVFRLPEVLLPVVLSFYAVLVLLLGIMGSLDFNHLAPVFENGFKPIADA 185 YA+ G V FR+ EVLLP+++ YA++ LLL I G++D L P+ E GFKP+ DA Sbjct: 122 IGGYAVMGGPGVFFRMAEVLLPILILIYALIYLLLIITGTIDLARLLPMLEKGFKPVWDA 181 Query: 186 ALPDIVSFPFGQMIVFLMLWSLWDKP--GVPVKSTVTAYLLISIFLIFMNALNIAVLGPS 243 A+P+++SFPFG+M++FLM + D G ++ T+ +YL F+ MN + A LG S Sbjct: 182 AIPELISFPFGEMVLFLMFYRYIDNKSFGQVIRVTLKSYLFAGAFITVMNVVITASLG-S 240 Query: 244 IAGTSQLPFLKTVRTLSSLKFIERLDILVTIQLFIGLLIKMMLFYFCSVKALTGLTGKSA 303 +A S +P L+ ++ ER D V + F + +K+ +Y + AL L S Sbjct: 241 LAAWSTVPLLQATTFVAIGDVFERFDPFVALLFFTAVYVKLTAYYLGASLALAYLLRISL 300 Query: 304 KWWVFPVGAVIYGASFLERDYTQHIAIGLGPSLKID-PVFQVAIPLLLAVSILLRS 358 + PVG I+ SF + Y + +G ++K P+FQ+ IP LL + +LLRS Sbjct: 301 RIAAIPVGIGIFFGSFWFKSYMHQVNVGFELNVKYHFPIFQMYIPALLLIVMLLRS 356 >A9U728.1 A9U728_PHYPA Predicted protein Length = 456 Score = 188 bits (477), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 124/351 (35%), Positives = 199/351 (56%), Gaps = 3/351 (0%) Query: 8 SALQLSFMIMLFEIGSTPLFLLGAKAKQDSWLAMCTGSAAGLVLLMLLMWIQYRSRHNDL 67 A +++ + L+E+GST LFLLG +AKQD+W+AM G+ AGL+LL++ + + DL Sbjct: 99 RASEIAISLSLYEVGSTTLFLLGGEAKQDAWIAMLLGAGAGLLLLLVYLLMYRLDAERDL 158 Query: 68 IGMLALHFGKIAGSALGGMYSLYFAYQSMRNVRDLGELTALTMLPNSPMSVTMLIFVGTA 127 M + G I G + YFAY++ RN RD+GEL TML +PMS+ + I V Sbjct: 159 FEMCRHYLGSIFGWIAAFALTAYFAYEASRNFRDIGELAIQTMLNRTPMSIIIFISVLIG 218 Query: 128 LYAIWKGTEVVFRLPEVLLPVVLSF-YAVLVLLLGIMGSLDFNHLAPVFENGFKPIADAA 186 Y + G FRL V+ V++F Y ++++++ + G L + PV ENGF P+ AA Sbjct: 219 SYITYLGPRAAFRL-SVIYAYVMAFGYVLILVMVPLTGLLHTKFMFPVLENGFAPVWRAA 277 Query: 187 LPDIVSFPFGQMIVFLMLWSLWDKPGVPVKSTVTAYLLISIFLIFMNALNIAVLGPSIAG 246 +P+IVSFPFGQ ++FL+ + K + T+ +YL ++FL +N L I VLGP IA Sbjct: 278 MPNIVSFPFGQTVLFLVYFKYAQKGKHLGRLTIVSYLATAVFLTVLNQLVILVLGPEIAT 337 Query: 247 TSQLPFLKTVRTLSSLKFIERLDILVTIQLFIGLLIKMMLFYFCSVKALTGLTGKSAKWW 306 P V+ + IER D L + LF+G+ +K + L +TG K W Sbjct: 338 IGTYPLFMVVQMMEVANVIERADALFVLLLFMGIGMKANFLLIGAGIGLQRITGIRYKLW 397 Query: 307 VFPVGAVIYGASFLERDYTQHIAIGLGPSL-KIDPVFQVAIPLLLAVSILL 356 V P+ ++G ++L +T++ GL ++ ++ P+ Q+ IP +L +++L+ Sbjct: 398 VIPLALTVFGLTYLSPTFTEYFRAGLKLTIEQLMPITQLVIPGILVIAMLI 448 >Q97TS5.1 Q97TS5_CLOAB Spore germination protein, GRKB Length = 361 Score = 160 bits (405), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 93/282 (32%), Positives = 156/282 (55%), Gaps = 1/282 (0%) Query: 6 QVSALQLSFMIMLFEIGSTPLFLLGAKAKQDSWLAMCTGSAAGLVLLMLLMWIQYRSRHN 65 ++S QL +I +FE+GSTP+F LG +A+Q++W+ + GL +L + +Q Sbjct: 3 RLSKHQLFTLIFVFEVGSTPIFALGIEAEQNAWIVVLLALLIGLFILWSYLELQKAFPTK 62 Query: 66 DLIGMLALHFGKIAGSALGGMYSLYFAYQSMRNVRDLGELTALTMLPNSPMSVTMLIFVG 125 L+ + GK G L +Y++ F + RN+R+ EL A+T++P++P+ + + F+G Sbjct: 63 GLVEIFTNILGKPLGLPLCILYAMGFIWGCGRNLREFIELIAMTVIPHTPLWIILFFFIG 122 Query: 126 TALYAIWKGTEVVFRLPEVLLPVVLSFYAVLVLLLGIMGSLDFNHLAPVFENGFKPIADA 185 +LY KG EV R E + P ++ F +L L+ I G + F +L P+ +G KPI Sbjct: 123 LSLYVSVKGFEVFARTTEFIFPTIIFFLIILFALISISGEVHFKNLTPILGDGLKPILK- 181 Query: 186 ALPDIVSFPFGQMIVFLMLWSLWDKPGVPVKSTVTAYLLISIFLIFMNALNIAVLGPSIA 245 +LP +V FPFG++ VFLM W + K++ A +L I L F +NI LG Sbjct: 182 SLPGVVWFPFGEIYVFLMYWHYVNDINTIKKASFKAVILSGILLCFTTMINICTLGAKYT 241 Query: 246 GTSQLPFLKTVRTLSSLKFIERLDILVTIQLFIGLLIKMMLF 287 + +P ++T+R ++ I LD++ I +F+G KM L+ Sbjct: 242 SIATIPLVETLRLINIGDIITHLDVIGVIIIFLGGFFKMTLY 283 >A7GUP4.1 A7GUP4_BACCN Spore germination protein Length = 364 Score = 159 bits (401), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 125/372 (33%), Positives = 196/372 (52%), Gaps = 31/372 (8%) Query: 4 KEQVSALQLSFMIMLFEIGSTPLFLLGAKAKQDSWLAMCTGSAAGLVLLMLLMWI----- 58 +E++ +QL +++M FE+GST ++ LG AKQD+WL + G G VL MWI Sbjct: 3 REKIGIMQLFYIMMAFEVGSTVIYGLGVDAKQDAWLVILVGMFCGFVL----MWIYTKLF 58 Query: 59 QYRSRHNDLIGMLALHFGKIAGSALGGMYSLYFAYQSMRNVRDLGELTALTMLPNSPMSV 118 +Y S + L M+ GK+ G L +Y YF Y + R +RD GEL T LP +P+ + Sbjct: 59 EYYS-GDTLTQMIPKIVGKLIGYPLNVIYIFYFIYIAARVLRDFGELITGTFLPKTPLII 117 Query: 119 TMLIFVGTALYAIWKGTEVVFRLPEVLLPVVLSFYAVLVLLLGIMGS---LDFNHLAPVF 175 + F+ +Y + G EV R+ E+ P + + V +L I+ S +D LAPV Sbjct: 118 VIGSFMLVIVYCLHGGIEVFGRMGEIFFPFL---FLVAILTWAIVYSSQVVDIEQLAPVL 174 Query: 176 ENGFKPIADAALPDIVSFPFGQMIVFLMLW-SLWDKPGVPVKSTVTAYLLISIFLIFMNA 234 E GF P+ +AA P +++FPFG+M++F+M W +L D VK +L++ L+ +N Sbjct: 175 EKGFGPVWEAAFPLVITFPFGEMVLFMMFWPALHDSR--KVKKLGMVVVLVAGILLTVNT 232 Query: 235 LNI-AVLGPSIAGTSQLPFLKTVRTLSSLKFIERLDILVTIQLFIGLLIKMMLF-YFCSV 292 ++I +V+GP++ P L VR S IER+D + I + IG K+ F Y +V Sbjct: 233 ISILSVIGPNLVKMKTYPLLTIVRMASIGNLIERIDAAIIITMMIGGFFKVGSFLYGAAV 292 Query: 293 KALTGLTGKSAKWWVFPVGAVIYGASFL-ERDYTQHIAIGLGPSLKIDPVF-----QVAI 346 KS + V GAV+ S + +Y +H IG +K P+F Q+ I Sbjct: 293 GTAQLFRLKSYRSIVVVFGAVVVLLSIMIATNYMEHEEIG----IKKVPIFLHIPLQIVI 348 Query: 347 PLLLAVSILLRS 358 P++L V ++R Sbjct: 349 PVILLVIAMIRK 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,046,602,220 Number of extensions: 82068793 Number of successful extensions: 275700 Number of sequences better than 1.0e-05: 459 Number of HSP's gapped: 275582 Number of HSP's successfully gapped: 459 Length of query: 365 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 228 Effective length of database: 1,770,017,148 Effective search space: 403563909744 Effective search space used: 403563909744 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)