BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0040.0005.p (291 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KBQ9.1 Q9KBQ9_BACHD Sugar transport system (Permease) (Binding... 305 2e-81 C5C4A9.1 C5C4A9_9MICO Binding-protein-dependent transport system... 231 8e-59 B7DT28.1 B7DT28_9BACL Binding-protein-dependent transport system... 229 2e-58 B3K641.1 B3K641_9BACI Binding-protein-dependent transport system... 228 5e-58 A4U8U0.1 A4U8U0_9BACT Sugar permease 223 2e-56 >Q9KBQ9.1 Q9KBQ9_BACHD Sugar transport system (Permease) (Binding protein dependent transporter) Length = 285 Score = 305 bits (782), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 150/281 (53%), Positives = 198/281 (70%) Query: 11 AWGSKKQARERTFKGFAYLFLSAGALVMAVPFLWMLSTSLKQQGMEFEMPPKWIPQHFEW 70 +W K K +Y FL G L+MAVPFLWMLSTSLK G F MPP++IP+ + Sbjct: 5 SWYQSKSKMNTIKKILSYTFLMIGGLIMAVPFLWMLSTSLKTPGAIFAMPPEFIPREITF 64 Query: 71 SNYHFVLFESDIVQGFVNTXXXXXXPCLVGXXXXXXXXXXXXXXXXXXRNLLFGLLLATM 130 NY V E+D++ GF NT +G R+ LF L+ATM Sbjct: 65 ENYIRVFTEADLLLGFRNTMIIIIPTTAIGIFTSSLAAFAFAKLNFPGRDKLFFALIATM 124 Query: 131 MIPGVVTMIPTFMLFKNIGWIDSWMPLIIPGMFGAAAAVFLLRQFFLTIPRELEDAAKVD 190 M+PG+VT++P F+LF+ +GW+D++ PL+IPG FGAA VF LRQFF+TIP +L DAAK+D Sbjct: 125 MLPGIVTLVPQFILFRELGWLDTFKPLMIPGFFGAAMTVFFLRQFFMTIPNDLIDAAKID 184 Query: 191 GMNPFHTFLRIMLPLSKPALITQGLLNFIAGYNDYLGPLIYINSPEKYTLQLVLASFQGF 250 G++ F F +IM+PL+KPA++TQ +L +AGYND++GPLIY+NSPE +TLQLVLASF+G+ Sbjct: 185 GLSFFGIFRKIMVPLAKPAIVTQVILGLLAGYNDFMGPLIYLNSPENFTLQLVLASFRGY 244 Query: 251 YTSQWTYIMAGSVLALIPTLLVFFFAQKYFVEGIALTGMKG 291 YTS+W IMAGSV+ALIPTL+ FFFAQK F+EGI +TG+KG Sbjct: 245 YTSEWGLIMAGSVIALIPTLVAFFFAQKQFIEGIVMTGVKG 285 >C5C4A9.1 C5C4A9_9MICO Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 231 bits (588), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 122/296 (41%), Positives = 178/296 (60%), Gaps = 6/296 (2%) Query: 1 VSTRTTTANGAWGSKKQ----ARERTFKGFA-YLFLSAGALVMAVPFLWMLSTSLKQQGM 55 V RT + +++ R R K Y FL GA+ +P +W++ ++L G Sbjct: 4 VIARTASEQQVPAERRRPAGTGRSRRLKRVGIYAFLVLGAIPTLLPLIWLVRSALMTSGQ 63 Query: 56 EFEMPPKWIPQHFEWSNYHFVLFESDIVQGFVNTXXXXXXPCLVGXXXXXXXXXXXXXXX 115 F PP+WIP W N+ L + FVNT + Sbjct: 64 IFISPPEWIPDPVAWDNFAGALTAVPFGRYFVNTMIIEVGVLVGTLLSCSFAAFSFARLR 123 Query: 116 XXXRNLLFGLLLATMMIPGVVTMIPTFMLFKNIGWIDSWMPLIIPGMFGAAA-AVFLLRQ 174 +N++FGLL+ +M+P VT+IPTF+ ++ +G +++++PLI+P FG A +FLLRQ Sbjct: 124 WRFKNVVFGLLMTGVMLPYAVTLIPTFLFWQAVGAVNTYVPLILPAFFGTAVFNIFLLRQ 183 Query: 175 FFLTIPRELEDAAKVDGMNPFHTFLRIMLPLSKPALITQGLLNFIAGYNDYLGPLIYINS 234 FFL++P EL+++A +DG NPF + RI+LPLSKPAL+ G+ F+ +NDYLGPLIY+N Sbjct: 184 FFLSLPYELDESAYIDGANPFQVYWRIVLPLSKPALMVVGIFTFLGVWNDYLGPLIYLND 243 Query: 235 PEKYTLQLVLASFQGFYTSQWTYIMAGSVLALIPTLLVFFFAQKYFVEGIALTGMK 290 + YTL + LASFQG YT+QW Y+MA + + L+P +L+F AQKYFVEGIALTG+K Sbjct: 244 SDMYTLAMGLASFQGTYTAQWGYLMAAATVVLLPIILLFAIAQKYFVEGIALTGLK 299 >B7DT28.1 B7DT28_9BACL Binding-protein-dependent transport systems inner membrane component Length = 292 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 115/279 (41%), Positives = 165/279 (59%) Query: 13 GSKKQARERTFKGFAYLFLSAGALVMAVPFLWMLSTSLKQQGMEFEMPPKWIPQHFEWSN 72 ++ + +R + YL L ++ +PF W++ +SLK F PP W P+ EWSN Sbjct: 14 STRARFTQRLHRFVTYLLLVGTSICFLIPFAWLVISSLKPNDQIFVFPPTWWPKPVEWSN 73 Query: 73 YHFVLFESDIVQGFVNTXXXXXXPCLVGXXXXXXXXXXXXXXXXXXRNLLFGLLLATMMI 132 Y L + +N+ + R LLF LLLATMM+ Sbjct: 74 YIQALTSQPFGRYALNSLLIALVNVIGNLVSNSFVAYGFARFRFPGRRLLFMLLLATMMV 133 Query: 133 PGVVTMIPTFMLFKNIGWIDSWMPLIIPGMFGAAAAVFLLRQFFLTIPRELEDAAKVDGM 192 P V ++P F+LF ++GWID+++PL +P FG+A VFLLRQFF+TIP ELE+AA++DG Sbjct: 134 PSQVLLVPQFILFHDLGWIDTFLPLTVPSFFGSAFYVFLLRQFFMTIPVELEEAARIDGA 193 Query: 193 NPFHTFLRIMLPLSKPALITQGLLNFIAGYNDYLGPLIYINSPEKYTLQLVLASFQGFYT 252 P F ++LPL +PAL + +F +ND+L PLIY++ P K+TLQL LA FQG + Sbjct: 194 GPLRIFFSVVLPLIRPALTAVAIFSFQGAWNDFLTPLIYLSDPSKFTLQLGLAQFQGSFH 253 Query: 253 SQWTYIMAGSVLALIPTLLVFFFAQKYFVEGIALTGMKG 291 + W IMA SV+ ++P ++FF AQKYFV+GI +TG KG Sbjct: 254 TDWNLIMAASVVVMLPMAIIFFVAQKYFVQGITMTGTKG 292 >B3K641.1 B3K641_9BACI Binding-protein-dependent transport systems inner membrane component Length = 277 Score = 228 bits (581), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 117/269 (43%), Positives = 167/269 (62%), Gaps = 1/269 (0%) Query: 24 KGFAYLFLSAGALVMAVPFLWMLSTSLKQQGMEFEMPPKWIPQHFEWSNYHFVLFESDIV 83 KG YL L+AG + +P +W++ +SL G FE+PP WIP+ + SNY L Sbjct: 9 KGLIYLVLAAGGIFSLLPLVWLIRSSLMNMGQIFELPPVWIPRPVQLSNYSEALTILPFG 68 Query: 84 QGFVNTXXXXXXPCLVGXXXXXXXXXXXXXXXXXXRNLLFGLLLATMMIPGVVTMIPTFM 143 Q F+NT L RN++FGLLL++MM+P VT+IPTF+ Sbjct: 69 QYFMNTAIIVVFSVLGVIVTSSISAYSFARMKWRGRNMVFGLLLSSMMLPYAVTLIPTFI 128 Query: 144 LFKNIGWIDSWMPLIIPGMFGAAA-AVFLLRQFFLTIPRELEDAAKVDGMNPFHTFLRIM 202 + +G +S++PLI P FG A +FLLRQF+L+IPR+L++AA VDG F I+ Sbjct: 129 GWSKVGLTNSFIPLIAPAWFGGGAFNIFLLRQFYLSIPRDLDEAAYVDGAGHLRIFRSII 188 Query: 203 LPLSKPALITQGLLNFIAGYNDYLGPLIYINSPEKYTLQLVLASFQGFYTSQWTYIMAGS 262 LPL+KPALI GL +F+ +ND+LGPL+Y+N KYTL L L F+G Y+++W +MA + Sbjct: 189 LPLTKPALIVVGLFSFLTAWNDFLGPLVYLNDETKYTLALGLQQFKGMYSAEWHLMMAAA 248 Query: 263 VLALIPTLLVFFFAQKYFVEGIALTGMKG 291 + L+P ++VFF Q+YF+EGI LTG+K Sbjct: 249 TVVLLPAIIVFFIGQRYFIEGITLTGIKA 277 >A4U8U0.1 A4U8U0_9BACT Sugar permease Length = 288 Score = 223 bits (567), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 114/277 (41%), Positives = 168/277 (60%), Gaps = 1/277 (0%) Query: 16 KQARERTFKGFAYLFLSAGALVMAVPFLWMLSTSLKQQGMEFEMPPKWIPQHFEWSNYHF 75 + AR R K YL G+++M +P W++ +S+ G F PP+WIP + W NY Sbjct: 12 RTARRRLGKTVVYLLCILGSVLMLIPLAWLVRSSVMSLGQIFAFPPEWIPSPWRWENYPQ 71 Query: 76 VLFESDIVQGFVNTXXXXXXPCLVGXXXXXXXXXXXXXXXXXXRNLLFGLLLATMMIPGV 135 L + FVNT L R+++FG+LL T+M+P Sbjct: 72 ALTSIPFFRYFVNTLVILVPSVLGTVITSTLAAYGFSRLQWPGRDIVFGILLTTLMLPYA 131 Query: 136 VTMIPTFMLFKNIGWIDSWMPLIIPGMFGAAA-AVFLLRQFFLTIPRELEDAAKVDGMNP 194 VT+IPTF+L+ ++G + ++ PL++P FG +FLLRQFF+T+P+EL++AA +DG +P Sbjct: 132 VTLIPTFLLWASLGLVGTYWPLVVPHFFGGGIFYIFLLRQFFMTLPKELDEAAIIDGASP 191 Query: 195 FHTFLRIMLPLSKPALITQGLLNFIAGYNDYLGPLIYINSPEKYTLQLVLASFQGFYTSQ 254 +++PLS+PALIT + + +ND+L PLIYIN ++TL L LA F G YTSQ Sbjct: 192 PQVLWYVIVPLSRPALITVVIFATLFEWNDFLEPLIYINRSSQHTLALGLAEFTGLYTSQ 251 Query: 255 WTYIMAGSVLALIPTLLVFFFAQKYFVEGIALTGMKG 291 W +MA S + ++P L++FFFAQ+YF+EGIALTG KG Sbjct: 252 WHLLMAASTVVILPVLVLFFFAQRYFIEGIALTGTKG 288 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,575,262,622 Number of extensions: 60047137 Number of successful extensions: 214635 Number of sequences better than 1.0e-05: 5844 Number of HSP's gapped: 206436 Number of HSP's successfully gapped: 5845 Length of query: 291 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 157 Effective length of database: 1,798,280,193 Effective search space: 282329990301 Effective search space used: 282329990301 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)