BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0040.0003.p (666 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBZ5.1 B3KBZ5_9BACI Alpha-L-arabinofuranosidase B 1040 0.0 C4DDC4.1 C4DDC4_9ACTO Putative uncharacterized protein 149 7e-34 C1WVM2.1 C1WVM2_9ACTO Putative carbohydrate-binding protein 147 3e-33 A0ACE9.1 A0ACE9_STRAM Putative bifunctional protein (Secreted su... 145 9e-33 A4FLZ6.1 A4FLZ6_SACEN Bifunctional protein (Secreted sugar bindi... 135 2e-29 >B3KBZ5.1 B3KBZ5_9BACI Alpha-L-arabinofuranosidase B Length = 667 Score = 1040 bits (2689), Expect = 0.0, Method: Compositional matrix adjust. Identities = 504/667 (75%), Positives = 580/667 (86%), Gaps = 1/667 (0%) Query: 1 MRISMKKAMLLTLIAALLVMGISVAPVQAATTGTVISSSAAEPEPYYARAVKLDNGNLLS 60 M +K+ LL +I L + ++ QAA GTV+ S+ AEPEPYY RAVKLDNG++L+ Sbjct: 1 MNTRLKRFGLLAVIVMLFMSTLAAPTAQAAAIGTVVYSNPAEPEPYYVRAVKLDNGDILT 60 Query: 61 TFTRRFPGNTDWAGMKAFPFYRSTDGGATWSLLSEIDPNNYGLDRNQQGMTTLYVLPQKA 120 TFT RFPGN W+GM+ FPFY+STD GATWSL SEIDPN+YGL+RNQQGMTTLYVLPQ+ Sbjct: 61 TFTPRFPGNAGWSGMQPFPFYKSTDNGATWSLFSEIDPNDYGLNRNQQGMTTLYVLPQQV 120 Query: 121 GDYPAGTLLFASTDWNNSVPYTIHIWRSTDNGATWQFHSNLAARGTAGTQRTWEPEFSVT 180 GDYPAGTLLFASTDW+NS PYTIHIWRSTDNGATWQFHSNLAARGT GT+RTWEPEF++T Sbjct: 121 GDYPAGTLLFASTDWDNSAPYTIHIWRSTDNGATWQFHSNLAARGTEGTKRTWEPEFAIT 180 Query: 181 SDGRLICYYSDERQPGYNQAIAQEISSDGGLTWGNYSIIVGNSSNWDWRPGMPRVVKVKN 240 +DGRLICYYSDER+PGYNQAIAQEISSDGGLTWGNYSIIVGN ++W+WRPGMPRV++ KN Sbjct: 181 ADGRLICYYSDERKPGYNQAIAQEISSDGGLTWGNYSIIVGNQADWNWRPGMPRVIQAKN 240 Query: 241 GTYFMFFEMLGAAPNFAVRYKTSANGVDWGSATDLGNVVGTGIYRASQTPEIAYIDNGTA 300 G+YFMFFEMLGAAPNFAVR+KTS +G++WGS TDLG VVGTGIYRASQTPE+AYID+GTA Sbjct: 241 GSYFMFFEMLGAAPNFAVRFKTSPDGINWGSPTDLGEVVGTGIYRASQTPEVAYIDDGTA 300 Query: 301 NGRLYVRGMTDVISSANKMFTSGDYGATWTQVDAPLTVKGSNQNTPAAWSGTLLPLSHSM 360 NGR YVRGMTDV+SSANKMFTS DYGATWTQ+DAPLTVKG+NQ+TPAAWSGTLLPL S+ Sbjct: 301 NGRFYVRGMTDVVSSANKMFTSADYGATWTQMDAPLTVKGTNQSTPAAWSGTLLPLGPSL 360 Query: 361 LLEVNTVKVGDRNEIRANVAQVNGDSSIVSGGIYKXXXXXXXXXXXXAGGGSPAGTEIIQ 420 LLEVNTVKVG RNEIRANVAQV+ + SIVSGG YK AGGGSPAGT +IQ Sbjct: 361 LLEVNTVKVGSRNEIRANVAQVDKEFSIVSGGTYKLVNQNNQLLLDNAGGGSPAGTNVIQ 420 Query: 421 WSDLGADTQWWRLDYRNSGFFRGMSVNNSLVWEDPDGATAPGTKVILGTNNDLDTQRWKF 480 W+DLGADTQWWRLDYRN GFFR M+VNNSL+ ++P+GAT PGT VI+ TNN+ DTQRWKF Sbjct: 421 WNDLGADTQWWRLDYRNGGFFRAMNVNNSLILDNPNGATTPGTNVIMWTNNEWDTQRWKF 480 Query: 481 AHKGSSYYTVMNGHSGLMLDNAGGGSPAGTKVIQWGANSLDTQNWKLVRVDTEIPVNQLE 540 HKG+ YYT MN SGLMLDNAG GSPAGTKVIQW AN LDTQNWK VRVD EIPVNQLE Sbjct: 481 THKGNGYYTSMNERSGLMLDNAGAGSPAGTKVIQWTANGLDTQNWKAVRVDAEIPVNQLE 540 Query: 541 SFNM-HSYVQYQDGRGRIDGGQYSAESQWRLVKGLANESSVSIESVNKPGFYLRHRDGKV 599 S+N+ +SY++Y DGRG+IDGGQYSAESQWR+V GLA+ S+VSIESV+ PG YLRHRDGK+ Sbjct: 541 SYNIPNSYIRYHDGRGKIDGGQYSAESQWRMVPGLADPSAVSIESVSFPGQYLRHRDGKI 600 Query: 600 WLEANDNSALFKNDATWQLRAGLTGSWAASFESYNIPGAYMRHRNGLLEISTISTAVDQQ 659 WLE+N+++ALFK DATW+LR G + WAASFESYNI G ++RHR+G+LEIS I+T +DQ+ Sbjct: 601 WLESNNDTALFKQDATWKLRTGFSSPWAASFESYNISGTFIRHRDGVLEISPITTTLDQK 660 Query: 660 DATFYVK 666 DATFYVK Sbjct: 661 DATFYVK 667 >C4DDC4.1 C4DDC4_9ACTO Putative uncharacterized protein Length = 394 Score = 149 bits (377), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 122/334 (36%), Positives = 179/334 (53%), Gaps = 40/334 (11%) Query: 47 YARAVKLD-----NGNLLSTFTRRFPGNTDWAGMKAFPFYRSTDGGATWSLLSEI-DPNN 100 Y RAV+L+ NG +++ F GN D G Y STD GAT++ + ++ DP+ Sbjct: 48 YPRAVRLEHNGDANGRVVAGVVS-FVGNGDGIGA----IYESTDDGATFAEVGQVADPDA 102 Query: 101 YGLDRNQQGMT--TLYVLPQKAGDYPAGTLLFASTDWNNSVP--YTIHIWRSTDNGATWQ 156 G QG+ TL+ LP++ GD AGTLL+A++ + ++ IWRS D G TW Sbjct: 103 AG----GQGLCCATLFELPRQVGDMRAGTLLWAASVGADEPDRRMSLRIWRSDDLGRTW- 157 Query: 157 FHSNLAARGTAGTQR-TWEPEFSVTSDGRLICYYSDERQPGYNQAIAQEISSDGGLTWGN 215 S+L+A TA + WEPEFSV +DG L+C+Y+DE P ++Q + S+D G+TW + Sbjct: 158 --SHLSACATAPDAKGLWEPEFSVAADGALVCHYADETDPAHSQKLMHARSAD-GVTWTD 214 Query: 216 YSIIVGNSSNWDWRPGMPRVVKVKNGTYFMFFEMLGAAPNFAV--RYKTSANGVDWGSAT 273 +S V ++ D RPGMP V ++ NGTYFM FE+ FA Y+TSA+G DWG Sbjct: 215 HSPTVASTLASD-RPGMPVVRQLPNGTYFMSFEICAPDGQFACVTHYRTSADGWDWGDPA 273 Query: 274 DLGNVVGT--GIYRASQTPEIAY-IDNGTANG-RLYVRGMT-----DVISSAN--KMFTS 322 LG T G Y P I + D + NG RL++ G +++ N + T+ Sbjct: 274 HLGFQPETADGKYF-KHAPTITWKADPDSPNGARLFLIGQILFNADGSVAAENGAAVLTN 332 Query: 323 GDYGA-TWTQVDAPLTVKGSNQNTPAAWSGTLLP 355 G+ W + +P+ V + N +S TLLP Sbjct: 333 SQGGSGPWELIPSPVAVNAPSNNYCPNYSSTLLP 366 >C1WVM2.1 C1WVM2_9ACTO Putative carbohydrate-binding protein Length = 539 Score = 147 bits (372), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 121/353 (34%), Positives = 177/353 (50%), Gaps = 37/353 (10%) Query: 47 YARAVKLD-----NGNLLSTFTRRFPGNTDWAGMKAFPFYRSTDGGATWSLLSEI-DPNN 100 Y RA++L NG +L++ F GN D G Y STD GA + + + DP Sbjct: 63 YPRALRLQHNGAANGRILASVVT-FVGNGDGLGA----IYESTDAGAAFRQVGAVADPE- 116 Query: 101 YGLDRNQQGMTTLYVLPQKAGDYPAGTLLFASTDWNNSVPYTIHIWRSTDNGATWQFHSN 160 D TLY LPQ G PAGTLL+A + + P ++ IWRS D G TW + S+ Sbjct: 117 -AADGQGLCCATLYELPQAVGQLPAGTLLWAGSVGASDRPMSLRIWRSNDLGRTWSYLSS 175 Query: 161 LAARGTAGTQRTWEPEFSVTSDGRLICYYSDERQPGYNQAIAQEISSDGGLTWGNYSIIV 220 A + WEPEFS+ +DGRL+C+Y+DE NQ + + SSD G+ W + V Sbjct: 176 CA--DAVNDRGLWEPEFSIAADGRLVCHYADETDAAQNQKLMEVRSSD-GIHWTDRRATV 232 Query: 221 GNSSNWDWRPGMPRVVKVKNGTYFMFFEM--LGAAPNFAVRYKTSANGVDWGSATDLGNV 278 S + RPGMP V K+ NGTYF+ +E+ LG + AV Y+TS +G +WG G Sbjct: 233 -TSDDPRHRPGMPVVRKLPNGTYFLTYEICALGGQYDCAVFYRTSRDGWNWGDPKQRGIQ 291 Query: 279 VGT--GIYRASQTPEIAYIDN-GTANGRLYVRGMTDVISSANKMFTSGD----YGAT--- 328 T G Y + TP +A+ N G +G+L + G ++ +A+ G+ +G T Sbjct: 292 PRTADGKY-FTHTPTVAWSANEGQRDGKLLLIGQ--ILHNADGSVADGNGQTIFGNTENG 348 Query: 329 ---WTQVDAPLTVKGSNQNTPAAWSGTLLPLSHS-MLLEVNTVKVGDRNEIRA 377 W ++ AP+ V N +S L+P + +LE+ + D E +A Sbjct: 349 AGPWYEIAAPVEVPSPYNNFCPNYSSPLVPSENGRRVLEIASAWAAD-GECKA 400 >A0ACE9.1 A0ACE9_STRAM Putative bifunctional protein (Secreted sugar binding protein/sugar hydrolase) Length = 419 Score = 145 bits (367), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 123/362 (33%), Positives = 175/362 (48%), Gaps = 41/362 (11%) Query: 21 GISVAPVQAAT----TGTVISSSAAEPEPYYARAVKLD-----NGNLLSTFTRRFPGNTD 71 G+ APV A + T + E Y RAV+L NG +L+ GN Sbjct: 39 GVPSAPVPPAVADVPSPTATGTPLREGTGLYPRAVRLAHSGAANGRVLAGVVTFDGGN-- 96 Query: 72 WAGMKAFPFYRSTDGGATWSLLSEI-DPNNYGLDRNQQGMT--TLYVLPQKAGDYPAGTL 128 G+ A + STD G T+ + + DP + QG+ TL+ LP+ G PAGTL Sbjct: 97 --GVGAI--HESTDSGVTFREVGRVSDPESAA----GQGLCCATLFELPRPVGAMPAGTL 148 Query: 129 LFASTDWNNSVP--YTIHIWRSTDNGATWQFHSNLAARGTAGTQRTWEPEFSVTSDGRLI 186 ++AS+ + + IWRS D G +W + S+ A +GT WEPEFSV DG L+ Sbjct: 149 IWASSAGQDEQDRRMALRIWRSDDVGRSWSYLSSCAV--ASGTGGLWEPEFSVAGDGALV 206 Query: 187 CYYSDERQPGYNQAIAQEISSDGGLTWGNYSIIVGNSSNWDWRPGMPRVVKVKNGTYFMF 246 C+Y+DE PG++Q + S D G+ W + V + + D RPGMP V K+ NGTYFM Sbjct: 207 CHYADETDPGHSQKLVAARSYD-GVHWEGHHDTVASGWSPD-RPGMPVVRKLPNGTYFMS 264 Query: 247 FEML--GAAPNFAVRYKTSANGVDWGSATDLGNVVGTG---IYRASQTPEIAYIDNGTAN 301 +E+ G V ++TSA+G DWG+ LG T +RA+ T A +G N Sbjct: 265 YEICNPGGQYQCVVHFRTSADGWDWGNPASLGFRPETADGRYFRAAPTIAWAPSPDGAPN 324 Query: 302 GRLYVRGM-------TDVISSANKMFTSGDYGA-TWTQVDAPLTVKGSNQNTPAAWSGTL 353 GRL + G T S + + + G+ W + AP+TV N +S L Sbjct: 325 GRLLLVGQRLLNRDGTTATGSGRTILVNAENGSGPWHEAPAPVTVPDPQVNYCQNYSSPL 384 Query: 354 LP 355 LP Sbjct: 385 LP 386 >A4FLZ6.1 A4FLZ6_SACEN Bifunctional protein (Secreted sugar binding protein/sugar hydrolase) Length = 429 Score = 135 bits (339), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 109/334 (32%), Positives = 163/334 (48%), Gaps = 40/334 (11%) Query: 47 YARAVKLDNGNLLSTFTRRFPGNTDWAGMKAFPFYRSTDGGATWSLLSEI-DPNNYGLDR 105 Y R V+L++G D AG Y+S D G ++ EI DP Sbjct: 62 YPRLVRLEHGPAKGRVLASMTTYVDRAGRAVI--YQSDDDGRSFQQAGEIRDPEG----E 115 Query: 106 NQQGM--TTLYVLPQKAGDYPAGTLLFAST-------DWNNSVPYTIHIWRSTDNGATWQ 156 N++GM +TLY LPQ+ GD PAGTLL+A T D + +I +WRS D G TW+ Sbjct: 116 NEKGMCCSTLYELPQRVGDMPAGTLLWAGTAGIGADADVREA---SIRLWRSDDQGRTWR 172 Query: 157 FHSNL--AARGTAGTQRTWEPEFSVTSDGRLICYYSDERQPGYNQAIAQEISSDGGLTWG 214 F S + A RG WEPEF+V+ DG L+ +YSD+ P ++Q + Q S D G+ W Sbjct: 173 FLSTIVDAPRGPG----VWEPEFTVSEDGDLVAFYSDDGDPKHDQKMVQVRSRD-GVNWT 227 Query: 215 NYSIIVGNSSNWDWRPGMPRVVKVKNGTYFMFFEMLGAAPNF--AVRYKTSANGVDWGSA 272 + + N + RPGM V ++ +GTY M +E+ P+ V + S +G DWG Sbjct: 228 DVKETIKN-DKFTVRPGMAGVRQLPDGTYVMVYEVCNYDPDHICTVHMRKSEDGWDWGDP 286 Query: 273 TDLGNVVGTGI-YRASQTPEIAYIDNGTANGRLYV--------RGMTDVISSANKMFT-- 321 DLG + + + TP IA+ NGRL + RG + + T Sbjct: 287 YDLGTEITSDTGAQPLGTPTIAWAPGPGRNGRLLLAYQLLALDRGGLAPGNGRTLLVTDD 346 Query: 322 SGDYGATWTQVDAPLTVKGSNQNTPAAWSGTLLP 355 + G W ++ AP+ +K ++ NT +S +++P Sbjct: 347 PANPGKPWREMPAPVHIKYNHGNTCRNFSPSVVP 380 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,333,315,093 Number of extensions: 195774873 Number of successful extensions: 402334 Number of sequences better than 1.0e-05: 192 Number of HSP's gapped: 402014 Number of HSP's successfully gapped: 200 Length of query: 666 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 523 Effective length of database: 1,713,491,058 Effective search space: 896155823334 Effective search space used: 896155823334 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 135 (56.6 bits)