BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0030.p (277 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDP8.1 B3KDP8_9BACI Binding-protein-dependent transport system... 374 e-102 B3K4S4.1 B3K4S4_9BACI Binding-protein-dependent transport system... 355 2e-96 A6CLU7.1 A6CLU7_9BACI Multiple sugar-binding transport system pe... 304 7e-81 Q2BAB3.1 Q2BAB3_9BACI Multiple sugar-binding transport system pe... 288 3e-76 C4L2X2.1 C4L2X2_EXISA Binding-protein-dependent transport system... 288 5e-76 >B3KDP8.1 B3KDP8_9BACI Binding-protein-dependent transport systems inner membrane component Length = 279 Score = 374 bits (960), Expect = e-102, Method: Compositional matrix adjust. Identities = 172/268 (64%), Positives = 222/268 (82%) Query: 10 NIGIEVLMILLALLFLSPFYFLLANSVKSFGEILSNAASWPQEFMWSNYTNAWKLARFSE 69 I ++++M+++AL+FL+PFYFLL NSVKS G+I+ +AA+WP F + NY+ AW++ RF E Sbjct: 12 TIVLQIVMVIVALIFLAPFYFLLVNSVKSLGDIMVDAANWPTAFHFDNYSKAWEMTRFPE 71 Query: 70 AFRNXXXXXXXXXXXXXXFSAMAAYRMVRANTRFNQILLLLFVAAMVVPFQTIMIPILKV 129 AF N SAMAAYRMVR+NTRFN+I+ +LFV+AMV+PFQ+IMIP+L+V Sbjct: 72 AFTNSIIITVISNLIIALLSAMAAYRMVRSNTRFNRIVFMLFVSAMVIPFQSIMIPLLQV 131 Query: 130 VNILGVNNSFAGLIISNLGLSIPMAIFLFHGFIKSVPLEIEEAATVDGCNPISAFFRIVL 189 +N LGVNNS GLI+S LGL IP+++FLFHGF+K +PLEIEEAATVDG +P F RIV+ Sbjct: 132 INFLGVNNSIVGLILSYLGLGIPLSVFLFHGFVKGIPLEIEEAATVDGASPFRVFARIVM 191 Query: 190 PLLKPMLMTIIVLNALGIWNDYLLPSLILQAPGLRTIPLATFSFFGQYTKQWDMALPALT 249 P+LKPM++T+I+LN L +WNDYLLPSLILQ+P LRTIPLATF+FFGQY+KQWDMALPAL Sbjct: 192 PMLKPMMVTVIILNCLWVWNDYLLPSLILQSPELRTIPLATFAFFGQYSKQWDMALPALV 251 Query: 250 LGVAPIVIFYLFMQRYIVEGIAAGSVKG 277 LG+ PI+IF+L +Q+YIVEG+AAGSVKG Sbjct: 252 LGITPIIIFFLSLQKYIVEGVAAGSVKG 279 >B3K4S4.1 B3K4S4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 278 Score = 355 bits (912), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 166/278 (59%), Positives = 213/278 (76%), Gaps = 1/278 (0%) Query: 1 MKAKSKGRLN-IGIEVLMILLALLFLSPFYFLLANSVKSFGEILSNAASWPQEFMWSNYT 59 M+ K RLN + E++M+++ALLFL PFYFL NSVK+FGE+L+++A P F WSNY+ Sbjct: 1 METTRKYRLNTMATEIIMVIVALLFLVPFYFLFVNSVKTFGELLTDSAGLPTTFEWSNYS 60 Query: 60 NAWKLARFSEAFRNXXXXXXXXXXXXXXFSAMAAYRMVRANTRFNQILLLLFVAAMVVPF 119 AW + F +AF N SAM AY+MVR NTRFNQI+ LFVAAMV+PF Sbjct: 61 RAWDITNFPQAFWNSLVVTVASNLLLAMLSAMTAYQMVRRNTRFNQIMFALFVAAMVIPF 120 Query: 120 QTIMIPILKVVNILGVNNSFAGLIISNLGLSIPMAIFLFHGFIKSVPLEIEEAATVDGCN 179 Q+IMIP++KV LG+NNS GLII LG +P+++FLFHGF+KS+P+EIEEAATVDG + Sbjct: 121 QSIMIPLVKVTATLGINNSLYGLIICYLGFGVPLSVFLFHGFVKSIPMEIEEAATVDGSS 180 Query: 180 PISAFFRIVLPLLKPMLMTIIVLNALGIWNDYLLPSLILQAPGLRTIPLATFSFFGQYTK 239 FFR+V PL+KPM +T+I+LN L IWNDYLLPSLILQ+ LRTIP+ATF+FFGQYTK Sbjct: 181 GYGVFFRVVFPLMKPMFVTVIILNTLWIWNDYLLPSLILQSAELRTIPIATFAFFGQYTK 240 Query: 240 QWDMALPALTLGVAPIVIFYLFMQRYIVEGIAAGSVKG 277 QWD+ALPAL LG+ P+++F+L MQ+YI+EGI G+VKG Sbjct: 241 QWDLALPALVLGIMPVILFFLAMQKYIIEGITQGAVKG 278 >A6CLU7.1 A6CLU7_9BACI Multiple sugar-binding transport system permease Length = 275 Score = 304 bits (778), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 141/264 (53%), Positives = 196/264 (74%) Query: 13 IEVLMILLALLFLSPFYFLLANSVKSFGEILSNAASWPQEFMWSNYTNAWKLARFSEAFR 72 +E++ I + L+FL PFYF+L NSVK F IL +AA+WP+EF++SNY W++ F AF Sbjct: 12 LEIIGITVGLIFLIPFYFVLVNSVKPFAAILIDAAAWPKEFLFSNYAKVWEIINFPRAFW 71 Query: 73 NXXXXXXXXXXXXXXFSAMAAYRMVRANTRFNQILLLLFVAAMVVPFQTIMIPILKVVNI 132 N S+MAA++MVR +F++IL ++FV+AMV+PFQT+MIP++K+ Sbjct: 72 NSLVITVFSNIGLVIISSMAAWKMVRTPGKFSKILFIIFVSAMVIPFQTVMIPLMKLGGA 131 Query: 133 LGVNNSFAGLIISNLGLSIPMAIFLFHGFIKSVPLEIEEAATVDGCNPISAFFRIVLPLL 192 L + NS GLII G +P+++FLFHGF+K+VP+EIEE+A +DGC+ F+RIV PLL Sbjct: 132 LNLTNSIPGLIIMYFGFGVPLSLFLFHGFVKTVPMEIEESAMIDGCSQFGVFWRIVFPLL 191 Query: 193 KPMLMTIIVLNALGIWNDYLLPSLILQAPGLRTIPLATFSFFGQYTKQWDMALPALTLGV 252 KP+ +T+I+LN L IWNDYLLP L+LQ LRTIPLA SFF QYTKQWDM L AL LG+ Sbjct: 192 KPITVTVIILNTLWIWNDYLLPLLVLQDAELRTIPLAASSFFAQYTKQWDMGLAALVLGI 251 Query: 253 APIVIFYLFMQRYIVEGIAAGSVK 276 P++IF+LF+Q++I++GIA+GS+K Sbjct: 252 TPVIIFFLFLQKHIIKGIASGSIK 275 >Q2BAB3.1 Q2BAB3_9BACI Multiple sugar-binding transport system permease Length = 275 Score = 288 bits (738), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 133/250 (53%), Positives = 187/250 (74%) Query: 27 PFYFLLANSVKSFGEILSNAASWPQEFMWSNYTNAWKLARFSEAFRNXXXXXXXXXXXXX 86 PFYF+ NSVKSF EIL +AA++P+E +++NY+ W++ F AF N Sbjct: 26 PFYFVAVNSVKSFAEILLDAAAFPKEVLFANYSKVWEIINFPRAFWNSLIITVLSNIGLV 85 Query: 87 XFSAMAAYRMVRANTRFNQILLLLFVAAMVVPFQTIMIPILKVVNILGVNNSFAGLIISN 146 S+MAA++MVR RF++IL +LFV+AMV+PFQT+MIP++K+ L + NS GLI+ Sbjct: 86 IISSMAAWKMVRTPGRFSKILFILFVSAMVIPFQTVMIPLMKLGGALNMTNSIPGLIMMY 145 Query: 147 LGLSIPMAIFLFHGFIKSVPLEIEEAATVDGCNPISAFFRIVLPLLKPMLMTIIVLNALG 206 G +P+++FL+HGF+K+VP EIEE+A +DGC+ F+RIV PLLKP+ +T+++LN L Sbjct: 146 FGFGVPLSLFLYHGFVKTVPREIEESALIDGCSQFGVFWRIVFPLLKPITVTVVILNTLW 205 Query: 207 IWNDYLLPSLILQAPGLRTIPLATFSFFGQYTKQWDMALPALTLGVAPIVIFYLFMQRYI 266 IWND+LLP L+LQ LRTIPLAT SFF QYTKQWDM L AL LG+ P++IF+LF+Q++I Sbjct: 206 IWNDFLLPLLVLQDAELRTIPLATSSFFAQYTKQWDMGLAALVLGITPVIIFFLFLQKHI 265 Query: 267 VEGIAAGSVK 276 ++GIA+GS+K Sbjct: 266 IKGIASGSIK 275 >C4L2X2.1 C4L2X2_EXISA Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 288 bits (736), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 138/268 (51%), Positives = 196/268 (73%), Gaps = 1/268 (0%) Query: 10 NIGI-EVLMILLALLFLSPFYFLLANSVKSFGEILSNAASWPQEFMWSNYTNAWKLARFS 68 N+GI E++ ILL LL+L PFY+++ANS KS EI +N ++ P E++ SNYT AW+ + Sbjct: 33 NLGIIELVAILLGLLYLVPFYYVVANSFKSMAEIFTNTSALPNEWLTSNYTEAWEAMNYG 92 Query: 69 EAFRNXXXXXXXXXXXXXXFSAMAAYRMVRANTRFNQILLLLFVAAMVVPFQTIMIPILK 128 F N F++MAA+++VR + ++ LFVAAMV+PFQ+IMIP++K Sbjct: 93 RVFLNSLLITVGANAVIVIFTSMAAWKLVRTKHWISTMIFFLFVAAMVIPFQSIMIPLVK 152 Query: 129 VVNILGVNNSFAGLIISNLGLSIPMAIFLFHGFIKSVPLEIEEAATVDGCNPISAFFRIV 188 V +ILG+ NS GLI+ LG +++FL+HGF+KS+P EIEEAA +DGC+ F+RIV Sbjct: 153 VSSILGLLNSHWGLIVMYLGFGSGISVFLYHGFMKSIPEEIEEAAIIDGCSTWGVFWRIV 212 Query: 189 LPLLKPMLMTIIVLNALGIWNDYLLPSLILQAPGLRTIPLATFSFFGQYTKQWDMALPAL 248 PLLKP+ +TI++LN+L IWND+LLPSL+L+ LRTIPLATF FFGQYTKQWD+AL L Sbjct: 213 FPLLKPITVTIVILNSLWIWNDFLLPSLVLRDVELRTIPLATFYFFGQYTKQWDLALAGL 272 Query: 249 TLGVAPIVIFYLFMQRYIVEGIAAGSVK 276 LG+ P+++F+ MQ++I++GI +GS+K Sbjct: 273 VLGIVPLLVFFFGMQKHIIKGITSGSIK 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.142 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,435,000,057 Number of extensions: 54346516 Number of successful extensions: 215349 Number of sequences better than 1.0e-05: 6222 Number of HSP's gapped: 208827 Number of HSP's successfully gapped: 6223 Length of query: 277 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 143 Effective length of database: 1,798,280,193 Effective search space: 257154067599 Effective search space used: 257154067599 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)