BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0026.p (393 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0MNI3.1 B0MNI3_9FIRM Putative uncharacterized protein 200 2e-49 C0T5J2.1 C0T5J2_9PSEU Lysophospholipase L1-like esterase 62 7e-08 B2TDP5.1 B2TDP5_BURPP Lipolytic protein G-D-S-L family 61 2e-07 Q023U6.1 Q023U6_SOLUE Lipolytic enzyme, G-D-S-L family 59 1e-06 A9FSD2.1 A9FSD2_SORC5 Family membership 57 4e-06 >B0MNI3.1 B0MNI3_9FIRM Putative uncharacterized protein Length = 446 Score = 200 bits (508), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 133/379 (35%), Positives = 191/379 (50%), Gaps = 31/379 (8%) Query: 16 YTSAMALSTAANYIMNWAEPFTRTYRTYIRLRENGSLTLKFWHSNAVDSTWDLGVEARGS 75 Y S +ST N+ + AE T YR Y+ + E G L KF+ +N VDST+ G A Sbjct: 90 YVSNTVISTGNNFYIEEAENIT--YRAYLPVEEYGELEYKFFFTNTVDSTYAKGKIAYVG 147 Query: 76 EPGGKWSIEAAYIADGGLKPDGTVMQGSQVPVTFLGETAKQVAPGEAFWSDEAALVLPEG 135 + GG +++ A +ADGG + + ++ PVTF GET K V P E++ SD + +PEG Sbjct: 148 KSGGSYTVSNATVADGGTGVEDEIT--NRTPVTFGGETKKDVKPDESYTSDTVTINIPEG 205 Query: 136 HYLAFTWTIRTAAPGKTFPYNVEGMLVSAYDAPGNHAGQETNAAFSESDKLLVLPSYIGY 195 HYL + WT+ GK P M + Q F+ D++ LP IG Sbjct: 206 HYLVWEWTVS----GKGIP--CTKMATLTSTTSSSDGTQ-----FNYCDEIP-LPQLIGA 253 Query: 196 KKKVDKKLVFLGDSITQGVRTAKDEYSYWAARIAEGLGTGYGLWNLGSGWGRAYDAATDG 255 K+ V L +GDSITQG +T +Y +WA++I+ LG+ +N G GW RA DAA + Sbjct: 254 KRNVKHTLAAIGDSITQGCQTEFMKYEFWASKISTQLGSDVAFFNCGLGWARASDAAANE 313 Query: 256 AWLNKAKQGDEVLIVLGVNDLDIG-----KRSAAELLADLAFIVSSIREANPDVEIILGT 310 +WL++ + D V++ G ND+ G K +A E+ L IVS + E DV IL Sbjct: 314 SWLSRVSEYDTVIVAFGTNDIVAGKYGGKKSTAEEIEGCLDTIVSYLTEKGCDV--ILFN 371 Query: 311 VPPFNFEGEREEAWRCVNTQILTAPPAGVNRVFDIAAVLSMPAPLDHKVRAEYMSNTDDP 370 PP +F+ E +N +I + FD +A+LS A + V Sbjct: 372 APPQDFKETNEGIRTALNEKIPAIAEKYEAKFFDFSALLSTEAEPEKAVYG--------G 423 Query: 371 HPNGTAGKAVADAFLKWYG 389 HPNG G VADAF+K +G Sbjct: 424 HPNGEGGTVVADAFVKQFG 442 >C0T5J2.1 C0T5J2_9PSEU Lysophospholipase L1-like esterase Length = 399 Score = 62.4 bits (150), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 90/322 (27%), Positives = 128/322 (39%), Gaps = 49/322 (15%) Query: 90 DGGLKPDGTVMQGSQVPVTFLGETAKQVAPGEAFWSDEAAL-------VLPEGHYLAFTW 142 D G +P G+ VTF G +V G SD L +L H+ T Sbjct: 95 DAGARP----APGTTRAVTFGGSREARVPAGRELLSDPVRLDVAALSTLLVSVHFPGPT- 149 Query: 143 TIRTAAPGKTFPYNVEGMLVSAYDAPGNHAGQETNAAFSESDKLLVLPSYIGYKKKVDKK 202 P P+ +G Y A G+ G T F+++ L + +++ Sbjct: 150 -----GPLTNHPFTAQGN----YLATGDKTGDRTGTGFTDTPCWLGVSGVDVARERAAGT 200 Query: 203 LVFLGDSITQGVRTAKDEYSYW----AARIAEGLGTGYGLWNLGSGWGRA---------Y 249 +V GDSIT T + W A R+AE + N G G R Y Sbjct: 201 VVAFGDSITDTSATTGNTNRRWPDFLARRLAE-RRHAPSVVNAGLGGNRLVADREGEPYY 259 Query: 250 DAATDGAWLNKA--KQGDEVLIVL-GVNDLDIGKRSAAELLADLAFIVSSIREANPDVEI 306 A + A + G ++VL GVND+ G +A EL+A +++ V I Sbjct: 260 GVAGTTRFARDALGQTGVRTVVVLEGVNDIGFGA-TADELVAGFRNLIAQAHARG--VRI 316 Query: 307 ILGTVPPFN----FEGEREEAWRCVNTQILTAPPAGVNRVFDIAAVLSMPAPLDHKVRAE 362 + GT+ PF + ERE WR VNT I T+ + V D AAV + AP D V A Sbjct: 317 VGGTILPFKTSFVWTPEREATWRAVNTWIRTS--GEFDAVIDFAAVTA--APDDDAVLAP 372 Query: 363 YMSNTDDPHPNGTAGKAVADAF 384 + D HP +A+ADA Sbjct: 373 AYDSGDGLHPGDAGTEAMADAI 394 >B2TDP5.1 B2TDP5_BURPP Lipolytic protein G-D-S-L family Length = 407 Score = 61.2 bits (147), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 90/327 (27%), Positives = 139/327 (42%), Gaps = 28/327 (8%) Query: 74 GSEPGGKWSIEAAYIADGGLKPDGTVMQGSQVPVTFLGETAKQVAPGEAFWSDEAALVLP 133 GSEP I A IA G P + GS VTF G+ +A G + SD A +P Sbjct: 79 GSEP---LVIGEARIARGA--PGQQTVPGSDRAVTFGGQPNVTIAVGASVVSDPVAFEVP 133 Query: 134 EGHYLAFTW--TIRTAAPGKTFPYNVEGMLVSAYDAPGNHAGQETNAAFSESDKLLVLPS 191 +A + +RTA ++ + ++A D + A + L + Sbjct: 134 ALSDIAISLYLPVRTAPRSTGHVDGLQDIYIAAGDVAADPAFTGGSTFSPGGQSYYFLTN 193 Query: 192 YIGYKKKVDKKLVFLGDSITQGVRTAKDEYSYWAARIAEGL---GTGYGLWNLGSGWGRA 248 +V G SIT G++++ + W R+A L G G+ N G Sbjct: 194 LDVMSPAATGAVVTFGASITDGLKSSVNGNHRWPNRLAIRLQQAGMTVGVLNQGISGNDF 253 Query: 249 YDAATDGAWLNKAKQGDEVL-------IVL---GVNDLDIGKRS-AAELLADLAFIVSSI 297 + A L + G +VL +V+ +NDL+ K S AA L+ L ++ Sbjct: 254 FTNNAGQAGLKRF--GRDVLRQPGANWVVISDDAINDLNTDKPSTAARLIGALRQLIERA 311 Query: 298 REANPDVEIILGTVPPFNFEGEREEAWRCVNTQILTAPPAGVNRVFDIAAVLSMPAPLDH 357 AN +++I T+ PF G E A + +N IL++ +G + V D A +S PA + Sbjct: 312 HRAN--IKVICSTLTPFAATGPVEAARQDINAFILSST-SGCDAVLDQAKAVSDPANPAN 368 Query: 358 KVRAEYMSNTDDPHPNGTAGKAVADAF 384 + A Y S DD HPN +A+ADA Sbjct: 369 LLPA-YNSG-DDLHPNEAGLQAIADAM 393 >Q023U6.1 Q023U6_SOLUE Lipolytic enzyme, G-D-S-L family Length = 417 Score = 58.5 bits (140), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 89/329 (27%), Positives = 146/329 (44%), Gaps = 45/329 (13%) Query: 82 SIEAAYIADGGLKPDGTVMQGSQVPVTFLGETAKQVAPGEAFWSDEAALVLPEGHYLAFT 141 ++ AA+IA + D ++ S ++F G+ + V PG +SD + +P LA + Sbjct: 100 TVGAAHIALRAKESD--IVAASDHALSFNGKPSCTVGPGMVIFSDALDMTVPGQSDLAVS 157 Query: 142 WTIRTAAPGKTFPYNVEGMLVSAYDAPGNHAGQETNAAFSESDKLLVLPSY-----IGYK 196 + PG+T P + L ++Y G+ Q + A + + P+Y I Sbjct: 158 LYL----PGETGPPSAHNGLHTSYVKEGD---QTSAPALPDP---IAAPNYYWLAGIDVL 207 Query: 197 KKVDKKL-VFLGDSITQGVRTAKDEYSYW----AARIAEGLGTGY-GLWNLGSGWGRAYD 250 D L V GDSIT+G R+ D W +AR+A GT + + N+G G R Sbjct: 208 APADSSLLVTYGDSITEGARSTPDTDRMWPAVLSARLAANKGTAHIAVANMGIGGNRVLR 267 Query: 251 AATDGAWLNK------AKQGDE-VLIVLGVNDLDIGKR---SAAELLADLAFIVSSIREA 300 + + L + ++ G + V+++ G+ND+ G ++ +L+ I+ R Sbjct: 268 DGSGASALARFDRDVLSQSGVKWVMMLEGINDIGHGDTEPITSDDLIGAYRQIIE--RAH 325 Query: 301 NPDVEIILGTVPPFNFEG---EREEAWR-CVNTQILTAPPAGVNRVFDI-AAVLSMPAPL 355 + +I T+PP+ G E EA R +NT I T + V D AA P Sbjct: 326 THGIRVIGCTLPPYEGAGYSREEGEAIRQALNTWIRTG--KAFDAVVDFEAATRDSNNP- 382 Query: 356 DHKVRAEYMSNTDDPHPNGTAGKAVADAF 384 K+RA++ D HPN KA+ADA Sbjct: 383 -RKLRADF-DPGDHLHPNDAGYKAMADAV 409 >A9FSD2.1 A9FSD2_SORC5 Family membership Length = 453 Score = 56.6 bits (135), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 103/382 (26%), Positives = 154/382 (40%), Gaps = 70/382 (18%) Query: 38 RTYRTYIRLRENGSLTLKFWHSNAVDSTWDLGVEARGSEPGGKWSIEAAYIADGGLKPDG 97 +T R +R+ GS + + SNA GSEP S A A G Sbjct: 92 QTVRNIVRVSAGGS-SARVRISNA-----------GGSEPLKVGSASVALSAGGA----- 134 Query: 98 TVMQGSQVPVTFLGETAKQVAPGEAFWSDEAALVLPEGHYLAFTWTIRTAAPGKTFPYNV 157 + GS +P+ F G+T+ +A G SD AL + LA + PGKT P + Sbjct: 135 ATVAGSSLPLRFAGQTSIVIAAGGEAVSDPVALKVQPLQTLAVNVYL----PGKTGP--I 188 Query: 158 EGMLVSAYD---APGNHAGQETNAAFSESDKLLVLPSYIGYKKKVD--KKLVFLGDSITQ 212 +A D A GN G + F+ + S + K D L+ LGDSIT Sbjct: 189 TQHYFAAQDNFLASGNQTGNGADKQFTRKISCWMAVSGVDVKASPDVVGTLITLGDSITD 248 Query: 213 GVRTAKDEY----SYWAARIAEGLGTGYGLWNLGS--------------GWGRAYDAATD 254 G + K++ Y A R+A G + N G G+ A D Sbjct: 249 GYLSTKNDNHRYPDYLAQRLASRKGPTLAVANAGIIGNELLTVRPQLQFGYPVPARLARD 308 Query: 255 GAWLNKAKQGDEVLIVLGVNDLDIGKRS--AAELLADLAFIVSSIREANPDVEIILGTVP 312 A+ ++++ G+N DIG RS AA+L+ + I+ S R A ++I GT+ Sbjct: 309 VLTQPGAR---AIILLAGIN--DIGDRSAKAADLIPAIQQIIQSARAAG--LKIYGGTLV 361 Query: 313 PF-----NFEGER-----EEAWRCVNTQILTAPPAGVNRVFDIAAVLSMPAPLDHKVRAE 362 PF ++G+ E+ + +NT I T+ + V D L PA R Sbjct: 362 PFAGSNGTYKGDYGTAKGEQERQKLNTWIRTS--GAFDGVIDFDKALRDPA---DPSRLL 416 Query: 363 YMSNTDDPHPNGTAGKAVADAF 384 + D HPN +A+A+A Sbjct: 417 PAYDADKLHPNDAGYQAMANAV 438 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,522,247,282 Number of extensions: 110979721 Number of successful extensions: 253728 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 253728 Number of HSP's successfully gapped: 40 Length of query: 393 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 255 Effective length of database: 1,760,596,133 Effective search space: 448952013915 Effective search space used: 448952013915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)