BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0017.p (389 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4CLL1.1 C4CLL1_9CHLR Predicted TIM-barrel fold metal-dependent ... 219 3e-55 B8HRT9.1 B8HRT9_CYAP4 Amidohydrolase 2 174 1e-41 B4VKN3.1 B4VKN3_9CYAN Amidohydrolase family 174 1e-41 Q4J6Z5.1 Q4J6Z5_SULAC Conserved protein 172 7e-41 B4B0A4.1 B4B0A4_9CHRO Amidohydrolase 2 171 9e-41 >C4CLL1.1 C4CLL1_9CHLR Predicted TIM-barrel fold metal-dependent hydrolase, COG2159 Length = 392 Score = 219 bits (559), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 129/403 (32%), Positives = 217/403 (53%), Gaps = 41/403 (10%) Query: 3 IDISRLPIIDDHCHPFM---PTREKKSFD----------------NCFTLSLIPPKSRDT 43 +D+++LP++D HCHPF+ P ++ D T ++ R Sbjct: 2 LDLTQLPVVDVHCHPFLNRGPVDAEQFTDLTAFGGGSRQYLEEGGVAVTDAVHAELQRVK 61 Query: 44 RNTLLFKIVRKEMIQLLGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDY 103 R TL F+ + +++ G P D ++ RN+ + + YV L+ + GL +L+ D+ Sbjct: 62 RYTLYFRRMLRDLAAFFGCAPDL--DTVLAHRNRAVEEDYTSYVRRLYAEAGLTTLVADF 119 Query: 104 GFPVMGSRLAQDELDWFHQTSSSIPTR--RIHRVEATYHRLFEEKVPFGELEETYIRELR 161 GFP+ LD + +P I R+E L +P+ E + Y + Sbjct: 120 GFPL-------PMLD-IEAVRAELPVEVVPIFRIEPLIVDLLAMDLPWAEFRQRYDDTIA 171 Query: 162 DEVENQGTIAFKTVIAYHTGLKINKISKS-----QAAASYSRFLESPDNTEAEKDVRDYL 216 D ++++G K++IAY TGL I+ +S++ QA + R L + K +RD+L Sbjct: 172 DALQHKGYKGVKSIIAYRTGLDISPLSRTPDQGYQALDAIRRGL----GGGSMKKLRDHL 227 Query: 217 VLLAFELCMEYDIPIQMHSGAGDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPF 276 + A ELC+EYD+P+Q+H+G GD + ++ P + ++ P Y+A +++ +HT YP+ Sbjct: 228 LCRALELCIEYDVPMQIHTGMGDFEV-NLVYCRPAYLMELLRFPAYRACRVLLVHTGYPY 286 Query: 277 VEEAGYLVNQYHNVYLDLSSMIPFSSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWL 336 EA Y+ N VY D+S IPF+ + L ++ EMAP +KV+YGSDG+ +PE+++ Sbjct: 287 HGEAAYMANVLPRVYCDVSEGIPFAGHAARRILSEVLEMAPISKVVYGSDGYALPEINYT 346 Query: 337 GGRMIKKELGKMLTDLVTDGIIDEEYAYTAAGLVLSENARNLY 379 ++ K+ LG++L+DLVTD ++ E A AAGL+LS NAR LY Sbjct: 347 SAKLGKQALGQVLSDLVTDNMLSEAEAQEAAGLILSGNARELY 389 >B8HRT9.1 B8HRT9_CYAP4 Amidohydrolase 2 Length = 375 Score = 174 bits (442), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 121/383 (31%), Positives = 193/383 (50%), Gaps = 21/383 (5%) Query: 3 IDISRLPIIDDHCHPFMP--TREKKSFDNCFTLSLIPPK-SRDTRNTLLFKIVRKEMIQL 59 +D+ + +ID H H + E+ + FT + P TR++L + +E+ L Sbjct: 1 MDLGGIALIDQHAHNLLKPEVAERLPYPAAFTEAHNPTLWHHHTRHSLTYHRSLREIATL 60 Query: 60 LGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDYGFPVMGSRLAQDELDW 119 L D T+ ++ QR + + F LE +L+D GF + G L L+W Sbjct: 61 LQCD--LTEAAILAQRQT---LGLEALTRLCFSQSHLEGILLDDGF-LPGDIL---PLEW 111 Query: 120 FHQTSSSIPTRRIHRVEATYHRLFEEKVPFGELEETYIRELRDEVENQGTIAFKTVIAYH 179 Q +P R+ R+E+ LF + F + + + + L E G + FKT+ AY Sbjct: 112 HRQF---LPVHRLLRLESLAEELFWQSANFEQFHQQFRQVL--EQPPPGVVGFKTIAAYR 166 Query: 180 TGLKINKISKSQAAASYSRFLESPDNTEAEKD--VRDYLVLLAFELCMEYDIPIQMHSGA 237 TGL I ++ +AAAS++R S N +D + ++L++ A E+ ++P+Q H+G Sbjct: 167 TGLDIRFVTPEEAAASFNRLRLSDQNPLRLQDPILINFLLVQALEIAARQELPVQFHTGF 226 Query: 238 GDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFVEEAGYLVNQYHNVYLDLSSM 297 GD P LD++LA+PL + ++ PH QA I+ +H AYP+ EAGYL Y VY+D Sbjct: 227 GD-PDLDLRLANPLHLRPLLEAPHLQAAPIVLLHAAYPYSREAGYLAAVYPQVYVDFGLA 285 Query: 298 IP-FSSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLGGRMIKKELGKMLTDLVTDG 356 IP S G+Q + + E AP +K++Y SD IPE+ +L R ++ LG +L + G Sbjct: 286 IPLLSRAGMQQTITMLLEFAPASKLLYSSDAHFIPELYYLAARWGRQSLGAVLEQSIRAG 345 Query: 357 IIDEEYAYTAAGLVLSENARNLY 379 I A A +L NA LY Sbjct: 346 EITASIAEAMALAILRGNALRLY 368 >B4VKN3.1 B4VKN3_9CYAN Amidohydrolase family Length = 373 Score = 174 bits (441), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 120/389 (30%), Positives = 209/389 (53%), Gaps = 31/389 (7%) Query: 3 IDISRLPIIDDHCHPFM-PTREKK-SFDNCFTLSLIPPK-SRDTRNTLLFKIVRKEMIQL 59 +++ + ID H H + P+ K+ + FT P + R +L ++ +++ QL Sbjct: 2 LNLWDIAAIDQHAHNLLNPSATKRYPYTAAFTEGYHPDIINHHARYSLFYRRSLRDIAQL 61 Query: 60 LGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDYGFPVMGSRLAQDELDW 119 L +P + +IL+ R Q ++ F LE++ +D GF + G + L+W Sbjct: 62 LDCEP---TEAVILE--HRRQLGLEKLTQKCFHASNLEAIFLDDGF-LPGDIMP---LEW 112 Query: 120 FHQTSSSIPTRRIHRVEATYHRLFEEKVPFGELEETYIRELRDEVE--NQGTIAFKTVIA 177 Q +P +RI R+E +L E F +T+ + R +++ + G + FK+V A Sbjct: 113 HQQF---VPVQRILRLEYLAEKLIIETDDF----KTFWEQFRSQIDPPSAGIVGFKSVAA 165 Query: 178 YHTGLKINKISKSQAAASYSRFLE-SPDNTEA----EKDVRDYLVLLAFELCMEYDIPIQ 232 Y TGL I K A RF E P ++++ +K D+++ A E+ +Y +PIQ Sbjct: 166 YRTGLDIQWTPKEFAK---QRFYEIKPTSSQSLRLSDKPFIDFVITQALEIASKYQLPIQ 222 Query: 233 MHSGAGDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFVEEAGYLVNQYHNVYL 292 H+G GD P LD++L++PL + S++ D ++ V I+ +H AYP+ +EAGYL + Y VYL Sbjct: 223 FHTGFGD-PDLDLRLSNPLYLRSLLEDKRFENVPIVLLHAAYPYSQEAGYLASVYPQVYL 281 Query: 293 DLSSMIPF-SSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLGGRMIKKELGKMLTD 351 D +PF S G++ ++Q+ E+ P +K+MY SD IPE+ +LG + ++ L ++L Sbjct: 282 DFGLAVPFLSHAGMRRTVQQLLELTPTSKLMYSSDAHNIPELYYLGAKWGREVLSQVLDG 341 Query: 352 LVTDGIIDEEYAYTAAGLVLSENARNLYI 380 + DG + + A + A +L +NAR LY+ Sbjct: 342 AIHDGDLTAKEAESVAYAILCDNARKLYL 370 >Q4J6Z5.1 Q4J6Z5_SULAC Conserved protein Length = 336 Score = 172 bits (435), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 109/335 (32%), Positives = 184/335 (54%), Gaps = 36/335 (10%) Query: 48 LFKIVRKEMIQLLGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDYGFPV 107 + +++KEM +LLG D+ I +RN+ + +P Y+ L D +++ ++D GF Sbjct: 32 FYLMLKKEMKRLLG-------DDFIKERNRLIRDDPVSYMKFLIDDAKIKAFVIDEGF-- 82 Query: 108 MGSRLAQDELDWFHQTSSSIPTRRIHRVEATYHR-LFEEKVPFGELEETYIRELRDEVEN 166 GS+ + P +R+ R+E+ + LF +PF + E + LR++V Sbjct: 83 -GSK----------EMEIPAPYKRLFRIESIINSSLFS--LPFDKAIEFFEETLRNKV-R 128 Query: 167 QGTIAFKTVIAYHTGLKINKISKSQAAASYSRFLESPDN--TEAEKDVRDYLVLLAFELC 224 +G + FK++IAY TGL I + FL ++ E +K RDYL+ E+ Sbjct: 129 EGYVGFKSIIAYRTGLNIT----CNELKALEDFLSRDEDWYGEKKKGFRDYLLCKTMEIA 184 Query: 225 MEYDIPIQMHSGAGDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFVEEAGYLV 284 E D+P+Q+H+GAGD + ++L+ P + ++V Y+ KI+F+H+ YP+ E ++ Sbjct: 185 KELDVPLQVHTGAGDRDI-KLELSRPAYLTNVVR--RYEG-KIVFVHSGYPYHRETAWMS 240 Query: 285 NQYHNVYLDLSSMIPFSSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLGGRMIKKE 344 + +VYLDLS + PF+ +G L +I E+APF K+MYGSDGF IPE++W+ ++ + Sbjct: 241 YIFPSVYLDLSQVCPFAPLGALNALEEIMEVAPFNKIMYGSDGFNIPEIAWISAKIFPRY 300 Query: 345 LGKMLTDLVTDGIIDEEYAYTAAGLVLSENARNLY 379 L +L +V+ GI E ++LS NA LY Sbjct: 301 LDMVLNKMVSLGI--IEEEEEIRQMILSRNASRLY 333 >B4B0A4.1 B4B0A4_9CHRO Amidohydrolase 2 Length = 371 Score = 171 bits (434), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 114/379 (30%), Positives = 199/379 (52%), Gaps = 28/379 (7%) Query: 11 IDDHCHPFMPTR--EKKSFDNCFTLSLIPPK-SRDTRNTLLFKIVRKEMIQLLGLDPRAT 67 ID H H + +K ++ FT P + R+TL + +E+ QLL + Sbjct: 7 IDHHAHNLLKPEIAQKYAYAAFFTEGYDPEIINHHARHTLFYYRSLREIAQLLNCE---A 63 Query: 68 DDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDYGFPVMGSRLAQDELDW-FHQTSSS 126 + IL+R Q + ++ D FK LE++ +D GF + W +HQ + Sbjct: 64 TEAAILERRQ--ELGLEKLADCCFKAANLEAIFLDDGF------FPSEVFPWQWHQ--NF 113 Query: 127 IPTRRIHRVEATYHRLFEEKVPFGELEETYIRELRDEVEN--QGTIAFKTVIAYHTGLKI 184 + +R+ R+E L E+ +P E + ++ R E++ + +AFK++++Y TGL I Sbjct: 114 VKVKRVLRLE----YLAEQLIPRAENCKNFLEAFRSEIDPPPREVVAFKSIVSYRTGLNI 169 Query: 185 NKISKSQAAASYSRFLESPDNTEAE---KDVRDYLVLLAFELCMEYDIPIQMHSGAGDAP 241 + +S A +S+ + ++ K + D+L++ A E+ +Y IPIQ H+G GD P Sbjct: 170 DLVSLEVAESSFEQVKQACQEKPCRLNNKHLIDFLLIQALEIANKYRIPIQFHTGFGD-P 228 Query: 242 LLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFVEEAGYLVNQYHNVYLDLSSMIPFS 301 LD+KL++PL + ++ D +Q II +H +YP+ E GYL + Y +YLD +PF Sbjct: 229 DLDLKLSNPLYLRPLLEDKRWQNAPIILLHASYPYTREGGYLASVYPQIYLDTGLAVPFL 288 Query: 302 SI-GVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLGGRMIKKELGKMLTDLVTDGIIDE 360 S+ G++ ++Q+ E+ P TK+MY SD IPE+ +L + +K L +L + D + Sbjct: 289 SVAGMRSCVQQLLELTPTTKLMYSSDAHHIPELYYLAAKWGRKILADVLEAAIKDTDLTA 348 Query: 361 EYAYTAAGLVLSENARNLY 379 + + +LSENAR++Y Sbjct: 349 KQVDLISKAILSENARHVY 367 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,325,490,382 Number of extensions: 95976844 Number of successful extensions: 207442 Number of sequences better than 1.0e-05: 115 Number of HSP's gapped: 207191 Number of HSP's successfully gapped: 115 Length of query: 389 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 251 Effective length of database: 1,760,596,133 Effective search space: 441909629383 Effective search space used: 441909629383 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)