BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0011.p (462 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D4S6.1 B1D4S6_9BACL Threonine synthase EC=4.2.3.1 678 0.0 B9M1F1.1 B9M1F1_GEOSF Threonine synthase EC=4.2.3.1 483 e-134 B3E4R0.1 B3E4R0_GEOLS Threonine synthase EC=4.2.3.1 482 e-134 A5GF77.1 A5GF77_GEOUR L-threonine synthase EC=4.2.3.1 481 e-134 Q39V62.1 Q39V62_GEOMG L-threonine synthase EC=4.2.3.1 481 e-134 >B1D4S6.1 B1D4S6_9BACL Threonine synthase EC=4.2.3.1 Length = 466 Score = 678 bits (1749), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/464 (68%), Positives = 385/464 (82%), Gaps = 3/464 (0%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 MEYISTRGNV GF+D LMGLADDGGL+VP+ IPV+S KL EW+ LS+QEL LEIFS Sbjct: 1 MEYISTRGNVGKIGFVDAFLMGLADDGGLLVPYEIPVVSAAKLKEWQELSYQELVLEIFS 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 YTNDEIPYEDLK+MVY YA FR PEVTP+H++ D LY+LELFHGPTFAFKD+ALQFMG Sbjct: 61 LYTNDEIPYEDLKKMVYDGYATFRHPEVTPVHKLKDDLYLLELFHGPTFAFKDIALQFMG 120 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 +LY+Y+SRK+ + IHILGATSGDTGA+AI+GV+GKEGIKICILHP+GKVSKVQELQMTT+ Sbjct: 121 QLYTYVSRKQNKKIHILGATSGDTGASAIEGVKGKEGIKICILHPNGKVSKVQELQMTTI 180 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D NVLNLSVEGNFDDCQ++IK+LFADL+FK HL AINSINFVRI+AQTVYYFY+Y Q Sbjct: 181 LDDNVLNLSVEGNFDDCQRIIKDLFADLEFKAANHLCAINSINFVRIMAQTVYYFYSYFQ 240 Query: 241 IEDSDKK---KINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYK 297 + K K+N SVP+GNFG+IF+GYLAK+MGLP+ KLI+ATNENNILERF+ G YK Sbjct: 241 VAKQTGKQDAKVNFSVPTGNFGDIFAGYLAKRMGLPVDKLILATNENNILERFIKEGVYK 300 Query: 298 PGSFTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQA 357 PG F +TYSPSMDIQVASNFERYLYY++ E+ E++ + M ++EG+I ++G+AL++VQ Sbjct: 301 PGDFRTTYSPSMDIQVASNFERYLYYVLDENPEQIRSIMDGFKSEGQIVVSGDALRKVQE 360 Query: 358 DFAALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFD 417 +FAA GV+ ++CLDTI YNKE YLLDPH+ACG+AA ++ AG I+ ATAHPAKF+ Sbjct: 361 EFAAHGVEGRECLDTIAAYNKETGYLLDPHSACGVAASDQQAAAGSVTISLATAHPAKFN 420 Query: 418 EAIALVDIRQEFPEQIAALFSLPQHQTVVKNDKDVIVRELQAFY 461 EAI L+ I QEFP QI ALF+ PQ QT V ++ IV+E+ FY Sbjct: 421 EAIELIGIEQEFPAQIQALFNKPQRQTQVDGTQEAIVQEIVKFY 464 >B9M1F1.1 B9M1F1_GEOSF Threonine synthase EC=4.2.3.1 Length = 460 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 235/454 (51%), Positives = 313/454 (68%), Gaps = 4/454 (0%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M+YISTRGN+ GF D V+MGLA DGGL++P R PVI L+ WRSL ++EL ++ S Sbjct: 1 MKYISTRGNIAPVGFKDAVMMGLATDGGLLLPERFPVIDDGTLSAWRSLPYRELAFQVIS 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 + D+IP DLK ++ SY +F E+TP+ N +++LELFHGPT AFKDVALQ +G Sbjct: 61 RFV-DDIPAADLKNLIDKSYDSFAHEEITPVVAKN-GVHILELFHGPTLAFKDVALQLLG 118 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+ Y+ ++ G ++I+GATSGDTG+AAI GVRGKE I I ILHP+GK S +Q QMTTV Sbjct: 119 NLFEYLLKESGRKMNIVGATSGDTGSAAIYGVRGKENINIFILHPYGKTSPIQARQMTTV 178 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D+NV N++V G FDDCQ ++K+LF DL FK +Y L A+NSIN+ R+LAQ VYYFYA+ + Sbjct: 179 LDANVFNIAVNGTFDDCQNIVKDLFNDLPFKEQYALGAVNSINWARVLAQVVYYFYAWFR 238 Query: 241 IEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGS 300 + DS + + SVP+GNFG+IF+GY+AK+MGLPI KL++ATNENNIL RF+N G+Y GS Sbjct: 239 VTDSQESPVVFSVPTGNFGDIFAGYVAKRMGLPIAKLLLATNENNILTRFINDGDYSKGS 298 Query: 301 FTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFA 360 T SPSMDIQVASNFERYLYYL E ++ S+ G++T E L++V+ DFA Sbjct: 299 VVQTVSPSMDIQVASNFERYLYYLFDESPARVRECFSQFAATGRMTFTAEELERVRKDFA 358 Query: 361 ALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEAI 420 + V ++ L+TI ++ E Y+LDPHTA G+ A + AG T + ATAHPAKF EA+ Sbjct: 359 SRSVNEEETLETIAAFHLETSYILDPHTAVGVKAAQDC-AAGSTVVCLATAHPAKFAEAV 417 Query: 421 -ALVDIRQEFPEQIAALFSLPQHQTVVKNDKDVI 453 R P + + LP V+ D+ I Sbjct: 418 EKATGNRPPLPGSLEGIEQLPTRCEVMPADRATI 451 >B3E4R0.1 B3E4R0_GEOLS Threonine synthase EC=4.2.3.1 Length = 460 Score = 482 bits (1240), Expect = e-134, Method: Compositional matrix adjust. Identities = 237/450 (52%), Positives = 313/450 (69%), Gaps = 4/450 (0%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M Y+STRG + F D V+MGLADDGGL++P IP S +L WR+L++ +L +I S Sbjct: 1 MRYLSTRGGIAPVRFKDAVMMGLADDGGLLLPESIPAFSAAELESWRTLTYPQLAFKIIS 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 + +D IP DL+ ++ SYA F PEVTP+ Q D +Y+LELFHG T AFKDVALQF+G Sbjct: 61 RFVDD-IPATDLQALIDRSYATFSHPEVTPVVQ-KDGVYILELFHGVTLAFKDVALQFLG 118 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+ YI +R E + I+GATSGDTG+AAI GVRGK GI I ILHPHGK S VQ LQMT+V Sbjct: 119 NLFEYILHERHERLTIVGATSGDTGSAAIHGVRGKAGISIFILHPHGKTSPVQALQMTSV 178 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D NV N++V+G FDDCQ ++K L DL+FK KY L A+NSIN+ R+LAQ VYY YA+L+ Sbjct: 179 LDENVHNIAVDGTFDDCQDIVKGLMGDLEFKQKYSLGAVNSINWARVLAQVVYYIYAWLR 238 Query: 241 IEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGS 300 + + +K + SVP+GNFG+IF+GY+A++MGLP+ KL++ATNENNIL RF+N G+Y G+ Sbjct: 239 VAEQGRKVV-FSVPTGNFGDIFAGYMAQQMGLPVEKLLLATNENNILTRFINQGDYSVGT 297 Query: 301 FTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFA 360 T SPSMDIQVASNFERYL+YL +DA ++SA ++L+ EGKI+ + + LQQV+ DF Sbjct: 298 VVQTLSPSMDIQVASNFERYLFYLFKQDAARVSAAFAQLKAEGKISFSQQELQQVRQDFR 357 Query: 361 ALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEAI 420 + V Q L+TI ++ + YLLDPHTA G+ A ++ + ATAHPAKF EA+ Sbjct: 358 SASVDRQATLETIRQFKHDTGYLLDPHTAVGVRAAQELVTDDTPVVCLATAHPAKFGEAV 417 Query: 421 A-LVDIRQEFPEQIAALFSLPQHQTVVKND 449 P IA L LP ++ D Sbjct: 418 VEATGDAVPVPPAIAQLEGLPTRCVRMQAD 447 >A5GF77.1 A5GF77_GEOUR L-threonine synthase EC=4.2.3.1 Length = 460 Score = 481 bits (1239), Expect = e-134, Method: Compositional matrix adjust. Identities = 235/454 (51%), Positives = 315/454 (69%), Gaps = 4/454 (0%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M+YISTRGN++ GF + V+MGLA DGGL++P RIP I L+ WR L ++EL I S Sbjct: 1 MKYISTRGNIQPVGFKEAVMMGLATDGGLLLPERIPAIDAATLSSWRQLPYRELAFRIIS 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 + D+IP DLK ++ +SY +F E+TP+ + N +Y+ ELFHGPT AFKDVALQF+G Sbjct: 61 LFA-DDIPANDLKRLIDSSYDSFDHKEITPVVKEN-GVYIAELFHGPTLAFKDVALQFLG 118 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+ Y+ ++ G+ ++I+GATSGDTG+AAI GVRGKE I I ILHPHGK S +Q QMTTV Sbjct: 119 NLFEYLLKESGQKMNIVGATSGDTGSAAIYGVRGKENINIFILHPHGKTSPIQARQMTTV 178 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D+NV N++V+G FDDCQ ++KELF DL FK KY L A+NSIN+ R+LAQ VYYFYA+ + Sbjct: 179 LDANVHNIAVKGTFDDCQNIVKELFNDLTFKEKYALGAVNSINWARVLAQVVYYFYAWFR 238 Query: 241 IEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGS 300 + D I SVP+GNFG+IF+G++AK+MGLPI KL++ATNENNIL RF+N G+Y GS Sbjct: 239 VSDEVDSPITFSVPTGNFGDIFAGFVAKRMGLPIDKLLLATNENNILTRFINEGDYSIGS 298 Query: 301 FTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFA 360 T SPSMDIQVASNFERYLYYL E+ ++ ++ G++ E ++ V+ +FA Sbjct: 299 VVQTVSPSMDIQVASNFERYLYYLFDENPARVRDIFTRFAATGRMDFTDEEMRTVRREFA 358 Query: 361 ALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEAI 420 + V Q+ LDTI +++E YLLDPHTA G+ A G TA+ ATAHPAKF EA+ Sbjct: 359 SRSVNEQETLDTIAAFHQETGYLLDPHTAVGVKAALACVTDG-TAVCLATAHPAKFGEAV 417 Query: 421 -ALVDIRQEFPEQIAALFSLPQHQTVVKNDKDVI 453 FP +A + LP +++ ++ I Sbjct: 418 EKATGTAPPFPACLAGIEQLPTRCEIMEANRTAI 451 >Q39V62.1 Q39V62_GEOMG L-threonine synthase EC=4.2.3.1 Length = 461 Score = 481 bits (1238), Expect = e-134, Method: Compositional matrix adjust. Identities = 235/463 (50%), Positives = 318/463 (68%), Gaps = 7/463 (1%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M YISTRG + GF D V+MGLA DGGL++P IP + L W+SLS+Q+L + S Sbjct: 1 MNYISTRGAIAPIGFKDAVMMGLATDGGLLLPETIPALGRNTLESWQSLSYQDLAFNVIS 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 + D+IP +DLK+++ SYA F PE+TP+ + D +Y+LELFHGPT AFKDVALQ +G Sbjct: 61 LFA-DDIPAQDLKDLIDRSYATFSHPEITPVVE-KDGVYILELFHGPTLAFKDVALQLLG 118 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+ Y+ ++RGE ++I+GATSGDTG+AAI GVRGK+ I I ILHPHGK S VQ LQMTTV Sbjct: 119 NLFEYLLKERGEKMNIVGATSGDTGSAAIYGVRGKDKINIFILHPHGKTSPVQALQMTTV 178 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D NV N++ G FDDCQ ++K LF+DL FK KY L A+NSIN+ R+LAQ VYYFYAY + Sbjct: 179 LDPNVHNIAARGTFDDCQNIVKSLFSDLPFKEKYSLGAVNSINWARVLAQVVYYFYAYFR 238 Query: 241 IEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGS 300 + +++ SVP+GNFG+IF+GY+AK+MGLPI +LI+ATNENNIL RF+N G+Y G Sbjct: 239 VAALFGQEVVFSVPTGNFGDIFAGYVAKRMGLPIRRLILATNENNILSRFINGGDYSLGD 298 Query: 301 FTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFA 360 +T SPSMDIQ+ASNFERY+YYL GE+ ++ + L T+G+I +++V+ +F Sbjct: 299 VVATVSPSMDIQLASNFERYVYYLFGENPARVREAFAALPTKGRIDFTEAEMEKVRDEFL 358 Query: 361 ALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEAI 420 + V + + TI +++E Y+LDPHTA G+ A + G A+ ATAHPAKF EA+ Sbjct: 359 SRSVNEDETIATIAAFHRETGYILDPHTAVGVKAALELVQDGTPAVCLATAHPAKFAEAV 418 Query: 421 A-LVDIRQEFPEQIAALFSLPQHQTVVKNDKDVIVRELQAFYE 462 V P + + +LP V+ D+D I +AF E Sbjct: 419 VRAVGFEPSRPTSLEGIEALPSRCDVLDADRDAI----KAFIE 457 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,759,517,795 Number of extensions: 118432861 Number of successful extensions: 289637 Number of sequences better than 1.0e-05: 1143 Number of HSP's gapped: 285573 Number of HSP's successfully gapped: 1150 Length of query: 462 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 323 Effective length of database: 1,751,175,118 Effective search space: 565629563114 Effective search space used: 565629563114 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)