BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0004.p (320 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z8N9.1 C0Z8N9_BREBN Putative nucleosidase EC=3.2.2.1 407 e-112 A8FJ31.1 A8FJ31_BACP2 Purine nucleosidase EC=3.2.2.1 380 e-104 B4AJZ7.1 B4AJZ7_BACPU Purine nucleosidase 362 3e-98 Q5WD21.1 Q5WD21_BACSK Inosine-uridine preferring nucleoside hydr... 336 2e-90 C0Z920.1 C0Z920_BREBN Putative nucleosidase 226 2e-57 >C0Z8N9.1 C0Z8N9_BREBN Putative nucleosidase EC=3.2.2.1 Length = 309 Score = 407 bits (1046), Expect = e-112, Method: Compositional matrix adjust. Identities = 199/307 (64%), Positives = 239/307 (77%) Query: 5 KKVILDVDTGVDDALAIMLAVTSGRFDILGITTVSGNVSLDQATLNTCKILELLGVSGQI 64 KK+ILDVDTG+DDAL I+LAV SG+FD++GITTV+GNVSL +AT NTCKIL+ L V QI Sbjct: 2 KKIILDVDTGIDDALGIILAVKSGQFDLVGITTVNGNVSLRKATENTCKILDFLQVGEQI 61 Query: 65 PVYRGADKPLVREAVFEHRVHGSDGIGGALGDMPVTKQPEAEAAADFIIRQVLAQPGEVT 124 PV GA KPL+R FEH VHG DG+GGAL +P+ K+P A A DFI+ VLA GE+T Sbjct: 62 PVICGASKPLLRPLFFEHAVHGEDGLGGALAGVPIYKEPTAGFAPDFIVENVLAHSGEIT 121 Query: 125 LIMTAPLTNLANALQKCPELVQHAAEVIVMGGVVQGYGNITPTAEYNMYVDPEAAKQVLA 184 L+MT PLTNLA A++K PELVQH EVI MGGVV+ +GN+TP AEYNMYVDPEAAK V Sbjct: 122 LVMTGPLTNLALAVKKYPELVQHVKEVIFMGGVVREHGNVTPVAEYNMYVDPEAAKVVFH 181 Query: 185 AGFPRLTLVGLDVTRRALLGAEDIQKLHNPVIREYVETSTAGYRARYYERNGVQACALHD 244 AGFP+LT+VGLDVTRR LL E I+ L + + EYV ST+ Y RY++RNG+ ACA+HD Sbjct: 182 AGFPQLTMVGLDVTRRVLLNEEHIRGLGDTPMGEYVRQSTSDYLHRYFQRNGIYACAMHD 241 Query: 245 PLAVGVALNCGLVTTRDYYVDVETRSELCDGQTVCDFQNRLNRPPNVTVCLEVDAPAFLE 304 PLAV VAL+ LV T+ YVD+ETRSELCDGQTVCDFQNRL + PN+ VCL+VDA AF Sbjct: 242 PLAVAVALDPKLVKTQKLYVDIETRSELCDGQTVCDFQNRLKKEPNINVCLDVDADAFFA 301 Query: 305 CFINSLN 311 F+ +L Sbjct: 302 RFLGALK 308 >A8FJ31.1 A8FJ31_BACP2 Purine nucleosidase EC=3.2.2.1 Length = 311 Score = 380 bits (977), Expect = e-104, Method: Compositional matrix adjust. Identities = 182/311 (58%), Positives = 233/311 (74%), Gaps = 1/311 (0%) Query: 1 MTGRKKVILDVDTGVDDALAIMLAVTSGRFDILGITTVSGNVSLDQATLNTCKILELLGV 60 M +KK+ILDVDTG+DDA+ I+LAV S +FD+LG+TTV GNVS+D ATLNTCK+LEL+ Sbjct: 1 MANKKKLILDVDTGIDDAIGILLAVKSQQFDMLGMTTVCGNVSVDAATLNTCKVLELVE- 59 Query: 61 SGQIPVYRGADKPLVREAVFEHRVHGSDGIGGALGDMPVTKQPEAEAAADFIIRQVLAQP 120 + IPV +G+ PL+R +EHRVHG DGIGGAL D+ K +A A DFII QV+ Sbjct: 60 ADDIPVIKGSATPLLRAPHYEHRVHGEDGIGGALKDVEPKKTADAGFAPDFIIDQVMQYS 119 Query: 121 GEVTLIMTAPLTNLANALQKCPELVQHAAEVIVMGGVVQGYGNITPTAEYNMYVDPEAAK 180 +VTL++T PLTNLA A++KCPEL+ H EVI MGGVVQG GN+TP AE+N Y DPEAAK Sbjct: 120 KQVTLVLTGPLTNLALAVKKCPELIHHVKEVIFMGGVVQGQGNVTPVAEFNTYADPEAAK 179 Query: 181 QVLAAGFPRLTLVGLDVTRRALLGAEDIQKLHNPVIREYVETSTAGYRARYYERNGVQAC 240 VL AGFP LT VGLDVTR+ LL E I + N + Y+ ST+ YR RY+ERNG+ AC Sbjct: 180 LVLDAGFPTLTQVGLDVTRKVLLTDERIDAIQNETLAHYIRESTSIYRQRYFERNGLWAC 239 Query: 241 ALHDPLAVGVALNCGLVTTRDYYVDVETRSELCDGQTVCDFQNRLNRPPNVTVCLEVDAP 300 A+HDPLAV +A++ LV+T+ ++VDVET+SE CDGQ +CDFQ++ + NV VC +VDA Sbjct: 240 AMHDPLAVSLAIDKQLVSTQAFHVDVETKSEFCDGQMICDFQHQWKKERNVQVCTDVDAD 299 Query: 301 AFLECFINSLN 311 AF + IN++N Sbjct: 300 AFFDLLINTMN 310 >B4AJZ7.1 B4AJZ7_BACPU Purine nucleosidase Length = 299 Score = 362 bits (928), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 178/311 (57%), Positives = 228/311 (73%), Gaps = 13/311 (4%) Query: 1 MTGRKKVILDVDTGVDDALAIMLAVTSGRFDILGITTVSGNVSLDQATLNTCKILELLGV 60 M +KK+ILDVDTG+DDA+ I+LAV S RFD+LGITTV GNVS+D ATLNTCK+LEL+ Sbjct: 1 MANKKKLILDVDTGIDDAIGILLAVKSNRFDMLGITTVCGNVSVDAATLNTCKVLELVE- 59 Query: 61 SGQIPVYRGADKPLVREAVFEHRVHGSDGIGGALGDMPVTKQPEAEAAADFIIRQVLAQP 120 + IPV +G+ PL+R +EHRVHG DGIGGAL D QP+ I QV+ Sbjct: 60 ADNIPVIKGSATPLLRAPHYEHRVHGEDGIGGALKD----AQPK--------IDQVIQYS 107 Query: 121 GEVTLIMTAPLTNLANALQKCPELVQHAAEVIVMGGVVQGYGNITPTAEYNMYVDPEAAK 180 +VTL++T PLTNLA A++KCPEL+ H EVI MGGVV+G GN+TP AE+N Y DPEAAK Sbjct: 108 KQVTLVLTGPLTNLALAVKKCPELILHVNEVIFMGGVVEGQGNVTPVAEFNTYADPEAAK 167 Query: 181 QVLAAGFPRLTLVGLDVTRRALLGAEDIQKLHNPVIREYVETSTAGYRARYYERNGVQAC 240 VL AGFP LT VGLDVTR+ LL E I + N + Y+ ST+ Y RY+ERNGV AC Sbjct: 168 LVLDAGFPSLTQVGLDVTRQVLLTDERIDAIQNETLAHYIRESTSIYCQRYFERNGVWAC 227 Query: 241 ALHDPLAVGVALNCGLVTTRDYYVDVETRSELCDGQTVCDFQNRLNRPPNVTVCLEVDAP 300 A+HDPLAV +A++ LV+T+ ++VDVET+SE CDGQ +CDFQ++ + NV VC++V+A Sbjct: 228 AMHDPLAVSLAIDKQLVSTQAFHVDVETKSEFCDGQMICDFQHQWEKERNVQVCMDVNAD 287 Query: 301 AFLECFINSLN 311 AF + IN++N Sbjct: 288 AFFDLLINTMN 298 >Q5WD21.1 Q5WD21_BACSK Inosine-uridine preferring nucleoside hydrolase Length = 310 Score = 336 bits (861), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 172/308 (55%), Positives = 216/308 (70%) Query: 5 KKVILDVDTGVDDALAIMLAVTSGRFDILGITTVSGNVSLDQATLNTCKILELLGVSGQI 64 +K++LDVDTGVDDALAI+LA S +L ITTV+GNVSL++AT+NT K+L LL G I Sbjct: 3 RKILLDVDTGVDDALAIILASKSTDAQLLAITTVNGNVSLEKATVNTLKVLTLLHKEGDI 62 Query: 65 PVYRGADKPLVREAVFEHRVHGSDGIGGALGDMPVTKQPEAEAAADFIIRQVLAQPGEVT 124 PV +GA PL R FEH VHG+DG+GGAL T QP A D+II Q PGE+T Sbjct: 63 PVIQGAHAPLRRPCFFEHSVHGNDGLGGALPHFEPTAQPADGYAPDYIIEQANRYPGELT 122 Query: 125 LIMTAPLTNLANALQKCPELVQHAAEVIVMGGVVQGYGNITPTAEYNMYVDPEAAKQVLA 184 L+MT PLTNLA AL+KCP+L + A V+ MGG +GN+TP AEYNMYVDPEAA++V+ Sbjct: 123 LVMTGPLTNLALALEKCPDLPKLVAGVVYMGGAAFTHGNVTPVAEYNMYVDPEAARKVIH 182 Query: 185 AGFPRLTLVGLDVTRRALLGAEDIQKLHNPVIREYVETSTAGYRARYYERNGVQACALHD 244 AGF L VGLDVTR+ALL E+I L + I+++++T TA Y RY RN V ACALHD Sbjct: 183 AGFAPLVQVGLDVTRKALLTRENIGSLVDSPIKQFIKTCTADYIKRYEARNTVAACALHD 242 Query: 245 PLAVGVALNCGLVTTRDYYVDVETRSELCDGQTVCDFQNRLNRPPNVTVCLEVDAPAFLE 304 PLAV AL L+ + +V+VET S+ CDGQTVCD Q RL +P NV V E+D+ AFL Sbjct: 243 PLAVAYALKPELLHLEEMFVEVETNSQFCDGQTVCDRQQRLGKPANVNVATEIDSDAFLS 302 Query: 305 CFINSLNQ 312 F++S+ Sbjct: 303 YFLHSIQN 310 >C0Z920.1 C0Z920_BREBN Putative nucleosidase Length = 310 Score = 226 bits (576), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 133/310 (42%), Positives = 186/310 (60%), Gaps = 8/310 (2%) Query: 5 KKVILDVDTGVDDALAIMLAVTSGRFDILGITTVSGNVSLDQATLNTCKILELLGVSGQI 64 +K+ILDVDTG+DDA+AI AV S ++ GITT GN+++++AT NT ++LELLG S +I Sbjct: 2 EKIILDVDTGIDDAMAIAYAVHSPALEVCGITTTFGNITVEEATRNTLQVLELLGAS-EI 60 Query: 65 PVYRGADKPLVREAVFEHRV-HGSDGIGGALGDMPVTK-QPEAEAAADFIIRQVLAQPGE 122 PVY+GA KP+VRE + R+ HG +G+G + P TK QP++ AA F+I + P E Sbjct: 61 PVYQGASKPIVRELTGKARLFHGENGLGNVVLPDPSTKAQPQS--AAQFLISTIKENPHE 118 Query: 123 VTLIMTAPLTNLANALQKCPELVQHAAEVIVMGGVVQGYGNITPTAEYNMYVDPEAAKQV 182 VTL+ + NLA A+ PE+V V+VMGG V GN TP AE N+ DPEAA + Sbjct: 119 VTLVTVGSMANLARAIMAAPEIVSLVKRVVVMGGAVTVPGNRTPVAEANICADPEAAAYI 178 Query: 183 LAAGFPRLTLVGLDVTRRALLGAEDIQKLHNPVIR--EYVETSTAGYRARYYERNGVQAC 240 +G P +TLVGLDVT + LL E +Q+ R + Y Y ++ C Sbjct: 179 FQSGIP-VTLVGLDVTMQTLLTREHLQEWRAKDTRLSHFFADMCEVYMDAYATVGNLRGC 237 Query: 241 ALHDPLAVGVALNCGLVTTRDYYVDVETRSELCDGQTVCDFQNRLNRPPNVTVCLEVDAP 300 LHDPLAVGV ++ V + +V V+T D +T+ D + +PPNV VCLEVD Sbjct: 238 GLHDPLAVGVVIDPTFVKSVPMHVAVDTSGGASDARTIGDRRPHPAQPPNVDVCLEVDHE 297 Query: 301 AFLECFINSL 310 F+ F+ ++ Sbjct: 298 RFVSHFLQNV 307 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,908,908,968 Number of extensions: 78128489 Number of successful extensions: 198228 Number of sequences better than 1.0e-05: 1694 Number of HSP's gapped: 193294 Number of HSP's successfully gapped: 1712 Length of query: 320 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 185 Effective length of database: 1,788,859,178 Effective search space: 330938947930 Effective search space used: 330938947930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)