BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0007.p (493 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4L4.1 B3K4L4_9BACI Drug resistance transporter, EmrB/QacA sub... 428 e-118 B1D531.1 B1D531_9BACL Drug resistance transporter, EmrB/QacA sub... 374 e-101 B1DCQ7.1 B1DCQ7_9BACL Drug resistance transporter, EmrB/QacA sub... 354 9e-96 Q1DBQ7.1 Q1DBQ7_MYXXD Drug resistance transporter, EmrB/QacA sub... 350 2e-94 Q97TH3.1 Q97TH3_CLOAB Permease, MDR related 311 9e-83 >B3K4L4.1 B3K4L4_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 472 Score = 428 bits (1100), Expect = e-118, Method: Compositional matrix adjust. Identities = 220/473 (46%), Positives = 305/473 (64%), Gaps = 9/473 (1%) Query: 4 HTARNSSFWLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATGV 63 +T SSFWLI+ AVFFGNF+A+LS TTINVAFPV M+ FHA + T QW++ GYLLATG+ Sbjct: 2 NTDEKSSFWLIMSAVFFGNFLAVLSITTINVAFPVVMEQFHATLSTTQWLMAGYLLATGI 61 Query: 64 IAPVVGYFGDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTTM 123 +AP+VGY GD+ SYK L+V AL+GFTL S LC++AW+I +L+ FRI QGVFGG+IIP TM Sbjct: 62 VAPIVGYMGDQLSYKRLFVLALTGFTLSSLLCVVAWNIETLIAFRITQGVFGGMIIPITM 121 Query: 124 TMIYQFIEKEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIAV 183 T+IYQ +E+Q GPTLGGWL YFGW+S+F++N+PIG+I+I + Sbjct: 122 TIIYQVFPRERQAYAMGLWSLASMLAPVIGPTLGGWLVQYFGWKSIFILNVPIGLISIVI 181 Query: 184 ALKCLPFQRNAGETKSFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXXXXX 243 K +PF R G+ K+FDL GF+TV+ S+ ++L+F+ GN WGW SWK Sbjct: 182 VSKFIPFYRLTGK-KTFDLLGFLTVVAGSSCLLLSFSHGNDWGWGSWKTISLLVAGILLL 240 Query: 244 MYFIRRELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPLT 303 ++FI+ EL + PLL LKVF+ RF YSLI+NCI+T++LY+GT L+P++LQ + + +P+ Sbjct: 241 LFFIKWELRISSPLLQLKVFKFPRFRYSLILNCIVTISLYTGTLLVPLYLQTVLKLSPMD 300 Query: 304 TALVLLPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQLAW 363 T L++LPG+I MA SP++GK Y ++GPF +T S + T+ + ++ Sbjct: 301 TGLIMLPGAIVMAAASPIIGKFYDRIGPFRLVITGVLIIVAATLAFSGIGTNTSVLYISM 360 Query: 364 LMAVRNIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATGXXXXXXXXXXXXXXXX 423 L VR +GIAL MP+TNA MS+V + +GHASS+TNW RQ Sbjct: 361 LQLVRCLGIALCNMPLTNAAMSAVSSEYSGHASSITNWARQGLASLSIGIFSALVVTRTS 420 Query: 424 THQKELXXXXXXXXXLIKAQGMTMGVQDVFLTATVIGILAIPLTFLLRTGTAK 476 + + TMG+ DVF+ T++ ++AIPLTFLLR K Sbjct: 421 YYMGA--------GTVTPRAATTMGIDDVFMIGTMVAVVAIPLTFLLRVKKKK 465 >B1D531.1 B1D531_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 483 Score = 374 bits (961), Expect = e-101, Method: Compositional matrix adjust. Identities = 199/480 (41%), Positives = 287/480 (59%), Gaps = 1/480 (0%) Query: 1 LNNHTARNSSFWLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLA 60 +N +++N FW I++A+FFG F++IL +TINVA +FM+DFH+++ TVQW +TG++L+ Sbjct: 1 MNTSSSQNVRFWPIMIAIFFGAFLSILGISTINVALTIFMQDFHSDLSTVQWTLTGFMLS 60 Query: 61 TGVIAPVVGYFGDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIP 120 G IAP+ GY G+K+ YK +Y++A+ GF +FS LC +W+ SL+ FRI+QG+F GL++P Sbjct: 61 LGTIAPLSGYLGEKFGYKNVYLYAMIGFVIFSLLCGFSWNAGSLIAFRIIQGIFTGLVMP 120 Query: 121 TTMTMIYQFIEKEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIA 180 TMT+I+Q I KEKQ GPTL GWL F W +F +N+PIGI+A Sbjct: 121 ATMTIIFQIIPKEKQPFAVSIWGLSGMLAPAFGPTLSGWLIQQFDWRWIFFMNVPIGIVA 180 Query: 181 IAVALKCLPFQRNAGETKSFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXX 240 I + +P R G K D G TV+LSS ++LA ++G WGW S K Sbjct: 181 ILFIIAMIPDVR-IGTAKVLDKLGLTTVVLSSVALLLALSEGRQWGWDSAKVLGLFGLGA 239 Query: 241 XXXMYFIRRELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQST 300 + FI EL + PLLNL+VF RF+ +L+ N IIT++LYSGT L+P+FLQ++QQ T Sbjct: 240 VMLVLFIWHELRTQNPLLNLRVFGNYRFSITLVANTIITISLYSGTLLVPLFLQNVQQIT 299 Query: 301 PLTTALVLLPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQ 360 P+ T L+LLP S+ MA+ P+ GKLY +VG W ++E++HL ++H Sbjct: 300 PMDTGLILLPSSLVMALAMPIAGKLYPRVGAKWMTIGGLVLIIFGSYEMTHLHTDSSHFY 359 Query: 361 LAWLMAVRNIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATGXXXXXXXXXXXXX 420 + + MAVRN+G++ A + AGM +P Q+ GHASSVTNWVR G Sbjct: 360 VIFWMAVRNLGVSFAAAATSTAGMEEIPPQMVGHASSVTNWVRNVGGSFAIALFTSLLAS 419 Query: 421 XXXTHQKELXXXXXXXXXLIKAQGMTMGVQDVFLTATVIGILAIPLTFLLRTGTAKPQAA 480 TH +L +I T+ V DVF AT I ++AIP T ++R + + A Sbjct: 420 HTITHVSDLTQAGTENAKIIPLFAFTLSVNDVFTIATYIAVIAIPFTLIIRKQAKRSKNA 479 >B1DCQ7.1 B1DCQ7_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 484 Score = 354 bits (909), Expect = 9e-96, Method: Compositional matrix adjust. Identities = 184/481 (38%), Positives = 280/481 (58%), Gaps = 6/481 (1%) Query: 3 NHTARNSSFWLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATG 62 N ++ FW +ILA+FFGNF +IL++ T+ VA P MKD H+++ QW+ TG++LA G Sbjct: 2 NAKQDDNRFWFVILAIFFGNFSSILNSGTVTVALPFMMKDLHSDLNDTQWVTTGFMLAMG 61 Query: 63 VIAPVVGYFGDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTT 122 IAP+VGY GDK SYK LY+F+L GF + S LC +W+I SLV+FRILQG+F G I+P T Sbjct: 62 SIAPIVGYLGDKISYKRLYMFSLVGFLICSVLCGCSWNIGSLVLFRILQGLFCGAILPAT 121 Query: 123 MTMIYQFIEKEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIA 182 +T++YQ + EKQ GPTLGG LT Y+GW+++F +N+P+ ++AI Sbjct: 122 LTIVYQAVPAEKQALGVSLWSVASMFAPAIGPTLGGLLTEYYGWKAIFFMNIPLALLAIV 181 Query: 183 VALKCLPFQR-NAGETKSFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXXX 241 +A + LP Q+ N G S DL G +T + S +++ F +GN GW S Sbjct: 182 LASRYLPMQQVNKGS--SLDLAGLLTAVTGSTSLMMYFTQGNKIGWFSGWGILWITLGVV 239 Query: 242 XXMYFIRRELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTP 301 FIRREL+ K PLLNL+VFR FT+ +++NCI+T+ LY+G FL+P+++++ S+ Sbjct: 240 SIALFIRRELTAKAPLLNLRVFRHTTFTFGIVVNCILTIGLYAGAFLLPIYMENALGSSS 299 Query: 302 LTTALVLLPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQL 361 LT L++LPG++ M S + G+L+ K+ P + +TW S L+L T++ + Sbjct: 300 LTVGLMMLPGALLMIGASLLSGRLHGKIRPEYPLLGGAFLLIIATWAFSRLSLTTSYAFI 359 Query: 362 AWLMAVRNIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATGXXXXXXXXXXXXXX 421 M R +G+ + + MS++PK+ TGHAS++TNW+RQ Sbjct: 360 IGWMICRYVGLGFSSPNIMTLSMSAIPKESTGHASAITNWLRQVIASFAIGIFSSVYSVR 419 Query: 422 XXTHQKELXXXXXXXXXLIKAQGMTMGVQDVFLTATVIGILAIPLTFLL--RTGTAKPQA 479 TH EL L + + + D F+ A V+ ++AIP +L R+ + +A Sbjct: 420 TQTHFTELSSSGGVKTSL-QETASVLAMNDTFIVACVVTVVAIPFILVLSSRSRISGEKA 478 Query: 480 A 480 A Sbjct: 479 A 479 >Q1DBQ7.1 Q1DBQ7_MYXXD Drug resistance transporter, EmrB/QacA subfamily Length = 484 Score = 350 bits (899), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 180/471 (38%), Positives = 277/471 (58%), Gaps = 4/471 (0%) Query: 4 HTARNSS---FWLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLA 60 H+A + FW + +F G+FM++LS++TI +A P + F AE+ +VQW +TG++LA Sbjct: 2 HSAAEPTVPRFWPFMFTLFVGSFMSVLSSSTITIAIPELQRHFGAELSSVQWTLTGFMLA 61 Query: 61 TGVIAPVVGYFGDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIP 120 G AP+ GY G ++S+K++YV +L F + S LC +AW SLV FR +QG F G I+P Sbjct: 62 MGTSAPLTGYLGGRFSFKWVYVASLVAFLVASVLCGVAWDTRSLVAFRFVQGAFCGAIMP 121 Query: 121 TTMTMIYQFIEKEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIA 180 TMT+IYQ + +E+Q GPTL GWL W LF +N+P+G+ A Sbjct: 122 VTMTLIYQLLPRERQALAASLWTLSSMLAPAFGPTLAGWLMTLGNWRWLFFMNVPLGLAA 181 Query: 181 IAVALKCLPFQRNAGETKSFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXX 240 +A+A++ +P+ R K+FD PG +TVI + +++A ++G +WGWT K Sbjct: 182 VAMAVRAVPYYRLE-VPKAFDFPGLLTVITGTLALLIALSQGRAWGWTDSKTVSLAVLGT 240 Query: 241 XXXMYFIRRELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQST 300 +YF+ R+L PLL+L+V R+ +L+I+ IIT++LYSG FLIP+FLQ+IQ T Sbjct: 241 VSLVYFVVRQLRTHAPLLDLRVLANGRYVVTLLISSIITISLYSGAFLIPLFLQEIQGVT 300 Query: 301 PLTTALVLLPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQ 360 PL T L+LLP S++MA++ P+VG++Y K+GP T+ LS LT +H Sbjct: 301 PLKTGLILLPASLSMALLMPLVGRMYGKLGPSTLMTAGVLLIAGGTYALSRLTPDVSHTY 360 Query: 361 LAWLMAVRNIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATGXXXXXXXXXXXXX 420 + M VRN+GI+L+ MP +NAGM + ++++GHASS++NW+R G Sbjct: 361 VLMWMLVRNVGISLSIMPASNAGMEQLSRELSGHASSISNWLRNVFGAFSIAIFTSLLSS 420 Query: 421 XXXTHQKELXXXXXXXXXLIKAQGMTMGVQDVFLTATVIGILAIPLTFLLR 471 + L I+ G+ V+L ATVI ++A+PL+ ++R Sbjct: 421 FAAREAQVLAREGMTERRDIELLSFVTGINQVYLVATVIALVAVPLSLMVR 471 >Q97TH3.1 Q97TH3_CLOAB Permease, MDR related Length = 481 Score = 311 bits (797), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 170/483 (35%), Positives = 278/483 (57%), Gaps = 4/483 (0%) Query: 4 HTARNSSFWLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATGV 63 +S+W +++A+F G+F+ L T+TIN+A P MK F A + TV+W +TG++LA G Sbjct: 2 EETNKTSYWPMMIAIFLGSFLVSLGTSTINLALPFLMKSFSANLDTVKWTLTGFMLAMGT 61 Query: 64 IAPVVGYFGDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTTM 123 +AP+ Y G+++SYK +Y+ ++ GF L S LC+ + +I +L+I RI+QG F G+I+P TM Sbjct: 62 MAPLTAYLGERFSYKRVYLTSIVGFILASILCIFSINITTLIISRIIQGAFSGVIVPATM 121 Query: 124 TMIYQFIEKEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIAV 183 +IYQ + K++Q GPTL G+L YF W+S+F+IN+PIG+I + + Sbjct: 122 AIIYQAVPKKRQVSATSLWSMASMLAPAIGPTLSGFLIQYFSWQSIFIINIPIGLIILIL 181 Query: 184 ALKCLP-FQRNAGETKSFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXXXX 242 LK +P F+ N E SFD GF ++L S +++AF++G GWTS Sbjct: 182 GLKYIPYFKLNVPE--SFDFKGFGFIVLGSLALLIAFSEGGVLGWTSLPIIFSLAIGVIL 239 Query: 243 XMYFIRRELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPL 302 F+ R LS+ +L+L VF+ ++++S+++ I+ ++LY+GT L P+FLQ++Q + L Sbjct: 240 LGIFVYRSLSMDSSILDLCVFKYKKYSFSVVVYSIVMMSLYAGTLLTPIFLQNLQHMSAL 299 Query: 303 TTALVLLPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQLA 362 + ++LLP S+ MA+ P+VGKLY+K+ ++EL+HLT+ T+ + Sbjct: 300 DSGIILLPASLCMALAMPIVGKLYNKINSRIIIMTGILLIALGSFELTHLTVTTSKAFII 359 Query: 363 WLMAVRNIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATGXXXXXXXXXXXXXXX 422 M VRN+GI+ MPVT +GM+ +PK I+GH SSV NW+ + Sbjct: 360 VWMIVRNVGISFCTMPVTYSGMTVLPKAISGHGSSVNNWLARLVSSLSMAIFASLLTSRA 419 Query: 423 XTHQKELXXXXXXXXXLIKAQGMTMGVQDVFLTATVIGILAIPLTFLLRTGTAKPQAAVL 482 H L + ++ G MG+ DV+ + +I ++A+P ++ L K + Sbjct: 420 SMHVTSL-IKSGKAHIVAQSTGYVMGINDVYFVSFIIILIALPFSYFLNNRKVKTRETSK 478 Query: 483 NVA 485 VA Sbjct: 479 EVA 481 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,454,927,425 Number of extensions: 88114048 Number of successful extensions: 393714 Number of sequences better than 1.0e-05: 8144 Number of HSP's gapped: 378891 Number of HSP's successfully gapped: 8169 Length of query: 493 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 353 Effective length of database: 1,741,754,103 Effective search space: 614839198359 Effective search space used: 614839198359 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)