BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0001.p (309 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAY9.1 B3KAY9_9BACI Auxin Efflux Carrier 271 5e-71 C0ZKJ3.1 C0ZKJ3_BREBN Conserved hypothetical membrane protein 117 2e-24 Q9K9H7.1 Q9K9H7_BACHD BH2670 protein 102 6e-20 Q5WF91.1 Q5WF91_BACSK Transporter 98 1e-18 C0UZJ0.1 C0UZJ0_9BACT Predicted permease 97 1e-18 >B3KAY9.1 B3KAY9_9BACI Auxin Efflux Carrier Length = 307 Score = 271 bits (694), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 145/304 (47%), Positives = 197/304 (64%), Gaps = 1/304 (0%) Query: 1 LIDKVLSTLVEVIVPLSIPVISGALLGRYKNLDTKPLLTLYLYFLSPAIILDTLATAEIS 60 +I L+T+ V +P+S+PVI GALL R+K ++TK L L LY LSPA+I +TL A I+ Sbjct: 1 MISSFLATIYSVFLPISLPVIGGALLKRFKGIETKGLSALSLYVLSPALIFETLEKASIT 60 Query: 61 FGDVYKTLAFSXXXXXXXXXXXXXXGRVLKLAPAEAAGLTLISTFTNSVNYGLPLVLLAF 120 +V T+AF G++L L+ E +GL L + FTNSVNYGLPLVLLAF Sbjct: 61 SNEVTVTVAFCLLNVIILWALSAFAGKMLGLSQTEKSGLALTTIFTNSVNYGLPLVLLAF 120 Query: 121 GQLGLDKASVYVIAQMVIVNTIGVYFAARSQFSAGSAVRSVFSXXXXXXXXXXXXXXXXX 180 GQ GLDKASVYVI Q++I+NT+GVY AARS FSA A+++VF+ Sbjct: 121 GQAGLDKASVYVIVQIIIMNTLGVYLAARSHFSAVKAIKTVFTLPSVYAAAFAVLFRLTD 180 Query: 181 XHMPQEIASGVSMVAGAYSPVVLAILGVQMVRVQTAQVARNV-QLAFWTGMSVRXXXXXX 239 +P+ + +GVSM++ Y+P+ L ILG QMV V+ + V + FW+GM++R Sbjct: 181 FQLPEGLNTGVSMLSATYAPLALVILGAQMVGVREGGASLAVSKRGFWSGMAMRMLVGPL 240 Query: 240 XXXXXXXXXNISGTLFAVLLILASMPVAVNSVVLAERFGSSPALVSRCILWTTLASFLVL 299 +I GTLFAV+LIL++MP AVN+V+LAE + ++P LVSRCILWTTLASF+VL Sbjct: 241 VAWGLLAMLSIEGTLFAVILILSAMPAAVNAVILAEEYDAAPKLVSRCILWTTLASFIVL 300 Query: 300 PILI 303 P +I Sbjct: 301 PAMI 304 >C0ZKJ3.1 C0ZKJ3_BREBN Conserved hypothetical membrane protein Length = 297 Score = 117 bits (293), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 84/302 (27%), Positives = 139/302 (46%), Gaps = 12/302 (3%) Query: 9 LVEVIVPLSIPVISGALLGRYKNLDTKPLLTLYLYFLSPAIILDTLATAEISFGDVYKTL 68 VEVI+P+ + +SG +L R +D KP+ +L +Y LS A++ T ++ ++ + Sbjct: 3 FVEVILPVLLIFLSGYILQRIFKMDLKPISSLAIYILSTALVFRTFYKTQLDL-QLFYIV 61 Query: 69 AFSXXXXXXXXXXXXXXGRVLKLAPAEAAGLTLISTFTNSVNYGLPLVLLAFGQLGLDKA 128 S R+ K + + L L + F NS NYG P++L AFG+ G A Sbjct: 62 VISLLLLAALVVITQLTARMFKYDKQQESALMLATAFMNSGNYGTPIILFAFGEAGFTYA 121 Query: 129 SVYVIAQMVIVNTIGVYFAARSQFSAGSAVRSVFSXXXXXXXXXXXXXXXXXXHMPQEIA 188 ++ +++ GVYFA+R G+A++++F +PQ Sbjct: 122 VQIMVFHSIMMGVFGVYFASRGGNGMGTAIKAIFKQPSNYAVVLAILLQQLQIVIPQSYY 181 Query: 189 SGVSMVAGAYSPVVLAILGVQMVRVQTAQVARNVQLAFWTGMS----VRXXXXXXXXXXX 244 + +VA A PVV+ ILG+Q+ V T + W G+S +R Sbjct: 182 QAIDLVAQAAIPVVMLILGMQLANVSTKALE-------WQGLSAASVIRLVASPLLAYLI 234 Query: 245 XXXXNISGTLFAVLLILASMPVAVNSVVLAERFGSSPALVSRCILWTTLASFLVLPILIS 304 I L VL++LA+MP A + + A +F P VS +L TT+ S L +L++ Sbjct: 235 CLLFPIDPLLQKVLVVLAAMPSAATTAIYAIQFNMRPQFVSSSVLVTTVISIGTLTLLLN 294 Query: 305 IV 306 I+ Sbjct: 295 IL 296 >Q9K9H7.1 Q9K9H7_BACHD BH2670 protein Length = 298 Score = 102 bits (253), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 80/307 (26%), Positives = 142/307 (46%), Gaps = 14/307 (4%) Query: 6 LSTLVEVIVPLSIPVISGALLGRYKNLDTKPLLTLYLYFLSPAIILDTLATAEISFGDVY 65 ++ +V++P+ + G ++ +++++D KP+ T+ +Y L+P+++ T AE++ +Y Sbjct: 1 MTIFFQVVLPVILVFFIGFIVQKWRDVDIKPISTVAVYILTPSLVFRTFYEAELNLQYLY 60 Query: 66 KTLAFSXXXXXXXXXXXXXXGRVLK-LAPAEAAGLTLISTFTNSVNYGLPLVLLAFGQLG 124 L VL+ + A +GL L + F NS NYG P++L A+G G Sbjct: 61 MFLFAMLLLFALIAINKVYA--VLRGYSQATESGLVLSTAFMNSGNYGAPIILFAYGNTG 118 Query: 125 LDKASVYVIAQMVIVNTIGVYFAARSQFSAGSAVRSVFSXXXXXXXXXXXXXXXXXXHMP 184 A +++ Q VI+N GVY+AAR + A+R+V +P Sbjct: 119 FAFAVSFMVLQSVIMNFFGVYYAARGKAGVRMAIRAVMQMPATYAVIVALALQAIPVEIP 178 Query: 185 QEIASGVSMVAGAYSPVVLAILGVQMVRVQTAQVARNVQLAFWT----GMSVRXXXXXXX 240 V ++A A P V+ ILG+Q+ ++ W+ G +R Sbjct: 179 ANFLMTVDIIAEAAVPTVMIILGMQLAMIKWEHFD-------WSKITYGTILRLIVSPLI 231 Query: 241 XXXXXXXXNISGTLFAVLLILASMPVAVNSVVLAERFGSSPALVSRCILWTTLASFLVLP 300 L VL++ A+MP A V+ A +F + P LV+ L TTL S + + Sbjct: 232 AFGITLVFPFDPLLAKVLIVSAAMPSAATIVMYAVQFQAEPRLVTSITLITTLFSVVTIT 291 Query: 301 ILISIVG 307 +L++I+G Sbjct: 292 VLLAILG 298 >Q5WF91.1 Q5WF91_BACSK Transporter Length = 298 Score = 97.8 bits (242), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 82/305 (26%), Positives = 137/305 (44%), Gaps = 12/305 (3%) Query: 6 LSTLVEVIVPLSIPVISGALLGRYKNLDTKPLLTLYLYFLSPAIILDTLATAEISFGDVY 65 + ++V++P+ +G LL R LD + + ++ +Y ++P ++ T T + Y Sbjct: 1 MGIFLQVVLPVCSIFAAGFLLQRAFMLDIRSVSSVAIYIMTPCLVFRTFYTGNLDAQYAY 60 Query: 66 KTLAFSXXXXXXXXXXXXXXGRVLKLAPAEAAGLTLISTFTNSVNYGLPLVLLAFGQLGL 125 L + ++ KL+ +E +G+ L ++F N NYG P++L A+G+ Sbjct: 61 MALF-ALLLLASLIVITKGYAKLRKLSLSEESGMILATSFMNVGNYGTPIILFAYGEGAF 119 Query: 126 DKASVYVIAQMVIVNTIGVYFAARSQFSAGSAVRSVFSXXXXXXXXXXXXXXXXXXHMPQ 185 D A +++ Q +I+N GVY+AAR + + AV +V +P Sbjct: 120 DLAVSFMVLQSIIMNIFGVYYAARGKAAMKGAVLAVLKMPATYATVLALGLNVGGVALPP 179 Query: 186 EIASGVSMVAGAYSPVVLAILGVQMVRVQTAQVARNVQLAFWTGMSV----RXXXXXXXX 241 + + +VA A P V+ ILG+Q+ + A W ++V R Sbjct: 180 NLFQPIDIVAEAAIPTVMLILGMQLAMIPLRGFA-------WEKLTVATVIRMAISPVVA 232 Query: 242 XXXXXXXNISGTLFAVLLILASMPVAVNSVVLAERFGSSPALVSRCILWTTLASFLVLPI 301 IS L VL+I A+MP A VV A +F S P LVS L TTL S L Sbjct: 233 FVIVSFLPISEILSKVLIISAAMPSAATIVVYAVQFDSEPKLVSSITLVTTLVSLFSLTF 292 Query: 302 LISIV 306 ++I+ Sbjct: 293 FLAIL 297 >C0UZJ0.1 C0UZJ0_9BACT Predicted permease Length = 302 Score = 97.4 bits (241), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 73/291 (25%), Positives = 130/291 (44%), Gaps = 4/291 (1%) Query: 5 VLSTLVEVIVPLSIPVISGALLGRYKNLDTKPLLTLYLYFLSPAIILDTLATAEISFGDV 64 +LS + VP+ I V++G + R + + L L LY LSPA++ +L ++ ++ Sbjct: 1 MLSIFINTTVPVFIIVLAGMVAARCGVMHAQSLARLSLYVLSPALVFSSLVNTSVTLSNI 60 Query: 65 YKTLAFSXXXXXXXXXXXXXXGRVLKLAPAEAAGLTLISTFTNSVNYGLPLVLLAFGQLG 124 + +L + P + L + NS N+GLP++L AFG G Sbjct: 61 LRIGLSLLILAIFTYLLGTLVCELLGVEPYTKSSYLLSTILMNSGNFGLPVILFAFGDEG 120 Query: 125 LDKASVYVIAQMVIVNTIGVYFAARSQFSAGSAVRSVFSXXXXXXXXXXXXXXXXXXHMP 184 L AS+Y + Q ++ NT+GVY A+R SA+ + F +P Sbjct: 121 LAVASIYFVIQSLLTNTLGVYIASRGMADPKSALMNTFKLPTLYAIVVALPFLIFHFPLP 180 Query: 185 QEIASGVSMVAGAYSPVVLAILGVQMVRVQTAQVARNVQLAFWTGMSVRXXXXXXXXXXX 244 Q + + ++ A P++L +LG Q+ R++ + + F + R Sbjct: 181 QVVNRPIDLLGSAAIPILLLMLGAQL-RLRLKAI---ISPLFVAALITRLVISPLIAYMI 236 Query: 245 XXXXNISGTLFAVLLILASMPVAVNSVVLAERFGSSPALVSRCILWTTLAS 295 + G + V ++ ++MP AVN+++LA F S +S I ++TL S Sbjct: 237 GRVMGLEGLILQVFVVESAMPTAVNTIMLATVFKSDTDFLSNVIAFSTLVS 287 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.137 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,268,453,423 Number of extensions: 39437527 Number of successful extensions: 169989 Number of sequences better than 1.0e-05: 217 Number of HSP's gapped: 169903 Number of HSP's successfully gapped: 217 Length of query: 309 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 174 Effective length of database: 1,788,859,178 Effective search space: 311261496972 Effective search space used: 311261496972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)