BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0011.p (306 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8X1.1 B3K8X1_9BACI Sporulation peptidase YabG 437 e-121 B1DII7.1 B1DII7_9BACL Sporulation peptidase YabG 343 1e-92 C1TIA1.1 C1TIA1_9FIRM Sporulation peptidase YabG 251 6e-65 Q65PH8.1 Q65PH8_BACLD YabG 248 6e-64 A5A6A5.1 A5A6A5_BACLD Spore coat assembly Peptidase U57, YabG 247 8e-64 >B3K8X1.1 B3K8X1_9BACI Sporulation peptidase YabG Length = 300 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 215/301 (71%), Positives = 255/301 (84%), Gaps = 3/301 (0%) Query: 5 MNLGDLVVRKSYGGDVTFRVENIVQNG-AVIKGTEFRLLADAPLDDLVQVPPTRITERGQ 63 MNLGDLVVRKSYGGDVTFRVE + QN VIKGTEFRLLADAP+DDLV VP ++ER + Sbjct: 1 MNLGDLVVRKSYGGDVTFRVEGMRQNNNIVIKGTEFRLLADAPVDDLVTVPHPTLSERTK 60 Query: 64 QAQIKATESLTKLRKDRQEQSQRSGESLTGVWPQSPKEAAYFEVPGKVLHLDGDALYLKK 123 QAQIKA ESL +L+K RQEQS+R SL + YF+VPGKVLHLDGD YLKK Sbjct: 61 QAQIKANESLDRLQKQRQEQSERQLASLRHTEGGQLADKGYFDVPGKVLHLDGDPQYLKK 120 Query: 124 SLSLYEQLRIPAEGHHVHESKMAETLYRLLPHVRPDIVVITGHDGVLKQQHTYDLYSLNS 183 S++LY QLR+PAEGH+++ES MA++LYRLLP VRPDIVVITGHDGVLK++ YDLYSL+S Sbjct: 121 SMTLYNQLRVPAEGHYINESGMADSLYRLLPRVRPDIVVITGHDGVLKRRQPYDLYSLDS 180 Query: 184 YKNSQNFVTAVKVAREYERNFDTLXXXXGGGPCQSHFEALLGAGANFASSPGRILIHALD 243 YKNS NFVTA++VAR+YERN D+L G CQSHFEALL AGANFASSPGRILIHALD Sbjct: 181 YKNSHNFVTAIQVARQYERNLDSLVIVAGA--CQSHFEALLQAGANFASSPGRILIHALD 238 Query: 244 PVYIAAKASFTSIRDSVSLGDVLNNTISGSQGMGGIETRGSFRIGMPRLQNLSTLKVAPS 303 PVY+AAKA++TS+RD+V++ DV+++TISGSQG+GGIETRGS+RIG+P+LQ+LSTLKV PS Sbjct: 239 PVYVAAKAAYTSVRDTVNIHDVVHHTISGSQGVGGIETRGSYRIGLPKLQDLSTLKVTPS 298 Query: 304 V 304 V Sbjct: 299 V 299 >B1DII7.1 B1DII7_9BACL Sporulation peptidase YabG Length = 297 Score = 343 bits (880), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 169/287 (58%), Positives = 215/287 (74%), Gaps = 5/287 (1%) Query: 5 MNLGDLVVRKSYGGDVTFRVENIVQNGAVIKGTEFRLLADAPLDDLVQVPPTRITERGQQ 64 M GDLVVR SYGGDV FRVE + + A++KGT++RLLADAP+ DL V T QQ Sbjct: 1 MKQGDLVVRNSYGGDVLFRVEEMRSDQALLKGTDYRLLADAPIPDLSVVRDPESTSARQQ 60 Query: 65 AQIKATESLTKLRKDRQEQSQRSGESLTGVWPQSPKEAAYFEVPGKVLHLDGDALYLKKS 124 +IK +SL ++R+++ + S + QS +YFE+PGKVLHLDGD YL+KS Sbjct: 61 VKIKVNDSLKRMRQEKAHLTVLSSPDRAALQQQS---QSYFELPGKVLHLDGDGNYLRKS 117 Query: 125 LSLYEQLRIPAEGHHVHESKMAETLYRLLPHVRPDIVVITGHDGVLKQQHTYDLYSLNSY 184 + LY+ +++PA G H HES+MA+ LYRLLP V+PDIVVITGHDGVLK + DLYSL+SY Sbjct: 118 MQLYQTMKVPAHGVHAHESQMAQLLYRLLPQVKPDIVVITGHDGVLKNREGEDLYSLSSY 177 Query: 185 KNSQNFVTAVKVAREYERNFDTLXXXXGGGPCQSHFEALLGAGANFASSPGRILIHALDP 244 KNSQ+FV AV+VAR+YE+N D L G CQSHFEALL +G+NFASSPGRILIHALDP Sbjct: 178 KNSQHFVNAVRVARQYEKNMDGLVVVAGA--CQSHFEALLQSGSNFASSPGRILIHALDP 235 Query: 245 VYIAAKASFTSIRDSVSLGDVLNNTISGSQGMGGIETRGSFRIGMPR 291 VYIA KAS+TS+R+S++L DV++ TISG G+GGIET G R+G+P+ Sbjct: 236 VYIAIKASYTSVRESINLSDVIHGTISGIDGVGGIETMGRHRMGLPK 282 >C1TIA1.1 C1TIA1_9FIRM Sporulation peptidase YabG Length = 293 Score = 251 bits (641), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 131/292 (44%), Positives = 185/292 (63%), Gaps = 15/292 (5%) Query: 5 MNLGDLVVRKSYGGDVTFRVENIVQN-----GAVIKGTEFRLLADAPLDDLVQVPPTRIT 59 + +GD+V R SYG D+ F+V NI N A +KG + RL A A + DLV++ PT + Sbjct: 3 IKVGDIVGRSSYGCDIFFKVINIYTNVAGQQMAKLKGMDLRLTATAAVSDLVKIDPTHVN 62 Query: 60 ERGQQAQIKATESLTKLRKDRQEQSQRSGESLTGVWPQSPKEAAYFEVPGKVLHLDGDAL 119 + + E + ++ RQE QRS TG +S F++PGKVLH+DGD Sbjct: 63 AYWHKMMSRNYEEMKRVFSRRQEAHQRSIMRNTGDRIES------FDIPGKVLHVDGDKD 116 Query: 120 YLKKSLSLYEQLRIPAEGHHVHESKMAETLYRLLPHVRPDIVVITGHDGVLKQQHTYDLY 179 YL ++ Y+Q+ IPA G++V E K E + LL PDIVV+TGHDG +K Q D Sbjct: 117 YLNLCMTTYKQMSIPAHGYNVSEEKQPEKIIELLEKHNPDIVVLTGHDGFIKGQK--DFS 174 Query: 180 SLNSYKNSQNFVTAVKVAREYERNFDTLXXXXGGGPCQSHFEALLGAGANFASSPGRILI 239 L +Y NS+++V A+K AR YE+N D L G CQSH+EA+L AGANFASSP R+LI Sbjct: 175 DLKNYHNSRHYVEAIKSARRYEKNMDDLIIFAGA--CQSHYEAMLDAGANFASSPKRVLI 232 Query: 240 HALDPVYIAAKASFTSIRDSVSLGDVLNNTISGSQGMGGIETRGSFRIGMPR 291 HA DPV+I K ++TSI +++ L +V++ TI+G +G+GG+ETRG R+G+P+ Sbjct: 233 HAFDPVFIVEKIAYTSIYEAIPLKEVISGTITGFEGVGGMETRGKHRLGIPK 284 >Q65PH8.1 Q65PH8_BACLD YabG Length = 299 Score = 248 bits (632), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 134/293 (45%), Positives = 185/293 (63%), Gaps = 12/293 (4%) Query: 3 FEMNLGDLVVRKSYGGDVTFRVENIVQ--NG---AVIKGTEFRLLADAPLDDLVQVPPTR 57 + +GD+VVRKSY D+ FR+ I Q NG AV+ G E RL+ADA L DL V Sbjct: 7 MQFKIGDMVVRKSYRRDILFRIIRIDQSANGEPVAVLHGDEVRLIADAHLGDLEIVREAE 66 Query: 58 ITERGQQAQIKATESLTKLRKDRQEQSQRSGESLTGVWPQSPKEAAYFEVPGKVLHLDGD 117 R ++ + + ESL LR+D + + T Q + YF +PG+VLHLDGD Sbjct: 67 WQMRKREEETRMKESLDLLRQDYKLLHDKHEYRATN---QYNNQQQYFHMPGRVLHLDGD 123 Query: 118 ALYLKKSLSLYEQLRIPAEGHHVHESKMAETLYRLLPHVRPDIVVITGHDGVLKQQHTYD 177 + YLKK L+LYE++ +P G H +E KM+ + L+ RPD++VITGHD KQ+ D Sbjct: 124 SAYLKKCLALYEKIGVPVYGIHCYEKKMSSVIEELIDEYRPDLLVITGHDAYSKQKG--D 181 Query: 178 LYSLNSYKNSQNFVTAVKVAREYERNFDTLXXXXGGGPCQSHFEALLGAGANFASSPGRI 237 + +L++Y++S++F+ V+ AR + D L G CQSHFE+L+ AGANFASSP R+ Sbjct: 182 INNLDAYRHSKDFIETVQKARRKIPHLDQLVIF--AGACQSHFESLIRAGANFASSPSRV 239 Query: 238 LIHALDPVYIAAKASFTSIRDSVSLGDVLNNTISGSQGMGGIETRGSFRIGMP 290 IHALDPVYI AK SFT D +++ +VL NT++ +G+GGIETRG RIGMP Sbjct: 240 NIHALDPVYIVAKISFTPFMDRINVWEVLRNTLTREKGLGGIETRGVLRIGMP 292 >A5A6A5.1 A5A6A5_BACLD Spore coat assembly Peptidase U57, YabG Length = 293 Score = 247 bits (631), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 134/293 (45%), Positives = 185/293 (63%), Gaps = 12/293 (4%) Query: 3 FEMNLGDLVVRKSYGGDVTFRVENIVQ--NG---AVIKGTEFRLLADAPLDDLVQVPPTR 57 + +GD+VVRKSY D+ FR+ I Q NG AV+ G E RL+ADA L DL V Sbjct: 1 MQFKIGDMVVRKSYRRDILFRIIRIDQSANGEPVAVLHGDEVRLIADAHLGDLEIVREAE 60 Query: 58 ITERGQQAQIKATESLTKLRKDRQEQSQRSGESLTGVWPQSPKEAAYFEVPGKVLHLDGD 117 R ++ + + ESL LR+D + + T Q + YF +PG+VLHLDGD Sbjct: 61 WQMRKREEETRMKESLDLLRQDYKLLHDKHEYRATN---QYNNQQQYFHMPGRVLHLDGD 117 Query: 118 ALYLKKSLSLYEQLRIPAEGHHVHESKMAETLYRLLPHVRPDIVVITGHDGVLKQQHTYD 177 + YLKK L+LYE++ +P G H +E KM+ + L+ RPD++VITGHD KQ+ D Sbjct: 118 SAYLKKCLALYEKIGVPVYGIHCYEKKMSSVIEELIDEYRPDLLVITGHDAYSKQKG--D 175 Query: 178 LYSLNSYKNSQNFVTAVKVAREYERNFDTLXXXXGGGPCQSHFEALLGAGANFASSPGRI 237 + +L++Y++S++F+ V+ AR + D L G CQSHFE+L+ AGANFASSP R+ Sbjct: 176 INNLDAYRHSKDFIETVQKARRKIPHLDQLVIF--AGACQSHFESLIRAGANFASSPSRV 233 Query: 238 LIHALDPVYIAAKASFTSIRDSVSLGDVLNNTISGSQGMGGIETRGSFRIGMP 290 IHALDPVYI AK SFT D +++ +VL NT++ +G+GGIETRG RIGMP Sbjct: 234 NIHALDPVYIVAKISFTPFMDRINVWEVLRNTLTREKGLGGIETRGVLRIGMP 286 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,744,782,105 Number of extensions: 69785450 Number of successful extensions: 179227 Number of sequences better than 1.0e-05: 156 Number of HSP's gapped: 178733 Number of HSP's successfully gapped: 156 Length of query: 306 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 171 Effective length of database: 1,788,859,178 Effective search space: 305894919438 Effective search space used: 305894919438 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)