BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0026.p (519 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4Q7.1 B3K4Q7_9BACI Metal-dependent phosphohydrolase HD sub do... 584 e-165 B1YFQ8.1 B1YFQ8_EXIS2 Metal dependent phosphohydrolase 444 e-123 C1PTK3.1 C1PTK3_ALIAC HD-GYP domain-containing protein 443 e-122 B7DM79.1 B7DM79_9BACL Metal dependent phosphohydrolase 442 e-122 A8VQ83.1 A8VQ83_9BACI Putative metal dependent phosphohydrolase 419 e-115 >B3K4Q7.1 B3K4Q7_9BACI Metal-dependent phosphohydrolase HD sub domain Length = 501 Score = 584 bits (1506), Expect = e-165, Method: Compositional matrix adjust. Identities = 303/499 (60%), Positives = 359/499 (71%), Gaps = 3/499 (0%) Query: 10 MNLYGGFLKKQIRNYIFGSVVAVLAVGXXXXXXXXXXXTVEYFRLLIILLISFAIMAVVE 69 M LY FLK+ IRNY+ GS+ AVL VG T E RL++IL ISF +M E Sbjct: 1 MQLYKSFLKQLIRNYMIGSIAAVLVVGGVLMVTTLEIPTSEGVRLMLILAISFMVMVASE 60 Query: 70 ISVFIRQIRPIRAALTEHRPALAMLEEAYLHTHRLPKLAVQRILGPHFLGLSLPASVMTY 129 ++VF+R +RPI A E L LE+AYL HR+P+LAV RI GPH LGLSLPA ++T Sbjct: 61 LTVFLRHLRPIHAGFNEGHTDLETLEKAYLSIHRMPRLAVYRIFGPHLLGLSLPAILLTV 120 Query: 130 GMIRTGLINLPYFYXXXXXXXXXXXXCMHALIEFFLTSSAIIPLIKNFRNRALELHMVDF 189 M+ G +N P FY HA+IEFFLT +AI PLIK R +AL + VDF Sbjct: 121 WMMEQGKLNFPPFYIWLACLGAVLLASCHAMIEFFLTIAAIRPLIKEVRRQALSRYGVDF 180 Query: 190 SLEGHVFVPIRPKFLITCMLIGTFPLVLFIMATQIRLETLGGRAAESMRSYWEWAGLVLL 249 SLEGHVF+ IR KFL++ MLIGTFPL LF +A QIRLE L A+ YW WAG +LL Sbjct: 181 SLEGHVFMAIRTKFLLSTMLIGTFPLFLFSLAVQIRLEGLSQIVAQQ---YWGWAGFILL 237 Query: 250 IGTLFSSIAAWLLTRSVQHPINELYRAMNNIKDGRLLQVQDEYSDEFSKLVAGFNMMVRG 309 +G F+ I AWLLT+ +Q PI +LYRAMN IK+GRL+Q + YSDEFS L+AGFNMMVRG Sbjct: 238 LGVGFAYIGAWLLTQDLQRPIRQLYRAMNEIKEGRLIQTSNLYSDEFSNLIAGFNMMVRG 297 Query: 310 LQAREQQSRQLLDSYFTTLAVALDARDPYTAGHSLRVAEYSVIIGQLAGLTGQQLDNLRK 369 LQ RE+++R+LLDSYF LA ALDARDPYTAGHSLRVAEYSV+IG+LAGL+ +Q+D L K Sbjct: 298 LQVREERNRKLLDSYFAALAAALDARDPYTAGHSLRVAEYSVLIGRLAGLSEEQVDLLHK 357 Query: 370 SALLHDIGKIGVRDSVLFKXXXXXXXXFDQIKSHPVLGENILRQIEPEEKMAPYLGGVRS 429 +ALLHDIGKIGVRD++L K FDQ+K+HP GENIL QIEP + MAPYL GVRS Sbjct: 358 TALLHDIGKIGVRDNILLKEGKLTAEEFDQVKAHPAQGENILLQIEPADAMAPYLEGVRS 417 Query: 430 HHERYDGKGYPDGLAGTAIPLHGRIIAVADAYDAMTSDRPYRKGMDHDRALAILEQGRGT 489 HHERYDG GYPDGL G IPL GRIIAVADAYDAMTSDRPYR GM AL+ILE GRGT Sbjct: 418 HHERYDGGGYPDGLKGKEIPLFGRIIAVADAYDAMTSDRPYRSGMKSADALSILEAGRGT 477 Query: 490 QWDPEFAGLFLAYFGKTGK 508 QWDP++AG+F+ Y K Sbjct: 478 QWDPDYAGMFVKYMKAEKK 496 >B1YFQ8.1 B1YFQ8_EXIS2 Metal dependent phosphohydrolase Length = 497 Score = 444 bits (1143), Expect = e-123, Method: Compositional matrix adjust. Identities = 225/485 (46%), Positives = 307/485 (63%), Gaps = 5/485 (1%) Query: 16 FLKKQIRNYIFGSVVAVLAVGXXXXXXXXXXXTVEYFRLLIILLISFAIMAVVEISVFIR 75 F ++ ++NY+ GS VAV VG E LL I+ +S +M E +++ + Sbjct: 7 FERQLLKNYLIGSFVAVFGVGCLFIFETLTFDARERVTLLTIMFLSVVLMFSFEYTIYRK 66 Query: 76 QIRPIRAALTEHRPALAMLEEAYLHTHRLPKLAVQRILGPHFLGLSLPASVMTYGMIRTG 135 +RP+ P+ A L A+ THR P L ++RILGPHFLGLS+P+S +T I Sbjct: 67 HMRPLYQFFQTSTPSQAQLTAAFRTTHRFPLLTIKRILGPHFLGLSIPSSSLTALAIHFQ 126 Query: 136 LINLPYFYXXXXXXXXXXXXCMHALIEFFLTSSAIIPLIKNFRNRALELHMVDFSLEGHV 195 + +PY+ +HALIEFFLT ++ ++ + + L Sbjct: 127 WLEMPYYLIGLACFGAILVAILHALIEFFLTYRVTESMLAELTVQSQQYGHGELILTKKD 186 Query: 196 FVPIRPKFLITCMLIGTFPLVLFIMATQIRLETLGGRAAESMRSYWEWAGLVLLIGTLFS 255 F+ +R K LI+ ++IG FP++LF++A+ ++L ES+RSYW W+ L+L++ + Sbjct: 187 FISLRKKMLISTLIIGVFPILLFVLASAVQL-----TENESLRSYWSWSTLILIVILCLA 241 Query: 256 SIAAWLLTRSVQHPINELYRAMNNIKDGRLLQVQDEYSDEFSKLVAGFNMMVRGLQAREQ 315 + + LL ++Q PI L + I+ G+L + + +SDEFS+LV GFN+MV G+Q R+Q Sbjct: 242 TFCSLLLYENIQKPILALQEGVAQIESGQLNTINNPFSDEFSQLVGGFNLMVEGIQGRDQ 301 Query: 316 QSRQLLDSYFTTLAVALDARDPYTAGHSLRVAEYSVIIGQLAGLTGQQLDNLRKSALLHD 375 ++ QLLDS FT A LDARDPYTAGHSLRVAEYSV I + AGL Q++ LRKSALLHD Sbjct: 302 ENEQLLDSLFTLFAATLDARDPYTAGHSLRVAEYSVEIARAAGLADDQVELLRKSALLHD 361 Query: 376 IGKIGVRDSVLFKXXXXXXXXFDQIKSHPVLGENILRQIEPEEKMAPYLGGVRSHHERYD 435 IGKIG+RD VL K FD+IK HPV+G +IL Q+ EK+ P L GV+ HHERYD Sbjct: 362 IGKIGIRDDVLLKEGRLTGEEFDKIKQHPVIGVHILSQVHLPEKLQPILPGVKYHHERYD 421 Query: 436 GKGYPDGLAGTAIPLHGRIIAVADAYDAMTSDRPYRKGMDHDRALAILEQGRGTQWDPEF 495 GKGYP+GLAG +IP+ GRI+A+ADAYDAMTSDRPYRKGM +ALAILE+G GTQWD F Sbjct: 422 GKGYPEGLAGESIPVFGRIMAIADAYDAMTSDRPYRKGMPVAKALAILEEGSGTQWDASF 481 Query: 496 AGLFL 500 LFL Sbjct: 482 TQLFL 486 >C1PTK3.1 C1PTK3_ALIAC HD-GYP domain-containing protein Length = 518 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 233/491 (47%), Positives = 302/491 (61%), Gaps = 9/491 (1%) Query: 16 FLKKQIRNYIFGSVVAVLAVGXXXXXXXXXXXTVEYFRLLIILLISFAIMAVVEISVFIR 75 +K+ I NY GSV+AVL VG + + + IL S +M + F Sbjct: 12 LVKRLILNYFAGSVIAVLGVGGVLIFTTLHLSQEDLWVICGILGGSLVLMLTADSIAFRV 71 Query: 76 QIRPIRAALTEHRPALAMLEEAYLHTHRLPKLAVQRILGPHFLGLSLPASVMTYGMIRTG 135 QIRPIR AL P +L + Y LP LAV R++GPH+L +P V + ++R G Sbjct: 72 QIRPIRKALLTAHPTEDVLAKGYKRALHLPALAVLRVMGPHWLSFLIPGLVSSSILVRRG 131 Query: 136 LINLPYFYXXXXXXXXXXXXCMHALIEFFLTSSAIIPLIKNFRNRALELHMVDFSLEGHV 195 L++LP Y CMHA+IEFFLTS +I P + R R+ ++ L G V Sbjct: 132 LLHLPMAYVWLASLGTLIVACMHAVIEFFLTSRSIEPTLVTIRRRSEHMYGASVLLRGQV 191 Query: 196 FVPIRPKFLITCMLIGTFPLVLFIMATQIRL--ETLGGRAAESMRSYWEWAGLVLLIGTL 253 VP+ KF ++ ++ G PL+LF +A +RL E LG +YW WAG +L +G+L Sbjct: 192 VVPLAFKFAMSAVVFGALPLLLFGLANAVRLTHEGLGSS------TYWTWAGGILALGSL 245 Query: 254 FSSIAAWLLTRSVQHPINELYRAMNNIKDGRL-LQVQDEYSDEFSKLVAGFNMMVRGLQA 312 FS+ +LL VQ PI L M ++ G L+ D Y DEFS L+ GFN+M+ GL Sbjct: 246 FSAFGGYLLASDVQRPIRRLEALMRRVERGDFSLRADDTYMDEFSDLIQGFNLMLNGLAH 305 Query: 313 REQQSRQLLDSYFTTLAVALDARDPYTAGHSLRVAEYSVIIGQLAGLTGQQLDNLRKSAL 372 R+ +RQL+DSYF TLA ALDARDPYTAGHS RVA Y+ IG+ L+ Q + LR+SAL Sbjct: 306 RDAMNRQLIDSYFATLAAALDARDPYTAGHSQRVARYAEAIGRKVNLSPQTVRELRQSAL 365 Query: 373 LHDIGKIGVRDSVLFKXXXXXXXXFDQIKSHPVLGENILRQIEPEEKMAPYLGGVRSHHE 432 LHDIGKIG+RD +L K F IK HPV+GENI+RQI+P++ M P L G+RSHHE Sbjct: 366 LHDIGKIGIRDEILLKEGKLTDEEFAIIKQHPVIGENIIRQIQPDDAMKPLLPGIRSHHE 425 Query: 433 RYDGKGYPDGLAGTAIPLHGRIIAVADAYDAMTSDRPYRKGMDHDRALAILEQGRGTQWD 492 RYDGKGYPDGLAG IPL GRI+AVADA+DAMTSDRPYR GM +RA+ +L +GRGTQWD Sbjct: 426 RYDGKGYPDGLAGEEIPLFGRILAVADAFDAMTSDRPYRSGMPVERAIQVLREGRGTQWD 485 Query: 493 PEFAGLFLAYF 503 P F F+ + Sbjct: 486 PHFVDAFIEVY 496 >B7DM79.1 B7DM79_9BACL Metal dependent phosphohydrolase Length = 518 Score = 442 bits (1138), Expect = e-122, Method: Compositional matrix adjust. Identities = 232/491 (47%), Positives = 302/491 (61%), Gaps = 9/491 (1%) Query: 16 FLKKQIRNYIFGSVVAVLAVGXXXXXXXXXXXTVEYFRLLIILLISFAIMAVVEISVFIR 75 +K+ I NY GSV+AVL VG + + + IL S +M + F Sbjct: 12 LVKRLILNYFAGSVIAVLGVGGVLIFTTLHLSQEDLWIICCILGGSLVLMLTADSIAFRA 71 Query: 76 QIRPIRAALTEHRPALAMLEEAYLHTHRLPKLAVQRILGPHFLGLSLPASVMTYGMIRTG 135 QIRPIR AL P +L + Y LP LAV R++GPH+L +P V + ++R G Sbjct: 72 QIRPIRKALLTAHPTEDVLAKGYQRALHLPALAVLRVMGPHWLSFLIPGLVSSSILVRRG 131 Query: 136 LINLPYFYXXXXXXXXXXXXCMHALIEFFLTSSAIIPLIKNFRNRALELHMVDFSLEGHV 195 L++LP Y CMHA+IEFFLTS +I P + R R+ ++ L G V Sbjct: 132 LLHLPMAYVWLASLGTLIVACMHAVIEFFLTSRSIEPTLLTIRRRSEHMYGASVWLRGQV 191 Query: 196 FVPIRPKFLITCMLIGTFPLVLFIMATQIRL--ETLGGRAAESMRSYWEWAGLVLLIGTL 253 VP+ KF ++ ++ G PL+LF +A +RL E LG +YW WAG +L +G+L Sbjct: 192 VVPLAFKFAMSAVVFGALPLLLFGLANAVRLTHEGLGSS------TYWTWAGGILALGSL 245 Query: 254 FSSIAAWLLTRSVQHPINELYRAMNNIKDGRL-LQVQDEYSDEFSKLVAGFNMMVRGLQA 312 FS+ +LL VQ PI L M ++ G L+ D Y DEFS L+ GFN+M+ GL Sbjct: 246 FSAFGGYLLASDVQRPIRRLEALMRRVERGDFSLRADDTYMDEFSDLIQGFNLMLNGLAH 305 Query: 313 REQQSRQLLDSYFTTLAVALDARDPYTAGHSLRVAEYSVIIGQLAGLTGQQLDNLRKSAL 372 R+ +RQL+DSYF TLA ALDARDPYTAGHS RVA Y+ IG+ L+ Q + LR+SAL Sbjct: 306 RDAMNRQLIDSYFATLAAALDARDPYTAGHSQRVARYAEAIGRKVNLSPQTVRALRQSAL 365 Query: 373 LHDIGKIGVRDSVLFKXXXXXXXXFDQIKSHPVLGENILRQIEPEEKMAPYLGGVRSHHE 432 LHDIGKIG+RD +L K F IK HPV+GENI+RQI+P++ M P L G+RSHHE Sbjct: 366 LHDIGKIGIRDEILLKEGKLTDEEFAIIKQHPVIGENIIRQIQPDDAMKPLLPGIRSHHE 425 Query: 433 RYDGKGYPDGLAGTAIPLHGRIIAVADAYDAMTSDRPYRKGMDHDRALAILEQGRGTQWD 492 RYDGKGYPDGLAG IPL GRI+AVADA+DAMTSDRPYR GM ++A+ +L +GRGTQWD Sbjct: 426 RYDGKGYPDGLAGEEIPLFGRILAVADAFDAMTSDRPYRAGMPVEKAIQVLREGRGTQWD 485 Query: 493 PEFAGLFLAYF 503 P F F+ + Sbjct: 486 PHFVDAFIEVY 496 >A8VQ83.1 A8VQ83_9BACI Putative metal dependent phosphohydrolase Length = 486 Score = 419 bits (1078), Expect = e-115, Method: Compositional matrix adjust. Identities = 219/501 (43%), Positives = 318/501 (63%), Gaps = 16/501 (3%) Query: 10 MNLYGGFLKKQIRNYIFGSVVAVLAVGXXXXXXXXXXXTVEYFRLLIILLISFAIMAVVE 69 M+L F + + NY+ GS++AV VG + E LL I+++S IM +E Sbjct: 1 MDLLNQFHVRMLINYMVGSLIAVFGVGSVFIFHTLTLSSEETLILLGIMVLSGLIMISLE 60 Query: 70 ISVFIRQIRPIRAALTEHRPALAMLEEAYLHTHRLPKLAVQRILGPHFLGLSLPASVMTY 129 + V+ + IRP+ + ++AY+ H P + V+RI+GPH LGLS+PA+VMT Sbjct: 61 LFVYSKHIRPLVHFFKKGMNP----QDAYMTAHSFPFMTVRRIMGPHLLGLSIPATVMTL 116 Query: 130 GMIRTGLINLPYFYXXXXXXXXXXXXCMHALIEFFLTSSAIIPLIKNFRNRALELHMVDF 189 + G + LP+ Y +HA+IEFF+TS A+ PLI + R E + Sbjct: 117 FALSLGFLTLPHLYVLYAWIGAILIATLHAMIEFFMTSRAVQPLIASICARTDE----NL 172 Query: 190 SLEGHVFVPIRPKFLITCMLIGTFPLVLFIMATQIRLETLGGRAAESMRSYWEWAGLVLL 249 L+G + ++ K L++ +LI FP LF++A QIR +AE+ +YW WA LV++ Sbjct: 173 KLDGVKVISMKWKLLLSMLLIAVFPAALFLLAGQIR------ESAETTTAYWNWAFLVIV 226 Query: 250 IGTLFSSIA-AWLLTRSVQHPINELYRAMNNIKDGRLLQVQDEYSDEFSKLVAGFNMMVR 308 + LF SIA A +L R+++ PI EL + + ++ G ++ + Y+DEFS L+ GFN MV Sbjct: 227 V-ILFVSIAGAVVLYRNMEQPILELRKNLEQVQHGEFHEMPNYYADEFSTLINGFNSMVS 285 Query: 309 GLQAREQQSRQLLDSYFTTLAVALDARDPYTAGHSLRVAEYSVIIGQLAGLTGQQLDNLR 368 G+++R+ ++ +LL+S+F+ A LDARD YTAGH+ RVA+YS I + +GL+G +LD LR Sbjct: 286 GIKSRDAENERLLESFFSVFAATLDARDSYTAGHTTRVADYSTQIARRSGLSGDELDLLR 345 Query: 369 KSALLHDIGKIGVRDSVLFKXXXXXXXXFDQIKSHPVLGENILRQIEPEEKMAPYLGGVR 428 KSALLHDIGKIG+ DSVL K FD+IK HPV+G +IL ++ E + P L GVR Sbjct: 346 KSALLHDIGKIGIPDSVLLKDGKLTDEEFDKIKEHPVIGGDILEKVNLPEHLRPLLDGVR 405 Query: 429 SHHERYDGKGYPDGLAGTAIPLHGRIIAVADAYDAMTSDRPYRKGMDHDRALAILEQGRG 488 HHER+DGKGYPDGL+G IPL+GRIIAVADA+DAMTS+RPYRK M +++A IL+ GRG Sbjct: 406 HHHERFDGKGYPDGLSGDRIPLYGRIIAVADAFDAMTSNRPYRKAMSYEQARNILQNGRG 465 Query: 489 TQWDPEFAGLFLAYFGKTGKS 509 +QWDP F F+ ++ + K+ Sbjct: 466 SQWDPAFIDHFMDFYEERFKT 486 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.142 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,816,473,654 Number of extensions: 110806947 Number of successful extensions: 382238 Number of sequences better than 1.0e-05: 2508 Number of HSP's gapped: 379701 Number of HSP's successfully gapped: 2532 Length of query: 519 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 379 Effective length of database: 1,741,754,103 Effective search space: 660124805037 Effective search space used: 660124805037 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)